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Bambang Sugiharto
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember
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Overexpression Sucrose Transporter Protein (Sut) and Sucrose Content In Genetically Modified Product (Gmp) Sugarcane(Saccharum officinarum L.) Dewanti, Parawita; Okviandari, Purnama; Oktaria, Nina; Sugiharto, Bambang
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Sucrose transporter (SUT) is a protein that functions to translocate sucrose from source to sink. Protein activity is determined by the accumulation of sucrose in sugarcane stem. The level of sucrose accumulation is also influenced by the activity of  sucrose phosphat synthase (SPS) and neutral invertase (NI) in conjunction with the synthesis and hydrolysis of sucrose. This aim of this study is to determine the sucrose transporter protein expression in Genetically Modified Product (GMP) of sugarcane.Genetically Modified Product GMP sugarcane events 1, 2, 3, 4, 5, 18, 20 and non GMP were used as materials. The analysis was performed by Western Blot method to observe the presence of SUT protein expression, stem sucrose content by resorcinol method to observe the translocation of sucrose, the SPS enzyme activity and NI that play a role in determining the accumulation of sucrose.The results showed that (1) an increase in SUT protein content in GMP plants events 2 and 18 with a protein band that is thicker than the non GMP plants, (2) SPS and invertase activity at all events GMP crop plants to increase compared to non GMP. Sucrose content of the stem at all events GMP plant to increase with age segment. The older age segments, the higher the sucrose  content. Keywords: sugarcane (Saccharum officinarum L.), genetically modified product(GMP), sucrose transporter protein(SUT), sucrose
Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agung Nur Probohudono Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Hidayat Ahmad Ilham Tanzil Ai Sumirah Setiawati Amir, Muhammad Hisyam Andriani, Maya Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Asshidiqie, Wahyu Pratama Azizah, Kunti Anis Bambang Sugiharto Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dr. Hadrianus Tedjoworo, S.Ag., STL. Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edison Edison Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Fibriani, Suwinda Gaol, Nickytha Linch De Hans Lumban Gulo, Desi Natalia Hafiz, Syauqi Abyan Hafizi, Muhamad Halawa, Herry Martinus Hari Purnomo Harkim, H. Harkim, Harkim Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Hiya, Nirmadarningsih Husin, Andriati Amir Icih, Icih Ika Oktavianawati Ilhamsyah Siregar Imas Cintamulya Insani, Vivian Nurul Intan Ria Neliana Inyana Dwi Agustien Irawan Gulo, Sastra Darma Iryani Endah Febrianti Jayus Jayus Kesuma, Muhammad Yudha Kurnia Indriyanti Purnamasari, Kurnia Indriyanti Kurniawan , Asep Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Lathifah Hanum, Lathifah Liem, Jhon Sepron Defretus Lina Maulidiana Maesaroh, Nurlaila Maisaro, Maisaro Marina Pakaya Maulani, Ardela Alief MAYA ANDRIANI Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Mohd Radzi, Fathin Nuril A'isha Binti Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nirwana Br Bangun Nugroho, Sandi Luqman Nurfatimah, Siti Nurhayati Nursely, Dinda Oktapan, Ladefa Primana Parawita Dewanti Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Putri, Trisandi Eka Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhan, Mohamad Ibnu Ramadhani, Rifki Fahrisal Ratnasari, Tri Ratri Ciptaningtyas Rejekia V Simanungkalit Restanto, Didik Pudji Rizal, Asep Rizki, Ainur Salahuddin Junus Salih, Yasir Sigit Soepardjono Simamora, Harkim Simanungkalit, Rejekia Vaizal Sirait, Syah Paradiba Siti Arifah Siti Rohimah Siti Umayyah Soleh, Mohamad Badrus SRI AYU MADE Sri Mulyati Sri Mulyati Sri Panca Setyawati Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sukardiman Syafina Pusparani Tanjung, Yulia Tiara Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Ummi Sholikhah Untung Murdiyanto Wahyu Nurkholis Hadi Syahputra Widhi Dyah Sawitri Widhiya Ninsiana, Widhiya Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto YULIANINGSIH, LILIK Yundari, Yundari Yusuf Rachmandika