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All Journal Vegetalika AGRITROP International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat ROTOR: JURNAL ILMIAH TEKNIK MESIN Indonesian Journal of Biotechnology UNEJ e-Proceeding Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Informasi dan Teknologi Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Buletin Penelitian Tanaman Rempah dan Obat Agroradix : Jurnal Ilmu Pertanian International Journal of Quantitative Research and Modeling BIOVALENTIA: Biological Research Journal Journal of Law, Education and Business
Bambang Sugiharto
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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Journal : unej e-proceeding

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Overexpression Sucrose Transporter Protein (Sut) and Sucrose Content In Genetically Modified Product (Gmp) Sugarcane(Saccharum officinarum L.) Dewanti, Parawita; Okviandari, Purnama; Oktaria, Nina; Sugiharto, Bambang
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Sucrose transporter (SUT) is a protein that functions to translocate sucrose from source to sink. Protein activity is determined by the accumulation of sucrose in sugarcane stem. The level of sucrose accumulation is also influenced by the activity of  sucrose phosphat synthase (SPS) and neutral invertase (NI) in conjunction with the synthesis and hydrolysis of sucrose. This aim of this study is to determine the sucrose transporter protein expression in Genetically Modified Product (GMP) of sugarcane.Genetically Modified Product GMP sugarcane events 1, 2, 3, 4, 5, 18, 20 and non GMP were used as materials. The analysis was performed by Western Blot method to observe the presence of SUT protein expression, stem sucrose content by resorcinol method to observe the translocation of sucrose, the SPS enzyme activity and NI that play a role in determining the accumulation of sucrose.The results showed that (1) an increase in SUT protein content in GMP plants events 2 and 18 with a protein band that is thicker than the non GMP plants, (2) SPS and invertase activity at all events GMP crop plants to increase compared to non GMP. Sucrose content of the stem at all events GMP plant to increase with age segment. The older age segments, the higher the sucrose  content. Keywords: sugarcane (Saccharum officinarum L.), genetically modified product(GMP), sucrose transporter protein(SUT), sucrose
Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Ilham Tanzil Ahmad Ilham Tanzil Amir, Muhammad Hisyam Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Arsy Chairiyah Asshidiqie, Wahyu Pratama Azizah, Kunti Anis Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Ferdi Hariyanto Fibriani, Suwinda Hafiz, Syauqi Abyan Hafizi, Muhamad HARDIAN SUSILO ADDY Hari Purnomo Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Husin, Andriati Amir Ida Ayu Putri Darmawanti Ika Oktavianawati Imas Cintamulya Inyana Dwi Agustien Iryani Endah Febrianti Jayus Jayus Khiar Ayatina Hasbi Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Liem, Jhon Sepron Defretus Maisaro, Maisaro Maulani, Ardela Alief Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Muslimah Arniyanti Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Ni Putu Ayu Erninda Oktaviani Suputri Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nugroho, Sandi Luqman Nurhayati Oktapan, Ladefa Primana Parawita Dewanti Purnama Okviandari Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhani, Rifki Fahrisal Ratnasari, Tri Raudhotun Jamila Restanto, Didik Pudji Retnosari Apriasti Ridho Dwi Ramadhani Rindang Dwiyani Risky Mulana Anur Salahuddin Junus Salih, Yasir Sholeh Avivi Sigit Soepardjono Siswahyudianto Siti Rohimah Siti Umayyah Soleh, Mohamad Badrus SRI AYU MADE Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sukardiman Syafina Pusparani Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Tri Ratnasari Ummi Sholikhah Ummi Sholikhah Untung Murdiyanto Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Wahyu Nurkholis Hadi Syahputra Weny Nailul Hidayati Widhi Dyah Sawitri Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto Yundari, Yundari Yusuf Rachmandika