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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Disease Prevention and Public Health Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Jurnal Teknologi Laboratorium Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis Journal of Public Health and Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Tamilis Synex: Multidimensional Collaboration
Nanik Sulistyani
Fakultas Farmasi, Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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AKTIVITAS ANTIKANKER EKSTRAK ETANOL TANAMAN TAPAK LIMAN (Elephantopus scaber L.) TERHADAP SEL T47D MELALUI INDUKSI APOPTOSIS Panji Ratih Suci; Nurkhasanah Nurkhasanah; Nanik Sulistyani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 1 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.223 KB) | DOI: 10.36387/jiis.v6i1.605

Abstract

Breast cancer is the most dominant type of cancer in Indonesia. Traditional medicine using tapak liman (Elephantopus scaber L.) as an anticancer becomes an option because it contains compounds such as sesquiterpenes. The purpose of this study was to know the cytotoxic activity of ethanolic extract of tapak liman on T47D cells through induction of apoptosis. The extraction method uses maceration with 96%. Total flavonoids were tested using quercetin as a spectrophotometric standard. ethanol solvent, cytotoxic test using microtetrazoliom (MTT assay), and apoptosis test using double staining test (ethidium bromide-acrydine orange) under a fluorescence microscope. The results showed the linear regression equation y = 0.0934x-0.0292 with the resulting flavonoid levels of 0.979% w / w calculated against quercetin (QE) The concentration of the compound used was 1000 µg/ml; 500 µg/ml; 250µg/ml; 125 ?g/ml, 62.5 ?g/ml; 31.25 µg/ml. The MTT assay results are the values of IC50 which are analyzed using probit analysis and apoptosis tests which are analyzed descriptively. The results of the research show that the ethanol extract of tapak liman using MTT method has IC50 value of 59 µg/ml, while the testing of double staining method shows the color of bright green which indicates early apoptosis and the color orange in the cells which undergoing final apoptosis. DNA fragmentation results shows that Tapak Liman extract induces DNA fragments. The conclusion of this study is that tapak liman textract has the potential for cytotoxic activity against T47D breast cancer cells through the induction of apoptosis
Culture Broth Activity of Antibiotic Producer Bacteria (P301 Isolate) Against Staphylococcus aureus ATCC 25923 and Time Optimization of Secondary Metabolite Production NANIK SULISTYANI; IIN NARWANTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1138.691 KB)

Abstract

Isolat P301 adalah bakteri penghasil antibiotik yang diisolasi dari rizosfer tanaman padi. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antibakteri dari cairan kultur isolat P301 terhadap Staphylococcus aureus ATCC 25923 dan mengetahui profil optimasi waktu produksi metabolit sekunder dari isolat P301 sebagai penghasil antibiotik. Aktivitas antibakteri ditetapkan dengan metode sumuran dengan mengukur zona hambat di sekitar sumuran. Penentuan profil optimasi waktu produksi metabolit sekunder yaitu dengan membuat grafik hubungan antara diameter zona hambat dengan waktu inkubasi. Hasil penelitian menunjukkan bahwa cairan kulturisolat P301 mampu menghambat pertumbuhan S. aureus. Berdasarkan uji aktivitas terhadap S. aureus, waktu produksi antibiotik yang optimal adalah setelah diinkubasi minimal selama 11 hari.
Aktivitas Antibiotik Isolat Bakteri Kp13 dan Analisa Kebocoran Sel Bakteri Escherichia coli Alfian Syarifuddin; Nanik Sulistyani; Kintoko Kintoko
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 2 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.405 KB) | DOI: 10.35814/jifi.v16i2.529

Abstract

A bacterium is one of microorganisms that can produce secondary metabolite, such as antibiotic. KP13 isolate is a bacterium isolated from Melaleucaleuca dendron L rhizosphere. The aim of this study is to know the eff ect of the most active fraction antibacterium of KP13 isolate toward Escherichia coli bacterium and the ability to caused cell leakage. Extraction of antibiotic was conducted by ethyl acetate. Extract is fractioned with a n-heksan-etilacetate solvent gradient another uses a solvent methanol. Each fraction is done by KLT and grouped based on RF value. A fraction group is compare by inhibition eff ectiveness. Then, the Minimum Inhibitory Concentration (MIC) from the most active group is tested. Cell leakage analysis was performed using UV spectrophotometry to detect the release of nucleic acid and protein. Atomic Absorption Spectrophotometry (AAS) was used to detect ion release of K+ and Ca2+ from. cell of bacteria. The results showed that the most active fraction against Escherichia coli was the fraction number 1 with MIC value of 5% and inhibition zone of 7.33±0.58 mm.
Aktivitas Isolat Actinomycetes dari Rumput Laut (Eucheuma cottonii) sebagai Penghasil Antibiotik terhadap Staphylococcus aureus dan Escherichia coli NANIK SULISTYANI; ACHMAD NURYADIN AKBAR
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (927.173 KB)

Abstract

Actinomycetes are microbial group of the most widely produced bioactive compound as antibiotics.This study was intended to isolate Actinomycetes from seaweed Eucheuma cottonii and to identify the activity of isolated Actinomycetes as antibiotic producer against Staphylococcus aureus and Escherichia coli. Furthermore, the research was aimed to know the spots on thin-layer chromatography(TLC) which has antibacterial activity against S. aureus and E. coli. Isolation of Actinomycetes from E. cottonii was carried out with a spread plate method. The activity test of Actinomycetes as antibiotic producer against S. aureus and E. coli was performed by cup plate method. TLC bioautography was done with stationary phaseof silica gel GF254 and mobile phase of n-hexane : ethylacetate (2:3). The results showed that RL 6 and RL 12 isolate produced antibiotic against S. aureus and E. coli.The TLC bioautography result of ethyl acetate extracts of RL 6 and RL 12 cultures against S. aureus and E. coli showed a inhibition zone at Rf 0.05 and 0.22, respectively.
The antioxidant activity of several antidiabetic herbal products Ichwan Ridwan Rais; Andhika Septiawan; Meta Ayuni; Dhega Agung Wichaksono; Nanik Sulistyani
Pharmaciana Vol 12, No 2 (2022): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.825 KB) | DOI: 10.12928/pharmaciana.v12i2.22714

Abstract

The oxidative stress mechanism in the body involves the balance between increased ROS and decreased antioxidant agents. ROS can damage DNA, proteins, and lipids, leading to pathology and impaired insulin secretion by dysregulation of several genes expression. High concentrations of ROS are often followed by a low activity of antioxidants from endogenous sources. As a result, exogenous antioxidants are required to neutralize ROS. As reported, medicinal plants, which have had various traditional applications, contain large amounts of secondary metabolites, proven to be exogenous antioxidant agents. Jamu, a traditional medicine, also known as an alternative medication, can be widely and easily found in traditional markets. Antidiabetic herbal products are among the most popular of jamu. Antidiabetic mechanism has a strong relationship with antioxidant roles in many biological systems. The aim of this research was to figure out the potential antioxidant activity of antidiabetic herbal products. Eight antidiabetic jamu samples were analyzed for their antioxidant activity by measuring the DPPH radical scavenging activity, phosphomolybdenum antioxidant activity, and FRAP, as well as for their total phenolic and flavonoid contents. Good linearity was a general finding for the quantitative analysis, with an average correlation coefficient of 0.999 for the standards. The highest total flavonoid content found was 8.66 ± 0.11 mgQE/g, and the highest total phenolic content was 22.66 ± 0.15 mgGAE/g. With regard to antioxidant activity, all of the samples demonstrated weak DPPH radical scavenging activity, while phosphomolybdenum antioxidant activity and FRAP were the highest at 48.58 ± 0.45 mgQE/g extract and 226.98 ± 0.19 mgQE/g extract, respectively. These results indicated that the claimed antidiabetic herbal products could be prepared as exogenous antioxidant sources, irrespective of being with or without antioxidant activity, for diabetes treatment.
Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy Ade Irawan; Nurkhasanah Nurkhasanah; Nanik Sulistyani
Jurnal Farmasi Sains dan Praktis Vol 5 No 2 (2019)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v5i2.3002

Abstract

One of the plant that has antibacterial activity is chili leaf (Capsicum frutescens L.). The purpose of this study was to determine the antibacterial activity of ethyl acetate fraction of chili leaf against Streptococcus pyogenes bacteria and conduct bioautography to see compounds that have antibacterial activity. In this study an antibacterial test for ethyl acetate fraction of chili leaf with a concentration of 5%, 10%, 20%, 40%, 80% to Streptococcus pyogenes. From the results of the inhibition zone test 5% ethyl acetate fraction produces an average inhibition zone of 6.3 mm, 10% average inhibition zone 10.3 mm, 20% average inhibition zone of 14 mm, 40% average inhibition zone 18 mm and 80% have 22.7 an average inhibition zone. In the silent phase bioautographic was used test silica gel GF 254 and for the mobile phase was used chloroform : ethyl acetate (3: 7), indicating the presence of inhibition zones on the media which had been inoculated by Streptococcus pyogenes bacteria, namely at RF 0.88. From the research that has been done, the results show that the ethyl acetate fraction of chili leaf has inhibitory zone against Streptococcus pyogenes bacteria and the spotting of compounds which have inhibitory zone has an RF of 0.88.
Development of Bangle Rhizome and Purple Sweet Potato Flour Biscuit and Its in vivo Antioxidant Activity in High-Fat Diet-Induced Rats: Biscuit, hiperlipid and antioxidant Nanik Sulistyani; Nurkhasanah Mahfudh; Rofidah Nur Umar; Muhammad Fathurrachman Mantali
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.05

Abstract

Bangle rhizome and purple sweet potato could be used as functional food to overcome health problems such as hyperlipidemia. The anti-hyperlipidemic, nutritional properties of the above-said root vegetables could be formulated into a more community-preferred food in the form of biscuits. For this reason, this study aims to formulate biscuits from the mixed flour of bangle rhizome and purple sweet potato. Next, the prepared product's antioxidant activity and lipid-lowering properties are tested in vivo in high-fat diet-induced Wistar rats. In this study, bangle rhizome and purple sweet potato were turned into flour and formulated into three types of biscuits comprising different ratios of bangle rhizome and purple sweet potato flour (5:39 % w/w (F1), 3:41 % w/w (F2), and 2:42 % w/w (F3)). The study found that the baked products showed good organoleptic and physical properties, yielding golden- to brown-colored biscuits with a distinctive aroma and vaguely bitter after-taste, with F3 showing the highest hardness (8.94 0.18). The proximate analysis test showed that the biscuits achieved three of the six SNI 01-2973-2011 quality requirements. The best formula (F3) exhibited acceptable in vivo antioxidant catalase (5.12 0.16 U/mL) and glutathione peroxidase activity (64.44 2.11 U/mg) in high-fat diet Wistar rats tested for 28 days. The F3 formula was deemed the best, yielding biscuits with low moisture content and good crispiness. The formulated biscuits increased catalase's antioxidant activity (285.47%) and glutathione peroxidase (265.08%) more than the negative control. Hence, the study demonstrated that bangle rhizome and purple sweet potato-containing biscuits were potentially useful functional foods for improving antioxidant activity in high-fat diet-induced Wistar rats.
UJI KANDUNGAN BAKTERI Escherichia coli DALAM PRODUK OBAT TRADISIONAL YANG DIJUAL DI PASAR BERINGHARJO Ken Zaim Arifin; Nanik Sulistyani
Jurnal Fitofarmaka Indonesia Vol 10, No 1 (2023): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v10i1.883

Abstract

Indonesian traditional medicine has long been known and used by the community in maintaining health and treating illnesses. Identification of Escherichia coli bacteria was chosen because this bacterium is an indicator of sanitation and an unsanitary environment in the process of making traditional medicines. The purpose of this study was to determine the contamination of Escherichia coli bacteria in traditional medicines sold at the Beringharjo Traditional Market. This study was an experimental study. The sampling technique in this study was purposive sampling. culture technique on Echerichiae coli bacteria using TBX Agar Media and the Total Plate Number test using PCA. Bacterial analysis can be done by observing the culture media and test media on traditional medicine samples. The results of the ALT values were 70 ± 14,1 x 101  CFU/ml to 14,9 ± 1,2  x 105  CFU/ml and the Escherichia Coli Identification Test was negative or 0 colonies/g. the specified conditions refer to PerBPOM No. 32 of 2019. It can be concluded that in the ALT test of ten products, 80% of them have met the requirements and 20% of them do not meet the requirements. In the Escherichia Coli Identification Test, out of ten samples, all 100% of the samples met the requirements.
Identifikasi Variasi Gen dan Ekspresi Gen Yang Berhubungan Dengan Anemia Aplastik Menggunakan Pendekatan Genomik dan Bioinformatika Ayu Lifia Nur Kartikasari; Anisa Devi Kharisma Wibowo; Henry Budiawan Prasetya; La Malihi; Nanik Sulistyani; Lalu Muhammad Irham; Wirawan Adikusuma; Abdi Wira Septama; Riat El Khair; Rahmat Dani Satria
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 4, No 2 (2023): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v4i2.13335

Abstract

Anemia aplastik merupakan anemia yang disertai oleh pansitopenia pada tepi darah yang disebabkan oleh kelainan primer pada sumsum tulang dalam bentuk aplasia atau hipoplasia tanpa adanya infiltrasi, supresi atau pendesakan sumsum tulang. Pada anemia aplastik terjadi penurunan produksi sel darah dari sumsum tulang sehingga menyebabkan retikulositopenia, anemia, granulositopenia, monositopenia dan trombositopenia. Anemia aplastika termasuk dalam penyakit yang rentan disebabkan oleh faktor genetik. Salah satu faktor genetik yang banyak diidentifikasi adalah variasi gen atau single nucleotide polymorphism ( SNP). Hingga hari inimetodologi untuk bantuan variasi gen tersebut sudah tersedia dengan bentuk berbagai macam database dan bioinformatika. Tujuan penelitian ini untuk mengidentifikasi variasi gen yang berhubungan dengan Anemia aplastik dan memprioritaskan variasi gen tersebut berdasarkan tingkat kerentanannya melalui pemanfaatan katalog GWAS dan integrasi beberapa database bioinformatika. Hasil penelitian ini kami menemukan ada dua SNP rs1042151 dan rs28367832 yang rentan terhadap anemia aplastik berdasarkan ekpresi gen di jaringan darah . Variasi gen tesebut juga mengkode gen  HLA- DPB1 dan HLA-B dan menunjukkan ekspresi yang tinggi pada jaringan darah ( whole blood ) .Penelitian ini menunjukkan bahwa integrasi variasi gen dan bioinformatika potensial untuk memberikan informasi kepada si terkait kerentanan suatu variasi gen pada suatu penyakit termasuk pada anemia aplastik.
MISSENSE MUTATIONS IN THE IRAK1 GENE ARE ASSOCIATED WITH AN INCREASED RISK OF SYSTEMIC LUPUS ERYTHEMATOSUS Adnan Adnan; Dyah Aryani Perwitasari; Rita Maliza; Nanik Sulistyani
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.1073

Abstract

Single nucleotide polymorphisms (SNPs) are the most common form of genetic variation in humans. Missense SNPs can change protein structure and function. This study aimed to determine missense SNPs of the IRAK1 gene that can affect the amino acid sequence and lead to changes in protein structure and function, as well as their relationship as a risk factor for SLE. In this in silico method, several bioinformatics tools have been used to identify missense SNPs, including their properties and impacts, as well as their interaction networks with proteins. The tools used were PolyPhenv2, SIFT, PhD-SNP, PROVEAN, SNAP, Panthers, I-Mutant 3.0, and GeneMania. Four missense SNPs, rs11465830, rs1059702, rs1059703, and 10127175, were obtained from the NCBI SNP database. The SIFT test results showed that all the SNPs were tolerant. In the test results obtained using PolyPhen, the four SNPs were benign. The results of the probe test indicated that the four SNPs were neutral. When tested with SNAP, one SNP was neutral, and three others had an impact. In the PhD-SNP test, all SNPs were neutral. In the panther test, all SNPs were benign. The I-mutant assay showed that the four SNPs could decrease protein stability. Tests with GeneMania have reported that most interactions between genes were between IRAK1 and MYD88, and physical interactions were the most dominant form of interaction. Conclusion. rs10127175, rs11465830, rs1059702, and rs1059703 are missense SNPs in IRAK1, which can disrupt protein stability and be a risk factor for SLE. Keywords: IRAK1, SNP, Missense, Systemic Lupus Erythematosus
Co-Authors . Mulyadi Abdi Wira Septama Abdul Rahman Wahid ACHMAD NURYADIN AKBAR Ade Irawan Ade Irawan Adnan, Adnan Akrom, Akrom Aldri Frinaldi Alfian Syarifuddin Alvionida, Fitra Andhika Septiawan Anisa Devi Kharisma Wibowo Anisaningrum Anisaningrum Anisaningrum, Anisaningrum Anisaningrum, Anisaningrum Aprilia Kusbandari Astryna, Syarifah Yanti Athifah Candra Dewi Ayu Lifia Nur Kartikasari Definingsih Yuliastuti Dhega Agung Wichaksono Dinda Anindya Sabillah Dyah Aryani Perwitasari Eka Kumalasari Eka Kumalasari Fauziyya, Riri Fitra Alvionida Ghina Adhila Henry Budiawan Prasetya Ibnatul Azizah Ichwan Ridwan Rais Iin Narwanti IIN NARWANTI Ken Zaim Arifin Kintoko Kintoko Kintoko, Kintoko Kusuma, Nyoman Rudi La Malihi Laela Hayu Nurani Lalu Muhammad Irham Leswara, Dianita Febrina Lilies Kusuma Wardhani Lilies Kusuma Wardhani Lola Angelita Lukman Hardia Lukman Mahdi M. Kuswandi Mahdi, Lukman Maliza, Rita Melati Aprilliana Ramadhani, Melati Aprilliana Meta Ayuni Mika Triza Misba Muhammad Fathurrachman Mantali Muhammad Syakbani Mulyadi, . Mulyadi, . Mutti, Annisa Aulia Nina Salamah Nining Sugihartini NUR HASANAH Nurhayati Nurhayati Nurkhasanah Mahfudh Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Panji Ratih Suci Rahma Dona Rahmat Dani Satria Riat El Khair Ricke Desyratnaputri Rizky H. Mawardi Rofidah Nur Umar Sabrina Wulandari Sismindari . Sudjadi . Sun, Suny Tegar Prasetya Budi Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Wardhani, Lilies Kusuma Warsi Warsi Wirawan Adikusuma Wulandari, Sabrina Wulandari, Sabrina Zainab Zainab Zainab Zainab Zainab, Zainab