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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Disease Prevention and Public Health Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Jurnal Teknologi Laboratorium Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis Journal of Public Health and Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Tamilis Synex: Multidimensional Collaboration
Nanik Sulistyani
Fakultas Farmasi, Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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PENETAPAN PARAMETER STANDARDISASI NON SPESIFIK DAN SPESIFIK EKSTRAK DAUN PACAR KUKU (Lawsonia inermis L.) Zainab Zainab; Nanik Sulistyani; Anisaningrum Anisaningrum
Media Farmasi: Jurnal Ilmu Farmasi Vol 13, No 2: September 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.267 KB) | DOI: 10.12928/mf.v13i2.7773

Abstract

Standardisasi ekstrak tumbuhan obat di Indonesia merupakan salah satu tahapan penting dalam pengembangan obat asli Indonesia. Daun pacar kuku (Lawsonia inermis L.) merupakan salah satu tanaman yang mengandung senyawa naftokuinon dengan berbagai aktivitas. Penelitian ini bertujuan untuk melakukan standardisasi ekstrak daun pacar kuku sehingga diharapkan dapat digunakan sebagai acuan parameter standar mutu ekstrak karena standarisasi ekstrak pacar kuku belum tercantum di MMI dan FHI. Dalam penelitian ini penyarian ekstrak daun pacar kuku dilakukan menggunakan metode infundasi. Penetapan parameter non spesifik meliputi kadar air, kadar abu total, kadar abu tidak larut asam dan cemaran mikroba dengan metode ALT. Penetapan parameter spesifik yaitu dengan mengetahui organoleptis ekstrak, profil KLT dan menetapkan kadar naftokuinon total menggunakan metode kromatografi lapis Tipis (KLT) dengan fase diam silika gel 60 F254, fase gerak metanol : kloroform (2:8) dan analisis kuantitatif menggunakan densitometri pada panjang gelombang  maksimal. Hasil standardisasi untuk parameter non spesifik menunjukkan kadar air (7,33±0,52% v/b), kadar abu total (6,43±0,25%), kadar abu tidak larut asam (0,106±0,004%), dan uji cemaran mikroba (85x102 CFU/g). Hasil untuk parameter spesifik menunjukkan organoleptis ekstrak (kental, warna coklat tua, rasa agak pahit dan berbau khas daun pacar kuku), dengan kandungan senyawa naftokuinon, kumarin, flavonoid, polifenol, alkaloid, dan kadar naftokuinon total (7,43±0,28%). Ekstrak daun pacar kuku sudah memenuhi persyaratan sesuai acuan standar Farmakope Herbal Indonesia tentang syarat ekstrak sebagai bahan baku sediaan obat tradisional.
PENGARUH MEDIA TERHADAP PERTUMBUHAN ISOLAT ACTINOMYCETES KODE AL35 SERTA OPTIMASI PRODUKSI METABOLIT ANTIBAKTERI BERDASARKAN WAKTU FERMENTASI DAN pH Sabrina Wulandari; Nanik Sulistyani
Media Farmasi: Jurnal Ilmu Farmasi Vol 13, No 2: September 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.342 KB) | DOI: 10.12928/mf.v13i2.7770

Abstract

Actinomycetes merupakan salah satu mikroorganisme yang dapat menghasilkan metabolit sekunder antara lain antibiotik. Isolat Actinomycetes (AL35) telah diisolasi dari rizosfer tanaman padi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh media terhadap pertumbuhan isolat AL35 serta optimasi produksi metabolit antibakteri berdasarkan waktu fermentasi dan pH. Media pertumbuhan yang digunakan adalah ISP 1, ISP 2, ISP 3, ISP 4, ISP 5 dan media SNA. Pertumbuhan isolat diamati berdasarkan miselium udara dan miselium vegetatif. Optimasi waktu produksi metabolit antibakteri dilakukan dengan waktu fermentasi  hari ke 1 hingga hari ke 14. Optimasi dilakukan pada pH 5, 6, 7, 8, 9 dan 10 dengan waktu inkubasi 14 hari. Metode uji aktivitas antibakteri  adalah metode sumuran.  Parameter yang diukur adalah diameter zona hambat terhadap Staphylococcus aureus dan Eschericia coli di sekitar sumuran. Profil optimasi  produksi metabolit sekunder berdasarkan pH dianalisis dengan membuat grafik hubungan antara pH dan diameter zona hambat, sedangkan berdasarkan waktu fermentasi, dibuat grafik hubungan antara waktu fermentasi dan zona hambat. Hasil penelitian menunjukkan bahwa isolat AL35 dapat tumbuh pada semua media yang digunakan dengan tingkat kesuburan yang berbeda-beda. Cairan kultur isolat AL35 dapat menghambat pertumbuhan S. aureus dan E. coli. Media yang menghasilkan tingkat kesuburan pertumbuhan isolat AL35 berturut-turut dari yang paling subur adalah media ISP4 lalu ISP5, SNA, ISP2, ISP3, dan ISP1. Waktu fermentasi untuk produksi metabolit antibakteri adalah hari ke 2, sedangkan pH optimal adalah pH 9 berdasarkan aktivitas terhadap S. aureus dan pH 6 terhadap E. coli.
Genetic Characteristics of Actinomycetes Isolates (GST, KP, KP11, Kp16, T24, T37 Code) by RFLP of NRPS Genes Melati Aprilliana Ramadhani; Nanik Sulistyani
Media Farmasi: Jurnal Ilmu Farmasi Vol 12, No 1: Maret 2015
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.693 KB) | DOI: 10.12928/mf.v12i1.3020

Abstract

Actinomycetes is the one of antibiotic producing microorganisms. Actinomycetes isolates with GST, KP, KP11, KP16, T24, T37 have been isolated from soil. This study aims to determine the diversity of actinomycetes isolates by RFLP (Restriction Fragment Length Polymorphism) of NRPS (Non Ribosomal Peptide Synthetase) genes from GST, KP, KP11, KP16, T24, and T37 isolates. This study is divided into several steps of the DNA isolation  from isolate GST, KP16, T24, T37, KP, KP11, PCR of the 16S rRNA and NRPS genes and cutting the NRPS genes fragmens with HaeIII enzyme to determine the diversity of isolates. Results of DNA isolate, PCR of 16S rRNA genes and NRPS genes, and RFLP of NRPS genes performed by agarose gel electrophoresis. The diversity of the isolates were analyzed by multivariate analysis UPGMA with MVSP 2.0 applications. The results showed the diversity of 6 actinomycetes isolates are T37 and GST have 64 % similarity, KP11 and KP16 have 96 % similarity, KP has 76 % similarity with KP11 and KP16, while T24 has 60 % similarity with KP and 12 % similarity with T37 and GST. Based on RFLP analysis of the NRPS genes, among of 6 isolates can divided into 4 groups are T37 and GST isolates as group 1, KP11 and KP16 isolates as group 2, KP isolate as group 3, and T24 isolate as group 4.
Antibacterial Compound Identification of Cayenne Pepper Leaf Extract (Capsicum frutescens L.) against Klebsiella pneumoniae and Cell Leakage Mechanism Riri Fauziyya; Laela Hayu Nurani; Nanik Sulistyani
Majalah Obat Tradisional Vol 22, No 3 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1037.003 KB) | DOI: 10.22146/mot.31550

Abstract

Pneumonia is an acute inflammation of the pulmonary parenchyma that can be caused by Klebsiella pneumoniae. This study aims to determine the active fraction of cayenne pepper leaves on the growth of K. pneumoniae. Cayenne pepper leaf which previously defatted using n-hexane was macerated with 95% ethanol, then fractionated successively with dichloromethane, ethyl acetate and methanol. Ethanol extract and each fraction with concentration of 40% were tested for their antibacterial activity against K. pneumoniae using disc diffusion method (Kirby-Bauer). 1% amoxicillin was used as positive control and Dimethyl sulfoxide (DMSO) as negative control. The Minimum Inhibitory Concentration (MIC) of the most active fraction was then determined. Determination of antibacterial compound in the most active fraction was carried out by TLC-bioautography and followed by Gass Chromatography Mass Spectrophotometry. Cell leakage analysis was performed using UV spectrophotometry to detect the release of protein and nucleic acid, as well as Atomic Absorption Spectrophotometry was used to detect ion release of K+ and Ca2+. The results showed that the most active fraction against K. pneumoniae was the ethyl acetate fraction with MIC value of 10% and inhibition zone of 7.25±0.25 mm. TLC-Bioautography of ethyl acetate fraction with eluen n-hexane: ethyl acetate (6:4) obtained an active stain at Rf 0.12. Compounds having 94% similarity with 1-propanol, 2-amino was predicted as the active compound.
The antiviral activity of srikaya seed (Annona squamosa L.) ethanolic extract against newcastle disease virus in chicken embryo Nanik Sulistyani; Ibnatul Azizah; M. Kuswandi
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.358 KB) | DOI: 10.14499/indonesianjpharm0iss0pp62-67

Abstract

Srikaya (A. squamosa L.) is a plant containing polyphenol, flavonoid, tannin, alkaloid and saponin compounds. These compounds could be extracted by ethanol solvent. Previous study shown that infuse of Srikaya seed had antiviral activity with value of IC50 3,236 'g/mL against Newcastle Disease Virus (NDV). Therefore, the aim of research is to know the potency of ethanolic extract of Srikaya seed as antiviral against Newcastle Disease Virus.The 28 chicken embryos at age of 9-11 days were classified into 7 groups, i.e. : control of virus, control of solvent ethanol : aquadest (1:10) and treatment groups were given ethanolic extract of Srikaya seed with concentration 0.1 'g/mL; 0.15 'g/mL; 0.2 'g/mL; 0.25 'g/mL; dan 0.3 'g/mL. NDV was inoculated into allantoic cavity intermediate after inoculated of extract and then incubated for 2 days. Antiviral activity were observed by hemaglutination method. The value of IC50 was calculated by probit analysis.The result showed that ethanol extract of srikaya seed (A. squamosa L.) had antiviral activity because of increasing concentration caused the higher inhibition percentage of hemaglutination. The value of IC50 is 0.152 'g/mL.Key words: Antiviral, Annona squamosa, Newcastle Disease Virus (NDV).
THE CLEAVING ACTIVITY ASSAY ON SUPERCOILED DNA BY PROTEIN FRACTIONS FROM Morinda citrifolia LEAVES Nanik Sulistyani; Sismindari .; Sudjadi .
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.316 KB) | DOI: 10.14499/indonesianjpharm0iss0pp174-179

Abstract

Study on supercoiled DNA cleaving activity assay of protein fraction from Morinda citrifolia leaf had been done. The aim of this research is to compare the supercoiled DNA cleaving abilities among the fractions. This is a preliminary screening to find RIP from Morinda citrifolia Fractionation of protein was done by adding Ammonium Sulphate up to 20%, 40%, 60%, 80% and 100% in the crude extract, saturated solution was then centifugated to get the pellet fractions and coded as F-20, F-40, F-60, F-80 and F-100. The mixture of pUC19 plasmid and protein fractions, in the same protein concentration were incubated at room temperature for one hour, the cleaving ability of protein fraction on supercoiled DNA.was investigated. The results indicated that protein fractions, of F-80 had the highest cleaving activity to supercoiled DNA.Key words : Ribosome-Inactivating Protein (RIP), supercoiled DNA , protein fraction, Morinda citrifolia.
The antiviral activity of srikaya seed (Annona squamosa L.) ethanolic extract against newcastle disease virus in chicken embryo Nanik Sulistyani
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp62-67

Abstract

Srikaya (A. squamosa L.) is a plant containing polyphenol, flavonoid, tannin, alkaloid and saponin compounds. These compounds could be extracted by ethanol solvent. Previous study shown that infuse of Srikaya seed had antiviral activity with value of IC50 3,236 'g/mL against Newcastle Disease Virus (NDV). Therefore, the aim of research is to know the potency of ethanolic extract of Srikaya seed as antiviral against Newcastle Disease Virus.The 28 chicken embryos at age of 9-11 days were classified into 7 groups, i.e. : control of virus, control of solvent ethanol : aquadest (1:10) and treatment groups were given ethanolic extract of Srikaya seed with concentration 0.1 'g/mL; 0.15 'g/mL; 0.2 'g/mL; 0.25 'g/mL; dan 0.3 'g/mL. NDV was inoculated into allantoic cavity intermediate after inoculated of extract and thenincubated for 2 days. Antiviral activity were observed by hemaglutination method. The value of IC50 was calculated by probit analysis.The result showed that ethanol extract of srikaya seed (A. squamosa L.) had antiviral activity because of increasing concentration caused the higher inhibition percentage of hemaglutination. The value of IC50 is 0.152 'g/mL.Key words: Antiviral, Annona squamosa, Newcastle Disease Virus (NDV).
Inhibitory Activity of Parsea americana Mill. Peels Extract and Fraction Containing Phenolic Compound Against Staphylococcus aureus ATCC 25923 Nanik Sulistyani; Lola Angelita; Nurkhasanah Nurkhasanah
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 19, No 1 (2022)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.784 KB) | DOI: 10.24071/jpsc.003005

Abstract

Parsea americana Mill. is a natural resource that has been studied for its antibacterial properties. The pulp, peel, and seed of Parsea americana Mill. have potential as an antibacterial agent. This study aimed to determine the inhibitory activity and phenolic content of Parsea americana Mill. peels extract and fraction against Staphylococcus aureus ATCC 25923. Parsea americana Mill. was macerated with 96% ethanol and then fractionated with n-hexane, ethyl acetate, and methanol solvent. Determination of the fraction that has the greatest antibacterial activity against S. aureus was carried out using the Kirby Bauer method.  The total phenolic content in the extract and fraction was calculated as gallic acid equivalent (GAE) using the Folin-Ciocalteu method spectrometrically. Antibacterial activity test of the 96% ethanol extract, ethyl acetate fractions, and methanol fractions at a concentration of 10% w/v showed activity with a measurable inhibition zone. On the other hand, the n-hexane fraction showed no inhibition zone. The highest inhibition zone was the ethyl acetate fraction with approximately 8.33 ± 0.58 mm. The ethyl acetate fraction of Parsea americana Mill. resulted in 536.26 ± 14.29 mg GAE/g fraction. The conclusion was that the ethyl acetate fraction had the highest total phenolic content and was the most active fraction in inhibiting the growth of Staphylococcus aureus.
PELATIHAN PERAN SERTA KADER POSYANDU DALAM PEMBERIAN EDUKASI KEPADA MASYARAKAT Nina Salamah; Nanik Sulistyani
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 2 No. 2 (2018)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v2i2.393

Abstract

Posyandu merupakan wadah yang dapat digunakan untuk upaya pencegahan dan penanggulangan masalah kesehatan baik gizi, kesehatan ibu dan anak, Pola hidup bersih sehat, imunisasi dan sebagainya.  Lewat model penimbangan dan pemantauan gizi tiap bulan juga dapat dijadikan sebagai alat surveilans gizi yang tujuan akhirnya adalah upaya pencegahan dan penanggulangan masalah gizi balita. Jika pelaksanaan Posyandu baik maka target pragram akan terpenuhi, akan tetapi jika pelaksanaan posyandu kurang lancar maka target tidak akan bisa terpenuhi. Dalam praktek di masyarakat pelaksanaan Posyandu masih mengalami beberapa kendala antara lain kemampuan kader yang masih kurang dalam kegiatan penyuluhan kesehatan kepada masyarakat. Tujuan pengabdian ini yaitu untuk meningkatkan kemampuan kader posyandu dalam pengukuran dan penimbangan balita serta status gizi ibu dan balita. Perlu juga peningkatan kemampuan penyuluhan kader, mengingat keterbatasan tenaga penyuluh di puskesmas Depok 3. Hal ini berimplikasi pada penanganan permasalahan kesehatan ibu dan balita lebih cepat. Metode yang digunakan dalam pelatihan ini yaitu dengan pemberian kegiatan pelatihan-pelatihan untuk peningkatan skill kader Posyandu disertai dengan mengadakan lomba penyuluhan kepada masyarakat dan  pemberian hadiah berupa ung pembinaan untuk melengkapi melengkapi sarana pra sarana yang menunjang kegiatan Posyandu. Melalui kegiatan ini diharapkan peran Posyandu sebagai ujung tombak pemantauan status gizi di masyarakat dapat lebih optimal. Hasil dari kegiatan ini meningkatkan kemampuan kader dalam memberikan penyuluhan kepada masyarakat dengan metode ang lebih menarik sehingga meningkatkan kepercayaan diri kader untuk bisa berbagi kepada masyarakat tentang kesehatan. Pelatihan ini meluas karena juga diberikan kepada kader posyandu lansia dan juga posbindu.
KARAKTERISASI FRAKSI TERAKTIF SENYAWA ANTIBIOTIK ISOLAT KP 13 DENGAN METODE DENSITOMETRI DAN KLT-SEMPROT Alfian Syarifuddin; Nanik Sulistyani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 4 No 1 (2019): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.99 KB) | DOI: 10.36387/jiis.v4i1.263

Abstract

Exploration of microorganisms Actinomycetes can produce antibiotic compounds. One of the bacteria that can produce antibiotic compounds is the KP13 bacterial isolate. The purpose of this study was to determine the character of the fraction of KP13 isolates using the densitometry method and the spray / colouring reaction of thin layer chromatography elution. The wavelengths used for densitometry 210 nm and spray reagents used include ninhydrin, Dragendorff, 2,4-dinitrophenyl hydrazine (2,4-DNPH), and sulfuric acid anisaldehyde. The results of the study using densitometry at a wavelength of 210 nm showed the highest peak chromatogram at Rf 0.78. The compound that is thought to be responsible for the Rf value of 0.78 has the characteristics of Terpenoid compounds, alkaloids, and carbonyl.
Co-Authors . Mulyadi Abdi Wira Septama Abdul Rahman Wahid ACHMAD NURYADIN AKBAR Ade Irawan Ade Irawan Adnan, Adnan Akrom, Akrom Aldri Frinaldi Alfian Syarifuddin Alvionida, Fitra Andhika Septiawan Anisa Devi Kharisma Wibowo Anisaningrum Anisaningrum Anisaningrum, Anisaningrum Anisaningrum, Anisaningrum Aprilia Kusbandari Astryna, Syarifah Yanti Athifah Candra Dewi Ayu Lifia Nur Kartikasari Definingsih Yuliastuti Dhega Agung Wichaksono Dinda Anindya Sabillah Dyah Aryani Perwitasari Eka Kumalasari Eka Kumalasari Fauziyya, Riri Fitra Alvionida Ghina Adhila Henry Budiawan Prasetya Ibnatul Azizah Ichwan Ridwan Rais Iin Narwanti IIN NARWANTI Ken Zaim Arifin Kintoko Kintoko Kintoko, Kintoko Kusuma, Nyoman Rudi La Malihi Laela Hayu Nurani Lalu Muhammad Irham Leswara, Dianita Febrina Lilies Kusuma Wardhani Lilies Kusuma Wardhani Lola Angelita Lukman Hardia Lukman Mahdi M. Kuswandi Mahdi, Lukman Maliza, Rita Melati Aprilliana Ramadhani, Melati Aprilliana Meta Ayuni Mika Triza Misba Muhammad Fathurrachman Mantali Muhammad Syakbani Mulyadi, . Mulyadi, . Mutti, Annisa Aulia Nina Salamah Nining Sugihartini NUR HASANAH Nurhayati Nurhayati Nurkhasanah Mahfudh Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Panji Ratih Suci Rahma Dona Rahmat Dani Satria Riat El Khair Ricke Desyratnaputri Rizky H. Mawardi Rofidah Nur Umar Sabrina Wulandari Sismindari . Sudjadi . Sun, Suny Tegar Prasetya Budi Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Wardhani, Lilies Kusuma Warsi Warsi Wirawan Adikusuma Wulandari, Sabrina Wulandari, Sabrina Zainab Zainab Zainab Zainab Zainab, Zainab