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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Disease Prevention and Public Health Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Jurnal Teknologi Laboratorium Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis Journal of Public Health and Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Tamilis Synex: Multidimensional Collaboration

Nanik Sulistyani

Fakultas Farmasi, Universitas Ahmad Dahlan

Author-ID : 391304

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Chloroform fraction of ethanolic extract of Elephantopus scaber Linn. increase the p53 expression on human breast cancer (T47D) cell line Nurkhasanah Mahfudh; Nanik Sulistyani; Lukman Mahdi
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Elephantopus scaber Linn had been used as traditional medicine. It contain some lacton sesquiterpene with antiproliferative activity. The study aim was to explore the effect of chloroform fraction of ethanolic extract of E.scaber on the expression of p53 on T47D cell line. The ethanol extract was found by maceration method. The extract was then fractionated using petroleum ether and chloroform respectively to get the chloroform fraction. The expression of p53 was observed by immunocytochemistry method with antip53 antibody. The p53 cell line was cultured and treated with fraction with concentration of 7.06 and 3.53 Âµg/ml. The expression of p53 was showed by brown colour in the nucleus of the cell. The result showed that chloroform fraction treatment with concentration of 7.06 Âµg/ml induced apoptotic cell death in the end stage (necrosis). While the treatment with lower concentration 3.53 Âµg/ml could increase the p53 expression.

In vitro immunomodulatory activity test of Bengle rhizoma extract (Zingiber cassumunar Roxb.): phagocytic activity of macrophages and lymphocyte proliferation in mice Ghina Adhila; Nurkhasanah Nurkhasanah; Nanik Sulistyani
Pharmaciana Vol 9, No 2 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Immunomodulators are pharmacological agents that affect the immune system at different levels. Aside from modulating, some immunomodulators stimulate, while some others inhibit immune responses. Zingiber cassumunar Roxb. or Bengle rhizoma has been reported to exhibit immunomodulatory activities. This research was intended to determine the pharmacological effects of its extract on the phagocytic activity Â of Â macrophages and lymphocyte proliferation in vitro. It used the macrophages and lymphocytes of male BALB/c mice, which were divided into normal control and treatment group (receiving 25, 50, and 100 ppm of extract). The immunomodulatory activity test results showed that 100 ppm of Bengle rhizoma extract reduced phagocytosis in macrophages much significantly than the control group and that the treatment groups suppressed the proliferation of lymphocytes more Â substantially than the control group. The extract decreased the phagocytic activity of macrophages and the proliferation capacity of lymphocytes when administered at a concentration of 100 ppm.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN BINAHONG (Anredera scandens (L.) Moq.) TERHADAP Shigella flexneri BESERTA PROFIL KROMATOGRAFI LAPIS TIPIS Lilies Kusuma Wardhani; Nanik Sulistyani
Pharmaciana Vol 2, No 1 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Antibacterial compounds that can be used for the treatment of infections causedby bacteria. Traditionally, Anredera scandens is one of plant used as traditionalmedicine. The aims of this research are to find out the antibacterial activity of ethylacetate extract of Anredera scandens leaf and Minimum Bactericidal Concentration(MBC) against Shigella flexneri and to identify the chemical subtances of the ethylacetate extract found in Anredera scandens. The Anredera scandens leaf was extractedby use of maseration method using ethyl acetate as solvent. The antibacterial assay wasdone using liquid dilution method with varied concentration of Anredera scandens(8,5%, 8%, 7,5%, 7%, 6,5%, 6%, 5,5%, dan 5% w/v) to determine MBC.Chromatography test was done to identify the chemical substances of Anrederascandens extract. The result of this research showed that the MBC of Anrederascandens leaf extract was 8% w/v. The result of the phytochemical screening with tubetest and thin layer chromatogram showed that the extract of Anredera scandens leafcontained, polyphenols, and saponin.

Leakage Induced in Eschericia coli Cells by Secondary Metabolites of the J7 Bacterial Isolates from the Rhizosphere of Zingiber officinale Roscoe var. Rubrum Dianita Febrina Leswara; Nanik Sulistyani; Kintoko Kintoko
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Isolate J7 is a bacterial strain separated from the rhizosphere of Zingiber officinale Roscoe var. Rubrum that potentially produces antibacterial compounds against Escherichia coli. The study aimed to determine the antibacterial activity of the most active fraction of this isolate from its capacity to induce leakage in E. coli cells. The secondary metabolites were extracted from Isolate J7 using ethyl acetate solvent and then fractionated with different ratios of hexane and ethyl acetate solventsâ€•1:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, and 0:1 (v/v), ethyl acetate and methanol with 1:1 (v/v) ratio, and methanol 100%. Fractions were identified based on the spotting on the Thin-Layer Chromatography (TLC) plate. Only the most active fraction was tested to define its ability to cause leakage of cellular components like nucleic acid and protein. The leakage was scanned with a UV-Vis spectrophotometer at wavelengths of 260 and 280 nm. The results showed that F2 was the fraction that prevented the growth of E. coli most actively because it created a zone of inhibition sized 11.58Â±0.95 mm in diameter with the lowest MIC among the other fractions (10%). Also, based on the spectrophotometric analysis, the addition of F2 at concentrations of 10% and 20% resulted in higher levels of protein, DNA, and RNA than the negative control. In conclusion, F2 can cause plasma membrane leakage in E. coli at a concentration of 10%. Another fraction that exhibited antibacterial activity was F3. In the spotting analysis of the TLC plate, F3 appeared to have a spot profile and Rf that were similar to F2 but considerably different from the inactive fractions (i.e., showing no antibacterial activity). Observed with multiple wavelengths, the Rf values of F2 and F3 spots varied between 0.56-0.57 and 0.61-0.62 (254 nm) and 0.47-0.48 and 0.56-0.57 (366 nm), respectively. Because these spot profiles did not appear in the inactive fractions, compounds with this range of Rf values are, thereby, suspected as the active substances that inhibit the growth of E. coli.

AKTIVITAS ANTIFUNGI EKSTRAK ETANOL BATANG BINAHONG (Anredera cordifolia (Tenore) Steen.) TERHADAP Candida albicans SERTA SKRINING FITOKIMIA Eka Kumalasari; Nanik Sulistyani
Pharmaciana Vol 1, No 2 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Anredera cordifolia is a plantused as antifungal empirically. This study aims to find out the antifungal activity of ethanol extract of A. cordifolia stem against C. albicans and to identify the chemical subtance groups of the ethanol extract of A. cordifolia. The A. cordifolia stem was extracted by maceration method using ethanol 70 % as solvent. The antifungal assay was done using liquid dilution method with various concentrations of (85; 86; 87; 88; 89; 90% w /v). The mixtures of extract and suspension of C. albicans in CYG (Casein Yeast Glucose) medium and the media were incubated at 37ºC for 18-24 hours to determine Minimum Inhibitor Conceration (MIC). The culture was streaked on SDA (Sabouraud Dextrose Agar) medium to determine Minimum Fungicidal Conceration (MFC). Chromatographic test and tube test were done to identify the chemical substances of A. cordifolia extract. The result showed that the MIC could not be determined because the extract was brownish and turbid while the MFC of A. cordifolia stem ethanolic extract was 86% w /v. The result of the phytochemical screening with tube test and the thin layer chromatography test showed that the extract of A. cordifolia stem contained flavonoids, polyphenols, and saponin.

KERAGAMAN ISOLAT ACTINOMYCETES BERDASARKAN ANALISIS RFLP TERHADAP GEN NRPS Nanik Sulistyani
Pharmaciana Vol 3, No 1 (2013): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Actinomycetes is one of the types of microorganisms that is essential forproducing the active compounds, one of which is an antibiotic. Isolation ofActinomycetes from the rhizosphere of rice plant (Oryza sativa L.) and tin plant (Ficuscarica L.) has yielded 13 isolates of antibiotic-producing Actinomycetes. This studyaims to determine the diversity of isolates by RFLP analysis (Restriction FragmentLength Polymorphism) of the NRPS (Non Ribosomal Peptide Synthetase) gene. Thestudy was carried out by isolating Actinomycetes isolate DNA and performed PCR onthe 16SrRNA and NRPS genes. The RFLP analysis of the NRPS gene PCR products isthen performed using the enzyme HaeIII. Identify the presence of DNA, the PCRproduct and RFLP results was performed by agarose gel electrophoresis. The diversityof isolates was analyzed by multivariate analysis. The results showed that the diversityof 13 isolates of Actinomycetes can be grouped into 5 groups: isolates 5 (group 1),isolates 8 (group 2), isolates 11 (group 3), isolates 6,7,9 (group 4) and isolates1,2,3,4,10,12,13 (group 5).

The effect of Zingiber cassumunar Roxb rhizome extract on in vitro phagocytic activity and lymphocyte proliferation Nurkhasanah Mahfudh; Nanik Sulistyani; Dinda Anindya Sabillah
Pharmaciana Vol 10, No 2 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Zingiber cassumunar Roxb (bengle) is one of the plants with the potential as an immunomodulator. It contain curcumin and some volatile compounds which previously reported to be potential as immunomodulator. This study aims to examine and determine the effect of ethyl acetate fraction of bengle extract on phagocytic activity of macrophages and lymphocyte proliferation in vitro. The bengle rhizome is macerated with 96% etanol solvent and then followed by ethyl acetate fractionation. The macrophage and lymphocyte were used in this study was isolated from male Balb/C strain mice. The macrophage phagocytosis activity was carried out using latex bead assay, and lymphocyte proliferation assay was carried out using MTT assay method. The concentration of extract was used in this study were 25, 50 and 100 Î¼g/mL. The effect of treatment was compared to negative control group. Â The results showed that ethyl acetate fraction of bengle extract can increase the phagocytic activity of macrophages was shown by increasing of active phagocytes cells (SFA) and phagocytosis index. The highest percentage of SFA and phagocytic index results were shown at a concentration of 100 Î¼g/mL. The results showed that ethyl acetate fraction of bengle extract could increase lymphocyte proliferation significantly compare to the negative control (p<0.05). This study showed the potential of bengle as immunomodulatory agent.

Active antimicrobial substances of cherry leaf extracts (Muntingia calabura L.) against Methicillin-Resistant Staphylococcus aureus (MRSA) based on GC-MS analysis Nanik Sulistyani; Mika Triza Misba; Nurkhasanah Nurkhasanah
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the bacteria that triggers nosocomial diseases. Bacterial resistance requires continuous exploration of active antimicrobial substances from various sources, including medicinal plants. Leaves of cherry (Muntingia calabura L.) reportedly contain three classes of compounds, namely, tannins, flavonoids, and saponins. This research was designed to identify active antimicrobial substances in cherry leaves that could inhibit the growth of MRSA. It employed the Kirby-Bauer test to examine the antimicrobial activities of the leaf extracts of Muntingia calabura L. (EMC) against MRSA. Through GC-MS, active substances were detected from the presence of active spots on TLC plates, as determined by direct-contact bioautography. The TLC used silica gel F254 as the stationary phase and chloroform:ethyl acetate (9:1) as the mobile phase. The antimicrobial activity test results showed that the zone of inhibition of 10% w/v EMC was 10.91Â±0.75 mm in diameter. At 5% w/v and 2.5% w/v, EMC created zones of inhibition with diameters of 8.5Â±0.25 mm and 7.25Â±0.25 mm, respectively. Meanwhile, at 1.25% w/v, it showed no inhibitory activities. Based on the TLC-Bioautography profile, the active spot that produced zones of inhibition was located at Rf 0.04 mm. The GC-MS analysis of this spot detected the presence of two compounds: the first compound had a similarity index of 35% with 3,11,13-triacetycynaratriol, and the second one had a similarity index of 80% with hexaborane-12. Cynaratriol is known to posses antimicrobial activity, whereas hexaborane is the opposite. In conclusion, the minimum inhibitory concentration of EMC for MRSA is 2.5% w/v. Also, the active compounds of EMC bear 35% similarities to 3,11,13-triacetycynaratriol.

Uji sitotoksisitas dan antiproliferatif ekstrak etanol daun leunca (Solanum Nigrum,L) terhadap sel raji Rahma Dona; Nanik Sulistyani; Laela Hayu Nurani
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Leunca (Solanum nigrum,L.) is one of the medicinal plant which used society for traditional medication as antipyretic, hypotensive and anticancer. The aim ofÂ this research was to know an anticancer activity of ethanol extract of leunca leaves, emphasized on an ability to inhibit the growth Raji cell. Ethanol extract took from leunca leaf powder that epitomized using ethanol solvent by Soxhlet instrument. Citotoxicity test was done by incubating Raji cell at a density of 2x104 with treatment using ethanol extract from leunca (Solanum nigrum L.) leaf in several concentration 500; 250; 125; 62,5; 31,25; 15,62; 7,81 and 3,90 Âµg/ml. A test was done by MTT method and the percent of cell mortality was calculated. The LC50 values were calculated using probit analysis. The research continued with antiproliferation test on treatment sample concentration 50; 25 Âµg/ml with cell control for 24, 48, and 72 hours.The result of research indicate that ethanol extract of leunca leaves had cytotoxic effect towards Raji cell with LC50 values 59,22 Âµg/ml. The result ofÂ antiproliferation test showed that there were the growth of inhibitation on treatment sample with doubling time values 69,56 hour at concentration 50 Âµg/ml; 60,00 hour at concentration 25 Âµg/ml, and doubling time cell control is 44,98 hour.

Composition of carbopol 940 and HPMC affects antibacterial activity of beluntas (Pluchea indica (L.)) leaves extract gel Fitra Alvionida; Nanik Sulistyani; Nining Sugihartini
Pharmaciana Vol 11, No 3 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Indonesia is a country known for its source of biological wealth, one of which is beluntas leaves. Beluntas leaves have the potential to be an antibacterial, so it is appropriate to be formulated in the form of medicinal preparations, especially gels. This study aims to find out the influence of variations between carbopol gel base 940 and Methylcellulose Hydroxypropyl (HPMC) on the physical properties of gel preparations beluntas leaf extract (Pluchea indica (L.), and know the influence of gel of extract of beluntas leaves on antibacterial activity. The extract is obtained by the maceration method using ethanol solvent 96%. Each formula uses 15% of the extract of beluntas leaves. Gels are made in four gel base variations namely F0 (0.5% carbopol, 1% HPMC), FI (1% carbopol, 1.5% HPMC), FII (1.5% carbopol, 2.5% HPMC), and FIII (2% carbopol, 3% HPMC). Gels evaluated for their physical properties include organoleptic, viscosity, pH, homogeneity, scattering power, adhesion and Freeze-thaw cycling. Then the gel tested antibacterial activity against bacteria Staphylococcus aureus and Pseudomonas aeruginosa by cup-plate diffusion method. The data obtained were analyzed with One Way Anova and LSD with a 95% confidence level. The results showed that beluntas leaf extract gel meets the organoleptic requirements, homogeneity, good gel adhesion (> 4sec), good gel viscosity (2000-50.000 cps), and good gel pH (4.5-6.5). However, the gel does not meet the requirements of good scattering power (5-7 cm) and Freeze-thaw cycling. Based on the test results that have been done with some of the parameters above, The best composition of carbopol 940 and HPMC in the beluntas leaf extract gel which has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa Â is 1% carbopol and 1.5% HPMC. The antibacterial activity of the formula is categorized as strong.

Co-Authors . Mulyadi Abdi Wira Septama Abdul Rahman Wahid ACHMAD NURYADIN AKBAR Ade Irawan Ade Irawan Adnan, Adnan Akrom, Akrom Aldri Frinaldi Alfian Syarifuddin Alvionida, Fitra Andhika Septiawan Anisa Devi Kharisma Wibowo Anisaningrum Anisaningrum Anisaningrum, Anisaningrum Anisaningrum, Anisaningrum Aprilia Kusbandari Astryna, Syarifah Yanti Athifah Candra Dewi Ayu Lifia Nur Kartikasari Definingsih Yuliastuti Dhega Agung Wichaksono Dinda Anindya Sabillah Dyah Aryani Perwitasari Eka Kumalasari Eka Kumalasari Fauziyya, Riri Fitra Alvionida Ghina Adhila Henry Budiawan Prasetya Ibnatul Azizah Ichwan Ridwan Rais Iin Narwanti IIN NARWANTI Ken Zaim Arifin Kintoko Kintoko Kintoko, Kintoko Kusuma, Nyoman Rudi La Malihi Laela Hayu Nurani Lalu Muhammad Irham Leswara, Dianita Febrina Lilies Kusuma Wardhani Lilies Kusuma Wardhani Lola Angelita Lukman Hardia Lukman Mahdi M. Kuswandi Mahdi, Lukman Maliza, Rita Melati Aprilliana Ramadhani, Melati Aprilliana Meta Ayuni Mika Triza Misba Muhammad Fathurrachman Mantali Muhammad Syakbani Mulyadi, . Mulyadi, . Mutti, Annisa Aulia Nina Salamah Nining Sugihartini NUR HASANAH Nurhayati Nurhayati Nurkhasanah Mahfudh Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Panji Ratih Suci Rahma Dona Rahmat Dani Satria Riat El Khair Ricke Desyratnaputri Rizky H. Mawardi Rofidah Nur Umar Sabrina Wulandari Sismindari . Sudjadi . Sun, Suny Tegar Prasetya Budi Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Wardhani, Lilies Kusuma Warsi Warsi Wirawan Adikusuma Wulandari, Sabrina Wulandari, Sabrina Zainab Zainab Zainab Zainab Zainab, Zainab

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