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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ZOO INDONESIA Jurnal Matematika Sains dan Teknologi Jurnal Perikanan Universitas Gadjah Mada Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Zoo Indonesia Jurnal Airaha Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Kefarmasian Indonesia
Iman Supriatna
Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Efektivitas Insulin-Like Growth Factor-I (IGF-I) dalam Media Maturasi In Vitro Pada Pematangan Inti dan Fertilisasi Oosit Sapi Bali . Hasbi; Sri Gustina; Ni Wayan Kurniani Karja; Iman Supriatna; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.643 KB) | DOI: 10.29244/avi.6.1.24-29

Abstract

Penelitian ini bertujuan mengetahui efektivitas insulin-like growth factor–I (IGF-I) pada tingkat pematangan inti dan fertilisasi oosit sapi bali. Penelitian ini dibagi dalam dua tahap. Tahap I, oosit dimatangkan secara in vitro dalam media 199 dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I. Tahap II, oosit dimatangkan dalam media seperti pada penelitian tahap I, kemudian difertilisasi secara in vitro untuk mengamati pembentukan pronukleus. Hasil penelitian tahap I menunjukkan bahwa tidak terdapat perbedaan jumLah oosit yang mencapai tahap metaphase II (MII) dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I dalam media maturasi. Berturut-turut adalah 80,6±7,6%; 81,5±8,6%; 87,5±6,9%; dan 84,1±12,4%. Penelitian tahap II menunjukkan bahwa tingkat fertilisasi pada penambahan 100 ng/mL IGF-I dalam media maturasi nyata lebih tinggi (P<0,05) dibandingkan dengan kontrol dan penambahan 50 ng/mL IGF-I, yaitu berturut-turut 78,3±6,6%, 67,1±8,9%, dan 64,6±6,0% untuk dosis 100, 0, dan 50 ng/mL. Akan tetapi, peningkatan dosis pemberian IGF-I menjadi 150 ng/mL tidak meningkatkan tingkat fertilisasi yaitu 73,5±9,3%. Dari hasil penelitian disimpulkan bahwapenambahan IGF-I dalam media maturasi tidak mampu meningkatkan jumLah oosit yang mencapai tahap MII, namun penambahan 100 ng/mL IGF-I dapat meningkatkan jumLah oosit yang terfertilisasi.
Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG Iman Supriatna; Tuty Laswardi Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 5 No. 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embryo
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Iman Supriatna; Tuty Laswardi yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
PERUBAHAN SEBARAN SEL-SEL ASIDOFIL DAN BASOFIL HIPOFISA PENGARUH PEMBERIAN PASAK BUMI (Eurycoma Longifolia Jack) Hurip Pratomo; Iman Supriatna; Wasmen Manalu; Adi Winarto
Jurnal Matematika Sains dan Teknologi Vol. 12 No. 2 (2011)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/jmst.v12i2.547.2011

Abstract

Pasak bumi is popular as an aphrodisiac in the traditional medical herb. Boiled drink water of the root powder of pasak bumi is believed can increase the secondary reproduction characters such as libido and the duration of erection. This article is part of the research report that focus on: the distribution of hypophysis acidophil and basophil cells caused of the pasak bumi optimal dose treatment. Research carried out in an experiment using Hemaktosilin Eosin(HE) staining method to study micromorphological distribution of the acidophil and basophil cells of the male rat hypophysis after pasak bumi treatment (the dose of 18 mg pasak bumi/200 g body weight during three days, and the control of 1 ml aquadest). Result of the research showed that: Basophyl cells increased significantly in the third days of pasak bumi administered. Meanwhile acidophyl cells decreased in the third days of pasak bumi administered.
FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES . Gholib; Taufiq P. Nugraha; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Antje Engelhardt
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because  it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3α,11β-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.
Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK) Soni Sopiyana; Iman Supriatna; M. Agus Setiadi; Mohamad Fahrudin
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.809 KB) | DOI: 10.14334/jitv.v21i1.1315

Abstract

In poultry embryos, primordial germ cells (PGCs) are progenitor cells for gametes, which have unique migration pathway. Primordial germ cells arise from epiblast in germinal crescent and circulate through the bloodstream for a short period of time, then leave blood vessel to migrate toward gonads. The aim of this study was to determine the potential production capacity of circulated-PGCs of KUB chicken at different developmental stages of embryo using a rapid and simple method. Seventy five KUB chicken fertile eggs were divided into five groups and incubated at 38.5 0C with a humidity of 60%. Hatching was set to the embryonic development stage of 14-18. The blood was collected through dorsal aorta using micropipette under microscope. The collected blood was placed in a 1.5 ml eppendorf tube which was previously filled with 100 µl phosphate buffered saline without Ca2+ and Mg2+ (PBS-) mixed with fetal bovine serum (FBS) with a ratio of 90%:10%. The PGCs were purified using lysis buffer ammonium chloride potassium method. The results showed that average production of circulated-PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P <0.05). The average production of circulated-PGCs at stage 14, 15, 16, 17, and 18 were 37.9; 53.5; 49.8; 38.3; and 33.5 respectively. The number of circulated-PGCs was not different among stages 14, 17 nor 18. The highest number of circulated-PGCs of KUB chicken was obtained at stage 15, so that the isolation and collection of PGCs through the blood circulation was recommended in stage 15.Key Words: KUB Chicken, PGCs, Embryonic Development Stage, Ammonium Chloride Potassium
Follicular dynamic and repeatability of follicular wave development in Peranakan Ongole (PO) cattle Muhammad Imron; Iman Supriatna; . Amrozi; Mohamad Agus Setiadi
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.182 KB) | DOI: 10.14334/jitv.v21i1.1349

Abstract

Superovulation treatment on PO cattle (Bos indicus) was less responsive compared to Bos taurus breed. It might due to the difference of their follicular dynamic. This study was conducted to investigate the follicular dynamics and its repeatability in PO cattle. Follicular dynamics observations conducted on 9 cows trough ultrasound scanning every day. Observations of wave patterns repeatability were performed in 6 cows which its wave pattern already known on the next consecutive IOI.  Research result indicated that PO cattle had 3 (66%) and 4-waves (34%) pattern. The first wave of 3 and 4-waves pattern emerged on day -0.4+0.9 and 1.4+1.1 respectively.  The second wave of 3 and 4-wave pattern emerged on day 9.8+1.5 and 7.4+1.9 respectively.  The pattern of 3 waves has a longer follicle dominant duration (11.6+1.5 day) in the first wave of estrous cycle, compared with 4 waves pattern (10+2.92 and 7+1.00 day respectively). The growth rate of dominant follicle was not different significantly between the 3 and 4-waves pattern (0.87+0.23 and 0.94+0.25 mm/day respectively). Similarly, ovulatory follicle diameter between 3 and 4-waves pattern was also not different significantly (12.24+12.34 and 12.30+12.23 mm respectively). Observation of wave patterns repeatability in 6 PO cows indicated that PO cattle had high repeatability in follicular wave pattern (0.88) and the number of growing follicle was 0.91.  This study resulted data for dynamic of follicular development, wave pattern, its repeatability which be expected to design the protocol of superovulation treatment or other reproduction technologies based on follicular dynamic to improve its result in PO cattle. 
Efektivitas Ekstrak Biji Kapas (Gossypium hirsutum L.) terhadap Jumlah dan Viabilitas Embrio Mencit (Mus musculus L.) Nofri Zayani; Iman Supriatna; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (816.406 KB) | DOI: 10.22146/jsv.27563

Abstract

The cottonseed (Gossypium hirsutum L.) contained gossypol as antifertility agent. The effect of cottonseed extract treatment could be decrease and impaired follicles development were accompanied by oocytesdamage. Damage of oocytes resulted reduction of number and viability of embryos. The objective of this study was to evaluate the effectiveness of cottonseed extract (Gossypium hirsutum L.) on the number and viability ofmice embryo (Mus musculus L.). Doses of the cottonseed extract were used consists of 0 (control), 1.5; 2.1; and 2.7 g/kg of body weight (BW) for 24 days via the oral route. This research used 24 animals healthy of femaleDDY mice 14-15 weeks old and 30-35 g BW. Embryos were collected at day 4 of pregnancy by flushing the utery cornua. The collected embryos were cultured in vitro for 48 hours in modified phosphate buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) culture medium according to the stage of its development to observe the viability of embryos. The result showed that the cottonseed extract with doses 1.5; 2.1; and 2.7 g/kg of body weight (BW) made the number of embryos which collected in D4 of pregnancy significantly lowerthan control (P<0.05). Data from embryos culture in vitro for 48 hours decreased embryos number (P<0.05) that developed in to the expanded and hatched blastocysts. At 2.7 g/kg BW, embryos only can develop to theblastocysts stage. Retardation (4-8 cells) and degeneration embryos did not develop in culture
Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas Siska Adelya Ramadhani; Iman Supriatna; Ni Wayan Kurniani Karja; Adi Winarto
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1258.839 KB) | DOI: 10.22146/jsv.29294

Abstract

Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showedthat the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended.
Respons Superovulasi Sapi Peranakan Ongole terhadap Penyuntikan Tunggal Follicle Stimulating Hormone ke dalam Ruang Epidural (SUPEROVULATION RESPONSES IN ONGOLE CATTLE CROSSBREED TREATED WITH A SINGLE EPIDURAL INJECTION OF FOLLICLE STIMULATING HORMONE) Muhammad Imron; Iman Supriatna; Amrozi .; Mohamad Agus Setiadi
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Super-ovulation is conventionally performed by injection of FSH twice daily for four days. Thistreatment needs frequent attention by farm-personnel and relatively increases the possibility of failuresdue to mishandling and errors in administration of the treatment. This study was conducted to evaluatethe responses of superovulation trough a single injection of FSH into epidural space in ongole cattlecrossbreed. In Experiment 1, a combination of single dose injection of FSH was applied into epidural spaceplus intramuscular (epi+i.m group) compared to the group of intramuscular injection of FSH, which wastreated twice daily for four days (intramuscular group), using equal total dose of FSH 400 mg. Superovulationresponse of epi+im group (n=4) was not significantly different compared with intramusculargroup (n=4). In experiment 2, it compared two treatmentof FSH in different concentration(280 mg versus160 mg) in a single dose applied into epidural space. Data of Epi+im group from experiment 1 was used ascontrol. Group of 280 mg FSH (n=4) resulted total collection of ova/embryo and transferable embryos(9,00+2,65 and 3,33+2,52 respectively) was significantly different compared to the 160 mg group (n=4)(which were 2,00+1,26 and 0,00) P <0,05, although they werenot significantly different compared to thecontrol (9,33+5,68 and 3,67+3,21). In conclusion, injection of a single dose of FSH at 280 mg into epiduralspace result in a comparable transferable embryo which similar to the conventional method that appliedintramuscular injection of FSH twice daily for four days.
Co-Authors . Hasbi . NURBARIAH A Amrozi Abadi, Agung S. Adi Winarto ahmad yani Ahmad Yani Amrozi Amrozi, A Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Aras Prasetiyo Nugroho Arief Boediono Asma, Siti Asri Pudjirahaju Bambang Purwantara Benny Heltonika Cece Sumantri Cutnya’ Shaliran Nazlie (Alm) DEDI CANDRA Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti Mulyawati Kaiin Endang - Gunaisah Eva Handayani Gholib Gholib Gholib Gholib, Gholib Gono Semiadi GONO SEMIADI Gozali Moekti Gustina, Sri Hadi Nurohman Hamid Hamid Hamid Hamid Hasbi . Heistermann, Michael Hismayasari, Intannurfemi Hismayasari, Intannurfemi B. Hismayasari, Intanurfemi B. Hurip Pratomo Hurip Pratomo I Gusti Ayu Budiadnyani I Gusti Wayan Murjana Yasa Intannurfemi B. Hismayasari Intannurfemi Hismayasari Ismail Ismail Ita Djuwita ITA DJUWITA Juli Melia Juli Melia Juli Melia Kresno Suharto Kusdiantoro Mohamad La Ode Syawal Sulaeman Lies Parede Hernomoadi M Agus Setiadi Michael Heistermann Mitha Kurnia Sari Moh. Sayuti Mohamad Fahrudin MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron Muhsuryono Muhsuryono Muhsuryono, Muhsuryono Musthamin Balumbi Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Nofri Zayani Nurbety Tarigan Nurohman, Hadi Pardede, Berlin Pandapotan Prajayanti, Vini Taru F. R. Iis Arifiantini R. Rusli R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rahmatullah Rahmatullah Rahmatullah Rahmatullah Ridwan Affandi RIDWAN AFFANDI Rimas Prathita Agustin Rudy Priyanto Saidin Saidin Saidin, Saidin saidin, saidin Sari, Mitha Kurnia Sayuti, Moh. Sayuti, Mohammad Setiyono, Achmad Siska Adelya Ramadhani Soni Sopiyana Sri Gustina Srihadi Agungpriyono Surya Kusuma Wijaya Suwandi, Syifa Damaianti Syamsiar, Syamsiar Tambajong, Daisy TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Thomas Mata Hine Tuty Laswardi Yusuf Vini Taru Febriani Prajayati Wasmen Manalu Yundari, Yundari