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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ZOO INDONESIA Jurnal Matematika Sains dan Teknologi Jurnal Perikanan Universitas Gadjah Mada Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Zoo Indonesia Jurnal Airaha Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Kefarmasian Indonesia
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Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
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Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Abstract

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.
Perubahan morfo-anatomi dan penyimpanan energi pada fase perkembangan gonad ikan senggaringan, Mystus nigriceps (Valenciennes, 1840) di Sungai Klawing Purbalingga, Jawa Tengah [Morpho-anatomical changes and energy storage during gonadal development of twospots catfish, Mystus nigriceps (Valenciennes, 1840) in Klawing River, Purbalingga, Central Java] Ridwan Affandi; Benny Heltonika; Iman Supriatna
Jurnal Iktiologi Indonesia Vol 11 No 2 (2011): Desember 2011
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v11i2.143

Abstract

A research to explore morpho-anatomcal changes and energy storage on various organs during gonadal development of twospots catfish, Mystus nigriceps was carried out in Klawing River, Purbalingga, Central Java. Morpho-anatomical parameter e.g. condition factor, viscera somatic index, adipose fin index, hepato somatic index, gonado somatic index; and energy content of muscle of dorsal region, visceral organ, adipose fin, liver, and gonad tissue were measured based on gonad development stages. The results showed that morpho-anatomy and energy content in tissues have changed during the period of gonadal development. Moreover, there were a process of storage, transfer and transformation of energetic substances for gonad maturation and spawning activities. AbstrakSuatu penelitian dengan tujuan untuk mengkaji perubahan nilai indeks morfo-anatomi dan penyimpanan energi pada beberapa organ tubuh ikan senggaringan selama masa perkembangan gonad telah dilaksanakan di Sungai Klawing, Purbalingga, Jawa Tengah. Parameter morfo-anatomi meliputi faktor kondisi (CF), indeks jaringan viseral (VSI), indeks sirip lemak (AFI), indeks jaringan hati (HSI), indeks jaringan gonad (GSI), dan kandungan energi pada organ/jaringan otot punggung, sirip lemak, organ viseral, hati, dan gonad telah diukur berdasarkan tingkat perkembangan gonadnya. Hasil penelitian menunjukkan bahwa selama masa perkembangan gonad terjadi perubahan nilai-nilai parameter morfo-anatomi dan kandungan energi pada organ yang diukur, terjadi proses penyimpanan, perpindahan dan perubahan materi berenergi baik untuk keperluan pematangan gonad maupun untuk aktivitas pemijahan.
Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Abstract

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.
The Effect of Vitamin E Supplement in the Diet on Gonad Maturation of Nilem (Osteochilus hasselti, CV) Nurbety Tarigan; Iman Supriatna; M. Agus Setiadi; Ridwan Affandi
Jurnal Perikanan Universitas Gadjah Mada Vol 19, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.26509

Abstract

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving gonad maturation in nilem fish. In this study used a flockof fishbrood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg/kg. The results showed that fortifiedof vitamin E which were in feed significantly affected to the percentage of gonad development, gonadosomatic index (GSI), hepatosomatic index (IHS), fecundity, the diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity was 375 mg kg-1 with six weeks maintenance. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameters of the eggs were 0.31 mm, and the percentage of mature eggs was 64.44%. These results supported the potential of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in nilem fish thereby providing methods to improve fish reproduction
Analisis Karakteristik Bakteri Probiotik Iman Supriatna; Intannurfemi B. Hismayasari; I Gusti Ayu Bidiadnyani; Moh. Sayuti; Ahmad Yani
Jurnal Airaha Vol 5 No 2: Desember 2016
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.916 KB) | DOI: 10.15578/ja.v5i2.30

Abstract

This study aimed to identify and analyze the characterictics of probiotic bacteria. The method used was descriptive method to perform biochemical analyze on the type of probiotic bacteria derived from banana tree. Biochemical analyazed include Gram stain, catalase, oxidation, OF paraffin, OF non paraffin, citrate, ornithin, lysine, urea, MEVP, TSIA oblique, TSIA upright, lactose and sucrose. Results of laboratory analysis showed that the bacteria isolated in blood agar have the morphological characteristic of rod-shaped (bacil), gram-positive, can produce the enzym catalase, but it can not oxidize, it can not produce the enzyme citrate, ornithine, urease, These bacteria are acidic nature, able to ferment lactose and sucrose, and capable of produced acetone from glucose fermentation.
Analisis Konsentrasi DNA dan Prediksi Protein Coral Hamid Hamid; Iman Supriatna; Endang Gunaisah; Muhsuryono Muhsuryono; Ismail Ismail; Abdul Gofir
Jurnal Airaha Vol 4 No 2: Desember 2015
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.896 KB)

Abstract

This study aimed to determine the concentration of DNA and proteins Coral predictions based on data from GenBank. DNA isolation technique Pocillopora sp. using tissue and blood DNeasy Kit (Qiagen). DNA concentrations were analyzed using a spectrophotometer and Corl protein prediction by comparing the primers used by the data GenBank (NCBI). DNA concentration of each sample varies between 5.0 ng/µl-20.8 ng/µl in the first purification and between 3.0 ng/µl - 8 ng/µl, where the highest concentration of DNA present in the sample HC2. DNA concentration and purity of samples obtained at HC2 and HC3. Prediction of protein based on data GeneBank is homologous with a NADH dehydrogenase in Coralliumkonojoi
Analisis C-Type Lectin Iman Supriatna
Jurnal Airaha Vol 4 No 1: Juni 2015
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (876.318 KB)

Abstract

This study aimed to determine the diversity of c-type lectin on the various groups of species based on data contained in National Center for Biotechnology Information (NCBI). The method used is a bioinformatics study by analyzing the amino acid molecules lectin particular type of c-type lectin found in NCBI and analyze of sequence and scores of c-type lectin with Prosite analysis based. The results showed that the data of genbank seen as the varying length of the amino acid sequence types of c-type lectin of groups of species of fish 159-304 aa, bivalves 155-524 aa, plants 349-414 aa, fungi 593-981 aa, and bacteria 564-4379 aa. Prosite analysis results with the c-type lectin on some species have slightly varied score that O. niloticus 18.010, M. meretrik 18, 112, A. thaliana and P. marinus 15,104. Potential C-type lectin on some of these species need to be analyzed further to determine the extraction technique and its benefits in the field of maritime affairs and fisheries.
TINGKAT KEBERHASILAN MOULTING DAN KELULUSAN HIDUP (SURVIVAL RATE) KEPITING BAKAU (Scylla serrata Forskal) DENGAN PERLAKUAN SALINITAS BERBEDA Mohammad Sayuti; Iman Supriatna; Intannurfemi Hismayasari; I Gusti Ayu Budiadyani; Ahmad Yani; Vini Taru P; Hadi Nurohman; Saidin Saidin
Jurnal Airaha Vol 7 No 01: Juni 2018
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.6 KB) | DOI: 10.15578/ja.v7i01.82

Abstract

The existence of the provisions of the Ministry of Marine Affairs and Fisheries that the capture and / or expenditure of crabs (Scylla spp.) with the provisions of the size of the carap above 15 (fifteen) cm or weight above 200 (two hundred) grams per tail becomes a challenge in the soft carapas (soca) crab business that generally using crabs with an average weight below 150 grams. This study aims to determine the success rate of moulting and survival rate crab (Scylla serrata Forskal) with different salinity treatment using crabs weighing above 200 gr. This study used Completely Randomized Design with 5 (five) treatments and used 3 (three) replications, analysis of variance (ANOVA) at 95% confidence level was used to analyzing the data obtained. If there was a significant difference (P <0.05) then proceed with LSD test (Least Significant Differences) with 95% confidence level. The results of this research showed that the highest moulting success rate was obtained from the treatment with salinity of 10 ppt. While the highest survival rate (SR) was obtained from the treatment with salinity 30 ppt.
Potensi Alfa Enolase (ENO1) Membran Plasma Spermatozoa Sapi Bali Sebagai protein Antigenik Teguh Sumarsono; Bambang Purwantara; Iman Supriatna; Mohamad Agus Setiadi; Muhammad Agil
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.262-269

Abstract

Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik.
Nuclear Maturation Competence and Pronuclear Formation in Ovine Oocytes Supplemented with L-ergothioneine in Maturation Medium Rimas Prathita Agustin; Mohamad Agus Setiadi; Iman Supriatna
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3120

Abstract

This study aimed to determine the efficacy of supplementing maturation medium with L-ergothioneine on nuclear maturation competence and pronuclei formation of ovine oocytes in vitro.  In the first study, oocytes were divided into four groups and matured for 24 hours in a maturation medium supplemented with 0 mM (control), 10 mM, 15 mM, and 20 mM LE.  In the second study, oocytes were divided into two groups and matured for 24 hours in a maturation medium supplemented with 0 mM LE (control) and 10 mM LE (optimal dose from the first study), then co-incubated with sperm in a fertilization medium for 12 hours.  The results of the first study showed that the maturation rate of oocytes enriched with LE 10 mM (88.74±1.79%) and 15 mM (87.52±2.30%) in maturation medium was significantly different (P0.05) compared to the control (78.55±1.78%) and LE 20 mM (77.37±1.21%).  Furthermore, the results of the second study revealed that the fertilization rate of oocytes with 10 mM LE (75.55±3.47%) supplementation in maturation medium had a significantly higher percentage of two pronuclei formation than control (63.78±3.49%).  In conclusion, supplementing 10 mM LE in a maturation medium improved oocyte nuclear maturation competence and fertilization rates.
Co-Authors . Hasbi . NURBARIAH A Amrozi Abadi, Agung S. Adi Winarto ahmad yani Ahmad Yani Amrozi Amrozi, A Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Aras Prasetiyo Nugroho Arief Boediono Asma, Siti Asri Pudjirahaju Bambang Purwantara Benny Heltonika Cece Sumantri Cutnya’ Shaliran Nazlie (Alm) DEDI CANDRA Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti Mulyawati Kaiin Endang - Gunaisah Eva Handayani Gholib Gholib Gholib Gholib, Gholib GONO SEMIADI Gono Semiadi Gozali Moekti Gustina, Sri Hadi Nurohman Hamid Hamid Hamid Hamid Hasbi . Heistermann, Michael Hismayasari, Intannurfemi Hismayasari, Intannurfemi B. Hismayasari, Intanurfemi B. Hurip Pratomo Hurip Pratomo I Gusti Ayu Budiadnyani I Gusti Wayan Murjana Yasa Intannurfemi B. Hismayasari Intannurfemi Hismayasari Ismail Ismail ITA DJUWITA Ita Djuwita Juli Melia Juli Melia Juli Melia Kresno Suharto Kusdiantoro Mohamad La Ode Syawal Sulaeman Lies Parede Hernomoadi M Agus Setiadi Michael Heistermann Mitha Kurnia Sari Moh. Sayuti Mohamad Fahrudin MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron Muhsuryono Muhsuryono Muhsuryono, Muhsuryono Musthamin Balumbi Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Nofri Zayani Nurbety Tarigan Nurohman, Hadi Pardede, Berlin Pandapotan Prajayanti, Vini Taru F. R. Iis Arifiantini R. Rusli R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rahmatullah Rahmatullah Rahmatullah Rahmatullah Ridwan Affandi RIDWAN AFFANDI Rimas Prathita Agustin Rudy Priyanto Saidin Saidin Saidin, Saidin saidin, saidin Sari, Mitha Kurnia Sayuti, Moh. Sayuti, Mohammad Setiyono, Achmad Siska Adelya Ramadhani Soni Sopiyana Sri Gustina Srihadi Agungpriyono Surya Kusuma Wijaya Suwandi, Syifa Damaianti Syamsiar, Syamsiar Tambajong, Daisy TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Thomas Mata Hine Tuty Laswardi Yusuf Vini Taru Febriani Prajayati Wasmen Manalu Yundari, Yundari