Article Per Year (5 Year)
p-Index From 2021 - 2026
2.383
P-Index
This Author published in this journals
All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Kebijakan Kesehatan Indonesia Journal of Veterinary and Animal Sciences
Ida Bagus Ngurah Swacita
Fakultas Kedokteran Hewan, Universitas Udayana
Education Medicine & Pharmacology Public Health Veterinary

Published : 37 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 37 Documents
Search

 1   2   3   4 
Studi Histologi dan Histomorfometri Daging Sapi Bali dan Wagyu (HISTOLOGYCAL AND HISTOMORPHOMETRY STUDY OF BALI CATTLE AND WAGYU BEEF) Ni Ketut Suwiti; I Putu Suastika; Ida Bagus Ngurah Swacita; I Nengah Kerta Besung
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.522 KB)

Abstract

The objective of this study was to detect histological structure and histomorphometry of bali cattleand wagyu beef. The samples were collected from rib eye muscle of wagyu and bali cattle. The tissuesamples were fixed, dehydrated and embedded in paraffin and 4-5 mm sections, for histological examinationand stained with Haematoxilin-Eosin (HE). The light binocular microscope was used to identify the samples.The study showed that the beef histological structure consisted of: cells / muscle fibers, fatty and connectivetissues. The muscle fibers of wagyu were observed larger, regular, and longer compared to the Bali cattle.Additionally, intramuscular fat cells and connective tissue interlobulus were found less and thinner thanthe Bali cattle. In the area of muscle where the longitudinal cut was done, its observed that the presence ofI and A bands in Bali cattle beef were clearer compared to the wagyu. The diameter muscle fibers of balicattle beef (45.00 ±1.94 ?m), was smaller (P < 0.01) compared to the wagyu (75.00±1.82 ?m), as well as fatcell size of bali cattle beef (90.10 ±2.13 ?m) and wagyu beef (195.00±2.57?m). In conclusion, the structureof histology and histomorphometry of rib eye muscle in Bali cattle were different to the wagyu.
Pelacakan Ekspresi Antigen Toxoplasma gondii secara Imuno(sito)histokimia (TRACKING EXPRESSION OF TOXOPLASMA GONDII ANTIGENS USING IMMUNO(CYTO)HISTOCHEMISTRY METHOD) Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.568 KB) | DOI: 10.19087/jveteriner.2017.18.4.535

Abstract

The research objective want to track the expression of Toxoplasma gondii antigenes in the heart of the free-range chicken with immuno(cyto)histochemistry technique. In the present study were examined three methods to track antigen T.gondii, first method direct microscopic examination of the heart digests, the second method to detect antigen of T.gondii with immunocytochemistry technique of the chicken heart digests and the third immunohistochemistry examination of the heart free-range chicken. The number of material samples examined were 100 heart free-range chicken. Direct microscopic examination of the heart digests free-range chicken to track the bradyzoite form (inside cyst). Examination by immuno(cyto)histochemistry technique keep track T.gondii an antigen expression on cells and heart muscle tissue. The results showed that the direct microscopic examination on the heart tissue unable to detect cyst and antigen T.gondii. Immunohistochemical examination successfully detected the expression of antigenes T.gondii and was found 2% (2/100) positive. It can be concluded that T.gondii antigen expression in the heart of range chicken could be detected by immunohistochemistry.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.636 KB)

Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
Peranan Pemangku Kepentingan dalam Pengendalian Rabies dengan Pendekatan One Health Terintegrasi di Bali Made Subrata; Sang Gede Purnama; Arya Wahyu Utami; Kadek Karang Agustina; IBN. Wahyu Swacita
Jurnal Kebijakan Kesehatan Indonesia Vol 9, No 1 (2020)
Publisher : Center for Health Policy and Management

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkki.54246

Abstract

Bali sejak 2008 mengalami kasus rabies dan saat ini menjadi daerah endemis rabies. Berbagai cara telah dilakukan untuk pengendaliannya namun kasusnya masih tetap ada. Diperlukan pendekatan one health yang berkolaborasi antara kesehatan manusia, hewan dan lingkungan. Tujuan dari penelitian ini untuk mengetahui tantangan program, peranan pemangku kepentingan dalam pengendalian rabies dan upaya pendekatan one health yang terintegrasi. Penelitian ini menggunakan pendekatan kualitatif untuk mengetahui tantangan program, peranan pemangku kepentingan dan pendekatan one health terintegrasi. Informan dipilih sesuai dengan kebutuhan peneliti. Hasil penelitian menunjukan tantangan dalam pelaksanaan program pada sistem pendataan masih kurang, vaksinasi anjing belum optimal, cara memelihara anjing yang masih diliarkan, program kontrol populasi, manajemen kasus gigitan anjing belum terpadu, edukasi masih rendah, partisipasi masyarakat yang rendah. Perlu peranan dari semua pemangku kepentingan untuk pengendalian rabies. Program pengendalian rabies dapat terlaksana dengan baik apabila mampu mengurangi kesenjangan yang terjadi. Masing-masing kelompok pemangku kepentingan berperan dalam program pengendalian rabies. Melalui pendekatan one health yang terintegrasi dilaksanakan kegiatan yang berkolaborasi dalam surveilan epidemiologi, manajemen kasus gigitan terpadu, manajemen hewan penular rabies dan faktor sosial-budaya-ekologi lingkungan.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.859 KB)

Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Tracking Antigen Expression of Toxoplasma gondii in Mice by Immuno (Cyto) Histochemistry Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p08

Abstract

Mouse has been an animal model that is very sensitive towards the Toxoplasma gondii infection. This research infects 30 mice with free-range chicken heart inoculate and the detection of T. gondiiby immunohistochemistry on the infected mice. The purpose of this study is to track T.gondii antigen expression by immune(cyto)histochemistry in mice. Observation over mice was conducted over 10 weeks. After 10 weeks, the mice are examined for its peritoneal fluid by microscopic and immunohistochemistry examination. Continued by the examination of internal organs to track the expression of T.gondii antigens by immunohistochemistry. The study result showed microscopic examinations on peritoneal fluid did not found any cyst or tachyzoites form of T.gondii. Examination of peritoneal fluid by immunohistochemistry was found to detect expressions of T.gondii antigen. Examinations of the mice’s internal organs detected expressions of T.gondii antigen only in the brain by immunohistochemistry examinations.
Kualitas Daging dan Produk Olahan Daging yang Dijual di Pasar Tradisional Kota Denpasar, Bali Tama, Kevin Tri; Remontara, Al Afuw Niha; Arta, I Komang Wira Kusuma Maha; Dhayanti, Ni Luh Evy; Priharyanthi, Luh Komang Ayu Puteri; Swacita, Ida Bagus Ngurah
Indonesia Medicus Veterinus Vol 12 (3) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.3.351

Abstract

Kualitas daging adalah ukuran yang penting dalam hal palatabilitas dan penerimaan kepada konsumen. Kandungan gizi yang tinggi dalam daging merupakan media yang sangat baik untuk pertumbuhan mikroorganisme sehingga menyebabkan daging mudah rusak dan busuk. Evaluasi ini bertujuan untuk mengetahui kualitas daging dan produk olahan daging dengan menggunakan sampel yang didapat dari pasar tradisional di Kota Denpasar seperti Pasar Batu Kandik, Pasar Poh Gading, Pasar Badung, dan Pasar Kumbasari. Pengujian ini dilakukan di Laboratorium Kesehatan Masyarakat Veteriner dan Epidemiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana. Pengujian dilakukan secara subjektif dan objektif. Uji subjektif daging dan olahan daging meliputi warna, aroma, konsistensi, tekstur, tenunan pengikat, serta kepualaman. Uji objektif daging meliputi uji pH, daya ikat air, kadar air, dan cemaran mikrob. Pengujian daging dan olahan daging menunjukkan bahwa warna, aroma, dan konsistensi dalam beberapa sampel dikategorikan normal sesuai dengan jenis daging dan produk daging, kecuali daging ikan dari Pasar Batu Kandik yang konsistensinya berair dan memiliki aroma amis. Tenunan pengikat dan kepualaman daging sapi dan babi masing-masing memiliki mutu II dan 10%, sedangkan daging ayam dan ikan memiliki mutu I dan 0%. Pada uji objektif, kadar pH daging dikategorikan normal berdasarkan jenisnya berkisar 5,4-6,4, sedangkan produk olahan daging seperti bakso berkisar 6-7 dan sosis 6-6,5; daya ikat air berkisar 64,43-83,75%; kadar air berkisar 71,7-81,9%, dan angka lempeng total bakteri (ALTB) 196 x 103-440 x 103 CFU/g. Namun, pertumbuhan Coliform melebihi batas normal dengan nilai 68-222 CFU/g. Hasil dari pengujian tersebut menunjukkan produk olahan daging memiliki kualitas baik. Seluruh daging dari Pasar Poh Gading dan Pasar Batu Kandik memiliki nilai organoleptik yang baik, kecuali daging ikan dari Pasar Batu Kandik. Seluruh daging memiliki nilai cemaran Coliform di atas batas normal kecuali daging ikan dari Pasar Poh Gading.
Co-Authors A.A. Ketut Agung Cahyawan W Agnes - Tasia Agung Denlira Sitepu Agustina A Naibaho Agustina Lesmauli Nazara Aida Lousie Tenden Rompis Aletha Yuliana Mandala, Aletha Yuliana Alvionita Alexther Lingga Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Anastasya, Sindika Andriawino Berdionis Sanam Anggara Fajri Prasafitra Anjelia Martina Dewi Apriyani, Ester Muki Arta, I Komang Wira Kusuma Maha Christine Regina Fenita Wenno, Christine Regina Fenita DESKI CITRA DWITANIA Desy Ariani Ferdianti, Desy Ariani Dharmawan N.S Dhayanti, Ni Luh Evy DWI SURYANTO Elly Hariati Br Purba, Elly Hariati Br EMI HAMIDAH Gadis Ayu Septyawati Gracemon Loe Mau, Gracemon Gusti Ayu Yuniati Kencana Gusti Made Widyantara I Gede Mahardika I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I K. K. Agustina I Ketut Suada I Ketut Suada I Ketut Suada I MADE ADITYA SASTRAWAN I Made Damriyasa I Made Mahaputra I Made Ricky Dwi Cahya I Made Roby I Made Subrata I Made Sukada I Nengah Kerta Besung I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Suarsana I Nyoman Suartha I Putu Suastika I Putu Sudiantara Cipta I Wayan Masa Tenaya I Wayan Polos I Wayan Suardana I Wayan Suardana I. A. P. Apsasri I. B. Ardana I.A.P. Apsari I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Resmihariningsih Ida Bagus Made Oka Ida Bagus Oka Winaya Iolanda Hermenegildo da Costa Kade Karang Agustina Kadek Karang Agustina Ketut Budiasa Ketut Tono Pasek Gelgel Laras Ayu Nadira Leksana Aditya Kris Nugraha Lely Anggriani Nababan Luh Made Destriyana Luh Made Sudimartini Luh Putu Pradnya Swari M.D. Rudyanto Made Beratha Mukti Martha Putri Manullang Martin Pedro Krisenda Resman MAS DJOKO RUDYANTO Maulina Nababan, Maulina Mita Andini, Mita MURNI SARI Natalia Natalia Nelci Elisabeth Bolla Ni Kadek Deasy Pitriyawati Ni Ketut Suwiti Ni Made Chintya Dewi Sukasari Ni Made Dwi Alita Wulandari Ni Nyoman Citra Susilawati Ni Putu Tessa Arsaning Rahayu Ni Wayan Arya Utami, Ni Wayan Arya Ni Wayan Ayu Rukmini Ni Wayan Suryanadi Pinontoan Kersty Putri Nathania Priharyanthi, Luh Komang Ayu Puteri Purnama Layli, Purnama Putu Aditya Pratama Arta Putra Putu Ayu Sisyawati Putriningsih Putu Henrywaesa Sudipa Remontara, Al Afuw Niha Rima Nurmayani Romy Muhammad Dary Mufa Sabelina Fitriani sang gede purnama Satria Yanuwardani Setiawan Sianturi, Inti Sari Pati R U Sihombing, Vivi Ekatry Stelen, I Dewa Ketut Raeyadi Purnama Suci Wulandari Sumartono - Supriaty Hutasoit, Supriaty T. Sari Nindia Tama, Kevin Tri Tjokorda Sari Nindhia Vinensia Ghona Gani Vinensia Ghona Gani Wayan Tunas Artama Wieke Sri Juniartini Yuli Darmawan, Yuli