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PENGHAMBATAN AKTIVITAS XANTHINE OXIDASE OLEH EKSTRAK ETANOL AKAR SAMBILOTO (Andrographis paniculata, Ness) SECARA IN VITRO Septianingsih, Ulfah; Susanti, Hari; Widyaningsih, Wahyu
Pharmaciana Vol 2, No 2 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.217 KB) | DOI: 10.12928/pharmaciana.v2i2.665

Abstract

Sambiloto root of which contained of flavonoids used by the people fortraditional medicine. In the previous publication, an effective xanthine oxidaseinhibitory activity of flavonoids was reported. In research, in vitro xanthine oxidaseinhibitory activity of etanolic root extract of Andrographis paniculata was determinedand Allopurinol was used as a control. The etanolic extract was succesively extractedin a Soxhlet with petroleum eter. Inhibition of xanthine oxidase by etanolic extract wasmeasured the decrease of uric acid production and monitored by spectrophotometer at295 nm with xanthine as substrat. The enzyme inhibitory activity was calculated, andthen IC50 was determined. The result of analyzed with Kruskal Wallis and MannWhitney at 95% confidental level . The flavonoids of etanolic extract were separated onusing by paper chromatography and the spot changing was determined using UV 366with and without amonia.The result of the research showed that the etanolic extract ofAndrographis paniculata inhibited xanthine oxidase activity with IC50 16,54 µg/mlwhile Allopurinol 4,29 µg/ml. The etanolic extract contained flavon or flavonol.
Pelatihan Pencegahan Penyalahgunaan Narkoba bagi Siswa-Siswi SMA Muhammadiyah 2 dan 3 Yogyakarta Widyaningsih, Wahyu; Wahyuningsih, Iis
JURNAL WIDYA LAKSANA Vol 7, No 1 (2018)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.878 KB) | DOI: 10.23887/jwl.v7i1.11876

Abstract

Penyalahgunaan narkoba pada akhir tahun ini dirasakan semakin meningkat. Angka penyalahgunaan narkoba di Indonesia mencapai kurang lebih 5 juta jiwa, sedangkan di Yogyakarta sudah mencapai 60.182 jiwa pada tahun 2014. Penyalahhgunaan narkoba menjangkiti semua lapisan masyarakat, tidak terkecuali siswa sekolah. Hal ini terjadi biasanya terjadi karena penawaran, bujukan, atau tekanan seseorang atau sekelompok orang kepadanya. Didorong rasa ingin tahu, ingin mencoba, atau ingin memakai, siswa mau menerima tawaran tersebut, yang selanjutnya tidak sulit baginya untuk menerima tawaran berikutnya. Program pengabdian ini merupakan bagian dari Iptek bagi Masyarakat (IbM) dengan tema Swamedikasi Teman Sebaya, bertujuan melatih siswa SMA untuk mengidentifikasi dan mengenal bahaya penyalahgunaan narkoba dan miras di kalangan teman sebaya. Metoda pelaksanaan dilakukan dengan pendidikan masyarakat melalui penyuluhan, pelatihan dan focus group discussion (FGD). Pelatihan diselenggarakan bekerja sama dengan Badan Narkotika Nasional Provinsi DIY. Masyarakat sasaran tanggal 24-25 juli 2017, bertempat di Ruang Multi Media SMA Muhammadiyah 2 Yogyakarta. Tingkat partisipasi peserta 100% dan tingkat kemampuan penguasaan peserta terhadap materi pembelajaran bahaya narkoba meningkat setelah dilakukan pelatihan.
REDUCED ULCEROGENECITY FROM SELF-NANO EMULSIFYING DRUG DELIVERY SYSTEM OF PIROXICAM Wahyuningsih,M.Si., Apt, Iis; Widyaningsih, Wahyu; Wulandari, Silviana
Pharmaciana Vol 8, No 2 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.733 KB) | DOI: 10.12928/pharmaciana.v8i2.11478

Abstract

Piroxicam is antiinflammatory non-steroidal (AINS) drug group that has anti-inflammatory, analgesic and antipyretic effects. Like most other AINS drugs, piroxicam has low solubility and has gastrointestinal (ulcerogenic) side effects on long-term use. The nano-emulsifying drug delivery system (SNEDDS) is one of the technologies that can be used to overcome it. This study aims to determine the effect of ulcerogenic SNEDDS piroxicam compared with piroxicam formulas instead of SNEDDS. This study uses white rats male strain SD age 2-3 months and weight 100-200 grams of 40 rats. Rats divided into 5 groups. Group I was a normal control group, the test animals were given only water. Group II was a suspending control group treated with a 1% polyvinylpyrrolidone (PVP) solution, group III is a carrier control group treated with SNEDDS carrier which is a mixture of tween 80, virgin coconut oil (VCO) and polyethylene glycol (PEG) 400, group IV was a group of piroxicam drugs suspended with piroxicam 1.08 mg/kg in 1% PVP, group V was treated with SNEDDS piroxicam.Treatment was done for 28 days. After treatment, the gastric of rats were taken to be observed for ulcerogenic effects. Observations were made macroscopically by looking at ulcer scores followed by histopathological observations of tissue. The ulcer score data from each group were analyzed using one-way ANOVAand LSD test. The results showed that the normal control group, 1% PVP suspension and carrier group had a ulcer index of 0.0, 0.0 and 0.0, while the piroxicam suspension group and the SNEDDS group had an ulcer index of 0.88 and 0.0. These results were confirmed by histopathologic results of SNEDDS piroxicam to decrease the effect of pyroxicam ulcerogenic with results in the piroxicam suspension group has ulcer with necrosis by neutrophil infiltration, lymphocytes and mast cells in the mucosal tunica to submucosa. In the SNEDDS piroxicam group there is erosion with necrosis of the mucosal tunica epithelium with infiltration of lymphocytes and mast cells in submucosal tunica. It can be concluded the SNEDDS piroxicam can decrease the ulcerogenic effect.
Efek Antioksidan Ekstrak Etanol Ganggang Hijau (Ulva Lactuca L.) Terhadap Kadar Malondialdehid (MDA) Dan Aktivitas Enzim Superoksida Dismutase (SOD) Hepar Tikus Yang Diinduksi CCL4 Widyaningsih, Wahyu; Sativa, Riza; Primardiana, Indra
Media Farmasi: Jurnal Ilmu Farmasi Vol 12, No 2: September 2015
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.965 KB) | DOI: 10.12928/mf.v12i2.3756

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Kerusakan hepar sering disebabkan karena stress oksidatif yang berkaitan erat dengan radikal bebas. Karbon tetraklorida merupakan senyawa toksik sumber radikal bebas penyebab timbulnya stress oksidatif. Adanya kerusakan jaringan tersebut dapat ditandai dengan meningkatnya kadar malonildialdehid (MDA)  dan kenaikan kadar enzim superoksid dismutase (SOD). Ganggang hijau (Ulva lactuca L.) mengandung senyawa melatonin yang berfungsi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol ganggang hijau (Ulva lactuca L.) sebagai antioksidan dengan parameter kadar MDA  dan aktivitas enzim SOD hepar tikus yang diinduksi CCl4. Penelitian ini bersifat eksperimental menggunakan post-test only control group design dilakukan  pada 25 hewan uji yang dibagi menjadi lima kelompok, terdiri dari kelompok I (normal) hanya diberi makan dan minum, kelompok II  diberi CMC-Na 1%, kelompok III (kontrol pembanding) diberi tablet curcuma 200 mg/KgBB, kelompok IV dan V diberi ekstrak etanol ganggang hijau dosis 100 dan 200 mg/KgBB. Perlakuan dilakukan selama 21 hari dan pada hari ke-22 diinduksi CCl4 1,0 ml/KgBB intraperitoneal(i.p) kecuali kelompok I. Setelah 24 jam, hepar tikus diambil dan diukur kadar MDA hepar dengan metode TBARs. Aktivitas enzim superoksida dismutase (SOD) menggunakan metode spektrofotometri.  Analisis data menggunakan One way ANOVA dengan taraf kepercayaan 95% dan dilanjutkan dengan uji LSD. Hasil penelitian menunjukkan bahwa kadar MDA hepar meningkat  dan aktivitas enzim SOD menurun secara signifikan akibat induksi CCl4 (p<0,05). Tablet curcuma dosis 200 mg/kgBB, ekstrak etanol ganggang hijau dosis 100 dan 200 mg/KgBB dapat menurunkan kadar MDA dan meningkatkan aktivitas SOD hepar tikus yang diinduksi CCl4 secara signifikan (p<0,05). Kesimpulan penelitian ini adalah ekstrak etanol ganggang hijau (Ulva lactuca L.) mempunyai aktivitas antioksidan dengan menurunkan kadar MDA dan meningkatkan aktivitas enzim SOD hepar tikus yang diinduksi CCl4. Kata kunci :  Ulva lactuca L., melatonin, antioksidan, MDA, SOD
AKTIFITAS HEPATOPROTEKTIF EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) DILIHAT DARI RASIO BERAT HEPAR, NILAI SGPT-SGOT, DAN HISTOPATOLOGI HEPAR PADA TIKUS SPRAGUE DAWLEY YANG DIINDUKSI CCL4 Irham, Lalu Muhammad; Widyaningsih, Wahyu
Media Farmasi: Jurnal Ilmu Farmasi Vol 14, No 1: Maret 2017
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.463 KB) | DOI: 10.12928/mf.v14i1.9826

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Hepar merupakan organ yang berperan dalam pengaturan homeostasis tubuh meliputi metabolisme, biotransformasi, sintesis dan imunologi. Penyebab penyakit hepar bervariasi, diantaranya adalah virus, obat-obatan, alkohol dan stress oksidatif yang merupakan faktor utama. Jumlah radikal bebas yang melebihi antioksidan menyebabkan terjadinya stres oksidatif. Sidaguri (Sida rhombifolia L.) mengandung senyawa fenol dan flavonoid yang berkhasiat sebagai antioksidan. Penelitian ini bertujuan untuk menguji efek hepatoprotektif ekstrak etanol daun sidaguri (EEDS) terhadap nilai SGPT-SGOT, gambaran histopatologi hepar tikus dan rasio berat hepar yang diinduksi carbon tetrachloride (CCl4). Dua puluh lima tikus dibagi menjadi 5 kelompok. Kelompok I merupakan kelompok normal yang hanya diberi makan dan minum, kelompok II merupakan kelompok kontrol yang diinjeksi CCl4 secara intraperitoneal dengan dosis 1 mL/KgBBdan kelompok (III, IV dan V merupakan kelompok perlakuan yang diberi EEDS peroral dengan dosis: 25, 50 dan 100 mg/KgBB) berturut-turut selama 21 hari. Pada hari ke 21 kelompok ini diinjeksi CCl4 1 mL/KgBB secara intraperitoneal 1 jam setelah perlakuan. Data rasio berat organ hepar dari masing masing kelompok dianalisis secara statistik menggunakan analisis varian (ANOVA), LSD post hoc test P<0,05 untuk menentukan perbedaan signifikan. Hasil penelitian menunjukkan bahwa rasio berat hepar pada kelompok II (kontrol) 216,52±15,04 berbeda signifikan dibandingkan dengan kelompok III, IV dan V berturut turut dengan nilai 436,56± 46,78; 438,86±44,79; dan 534,46±45,49. Hal ini menunjukkan bahwa pemberian EEDS dapat menaikkan rasio berat hepar. Data ini didukung dengan nilai SGPT dan SGOT yang berbeda bermakna dengan kelompok kontrol (p<0,05). Dilihat dari gambaran histopatologi hepar, pada  kelompok  II (kontrol), III, IV dan V berturut-turut tidak  menunjukkan adanya nekrosis karena CCl4. Kesimpulan dari penelitian ini bahwa EEDS dapat melindungi hepar berdasarkan rasio berat hepar dan nilai SGPT-SGOT, namun belum dapat disimpulkan bahwa EEDS memiliki efek hepatoprotektif dilihat dari gambaran histopatologi.
SKRINING FITOKIMIA EKSTRAK ETANOL Ulva lactuca L. DENGAN METODE KROMATOGRAFI LAPIS TIPIS Widyaningsih, Wahyu; Pramono, Suwidjiyo; Widyarini, Sitarina; Sugiyanto, Sugiyanto
Media Farmasi: Jurnal Ilmu Farmasi Vol 13, No 2: September 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (679.042 KB) | DOI: 10.12928/mf.v13i2.7772

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Ganggang hijau (Ulva lactuca L.) merupakan spesies ganggang laut yang berpotensi sebagai obat kardiovaskuler. Untuk mendukung pemanfaatannya perlu dilakukan skrining fitokimia kandungan ganggang hijau yang berasal dari pantai Drini, Daerah Istimewa Yogyakarta. Tujuan penelitian ini adalah melakukan skrining fitokimia Ulva lactuca yang berasal dari pantai Drini dengan Kromatografi Lapis Tipis (KLT). Ulva lactuca diekstraksi dengan metode maserasi dengan etanol 96%. Ekstrak yang diperoleh ditetapkan randemen,  kadar air, kadar abu dan kadar logam berat (Pb dan Cd). Penetapan kadar air dengan alat Hydrogen Moisturizer Analyzer (HMA), kadar abu dengan metode gravimetri dan penetapan logam berat Pb dan Cd dengan Spektrofotometer Serapan Atom (SSA). Skrining fitokimia dilakukan untuk mengindentifikasi golongan senyawa alkaloid, flavonoid, saponin, antrakuinon dan terpenoid dengan KLT. Identifikasi klorofil dilakukan dengan metode Nollet (2004). Hasil penelitian menunjukkan ekstrak etanol Ulva lactuca  mempunyai randemen 14,52%, kadar air 9,67±0,07%, kadar abu 34,05% , kadar logam Pb<0,07 mg/kg dan kadar logam Cd<0,01 mg/kg. Terdapat golongan senyawa flavonoid, alkaloid dan klorofil dalam  ekstrak etanol Ulva lactuca L. yang diperoleh dari pantai Drini.
Hepatoprotective Activity of Ethanolic Extract of Sida rhombifolia L. Leaf in CCl4-induced Rats Nisa Isneni Hanifa; Wahyu Widyaningsih
Acta Pharmaciae Indonesia : Acta Pharm Indo Vol 8 No 2 (2020): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2020.8.2.2929

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Streptococcus mutans is responsible for cause the dental caries. The purpose of this research was to test antibacterial activity of Ganitrus leaves. The bacterial activity of methanol and aquadest by disc difusion method with 10%; 20%; 30%; 40%; 50% and 100%. The extracts showed that methanol extract had activity with susceptible category at concentration of 10%, 30%, 50% and 100% against Streptococcus mutans with inhibition zone 15.74mm, 16.68 mm, 16.70 mm and 17.68 mm, respectively. Aquadest extract showed activity with intermediet category of 30% and 100% with inhibition zone 11,04 mm and 11,39 mm. The methanol extract showed that activity of antibacterial better than the aqueous Elaeocarpus ganitrus Roxb leaves.
Gastroprotective effect of Canna edulis Ker. ethanolic extract in piroxicam-induced rats Dara Pranidya Tilarso; Moch. Saiful Bachri; Wahyu Widyaningsih
Pharmaciana Vol 11, No 1 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.027 KB) | DOI: 10.12928/pharmaciana.v11i1.18063

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Medications derived from plants and pure natural ingredients have far lower side effects and risks than synthetic drugs. One side effect of piroxicam is irritation of the digestive tract. One of the therapeutic preventions to minimize peptic ulcers was utilizing Canna edulis Ker. This study aims to prove that ethanolic extract of Canna edulis Ker. can be used as an alternative to prevent piroxicam-induced peptic ulcers based on ulcer index parameters and the protection ratio. The rats were divided randomly into 6 groups consisting of 5 rats. The normal group was given food and water. The negative control group was given 0.5% CMC-Na. The extract groups were given various doses of ethanolic extract of Canna edulis Ker. (50, 100, and 200 mg/kg BW). The positive control was given sucralfate at 360 mg/kg BW dose. Rats were treated orally for 14 days. One hour after the treatment on the 14th day, all groups except group I were orally administered with piroxicam dissolved in 0.5% CMC-Na at 1.8 mg/kg BW dose. Twenty-four hours later, animals were sacrificed, dissected, and their stomach organs were removed to analyze its number of ulcers. Ulcer observation was formed by giving a score and protection ratio. The mean ulcer index value was 0.33±0.58 for 50 mg/kg BW and 100 mg/kg BW ethanol extract treatment groups, while the 200 mg/kg BW group showed 0.67±0.58. The protection ratio was 83.33±28.87 in 50 mg/kg BW and 100 mg/kg BW treatment groups, while  66.67±28.87 was shown in the 200mg/kg BW group. Canna edulis Ker. ethanolic extract has the gastroprotective ability by decreasing the index of gastric ulcers and increasing the protection ratio to the stomach of piroxicam-induced rats.
Incision wound healing activity of free-range chicken (Gallus domesticus) egg white gel in mice Wahyu Widyaningsih; Sapto Yuliani; Ayu Wulandari; Rifka Salsabila
Pharmaciana Vol 11, No 3 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.152 KB) | DOI: 10.12928/pharmaciana.v11i3.20531

Abstract

Egg whites reportedly exhibit wound healing activities by forming new cell tissues and accelerating damaged cell tissue recovery. This study aimed to determine whether and how egg whites prepared in gel dosage forms healed incision wounds in mice (Mus musculus). The method began with formulating gels by mixing egg whites with Carbopol 940, methylparaben, propylparaben, propylene glycol, triethanolamine, and distilled water. Sixteen mice were divided evenly into four groups, and each was subjected to a 1 cm long midline dorsal incision. Group I was given 10% povidone-iodine ointment, group II was gel base, group III was 10% egg white gel, and group IV was 20% egg white gel. Based on the test parameters measured during macroscopic observation, namely wound length and healing time, the gel preparations were found to fulfill the physical properties requirements, and incision wounds treated with 10% and 20% egg white gels healed in 9 and 12.5 days, respectively. The statistical test revealed a significant difference in wound size on Day 10 and in healing time between wound treatments with 10% gel and base gel (p < 0.05). Therefore, it can be concluded that the 10% egg white gel is effective in healing cuts in mice as it speeds wound healing and decreases wound size.
UJI AKTIVITAS PENGHAMBATAN POLIMERISASI HEME (1)-N-(2-NITROBENZIL)-1,10- FENANTROLINIUM IODIDA DAN (1)-N-(4-NITROBENZIL)-1,10- FENANTROLINIUM IODIDA SECARA IN VITRO Laela Hayu Nurani; Dwi Utami; Wahyu Widyaningsih; Iin Narwanti; Eti Nurwening; Jumina Jumina
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.12 KB) | DOI: 10.12928/pharmaciana.v4i2.1575

Abstract

The inhibitory activity of heme polymerization of (1)-N-(2-nitrobenzyl)-1,10- phenantrolinium iodide and (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide have been done. This study aims to analyse the (1)-N-(2-nitrobenzyl)-1,10-phenantrolinium iodide and (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide as inhibitory of polimerization heme. Analysis of heme inhibtory polimerization activity used the experimental in vitro method. The activity showed by IC50 (the capable concentration of extract to inhibiting polymerization heme by 50% ). The IC50 value acquired by probit analysis. Assess IC50 of (1)-N-(2-nitrobenzyl)- 1,10-phenantrolinium iodide not to be identified, (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide and chloroquine by successively are 0,571±0,071; 25,498±1,876 mg/mL. The result showed the (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide had the highest value of the heme polymerization inhibitory activity than chloroquin, (1)-N-(2-nitrobenzyl)-1,10- phenantrolinium iodide hadn’t the heme polymerization inhibitory activity.
Co-Authors Aditya Prabowo Aditya Prabowo Ainiyah, Nadia Putri Aldri Frinaldi Amelia Regina Arsyad Anasyua, Fairuz Khairunnisa arsyad, regina Astutik, Novita Santi Ayu Wulandari Azis Ikhsanudin Bachri, Mochammad Saiful Br. Sebayang, Sri Rosepda Cahya, Sella Nur Danang Prasetyaning Amukti Dara Pranidya Tilarso Daru Estiningsih Dedi Kuswandi Desi Eko Wulansari Desi Iswahyuni Desyandri Desyandri Dini Rahmawati Dwi Fitriani Dwi Retno Nengtyas Dwi Utami Erliyana, Mila Eti Nurwening Sholikhah Fadilah, Nur Aini fatima, fatma Fauzi, Muh Fajar Fauziyyah, Khusna Fitriyati, Laeli Galang Pergiawan Ramadhan Hanifah, Azka Khansa Hari Susanti Iin Narwanti Iin Narwanti Iis Wahyuningsih Indra Primardiana, Indra Irfan Yusuf Iriwi L.S Sinon, Iriwi L.S Iriwi L.S. Sinon, Iriwi L.S. Istyarini Istyarini, Istyarini Jumina Jumina Jumina Jumina Kaffah, Silmi Kintoko Kusuma, Rio Jati Laela Hayu Nurani Laela Hayu Nurani Lalu Muhammad Irham M. Paden, Thereziana marpaung, Tio Widia A. Moch Saiful Bachri Moch Saiful Bachri Moch. Saiful Bachri Moch. Saiful Bachri Muhammad Ma'ruf Muhammad Yusron Maulana El-Yunusi Mujasam Mujasam Nina Salamah Nisa Isneni Hanifa Nur Asih, Nur Nurliawaty, Lilis Pramono, Suwidjiyo Primardiana, Indra Puteriragil Atma Pertiwi, Puteriragil Atma Putri Dwi Lestari Radjab Sutrisno, Radjab Ramadhan, Muhammad Marwan Rerung, Nensy Rezza Wahyudi Rifka Salsabila Riska Riska Riza Sativa, Riza Rumansara, Tamariska Herlindo Ryan Syahputra Sapto Yuliani Sapto Yuliani Sativa, Riza Septianingsih, Ulfah Silmi Kaffah Siswanti Siswanti Sitarina Widyarini Siti Fatmawati Fatimah, Siti Fatmawati Sri Rosepda Sebayang, Sri Rosepda Suci Kurniati Sugiyanto - Sugiyanto, Sugiyanto Sumiasih Sumiasih Suwidjiyo Pramono Tio Widia Astuti Marpaung Tirza Pangkali, Tirza Ulfah Septianingsih Vivi Sofia Vivi Sofia Wiki Yuli Anita Wiki Yuli Anita Wulandari, Silviana Wulandari, Silviana Wulansari, Widyanari Febriyana Wumu, Dea Anggrainy Y.T. Allo, Alberto Yenusi, Kaleb Amaghi Yoga Yuniadi