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Purnama Sukardi
Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman
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EFFECTS OF PHOTOPERIODS ON THE CLONED GtH GENES IN HARD-LIPPED BARB (Osteochilus hasseltii) Prayogo, Norman Arie; Pramono, Taufik Budi; Siregar, Asrul Sahri; Sukardi, Purnama; Kawaichi, Masashi
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.3.1100

Abstract

Photoperiod is one of the factors that regulate endocrine gland hormone production necessary for gonadal growth and development, gametogenesis, and reproductive cycles in fish. However, studies on the influence of photoperiod on tropical fishes are still limited. Therefore, this study investigated the effects of photoperiod on the hard-lipped barb, particularly the gene expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II). The experiment was designed using three treatments: 14L:10D (control), 8L:16D (short photoperiod), and 18L:6D (long photoperiod). Four aquariums with nine fish per tank were used as replicates. The fish were maintained under these photoperiod treatments for eight weeks. Pituitary activity was observed by measuring GtH gene expression. The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursor encoded by the cDNA consisted of 67 amino acids, including the mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were applied. The results indicated that photoperiod length affects the reproductive performance of the hard-lipped barb.
Spirulina platensis DIET FOR MILKFISH, Chanos chanos, LARVAE Sukardi, Purnama; Yansah, Noprie; Winanto, Tjahyo; Marnani, Sri; Prayogo, Norman Arie; Harisam, Taufan; Sudaryono, Agung
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.841 KB) | DOI: 10.11598/btb.2019.26.3.1103

Abstract

In aquaculture, Spirulina platensis is used as a feed supplement as it contains the amino acid phenylalanine. This study was conducted to evaluate the differences in the effects of spirulina-based microcapsules and commercial diets on the absolute, daily, and specific growth rates, as well as the survival rates, of milkfish larvae. The larvae were fed with Spirulina platensis as a core diet in microcapsules with different matrix (wall) compositions. The first capsule wall consisted of gelatin and fish oil, while the second capsule wall contained gelatin, fish oil, and whole egg. The control group was fed a commercial diet. A total of 1,200 larvae were used in this experiment using a recirculating system. The experiment was conducted over 42 days of culture. The larvae were fed three times per day, and the feed amount was increased regularly in accordance with larval growth. The results showed that the effects of both spirulina-based microcapsule diets on absolute growth rate (AGR), specific growth rate (SGR), and average daily growth rate (ADGR) of Chanos chanos larvae were comparable to those fed a commercial diet. The survival rates were 80.6 ± 11.17% for larvae fed Spirulina platensis with a gelatin and fish oil wall, 84.6 ± 8.44% for those fed Spirulina platensis with a gelatin, fish oil, and whole egg wall, and 83.8 ± 16.50% for those fed the commercial diet. This study demonstrated that spirulina-based microcapsules had similar effects to commercial feed on the growth of milkfish larvae, indicating that this diet could potentially replace commercial feed.
GENETIC IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA Harisam, Taufan; Haryono, Florencius Eko Dwi; Marhaeni, Bintang; Amron, Amron; Prayogo, Norman Arie; Sukardi, Purnama
BIOTROPIA Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2020.27.1.1105

Abstract

Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.
Implementasi Inovasi Probiotik Berbasis Limbah Pasar untuk Meningkatkan Produktivitas Ikan Nila di Kelompok Krido Yuwono Mina Padi Technopark, Panembangan, Banyumas Sujadi, Frentina Murti; Anjaini, Jefri; Simangunsong, Tohap; Setiyaningsih, Lilik; Mardika, Hanisya Putri Kania; Setyawan, Agung Cahyo; Sukardi, Purnama; Kusuma, Baruna; Nurhabib, Asro; Marnani, Sri; Harvinda, Yohanes
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13355

Abstract

The probiotic innovation training programme based on market waste implemented at the Krido Yuwono Mina Padi Technopark Group aims to increase tilapia productivity and farming efficiency through the use of organic waste as raw material for probiotics. This training involves participants in the production of probiotics through the fermentation of market waste using lactic acid bacteria starters. The evaluation results show an increase in tilapia productivity of around 15-20% with better feed efficiency and a reduction in ammonia levels in pond water, which was previously a major problem in maintaining fish health. The community involved in this programme also increased their confidence in managing tilapia farming efficiently and received new knowledge that can reduce operational costs. This programme has achieved significant social and economic impacts and is expected to develop into a sustainable community empowerment model in the field of aquaculture and support more environmentally friendly waste management.
Edukasi Penerapan Autofeeder dan Manajemen Kualitas Air Berbasis Energi Surya pada Budidaya Minapadi Desa Penembangan, Banyumas Anjaini, Jefri; Nurhabib, Asro; Sukardi, Purnama; Setiyaningsih, Lilik; Ekowati, Nur Alfi; Agung Tri Nugroho
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13785

Abstract

POKDAKAN (Kelompok Pembudidaya Ikan) memiliki peran penting dalam pengembangan budidaya perikanan berkelanjutan, termasuk sistem integrasi minapadi. Namun, tantangan yang dihadapi adalah keterbatasan energi listrik di area persawahan serta pemberian pakan yang masih manual, yang menyebabkan efisiensi produksi belum optimal. Kegiatan pengabdian masyarakat ini dilaksanakan pada tanggal 20 September 2025 di Desa Penembangan, Banyumas, Jawa Tengah oleh tim dosen Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman. Tujuan kegiatan adalah meningkatkan kapasitas POKDAKAN melalui edukasi, pelatihan, pendampingan, dan transfer pengetahuan dalam penerapan sistem otomatisasi pakan berbasis energi surya. Metode yang digunakan meliputi sosialisasi, edukasi, wawancara, kuesioner, serta replikasi teknologi. Hasil kegiatan menunjukkan peningkatan pengetahuan dan keterampilan anggota POKDAKAN, peningkatan efisiensi pakan sebesar 20–25%, serta kesiapan pembudidaya untuk mereplikasi sistem di lahan lain. Program ini membuktikan bahwa edukasi berbasis teknologi dapat meningkatkan kemandirian pembudidaya sekaligus mendukung praktik budidaya ramah lingkungan.
Penerapan Auto Feeder dan Sistem Monitoring Kualitas Air Real-Time Berbasis Tenaga Surya pada Budidaya Minapadi Terintegrasi POKDAKAN Kridoyuwono, Panembangan, Banyumas Sukardi, Purnama; Anjaini, Jefri; Lilik Setiyaningsih; Nur Alfi Ekowati; Agung Tri Nugroho; Frentina Murti Sujadi; Asro Nurhabib; Baruna Kusuma; Yohannes Harvinda; Kholilah Nur Hidayah; Dandi Setio Wibowo; Dimas Yoland Dewantio
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13857

Abstract

Panembangan Village has strong potential for rice–fish culture (minapadi) but faces constraints in feeding efficiency, unmonitored water quality, and dependence on grid electricity. This community‐service program offers an integrated solution: an IoT-based auto feeder combined with real-time water-quality monitoring (DO, pH, temperature, conductivity/TDS, turbidity) and powered by off-grid solar energy (PV) to ensure continuous operation. The primary goal was to improve feed efficiency, enhance fish growth, reduce feed waste, and optimize water quality management in aquaculture systems. The implementation of these technologies has demonstrated significant improvements in feed conversion ratios, growth rates of fish, and overall water quality management. The program also provided training to local farmers, enhancing their capacity to manage these technologies effectively. This initiative is expected to contribute to the sustainable development of aquaculture in the region, benefiting both the local community and the environment
KONFIRMASI INTEGRATIF MORFOLOGI DAN DNA BARCODING IKAN SEBELAH YANG KEKURANGAN DATA DI PERAIRAN CILACAP, INDONESIA: INTEGRATIVE MORPHOLOGICAL AND DNA BARCODING CONFIRMATION OF THE DATA-DEFICIENT INDIAN HALIBUT IN CILACAP WATERS, INDONESIA Hartono, Sugeng; Haryati, Ani; Salsabila, Sahda; Adiyanto, Fajar; Sukardi, Purnama; Puspito, Gondo; Abdulrahman, Idris
Jurnal Teknologi Perikanan dan Kelautan Vol. 17 No. 1 (2026): FEBRUARY 2026
Publisher : Fakultas Perikanan dan Ilmu Kelautan, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24319/jtpk.17.126-138

Abstract

The Indian halibut (Psettodes erumei) is classified as data deficient on the IUCN Red List. Accurate species identification is a fundamental prerequisite for sustainable fisheries management and biodiversity conservation. This study aims to identify the Indian halibut landed from Cilacap waters, Indonesia, using an integrative morphological and molecular approach. Four specimens collected in March and June 2025 were analyzed using an integrated morphological and molecular approach. Morphological identification was based on detailed morphometric and meristic analyses, cross-referenced with standard taxonomic guides. Molecular identification involved DNA extraction, PCR amplification of the mitochondrial Cytochrome C Oxidase Subunit I (COI) gene, electrophoresis, sequencing, and comparison with global genetic databases using BLAST. Morphological characteristics aligned with the description of P. erumei, though significant morphometric differences from a historical reference suggest potential local phenotypic variation. DNA barcoding confirmed the identity with 100% sequence similarity to conspecifics from other Indo-Pacific regions (GenBank accession: PX599017.1). Phylogenetic analysis further placed the Cilacap samples within a genetically homogeneous cluster of P. erumei from across Indonesian waters. This research successfully contributes to Indonesia's biodiversity database and DNA barcode library for a commercially exploited yet understudied species. It also provides essential baseline data to inform stock assessments and conservation strategies in the region.
Co-Authors , Hartoyo, , -, Andreas Eka Saputra . HERNAYANTI Abdulrahman, Idris Abednego Dwi Septiadi Achmad Iqbal Adiyanto, Fajar Agung Sudaryono Agung Tri Nugroho Agus Margiwiyatno Agus Nuryanto ahmad, ananda Amron Amron, Amron Ani Haryati, Ani Anisur Rosyad Arif Yulianto Asrul Sahri Siregar Athari, Hafizha Nur Bessho, Yasumasa Dandi Setio Wibowo Dimas Yoland Dewantio Ekasanti, Anandita Eko Setyo Wibowo, Eko Setyo Emyliana Listiowati, Emyliana Endang Hilmi Fitriadi, Ren Frentina Murti Sujadi Gondo Puspito Harisam, Taufan Harisman, Taufan Haryono, Florencius Eko Dwi Heratri, Agnes Insyira, Athalla Rania Jarot Santoso Kasprijo, Kasprijo Khoerudin, Fatah Kholilah Nur Hidayah Kurnia, Dhimas Ragil Kusuma, Baruna Lilik Setiyaningsih Lilik Setiyaningsih Mardika, Hanisya Putri Kania Mardiyana Mardiyana Marhaeni, Bintang Masashi Kawaichi, Masashi Muhammad Riady, Reza Mulandari, Kusriyah Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nur Alfi Ekowati Nurhabib, Asro nurhafid, mohmammad Nurul Anwar Pramono, Taufik Budi Rijal, Muhammad Azharul Rose Dewi Rosita, Rita Eka Salsabila, Sahda Selia Irawati, Maghfira Setyawan, Agung Cahyo Soedibya, Hary Tjahja Soedibya, Petrus Hary Tjahja Sorta Basar Ida Simanjuntak Sri Marnani Sugeng Hartono Susanto Susanto Syakuri, Hamdan Syuraihanah, Khotoh Taufik Budhi Pramono Tjahjo Winanto, Tjahjo Tobari Tohap Simangunsong Trianingtyas, Laela Wahyu Andi Saputra Wibowo, Satrio Haryu Wijaya, Rudy Winanto, Tjahyo Yansah, Noprie Yohanes Harvinda Yunida, Rizka Yusup, Dandi Diana