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Hasria Alang
Universitas Patompo
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Journal : BIOEDUSCIENCE

 1 
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.134 KB) | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
Co-Authors . Hastuti Abdul Rahim Adelia Adelia Adelia Triwahyuni ADRIANI ADRIANI Ahmad Hasyim Aldri Frinaldi Anastasia Susanti Jimin Annisa Nasywa Fahira Annisa Rahmawati Baihaqi, M. Fiqri Baihaqi, Muh. Fiqri Baihaqi, Muhammad Fiqri Dinar, Yuliana Dwi Febrianty Ningsih Eka Apriyanti Ester Ayu Fahira, Annisa Nasywa Fitr Fitri Fitri , Fitriagustiani, Fitriagustiani Garlan Prayoga Hafsah Hafsah Hafsah Hafsah Hamdani, Ibnu Mansyur Hartini Hartini Hartini, Hartini - Hartini, Hartini - Haryanto Haryanto Hastuti Hastuti Hastuti Hastuti Hastuti Hastuti Hastuti Hastuti Hastuti Hastuti Hasyim, Ahmad Ikramullah, Muh. Chaeril Indri Erwhani Islamawati Islamawati Jagad, Nur Jati Jamhari Jamhari Joni Kusnadi Khaidarsyah Khaidarsyah Khairillah, Yuyun Khairillah, Yuyun Nisaul Marpani Marpani Mas'ati Mas'ati Mashudi Mashudi Merlis Nirmala Putri Muh. Yunus Muhammad Yunus Nabila, Faika Naima Haruna Nasir, Agung Ninda Novita Ningsih, Dwi Febriani Ningsih, Dwi Febrianti Ningsih, Dwi Febrianty Niska E Sitaba Novita, Ninda Nuning Astuti Nur Alifah Nur Amaliah Akhmad Amaliah Akhmad Nur Indah Sari Nurul Fuady Adhalia H Nuruniyah, Nuruniyah Pasmawati, Pasmawati Pratama, Kharisma Pratama, Sandi Fransisco Purnamawati, Ditha Astuti Putri, Sartika Gunawan Rafika Rafika Rafika Rafika Ramanda, Galih Dwiki Ravi Aulia Febrianty Rezeki Amaliah Safira, Pretty Saparina, Saparina Sari, Rayana Meta Selvi Rosalia Siska Bin Basso St. Rahmadani Suharjono Suharjono Suharjono Suharjono, Suharjono Sultan Adi Pratama Surahman Nur Surtikanti Surtikanti Sutrisno Syamsiara Nur Syamsuri Syamsuri Syamsuri Tekla Ganul Tri Ardyati Tri Ardyati Triwahyuni, Adelia Try Ardyati Ube Dagonala Utami, Anggraini Putri Wiwik Wiji Astuti Wulandari, Rizty Yusal, Muh Sri Yuyun Nisaul Khairillah