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Dede Mahdiyah, Dede
AKBIDv Sari Mulia
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Isolasi Bakteri Dari Tanah Gambut Penghasil Enzim Protease Mahdiyah, Dede
JURNAL PHARMASCIENCE Vol 2, No 2 (2015): JURNAL PHARMASCIENCE
Publisher : JURNAL PHARMASCIENCE

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Abstract

ABSTRAK Tanah gambut terbentuk dari hasil dekomposisi bahan-bahan organik dalam keadaan anaerob. Tanah gambut memiliki karakteristik fisika dan kimia yang dapat mempengaruhi tingkat kesuburan gambut. Mikroorganisme pada tanah gambut beranekaragam dan memiliki peranan penting sebagai dekomposer, penyedia unsur hara bagi tanaman, penghasil enzim. Peranan enzim sangat penting bagi industri makanan, obat, pertanian, dan peternakan. Salah satu enzim yang tersebar luas dan peranananya cukup baik dalam industri adalah protease. Protease merupakan biokatalisator untuk reaksi pemecahan protein. Enzim ini akan mengkatalisis reaksi hidrolisis, yaitu reaksi yang melibatkan unsur air pada ikatan spesifik substrat. Penelitian ini bertujuan untuk mengisolasi bakteri dari tanah gambut penghasil protease. Metode penelitian yang dilakukan adalah pengambilan sampel tanah gambut, isolasi bakteri dari tanah gambut menggunakan media Nutrient Agar (NA) dengan melalui teknik pengenceran dari 10-1 sampai 10-7 pada media NaCl 0,9%, setelah itu uji protease menggunakan media NA dan susu skim 1%, untuk identifikasi bakteri menggunakan teknik pewarnaan Gram. Diperoleh hasil dari teknik isolasi bakteri dari tanah gambut sebanyak 180 isolat yang tumbuh baik dengan suhu inkubasi 37oC selama 24 jam. Isolat yang potensial sebagai penghasil protease sebanyak lima isolat yaitu isolat 3TG, 4TG, 5TG, 6TG, dan 7TG yang ditunjukkan dengan adanya zona bening disekitar koloni selama 48 jam. Kelima isolat tersebut berdasarkan morfologi koloni yaitu memiliki bentuk bulat, bulat tidak beraturan, elevasi cembung, cekung dan datar, warna koloni putih, krem, kuning, kuning transparan dan orange. Berdasarkan uji pewarnaan Gram kelima isolat tersebut empat isolat merupakan bakteri Gram positif dan satu isolat yaitu 7TG termasuk Gram negatif. Kata Kunci: Isolasi, Bakteri, Tanah Gambut.ABSTRACTPeat soil is formed from the decomposition of organic materials in anaerobic state. Peat soil has physical and chemical characteristics that can affect the level of fertility of peat. Microorganisms in diverse peat soil and has an important role as decomposers, provider of nutrients for plants, producing enzymes. The role of the enzyme is very important for the food industry, medicine, agriculture, and livestock. One enzyme is widespread and the have role quite well in the industry is a protease. Protease is catalisator biologyst to the reaction of protein breakdown. This enzyme catalyzes the hydrolysis reaction, the reaction involving water element on a substrate specific binding. This study aimed to isolated the bacteria from producing peat soil protease. Research methodology were peat soil sampling, isolation of bacteria from peat using media Nutrient Agar (NA) by the technique of dilution from 10-1 to 10-7 in 0.9% NaCl media, after the test protease using NA and media skim milk 1%, for the identification of bacteria using Gram staining technique. The results obtained from the isolation of bacteria from the peat soil of 180 isolates that grow well with incubation temperature of 37oC for 24 hours. Isolates potential as a producer of protease five isolates that isolates 3TG, 4TG, 5TG, 6TG, and 7TG indicated by a clear zone around the colony for 48 hours. Fifth isolates were based on morphology of the colonies which have a spherical shape, irregular round, elevation convex, concave and flat, colony color white, beige, yellow, transparent yellow and orange. Gram staining test is based on the five isolates, four isolates are Gram-positive bacteria and one isolate that 7TG including Gram negative. Keywords: bacteria, isolate, peat soil.
Optimalisasi Partisipasi Aktif Mahasiswa Melalui Experiental Learning Pada Program Merdeka Belajar Kampus Merdeka Dona, Sismeri; Mahdiyah, Dede; Rahmawati, Dwi; Nurdin, Fauji
Journal of Community Development Vol. 5 No. 2 (2024): December
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v5i2.256

Abstract

21st century learning can develop students' skills needed to live and work in a dynamic global era. The 21st century learning design can be carried out using research methodology based on experiential learning & collaborative practice. Strengthening 21st century skills can also be done using an experiential learning approach that is integrated with the Independent Campus Learning Program launched by the government. The method used in this service is the experiential learning method with stages, namely: preparation stage, observation stage, planning stage, activity implementation stage and evaluation stage of the process that has been undertaken and reflection stage. The target of this service is junior high school students in the Banjarmasin area. The final result of this activity is instilling student empathy towards the problems of community life, being able to work together across fields of science, and increasing the real role and contribution of universities and students in national development.
Effect of gymnastics elderly on the changes of osteoarthritis pain Widodo, Heriadi; Redjeki, Dwi Sogi Sri; Mahdiyah, Dede; Irawan, Angga; Al Kahfi, Rina
Health Sciences International Journal Vol. 2 No. 1: February 2024
Publisher : Ananda - Health & Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71357/hsij.v2i1.19

Abstract

Background: Osteoarthritis is a chronic joint disorder where there is a process of attenuation and disintegration of joint cartilage accompanied by bone growth and new cartilage in the joints. Patients with osteoarthritis occur at the age of 40-60 years; one of the roles of nurses in improving the quality of life of the elderly is to overcome health problems that commonly occur in the elderly. One of them is joint pain management due to osteoarthritis by using non-pharmacological therapy in the form of elderly exercises. Objective: Identify the influence of elderly gymnastics on changes in osteoarthritis pain in social institutions Tresna Werdha Budi Sejahtera Banjarbaru. Method: This study uses a quantitative method with experimental quasi with the research design used is a pretest-posttest one group design. Initial data collection before and after intervention. Results: Respondents who experienced knee ostearthritis pain before elderly exercise can be seen that most respondents have characteristics of moderate pain. The biggest complaint presentation was experiencing moderate pain, which was 30 people (100%) on the first day. Wilcoxon test results found the quality of knee osteoarthritis pain in the elderly before and after a significant compress. This is based on the p-value of 0.000, which means that there is an influence of elderly gymnastics on the reduction in the quality of knee osteoarthritis pain in the elderly in social care institutions because there is prosperity in Banjarbaru. Conclusion: There is a relationship between the effect of elderly gymnastic therapy to reduce osteoarthritis of knee pain suffered by the elderly.
Evaluation of the early detection program for cervical cancer using the see-and-treat method: Evaluasi program deteksi dini kanker serviks dengan metode see and treat Dewi, Candra Ratna; Palimbo, Adriana; Mahdiyah, Dede
Health Sciences International Journal Vol. 2 No. 2: August 2024
Publisher : Ananda - Health & Education Foundation

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Abstract

Background: Cervical cancer is the second most common type of cancer among women in Indonesia after breast cancer and is the third leading cause of cancer death. Objective: Evaluating the implementation of the early detection program for cervical cancer using the see-and-treat method at Martapura 2 Community Health Center. Method: The research design uses quantitative descriptive methods. Sampling used random sampling techniques and obtained 23 health workers as respondents. Data collection was carried out using a checklist sheet. Data were analyzed using univariate analysis. Results: The results of the study showed that all respondents (100%) stated that they had carried out coordination, had not provided training to cadres (100%), Health Workers who had attended training in early detection of cervical cancer (13%), had provided education to the community about cervical cancer (87%), and counseling about the HPV vaccine (61%), there were no problems during the VIA examination (78%), the completeness of the cryotherapy examination equipment met the standards (100%), there were no problems during the implementation of cryotherapy (100%), supported the procurement of the HPV vaccine (96%), but only (22%) proposed the procurement of the vaccine, the majority of respondents stated that patients with IVA results with extensive lesions > 75% were willing to be referred (87%), and stated that there were no obstacles in the referral system (91%). Conclusion: Implementing the early detection program for cervical cancer using the see-and-treat method at Martapura 2 Community Health Center needs improvement.
Effect of demonstration-method counseling on the understanding of correct breastfeeding techniques among pregnant women Fatmawati; Meldawati; Palimbo, Adriana; Mahdiyah, Dede
Health Sciences International Journal Vol. 2 No. 2: August 2024
Publisher : Ananda - Health & Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71357/hsij.v2i2.25

Abstract

Background: Exclusive breast milk is food with nutritional content most suitable for the baby's needs, protects against various infections, and provides a loving relationship that supports all aspects of the baby's development, including the baby's health and intelligence. The success of breastfeeding depends on the understanding of the breastfeeding technique used by the mother. Objective: Analyzed the effect of demonstration-method counseling on understanding correct breastfeeding techniques among pregnant women. Method: The research used a quasi-experimental design with pre- and post-tests. Based on established criteria, 55 samples were taken purposively from 133 pregnant women who underwent examinations at the Muara Koman Community Health Center. Demographic data and pregnant women's understanding of breastfeeding techniques were collected using questionnaires. Analysis was carried out using SPSS. Results: Counseling with demonstration methods for pregnant women can increase understanding of correct breastfeeding techniques. This is shown in the pretest results before being given demonstration method counseling with a score of 30 (52.6%), increasing to a score of 51 (89.5%). Comparative analysis of the pre-and post-tests showed a significant effect of counseling using the demonstration method on the level of understanding of pregnant women about correct breastfeeding techniques with a p-value <0.05. Conclusion: Counseling with a suitable demonstration method is provided to increase pregnant women's understanding of breastfeeding correctly.
Effect of consuming lemongrass (Cymbopogon citratus) on shortening the postpartum period Meidawati, Gusti Sophia; Rahmawati, Dwi; Noval; Mahdiyah, Dede
Health Sciences International Journal Vol. 2 No. 2: August 2024
Publisher : Ananda - Health & Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71357/hsij.v2i2.26

Abstract

Background: Complementary care in some midwifery communities has become important to midwifery practice. One of the reasons why complementary midwifery care is currently widely used is the desire to avoid the side effects of drugs. Based on the birth register at Independent Practice of Midwife Darmawati from September to December 2023, the average number of births was 25 patients a month, with postpartum visit data recorded as 89% of postpartum mothers consuming lemongrass decoction (Cymbopogon citratus) during the postpartum period. Lemongrass is a natural resource proven to speed up the healing process. Lemongrass is rich in potassium, which stimulates blood circulation, improves blood flow, and helps increase endurance. Objective: The aim is to determine the effect of consuming lemongrass decoction on shortening the postpartum period. Method: This research method uses a quasi-experimental research design with a treatment group and a control group design. The population of this study was all postpartum mothers recorded in the IPM Darmawati birth register from September 2023 to January 2024. This research used a tool in the form of an observation sheet which was filled out by respondents in the treatment group every time they drank lemongrass decoction according to the manual for making lemongrass decoction and how to consume it. Results: In the intervention group 25% had the postpartum period in the normal category, and 75% in the fast category, while in the control group 100% were in the normal. The Wilcoxon test results show a significant difference between the treatment and control group with a p-value of 0.042. Conclusion: Consuming lemongrass decoction significantly shortened the postpartum period.
Effect of warm water compress with endorphin massage on reducing labor pain intensity in the first stage active phase Sumartila; Palimbo, Adriana; Istiqamah; Mahdiyah, Dede
Health Sciences International Journal Vol. 2 No. 2: August 2024
Publisher : Ananda - Health & Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71357/hsij.v2i2.27

Abstract

Background: Pain during childbirth is subjective and influenced by various factors, including psychosocial factors, habits and women’s perception of the meaning of childbirth. Uncontrolled labor can harm the mother and fetus. Excessive pain increases anxiety and restlessness in pregnant women, causing increased catecholamine secretion. Non-pharmacological complementary therapy techniques will be used to reduce labor pain. Objective: To determine the effect of warm water compresses with endorphin massage on reducing labor pain intensity during the first stage active phase. Method: This type of quantitative research is a one-group pretest-posttest design with 15 mothers in the first stage of labor as participants, obtained using total sampling. Warm water compress and endorphin massage are performed simultaneously for 20 minutes. Labor pain data was collected using the Wong Baker Faces Scale instrument. Non-parametric statistical analysis using the Wilcoxon sign rank test. Results: The characteristics of the 15 respondents include 11 people (73.3%), 11 people (73.3%), seven people (46.7%), 10 people (66.7%), 10 people in the multiparity category, and 13 people in the 20-35 year age category (86.7%). The results of the Wilcoxon sign rank test showed that giving a warm water compress combined with endorphin massage had a significant effect (p-value <0.05) on reducing the intensity of labor pain in the first active phase. Conclusion: Warm water compresses combined with endorphin massage showed a positive influence in managing the intensity of the pain scale in the first stage of labor
Overcoming anemia in prospective brides and grooms as an effort to prevent stunting Palimbo, Adriana; Bamegawati, Ika Melinda; Mahdiyah, Dede; Herawati, Anita; Rahman, Luthfia Hidayati; Fariana, Yuni Riska Nur; Arfiah
Health Sciences International Journal Vol. 2 No. 2: August 2024
Publisher : Ananda - Health & Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71357/hsij.v2i2.28

Abstract

Background: Before the wedding, the majority of brides to be, still lack knowledge about reproductive health in families. After marriage, pregnancies are often not planned properly. This can have negative impacts such as the risk of pregnancy complications, disability, and even death of the mother and baby. Case presentation: In Banjarmasin, the number of prospective brides and grooms who suffer from anaemia is 7.9% and malnutrition is 11.2. Increasing a person's knowledge and attitudes regarding anaemia prevention by providing behavior and compliance in consuming iron supplement tablets or blood supplement tablets can reduce the risk of pregnancy complications and prevent stunting. Community handling was carried out together through the Midwifery Project implemented in January 2024 at the Pekauman Community Health Center. The instrument used leaflets, banners, and control cards to monitor the consumption of blood supplement tablets. Discussion: Information and educational counseling and demonstrations were used as a learning model for seven pairs of prospective brides or grooms or a total of 14 subjects. This tool uses leaflets, banners, and control cards to monitor the consumption of blood supplement tablets. Conclusion: This project has been proven to increase the bride and groom's knowledge about anaemia, its prevention and treatment, and increased compliance with taking blood supplement tablets regularly as recommended by health services.
EDUKASI MASYARAKAT TENTANG DAGUSIBU OBAT SKABISIDA TOPIKAL DI DESA GUDANG HIRANG Mahdiyah, Dede; Mukti, Bayu Hari; Redjeki, Dwi Sogi Sri
JURNAL PENGABDIAN AL-IKHLAS UNIVERSITAS ISLAM KALIMANTAN MUHAMMAD ARSYAD AL BANJARY Vol 9, No 1 (2023): AL-IKHLAS JURNAL PENGABDIAN
Publisher : Universitas Islam kalimantan MAB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/jpaiuniska.v9i1.8229

Abstract

Skabies adalah penyakit kulit yang disebabkan oleh tungau yaitu Sarcoptes scabiei varietas hominis yang menyebabkan masalah kesehatan masyarakat di wilayah beriklim tropis dan subtropis. Permetrin merupakan salah satu obat yang dapat digunakan untuk terapi skabies. Saat ini, masyarakat masih sering salah dalam mendapatkan, menggunakan, menyimpan, dan membuang obat dengan benar (DAGUSIBU). Oleh karena itu, perlu diadakan kegiatan pengabdian masyarakat untuk memberikan edukasi. Kegiatan pengabdian masyarakat ini bertempat di Desa Gudang Hirang RT 02. Peserta atau sasaran kegiatan adalah warga Desa Gudang Hirang RT 02.  Tujuan kegiatan pengabdian ini adalah untuk memberikan informasi dan edukasi kepada masyarakat mengenai DAGUSIBU obat skabisida topikal. Metode pelaksanaan kegiatan yang dilakukan yaitu identifikasi masalah, intervensi berupa pemberian edukasi dengan ceramah dan diskusi tanya jawab, kemudian melaksanakan evaluasi hasil kegiatan dengan memberikan pre-test serta post-test. Pemberian materi meliputi definisi, prevalensi, etiologi, patofisiologi, pengobatan dan efek samping penyakit skabies serta cara mendapatkan obat dengan benar, cara menggunakan obat dengan benar, cara menyimpan obat dengan benar dan cara membuang obat dengan benar. Pengetahuan masyarakat mengalami peningkatan setelah diberikan edukasi tentang DAGUSIBU obat skabisida. Masyarakat Gudang Hirang memperoleh pengetahuan yang baik tentang DAGUSIBU obat skabisida.
Aktivitas Antibakteri Akar Kayu Bangkal (Nauclea subdita) Terhadap Staphylococcuc aureus Mayasari, Vita; Mahdiyah, Dede; Melviani, Melviani; Nastiti, Kunti
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

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Abstract

Senyawa bioaktif tanaman bangkal (Nauclea subdita) menghasilkan senyawa metabolit sekunder seperti tanin, fenolik, steroid dan senyawa alkaloid. Tetapi belum ditemukan bukti penelitian ilmiah yang menyatakan bahwa akar tanaman bangkal (Nauclea subdita) dapat digunakan sebagai antibakteri. Menguji aktivitas antibakteri ekstrak akar kayu bangkal (Nauclea subdita), konsentrasi hambat minimun (KHM) ekstrak akar kayu bangkal (Nauclea subdita) dan konsentrasi bunuh minimun (KBM) ekstrak akar kayu bangkal (Nauclea subdita) terhadap Staphylococcus aureus. Penelitian eksperimental sesungguhnya (True Eksperimental Research) untuk mengetahui aktivitas antibakteri ekstrak etanol akar kayu bangkal (Nauclea subdita) terhadap bakteri Staphylococcus aureus. Desain penelitian Posttest-Only Control Group Design. Posttest-Only Control Group Design dilakukan secara kelompok eksperimen maupun kelompok control, baik dalam kelompok eksperimen maupun kelompok kontrol akan dibandingkan yang dimana kelas eksperimen akan mendapatkan perlakuan sedangkan kelas kontrol tidak mendapatkan perlakuan. Akar kayu bangkal (Nauclea subdita) terhadap Staphylococcus aureus, memiliki kemampuan sebagai antibakteri terhadap Staphylococcus aureus dengan zona hambat 13,2 mm yang termasuk kedalam kategori zona hambat kuat sesuai hasil skrining aktivitas antibakteri serta memiliki kemampuan daya hambat (KHM) pada konsentrasi 100 mg/L dengan nilai sig. Kruskal-Wallis Test adalah 1.000 dan nilai signifikansi pada Mann-Whitney Test adalah 1.000. Akar kayu bangkal (Nauclea subdita) tidak memiliki kemampuan daya bunuh (KBM) terhadap Staphylococcus aureus. Penelitian ini menunjukkan bahwa akar kayu bangkal (Nauclea subdita) memiliki kemampuan sebagai antibakteri terhadap Staphylococcus aureus, tetapi tidak memiliki kemampuan daya bunuh (KBM) terhadap Staphylococcus aureus.
Co-Authors Achmad Jaelani Adriana Palimbo, Adriana Al Kahfi, Rina Ali Rakhman Hakim Anita Herawati, Anita Arfiah Aryvia, Nabilla Salsa Bamegawati, Ika Melinda Bayu Hari Mukti Budiwinata, Winarta Darsono , Putri Vidiasari Darsono, Putri Vidiasari Dewi, Candra Ratna DWI RAHMAWATI Dwi Sogi Sri Redjeki Dzul Hidayani, Yunisa Fadhiyah Noor Anisa Fahrianti Fahrianti, Fahrianti Fakhriah, Siti Nurintan Fariana, Yuni Riska Nur Fatmawati Fatmawati Fitri Yuliana, Fitri Hariadi Widodo, Hariadi Hidayani, Yunisa Dzul ilhamiyah, Ilhamiyah Irawan, Angga Ismiadi, Rahmad Istiqamah Kasmayuda, Muhammad Lathifah, Nur Lestari, Yayuk Puji Marselino, Doni Irawan Prancisco Mayasari, Vita Meidawati, Gusti Sophia Meldawati Melviani, Melviani Mia Audina, Mia Mohammad Basit, Mohammad Mohtar, M. Sobirin Nastiti, Kunti Nita Hestiyana, Nita Noval Noval Noval Novita Novita Nur Hidayah Nurdin, Fauji Nurlinda Nurlinda, Nurlinda NURUL HIDAYAH Pryhandini, Dwi Sukma Puteri, Puteri Putri Vidiasari Darsono, Putri Vidiasari Rahayyu, Rini Rahmadani Rahmadani Rahmadani Rahmah, Lailatul Rahman, Luthfia Hidayati Ratna Sari Rohama, Rohama Saniah, Husnus Sismeri Dona, Sismeri Sumartila Theana, Milka Tumanggor, Agustina Hotma Uli Tuti Alawiyah Widaningsih, Neni Widodo, Heriadi Winda Maolinda, Winda Yulianingsih, Devi Yustian, Alifira Adhany Zulliati Zulliati Zulliati, Zulliati