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All Journal HAYATI Journal of Biosciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Kedokteran (Journal of Medical Science) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Perspektif : Review Penelitian Tanaman Industri Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Elkawnie Majalah Obat Tradisional Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan Acta Pharmaceutica Indonesia JSFK (Jurnal Sains Farmasi & Klinis) JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Farmasi Indonesia Perspektif, Review Penelitian Tanaman Industri Current Research on Biosciences and Biotechnology Nusantara Hasana Journal Archives Pharmacia Proceeding of Mulawarman Pharmaceuticals Conferences Scientific Journal Journal of Applied Agricultural Science and Technology Jurnal Farmasi Sains dan Praktis Jurnal Natural Riset Informasi Kesehatan AGRIC Jurnal Pengabdian Masyarakat Kesehatan (JURABDIKES) Borneo Journal of Pharmacy Kartika : Jurnal Ilmiah Farmasi Journal of Tropical Pharmacy and Chemistry
Elfahmi Elfahmi, Elfahmi
School of Pharmacy, Bandung Institute of Technology
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Journal : Indonesian Journal of Pharmaceutical Science and Technology

 1 
The transformation of dbr2 and p19 Genes into Artemisia annua L. by Agrobacterium tumefaciens Mediation: Metabolites Analysis Sy, Khairunnisa; Kristianti, Tati; Suhandono, Sony; Elfahmi, Elfahmi
Indonesian Journal of Pharmaceutical Science and Technology 2024: Suppl. 6, no. 3 (The 3rd Mandala Waluya International Conference on Pharmaceutical Science and
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v6i3.54535

Abstract

The low level of Artemisinin in Artemisia annua L. is associated with its biosynthetic pathway. Genetic engineering offers a potential solution to increase its metabolites. The research objectives were to transform the double bond reductase (dbr2), one of the critical genes in the biosynthesis of Artemisinin, and p19, an anti-silencing gene, into A. annua L. by the mediation of Agrobacterium tumefaciens and assess their impact on metabolites production. The dbr2 and p19 genes were verified by analyzing PCR products. Plasmids pCAMBIA1303-DBR2-P19 were transformed into A. tumefaciens and subsequently introduced into the leaves from tissue culture of A. annua L, using vacuum infiltration and assessed by GUS assay. UPLC-ESI-MS/MS measured artemisinic acid (AA), dihydroartemisinic acid (DHAA), and Artemisinin contents. The transformation in A. tumefaciens was successful using a freeze-thaw method. The tissue culture of A. annua L. has been infected by A. tumefaciens using vacuum infiltration. Based on the GUS histochemical assay, the gene has been successfully inserted in the leaves with an efficiency of 48.2%. The result from UPLC-ESI-MS/MS showed that the level of Artemisinin in the transformation sample with and without pCAMBIA-dbr2-p19 was detected but not quantifiable while in wild-type leaves were quantified at 0.008% in fresh weight. The AA and DHAA were not detectable but only in the wild type. The transformation was successful, but the quantification of DHAA and AA was unsuccessful because of the low quantity in the samples.
Co-Authors Adella Shindy Pratiwi Adji Mustiaji Agus Chahyadi Agus Chahyadi Agus Chahyadi Agus Supriadi Aisyah, Siti Zanuba Akbar Dana Akhirul Kahfi Syam Ana Indrayati Andi Rifki Rosandy Andre Ditya Maulana Lubis Andreanus A Soemardji Anggardiredja, Kusnandar Anita K Ari Sr Windyaswari Ari Sri Widyaswari Ari Sri Windyaswari Ari Sri Windyaswari, Ari Sri Ariranur Haniffadli ARTRI Astrid Indalifiany Ayu, Inna Puspa Bashari, Muhammad Hasan Beginer Subhan Budipramana, Krisyanti Choirul Huda Dana, Akbar DEBBIE SOEFIE RETNONINGRUM Desyane, Heipy Ketrin Dondy Arafat FAHRAUK FARAMAYUDA Fahrauk Faramayuda Fahrauk Faramayuda, Fahrauk Fany Mutia Cahyani Fitri Yasa, Yanti Frangky Sangande Ghozaly, Muchammad Reza Heipy Ketrin Desyane Jaka Permana Khairunisa Harizqi Nurul Husna Khairunnisa Sy KOMAR RUSLAN Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Kusnandar Anggardiredja Laode M.R. Al Muqarrabun Laode M.R. Al Muqarrabun Lusi Putri Dwita Manalu, Meilina Marsinta Maria Immaculata Iwo Maria Immaculata Iwo Maria Immaculata Iwo Marlia Singgih Wibowo MARLIA SINGGIH WIBOWO Marlin Megalestin Raunsai Meilina Marsinta Manalu Morawati, Soufni Muamar Abdillah Nabila K Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Niken Tunjung Murti Pratiwi Novriyandi Hanif Nurinanda Prisky Qomaladewi Nurul Nadiah Binti Abdul Rahman Oktiyas Muzaky Luthfi Pratiwi Ramadhani Feriza Rachmat Mauludin Ramelan, Riska Sigit RENALDI OKTAVIANUS Retno Wahyuningrum Retno Wahyuningrum Ridzka Magfirah Rika Amran Rika Hartati Riska Sigit Ramelan Rissyelly Rissyelly Rozida Khalid Sahidin Sahidin Sani, Lalu M. Iqbal Santoso, Winny Sartika Yuniarti Shinta Maulida Rosandhy Sigid Pamungkas Wicaksono Silvy Julian Soemardji AA Sony Suhandono SONY SUHANDONO Sony Suhandono Soraya Riyanti Soraya Riyanti Sufyan Zainul Arifin Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sulistiarini, Riski Sultan Salahudin Jamal Sumirtapura, Yeyet Cahyati Syafrizayanti, Syafrizayanti Syaikhul Aziz Syefira Salsabila Tan MI Tati Kristanti TATI KRISTIANTI Tina Rostinawati Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Tri Septiana, Vina Tri Yuspita Sari , Jenny Tri Yuspita, Jenny Weni Widy Astuti Winny Santoso Wirasutisna, Komar Ruslan Wirasutisna, Komar Ruslan Wiwin Herdwiani Yasa, Yanti Fitri Yeni Karlina Yodha, Agung Wibawa Mahatva Yolazenia Yolazenia Yolisa Fitri, Vioni Yosie Andriani Yuspita Sari, Jenny Tri Zaini Alfahmi