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Journal : Narra J

Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
Co-Authors Aan Andriyansyir Saputra Aaqilah Hanaan Arianti Abdurasulov, Abdugani Achmad Luqmanul Hakim Adinda Ayu Noveriera Adjen Falah Muhammad Adnyana, I MDM. Aflacha Alivia Risalatul I Afnani, Moh R. Agusniar Furkani Listyani Agusniar Furkani Setiyawati Al Husnah, Syahdah Iksiroh Alya Rahmaningrum Alya Rosyidah Putri Ramadhani Ananda Rigi Aditya Andiani Andiani Andiani, Andiani Andra Agnez Al Aska Angelica, Gusti Ayu Gresia Angga Putra Surya Rahmadhani Anggaresta, Dewa Putu Kresna Ansori, Arif NM. Aqliyah, Fithratil Ardhi Wanda Arisdinata, Frederica Amelia Putri Ayu Artha Putri, Awanda Dias Rizkia Atik Sri Wulandari Atmaja, Peter Y. Aulia Putri, Desmatika Ayly Soekanto Ayu Cahyani Noviana Bayu Prakoso, Bayu Cesarea, Haning Chatterjee, Shrabonti Choiro, Anis Nurun Christin Merlina Wattimury Claudia Key Deby Rahmayanti Denik Putri Nabilasari Derkho, Marina Devi Dwi Rianti, Emilia Dharmayani, Ni Made Gita Gayatri Diana Diana Dikry Sada Amaltullah Dilla Dayana Putri Dordya Paquita Wijaya Dorta Simamora Durry , Fara Disa Durry, Fara D. Dwiari, Ni Made Mawar Edwin Edwin Efendi, Wiyoga Agung Eka Wulandari Emanuel, Endrayana Putut Laksminto Emilia Devi Dwi Rianti Emilia, Nur F. Emillia Devi D.Rianti, Emillia Devi Erny Erny Erny Erny Ethica, Stalis N. Eufemia, Eufemia Fairus Firdani Azzaky Fara Disa Durry Firdous, Athika Fuad Ama Gandhi I., I Gusti Agung Bagus GEDE ABI YOGA Gede Abi Yoga Gede Vito Chanda P Gegel Purnomo Wicaksono Gere Yusuf Kelly Giri, Made Widiantara Hanna Windyantini Hannah Izzati Hardiyono Hardiyono Hardiyono Hardiyono Hendrawan, Yucky Hendriansyah, Muhammad Hafizh Herawaty, Wike Hesty Mustika Rachmah I Komang Adhi Arya Yudanta I Made Krisna Naradha I Putu Agus Aris Wijaya Kusuma I Putu Oktayana Idwar, Herizal Ima Arum Lestarini Jamaluddin, Indra P. Jordhy Akbar KADEK DWIPA Kadek Dwipa Kadek Gede Dhiyo Bhargo Yasa Kadek Rachmawati Kadek Rio Risnanda Kadriyan, Hamsu Kartika Ishartadiati Keysha Amira Hanny Khairullah, Aswin R. Kumara Danta, I Made Kusuma, I Gusti Bagus Dharma lailatul jannah Latifunisa, Shabira Hasna Lilis Sulistyorini Lionesya Sukma Winata Listyawati, Agusniar Furkani Lusiani Tjandra M. Raffli Naresca Pradana M. Rizky Cahyo M.Raffli Naresca Pradana Mahata, Joydeep Mahayutha, I Putu Bagus Yustitio MANDASARI DWI PERTIWI Mandasari Dwi Pertiwi Maria Juliati Kusumaningtyas Masfufatun Masnuna W., Diti Nabilah Mochammad Mirza Arif Pranata Mohammad Irgi Hamdani Mukarimatussa’ada, Alva Murtadlo, Ahmad AA. Musa, Susan H. Nadilah Amanda Naw, Sin W. Ni Kadek Niken Ayu Lestari Ni Luh Komang Ayu Hertina Sintara Ni Made Shinta Putri Kesumantara Ni Putu Santhi Cahya Dewi Nike Salindri Niken Triyulianita S Niluh, Niluh Nofa, Ilham NOVA NOVITA Noveriera, Adinda Ayu Novia Restu Windayani, Novia Restu Noviana, Cahyani Nur Anggraini, Dian Eka Nur Khamidah Nurachmawati Meindah Sari Nurlaili, Efi Nurul Yaqin Nurwasilah Nurwasilah, Nurwasilah Nutdfah Nurmiftsa Palupi, Hana Indi Tisyria Cipto Parikesit, Arli A. Permatasari, Kiswati Rozi Pinatih, Januartha Putra Prada Marwah Al Kamilah Pranaya, Randy Hadyan Prasetya, Johan Danu Prasetyaning Estu Pratiwi Putri Listia, Cory Artania Putu Ayu Aryanda Parawidnyaningsih Putu Oky Ari Tania Putut Laksminto Emanuel R. Azizah Rachmawati, Anisa Nur Rahadian Zainul Rahma, Salsabila Rani Nur Rafli Amalia Rayhanah Nadzifiah Rebezov, Maksim Reisner Reza Hesty Viona Rismawaty, Ika Rollando, Rollando Rovik, Anwar Ruby Riana Asparini Safitri, Warini Ayu Salsabila, Elsa Salsabila, Puteri Sandra Miladyna Sanusi, Obed Nelson Satriyo Nugroho, Satriyo Sella Dela Hanna Putri Aziz Shita, Shita Siti Hadijah Sofyana, Nuril Maulidah Sri Lestari Utami Sri Masyeni, Dewa Ayu Putri Sucipto Sudharma, I Putu Bagus Arya Triesta Mystah Istyadzah Utami, Sri Lestari Viona Rindu Pristia Wahyuni Dyah Parmasari, Wahyuni Dyah Wijaya, Putu Listya Ayu Wike Herawaty Wike Herawaty Winata, Lionesya Sukma Yanuar Rizka Nuraida Yosephi, Valensa Yudistira, I Kadek Alam Yuniekawati, Yuyun Yusuf Badrus Zahida, Farah Zahrah Aswa Ananta Zulfan Febriawan Zulfan Febriawan Zulfi Zaskia Ramadhini