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Journal : Narra J

Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
Co-Authors Achmad Luqmanul Hakim Adjen Falah Muhammad Agusniar Furkani Listyani Agusniar Furkani Setiyawati Al Husnah, Syahdah Iksiroh Andiani Andiani Andiani, Andiani Andra Agnez Al Aska Angelica, Gusti Ayu Gresia Angga Putra Surya Rahmadhani Anggaresta, Dewa Putu Kresna Ansori, Arif NM. Aqliyah, Fithratil Arisdinata, Frederica Amelia Putri Ayu Artha Putri, Awanda Dias Rizkia Atik Sri Wulandari Atmaja, Peter Y. Aulia Putri, Desmatika Ayly Soekanto Bayu Prakoso, Bayu Choiro, Anis Nurun Derkho, Marina Devi Dwi Rianti, Emilia Dharmayani, Ni Made Gita Gayatri Diana Diana Dikry Sada Amaltullah Dilla Dayana Putri Dorta Simamora Durry , Fara Disa Durry, Fara D. Dwiari, Ni Made Mawar Edwin Edwin Efendi, Wiyoga Agung Eka Wulandari Emanuel, Endrayana Putut Laksminto Emilia Devi Dwi Rianti Emillia Devi D.Rianti, Emillia Devi Erny Erny Erny Erny Ethica, Stalis N. Eufemia, Eufemia Fara Disa Durry Fuad Ama Gandhi I., I Gusti Agung Bagus GEDE ABI YOGA Gede Abi Yoga Giri, Made Widiantara Hannah Izzati Hardiyono Hardiyono Hardiyono Hardiyono Hendrawan, Yucky Hendriansyah, Muhammad Hafizh Herawaty, Wike I Putu Agus Aris Wijaya Kusuma I Putu Oktayana Ima Arum Lestarini Jamaluddin, Indra P. KADEK DWIPA Kadek Dwipa Kadek Rachmawati Kadriyan, Hamsu Kartika Ishartadiati Keysha Amira Hanny Kumara Danta, I Made Kusuma, I Gusti Bagus Dharma Latifunisa, Shabira Hasna Lilis Sulistyorini Listyawati, Agusniar Furkani Lusiani Tjandra M. Rizky Cahyo Mahayutha, I Putu Bagus Yustitio MANDASARI DWI PERTIWI Mandasari Dwi Pertiwi Maria Juliati Kusumaningtyas Masfufatun Masnuna W., Diti Nabilah Mohammad Irgi Hamdani Mukarimatussa’ada, Alva Murtadlo, Ahmad AA. Musa, Susan H. Naw, Sin W. Ni Luh Komang Ayu Hertina Sintara Ni Made Shinta Putri Kesumantara Nike Salindri Niluh, Niluh Nofa, Ilham NOVA NOVITA Noveriera, Adinda Ayu Novia Restu Windayani, Novia Restu Noviana, Cahyani Nur Anggraini, Dian Eka Nur Khamidah Nurachmawati Meindah Sari Nurul Yaqin Nurwasilah, Nurwasilah Palupi, Hana Indi Tisyria Cipto Permatasari, Kiswati Rozi Pinatih, Januartha Putra Pranaya, Randy Hadyan Prasetya, Johan Danu Putri Listia, Cory Artania Putu Oky Ari Tania Putut Laksminto Emanuel R. Azizah Rachmawati, Anisa Nur Rahadian Zainul Rahma, Salsabila Rayhanah Nadzifiah Rebezov, Maksim Reisner Rismawaty, Ika Ruby Riana Asparini Safitri, Warini Ayu Salsabila, Elsa Salsabila, Puteri Sandra Miladyna Sanusi, Obed Nelson Satriyo Nugroho, Satriyo Shita, Shita Siti Hadijah Sofyana, Nuril Maulidah Sri Lestari Utami Sri Masyeni, Dewa Ayu Putri Sudharma, I Putu Bagus Arya Utami, Sri Lestari Viona Rindu Pristia Wahyuni Dyah Parmasari, Wahyuni Dyah Wijaya, Putu Listya Ayu Wike Herawaty Winata, Lionesya Sukma Windyantini, Hanna Yosephi, Valensa Yudistira, I Kadek Alam Yuniekawati, Yuyun Zahida, Farah Zulfan Febriawan Zulfi Zaskia Ramadhini