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Journal : Journal of Tropical Plant Protection

The Application of Beauveria bassiana and Lufenuron Could Reduce the Reproduction of Fruit Fly (Bactrocera carambolae) (Drew dan Hancock) (DIPTERA: TEPHRITIDAE) Mochammad Syamsul Hadi; Toto Himawan; Luqman Qurata Aini
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstractThis study was conducted to examine the effect of Beauveria bassiana and insect growth regulator lufenuron on the fecundity and reproductivity decline of fruit fly B. carambolae adults. The study was conducted at the Laboratory of Entomology, Department of Plant Pests and Diseases, Facultyof Agriculture, University of Brawijaya from March 2012 until December 2012. The density of B. bassiana spores used in this study was 108 spores/ml and the lufenuron concentrations used are 0.5, 1, and 1.5 ml/l. The results showed that the application of B. bassiana spores at density of 108 spores/ml combined with lufenuron at the concentration of 1.5 ml/l significantly reduced the fecundity, egg fertility and reproduction of B. carambolae up to 95.69%.Keywords: Beauveria bassiana, Bactrocera carambolae, Lufenuron.
The effect of PGPR (Plant Growth Promoting Rhizobacteria) Pseudomonas fluorescens and Bacillus subtilis On Leaf Mustard Plant (Brassica juncea L.) Infected by TuMV (Turnip Mosaic Virus) Bogi Diyansah; Luqman Qurata Aini; Tutung Hadiastono
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.861 KB)

Abstract

AbstractOne problem that causing leaf mustard yield loss is the infection of Turnip Mosaic Virus (TuMV). Thevirus causes mild mosaic leaf with vein clearing, blister, malformation and stunting. The use of Plant Growth Promoting Rhizobacteria (PGPR) such as Pseudomonas fluorescens and Bacillus subtilis is oneeffort that could be used to solve the problem. Through the mechanism of induced resistance, thesebacteria can elicit the defense signal in plant for the defence against pathogens. In this study the use of Pseudomonas fluorescens and Bacillus subtilis was performed to test their benefit on leaf mustard planthealth against TuMV infection. This study was conducted with a randomized block design (RBD) byusing 8 treatments and 4 replications. On the experiment of the effect of PGPR on the root length ofleaf mustard plants, the design used was a completely randomized design (CRD) with 4 treatments and4 replications. Leaf mustard plants inoculated with Pseudomonas fluorescens and Bacillus subtilis hadlonger roots than those without the inoculation of Pseudomonas fluorescens and Bacillus subtilis. Inaddition, Pseudomonas fluorescens and Bacillus subtilis was also able to reduce the incubation periodand disease intensity of TuMV on the leaf mustard plant. The activity of catalase enzyme and phenolcontent was elevated in the leaf of leaf mustard plant inoculated with PGPR. The results suggested thatcatalase and phenol production probably play a role in plant defense of leaf mustard against theinfection of TuMV.Key word : Mustard, Turnip Mosaic Virus (TuMV), PGPR
Leaf Extract of Mirabilis jalapa L. Induced Defense of Tomato Plant (Lycopersicum esculentum Mill.) Against Cucumber Mosaic Virus (CMV) Infection Dian Eka Kusumawati; Tutung Hadiastono; Luqman Qurata Aini
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.388 KB)

Abstract

AbstractInfection of Cucumber Mosaic Virus (CMV) could reduce the tomato production up to 50%depending on the age of the plant during infection and tomato varieties. Until now the controlefforts against CMV infection have been made, but no consistent effective method was available.Recently, new approach by increasing the plant health to defense against plant disease wasincreased. It can be stimulated by inoculation of microbes associated with roots or by application of plant extract such as Mirabilis jalapa. In this study, the effect of M. jalapa leaf extract in differentconcentration to develop control against CMV infection in tomato plants was conducted. The results showed that the application of M. jalapa leaf extract could inhibit the development of CMV diseasein tomato plants. The higher the concentration of M. jalapa leaf extract applied on tomato plant, thelower disease incidence as well as the longer incubation period of CMV disease in tomato plants.The applications of M. jalapa leaf extract elevated the activity of peroxidase and catalase enzymes.Thus, the inhibition of CMV disease development in tomato plant by application of M. jalapa leafextract may related to the induction of peroxidase and catalase activity in tomato plants.Keywords: M. jalapa, CMV, plant defense, leaf extract, peroxidase, catalase
Virulency of Bacillus subtilis and Trichoderma harzianum Against Sprout Disease Pythium spp. in Cucumber Plants Minal Maimanah; Restu Rizkyta Kusuma; Luqman Qurata Aini
Journal of Tropical Plant Protection Vol. 2 No. 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.1

Abstract

Sprout disease (damping off) is one of the important diseases that attack a variety of horticultural plants. Besides, sprouts disease can also attack seeds that have not germinated (preemergence damping-off). This study aimed to determine and compare the ability of B. subtilis and T. harzianum in controlling the pathogen Pythium spp. in cucumber plants. Phy01CK and Phy01IP isolates were use in the virulency test and inoculation methods of pathogens. The inhibition test between B. subtilis and T. harzianum against Pythium spp. was conducted in the in vitro test. This research was used a randomized block design with 13 treatments and three replications. The results of the antagonist's effect in vitro between T. harzianum and Pythium spp. showed the highest suppressing growth of Pythium spp. which is 70%. B. subtilis, B. subtilis + T. harzianum and fungicides each having a zone of inhibition of 23%, 32% and 46%. In the in vivo tests, the application of biological agents with 3 different times was not affecting the incidence of damping-off disease at 10 days after planting.
Potential of Indigenous Yeast as a Paraquat Biodegradation Agent in Sumberbrantas, Batu City, Indonesia Bosha, Mohammed; Abadi, Abdul Latief; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 3 No. 1 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.1.1

Abstract

Herbicides are a substance or chemical compound used to inhibit growth or kill plants. Herbicides are toxic to weeds or pests as well as cultivated plants. The impact of using the herbicide paraquat causes some microbes to become more resistant and has the potential to be used as a paraquat biodegradation agent. Indigenous yeast is one of the microbes that can be used as a biodegradation agent because it is resistant to the environment. For this reason, This research aimed to investigate the indigenous yeast isolated from the potato field in Sumberbrantas village, Batu City as a bioremediation agent for paraquat herbicide residues.The sampling was carried out in the potato field of Sumberbrantas, Batu City, which in routine maintenance uses a herbicide with active paraquat (1,1 dimethyl -4, 4-bipyridinium dichloride).The isolation results obtained 6 isolates of indigenous yeast which had the potential as biodegradation agents for paraquat. Treatment of yeast isolates MB 1, MB 3, MB 4, MB 6, MB 7, MB 8 at concentrations of 250, 500, 1,000 and 2,000 paraquat were able to survive with a growth percentage of 75-100%. At a concentration of 5,000 Isolated yeast decreased with a growth percentage of 50-75% and MB 1 isolate with 10,000 ppm concentration still survive with a growth percentage of 1-25%. The highest biodegradability ability of yeast was in MB 1. The identification of MB 1 isolates based on molecular characters have similarities in the basic structure of 98.89% with Geotrichum silvicola UFMG 354-228S isolates with accession number NG_0606221.1.
Exploration of Endophyte Bacteria as Bio-Agent of Fusarium sp. on Jack Bean (Canavalia ensiformis) Under In-Vitro Testing Rahmadani, Pradina; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 5 No. 2 (2024)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2024.005.2.3

Abstract

Indonesia is the tropical country that becomes the home of millions type of flora such as jack bean (Canavalia ensiformis). This plant has not gotten enough attention from locals as a beneficial commodity. The purpose of this research is to find out the potential of endophyte bacteria of jack bean as bio-agent to control the pathogenic attack on the jack bean caused by Fusarium sp. The step of research including sample collecting, isolation and identification of pathogenic fungus, isolation, and purification of endophyte bacteria from the root of jack bean plant, selection of endophyte bacteria as the antagonist of pathogenic fungus, inhibition test of endophyte bacteria against the fungus under in-vitro testing, and lastly, identification of bacteria. The result of the test used completely randomized design with 6 treatments and 4 replications. The result of research is there are 10 bacterial isolates. From the selection process, there are four isolates that are proven to inhibit Fusarium sp. as the fungal target, shown by the high percentage of fungal growth inhibition from antagonist test. At the end of the antagonist test, it was resulting in CE8 treatment as the highest percentage of fungal growth inhibition, reaching 76% in result. Characterization and identification were carried out to the 4 isolates, resulting in discovery of CE1 and CE5 coded bacterial isolates belong to Erwinia sp., and CE7 and CE8 belong to Clostridium sp.
Characterization of Plant Pathogenic Bacteria Causing Crown Rot Disease in Papaya (Carica papaya L.) Rania, Khansa Diva; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 6 No. 1 (2025)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2025.006.1.1

Abstract

Papaya (Carica papaya L.) is an annual plant belonging to the Caricaceae family. Papaya populations in Indonesia can be found in almost all regions. The extensive papaya cultivation areas in Indonesia often involve planting on wild land, which exposes papaya plants to various diseases. Crown rot disease is an example of a disease in papaya. Papaya plantations in Jajag, Gambiran, Banyuwangi Regency, are known to be affected by stem rot. To determine the appropriate disease control measures, it is necessary to identify the cause of the wet rot disease in papaya plant stems. The research was conducted through several stages, including sampling, media preparation, isolation of pathogenic bacteria, hypersensitivity tests, pathogenicity tests, potato soft rot tests, colony and cell morphological observation, Gram staining, oxidative/fermentative, yellow colony on YDC media, lecithinase, catalase, sensitivity to erythromycin, carbohydrate fermentation, and temperature treatment at 37°C and 40°C. The results showed that the plant pathogenic bacteria causing crown rot at the base of the stem in papaya plants were isolates B1 and B2, belonging to the group of Pantoea sp.; isolates B3, B6, and B7, belonging to the group of Erwinia sp.; and isolates B4 and B5, belonging to the group of Dickeya sp..
Co-Authors Abd Latif Abadi Abd Latif Abadi Abdul Cholil Abdul Cholil Abdul Karim Fanani Abdul Latief Abadi Abdul Latief Abadi Abdul Latief Abadi Achmad Noerkhaerin Putra Achmad Roekhan Adi Setiawan Agung Nugroho Agung Wahyu Susilo Aini Nurul Aini, Febrilia Nur Akhmad Rizali Albert Fernando Sitorus Alda Dwi Aprilia Alfi Inayati Aminudin Afandhi Aminudin Afandhi Anastasya, Nabilla Alya Andhy Handoko Andhy Handoko Anton Muhibuddin Apriyani, Aliffia Nuraita Ardiyan Taruna Arifah, Fitri Ariffin Noor Sugiharto Arifin Noor Sugiharto Aris Budiman Asnawi Asnawi Asparita, Yulia Dwi Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Bayu Setyawan, Bayu Berliana, Sonia Bogi Diyansah Bosha, Mohammed Cindy Diah Ayu Fitriana Cokorda Javandira Cokorda Javandira Daniel Happy Putra Dayanti, Ayu Ike Dewi, Fibrianti Shinta Dian Eka Kusumawati Dianti, Sylvia Dianti, Sylvia Herli Dinata, Gallyndra Fatkhu Diyana Lestari Dwi Wulandari Dyah Ayu Kusuma Wardani Eka Kartini Eriyanto Yusnawan Eriyanto Yusnawan Fibrianti Shinta Dewi Firdausi, Wita Galuh Setyanto Pradana Galuh Setyanto Pradana Ganestya Indina Sari Ganestya Indina Sari Hagus Tarno Hapsari, Femita Hasanah, Ifa Maulidah Husna Fikriya Baroroh I Made Indra Agastya Ichsan Firmansyah Imam Chanif Imam Chanif, Imam Indah Anita Sari Irisa Trianti Irisa Trianti Ismat Ishak Istiqomah Istiqomah Istiqomah, Istiqomah Ito Fernando Ito, Satoshi Iva Dewi Lestariningsih, Iva Dewi Izza, Junda Fauzul Karuniawan Puji Wicaksono Kestrilia Rega Prilianti Khoirun Enisa Maharina Khoirun Enisa Maharina Khoirunisa, Fenti Rahma Kusuma, Restu Rizkyta Liliek Sulistyowati Lilis Suryani Ma'alifah, Nur Maharina, Khoirun Enisa Minal Maimanah Mintarto Martosudiro Mochammad Syamsul Hadi Mochammad Syamsul Hadi Muhammad Akhid Syib’li Muhammad Akhid Syibli Muhammad Febriansyah Muhidin, Ahmad Nava Karina Nindi Kurniasari Novia Dwirani Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro Nur Azizah Nurul Aini Nurul Aini Nurul Aini Nurul Aini Oktaviani, Rahmania Pamungkas, Bayu Aji Paramyta Nila Permanasari, Paramyta Nila Prabowo, Matheus Randy Prillianti, Kestrillia Rega Puput Wahyuningsih Rachmi Masnilah Rahmadani, Pradina Rahman, Fakih Nur Ramadhan, Varotama Putra Ramadhani, Fatchuliani Safitri Rania, Khansa Diva Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rina Rachmawati Roekhan, Achmad Rudin, Sofyan Sudirman Saitama, Akbar Sallygresya Theodora Dwifelita Matondang Santoso, Verisca Agil Winanda Sektiono, Antok Wahyu Serdani, Army Dita Setyono Yudo Tyasmoro Sheila Desi Kharisma Sheila Desi Kharisma Silfiani, Intan Siska Irhamnawati Pulogu Soemarno Soemarno Suharjono Sukmarani, Elok Syamsuddin Djauhari Syamsul Arifin Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syibli, Muhammad Akhi Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Taruna, Ardiyan Tatiek Wardiyati Theresia Rani Kartika Ayu Tita Widjayanti Toto Himawan Toto Himawan Tri Ardiyati Tri Ardiyati Tri Ardyati Trisnawati, Luh Putu Adi Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Udayana, Cicik Uma Khumairoh Wardiyati, Tatiek Wita Firdausi Wiwik Jatnika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwit Wicaksono Jati Yani Kurniawan, Yani Yogo Setiawan Zainudin Zainudin Zainudin Zainudin Zainul Abidin