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Mahfut
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, Lampung 35144
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Journal : Journal of Multidisciplinary Applied Natural Science

 1 
Screening of Sugarcane Commercial GMP Varieties Tolerant to Drought Stress Based on Molecular Detection of P5CS Gene Mahfut Mahfut; Maulidya Ananda; Endah Susiyanti; Rochmah Agustina
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.233

Abstract

Sugarcane (Saccharum officinarum L.) requires sufficient a water supply to produce optimal productivity. The selection of the superior varieties of sugarcane that are tolerant to drought can be done by molecular detection of the presence of the pyrroline-5-carboxylate synthetase (P5CS) gene. The research is aimed at molecular detection of P5CS gene in a commercial varieties at PT Gunung Madu Plantations (GMP), Indonesia that are potentially drought resistant. Similar research has never been conducted at PT GMP, as evidenced by the absence of official publications. The varieties used are GMP 3, GMP 5, RGM 06-654, PS 864, RGM 08-1026, PSJT 941, RGM 01-1834, GP11, RGM 07-099, and RGM 02-108. The research phases include DNA isolation, quantitative and qualitative DNA amplification, and PCR product visualization. Molecular data analysis is based on DNA band scoring results in the form of binary data which is then used to calculate the level of polimorphism information content (PIC) on the primary data used. The results of the study showed that nine out of ten varieties had P5CS-specific band sizes of ±167 bp, namely GMP 3, GMP 5, RGM 06-654, PS 864, PSJT 941, RGM 01-1834, GP 11, RGM 07-099, and RGM 02-108. A total of four of the nine varieties showed P5CS gene band thicknesses of RGM06-654, PSJT 941, PS864, and RGM 01-1834 which indicates the selected varieties most resistant to drought. The result of the PIC calculation has a value of > 0.25 which indicates the primary P5CS used is quite informative. The molecular detection was continued on one selected variety is PSJT 941, which showed that the relationship analysis of P5CS gene shows close affinity with the isolates from China and Bogor.
Molecular Phylogeny of rDNA-ITS on Native Dendrobium in Lampung Mahfut, Mahfut; Syarif, Admi; Wahyuningsih, Sri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.263

Abstract

Dendrobium, a flagship collection of the Liwa Botanical Garden, is an endemic flora of the Southern Sumatra that requires preservation. One of the challenges in its conservation and potential development is the molecular identification. This molecular identification utilizes DNA barcoding with the rDNA-ITS marker as a practical, rapid, accurate, effective, and efficient alternative, complementing previous species-level identification results based on morphological characteristics. Amplification results from 5 selected samples showed specific bands measuring 300 bp. Sequence data analysis using BioEdit and MEGA V.11.0.11 software with 1000 bootstraps grouped all accessions into the same main cluster with a similarity range of 94–100%. Phylogenetic analysis revealed that accession D2 is similar to D. signatum from Japan (AB593662.1), and accession D3 to D. densiflorum (HQ114255), D4 to D. nobile (LC011413.1), accession D6 to D. trigonopus (KF143730.1), and accession D12 to D. faciferum (LC192955.1) from China. The results of this study will enrich the taxonomic and phylogenetic data of Dendrobium, which is essential for conservation and serves as a foundation for its development as a medicinal herbal plant.
Co-Authors Abda Abda Admi Syarif Agus Rahardi, Agus Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amanto Amanto Amnah, Alifah Zul Amrani, Alfina Anbiya, Lulu Anggi Anggreiny Apriyanti, Della Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asmanto, Budi Asriah Nurdini M. Asrini Puspitasari Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Chrisnawati , Lili Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fachry Abda El Rahman Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Febriansyah, Muhammad Fiska Humaizah Hajrah Hajrah, Hajrah Harfiani, Nabela H.N. Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Heri Satria Herlanda, Kishy Dhea Hermawan, Isna Ainun Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Kamisah Delilawati Pandiangan Kamisah Delilawati Pandiangan Kendari, Putri Kenedi, M Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Said Hasibuan Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muh. Khalifah Mustami Muhammad Irfan Ardiansyah Muslimin Muslimin Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Raden Mohamad Herdian Bhakti Rahmadani, Desti Deria Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Salsabila, Diana Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Sijabat, Veronica Elizabeth Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Syamsiar, Syamsiar Tamara, Derlian Ella Tengku Riza Zarzani N Treesya Panjaitan, Mitha Valentina Tri Joko Tripeni Handayani, Tundjung Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Umur, Khoirul Upreti, Brij Mohan Valentina Dwi Anggita Sari WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia, Yulia Rahma Syari Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri