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All Journal Jurnal Fitopatologi Indonesia Bioma : Berkala Ilmiah Biologi Bioscience Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Techno: Jurnal Penelitian Prosiding Seminar Biologi Bioeksperimen: Jurnal Penelitian Biologi Jurnal Florea Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Biodjati Jurnal SOLMA Jurnal Biologi Tropis JURNAL PENGABDI Jurnal Pertanian Agros Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi JURNAL PengaMAS Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Biota INSIST (International Series on Interdisciplinary Research) AGROSCRIPT Journal of Applied Agricultural Sciences Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Pelita Eksakta Konservasi Hayati Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Jaring SainTek International Journal of Engineering, Science and Information Technology Journal of Multidisciplinary Applied Natural Science TROPICAL GENETICS Journal of Sustainability Science and Technology JURNAL BIOLOGI PAPUA BULLET : Jurnal Multidisiplin Ilmu AMMA : Jurnal Pengabdian Masyarakat Seminar Nasional Teknologi dan Multidisiplin Ilmu Tropical Genetics BIO-SAINS | Jurnal Ilmiah Biologi Journal of Digital Market and Digital Currency Agroscript: Journal of Applied Agricultural Sciences Analit: Analytical And Environmental Chemistry

Mahfut

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, Lampung 35144

Author-ID : 6054974

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Social Sciences Transportation Other

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Stomata characters of sugarcane (Saccharum officinarum L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia Mahfut, Mahfut; Kendari, Putri; Syarif, Admi; Wahyuningsih, Sri; Susiyanti, Endah
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79860

The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding.

Identification and Efforts to Control Infection Odontoglossum Ringspot Virus (ORSV) on Orchid Mahfut, Mahfut
International Journal of Engineering, Science and Information Technology Vol 1, No 1 (2021)
Publisher : Malikussaleh University, Aceh, Indonesia

Odontoglossum ringspot virus (ORSV) is a orchid virus which infection most widely reported and has spread widely in the world, including in Indonesia. Viral infections can degrade the quality of the orchid plant so it is economically detrimental. To study a disease infection, identification is carried out first to determine the most appropriate method of control. These identification techniques include variations in infection symptoms, host range testing, testing of viral titers, observation of particle morphology, genome detection, and analysis of DNA and amino acid sequences. Efforts to control disease can be done through preventive and eradicative actions chemically, biologically, and based on artificial intelligence.

Polyploidy Induction of Rutaceae through Bio-catharanthine Treatment Aziz, Isna Rasdianah; Muthiadin, Cut; Hajrah, Hajrah; Alir, Rahmat Fajrin; Suryafly, Fanny Dhea; Amnah, Alifah Zul; Hermawan, Isna Ainun; Mustami, Muhammad Khalifah; Mahfut, Mahfut; Upreti, Brij Mohan
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.344

Indonesia has a tropical monsoon-type climate which suitable for agriculture and plantations. One of the main centers of orange fruits (Citrus sp.) production in Indonesia and become the top ten with the largest production is in South Sulawesi. Farmers commonly cultivate siam oranges (Citrus nobilis). Lime (Citrus aurantifolia) has not yet become the main production, which is inversely proportional to the high demand in South Sulawesi. This study aims to determine the ability of bio-catharanthine as a polyploidy agent against the phenotype of the familia Rutaceae cultivated in South Sulawesi. The research was carried out in Laboratory of Genetics and Molecular Biology, Department of Biology, Universitas Islam Negeri Alauddin Makassar using randomized complete block design consisted of two conditions (bio-catharanthine concentration including C1 = Bio-catharanthine 0.05%; C2 = Bio-catharanthine 0.075%; C3 = Bio-catharanthine 0.1% and immersion time including T1 = 3 h; T2 = 6 h). Phenotypic observations include plant height, the number of leaves and nodes, the length of roots, and the number of roots per shoot. The results of the study showed that the bio-catharanthine immersion of 0.05-0.075% for 6 hours in Rutaceae had a significant effect on their phenotypic characters.

Phylogenetic analysis in silico of orchid species Dendrobium based on rbcl marker Muhammad Febriansyah; Mahfut
Tropical Genetics Vol. 4 No. 2 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i2.68

Dendrobium is one of the genera of the third largest Orchidaceae family which reaches about 1184 species in the world. In Indonesia, this genus of orchids is a wealth of genetic resources that are abundant in the Eastern region, such as Papua and Maluku. These genetic resources have not been optimally utilized as elders in crossbreeding to produce offspring that have characteristics according to what consumers want. Due to the limitations of these morphological characteristics, another character is needed that can support the systematics of orchids, namely molecular characters. Plant kinship analysis can use molecular characters in the form of DNA strands that can overcome the weaknesses of morphological data that are known to have limitations and tend to be influenced by the environment. This study aims to analyze the kinship of several types of Dendrobium based on the rbcL gene and add to the basic data source of germplasm from the genus Dendrobium. The samples used were nucleotide sequences of 11 nucleotide sequences consisting of 10 types of Dendrobium (D. anosmum, D. jinghuanum, D. catenatum, D. nobile, D. jenkinsii, D. pahangense, D. nobile, D. pulchellum, D. fimbriatum, and D. pulchellum) which is used as an in group and 1 species of the genus Phalaenopsis namely Phalaenopsis equestris which is used as an outgroup. The results of Dendrobium kinship analysis with an in silico based rbcL gene molecular approach show that species in the same genus are not necessarily closely related. The species that is a member of the out group is Phalaenopsis equestris,. Phalaenopsis equestris forms its own tree branches, while the other 10 species are on the same phylogeny tree branches.

RESPONSE OF GREEN BEANS (Vigna radiata L.) GERMINATION ON LEAVES AND RHIZOMES COGONGRASS (Imperata cylindrica L.) ALLELOPATHY Sari, Heni Erlita; Mahfut, Mahfut
BIO-SAINS : Jurnal Ilmiah Biologi Vol 3 No 2 (2023): Bio-Sains Vol 3 No 2, Agustus 2023
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v3i1.3033

Cogongrass is a plant that contains allelochemicals, so it has the capability to reduce viability and early growth in green bean germination. This study aimed to determine the effect of allelopathy extract on the size and number of root cells in mung bean sprouts. The study uses the Completely Randomized Design with 1 treatment and 5 concentration levels of reed extract and repeated twice. The concentrations of the reeds used were 0% (control), 25%, 50%, 75%, and 100%. The results showed that high concentrations of Cogongrass allelopathic extract inhibited the growth of soybean sprouts based on plant length. Each concentration of reed extract did not show a significant difference in cell length or cell density of mung bean sprouts.

Screening of Sugarcane Commercial GMP Varieties Tolerant to Drought Stress Based on Molecular Detection of P5CS Gene Mahfut Mahfut; Maulidya Ananda; Endah Susiyanti; Rochmah Agustina
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.233

Sugarcane (Saccharum officinarum L.) requires sufficient a water supply to produce optimal productivity. The selection of the superior varieties of sugarcane that are tolerant to drought can be done by molecular detection of the presence of the pyrroline-5-carboxylate synthetase (P5CS) gene. The research is aimed at molecular detection of P5CS gene in a commercial varieties at PT Gunung Madu Plantations (GMP), Indonesia that are potentially drought resistant. Similar research has never been conducted at PT GMP, as evidenced by the absence of official publications. The varieties used are GMP 3, GMP 5, RGM 06-654, PS 864, RGM 08-1026, PSJT 941, RGM 01-1834, GP11, RGM 07-099, and RGM 02-108. The research phases include DNA isolation, quantitative and qualitative DNA amplification, and PCR product visualization. Molecular data analysis is based on DNA band scoring results in the form of binary data which is then used to calculate the level of polimorphism information content (PIC) on the primary data used. The results of the study showed that nine out of ten varieties had P5CS-specific band sizes of ±167 bp, namely GMP 3, GMP 5, RGM 06-654, PS 864, PSJT 941, RGM 01-1834, GP 11, RGM 07-099, and RGM 02-108. A total of four of the nine varieties showed P5CS gene band thicknesses of RGM06-654, PSJT 941, PS864, and RGM 01-1834 which indicates the selected varieties most resistant to drought. The result of the PIC calculation has a value of > 0.25 which indicates the primary P5CS used is quite informative. The molecular detection was continued on one selected variety is PSJT 941, which showed that the relationship analysis of P5CS gene shows close affinity with the isolates from China and Bogor.

Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia Mahfut, Mahfut; Sari, Mai; Akin, Hasriadi Mat; Wahyuningsih, Sri; Irawan, Bambang
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12563-75

Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.

Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia Mahfut, Mahfut; Daryono, Budi Setiadi; Somowiyarjo, Susamto
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Native orchid is one of Indonesian natural resources which play important role as parental materials in breeding program. Virus infection is one of the limiting factors in the cultivation of orchid. The purpose of this study was to detect Odontoglossum ringspot virus (ORSV) from native orchid. Symptomatic orchids were collected from 5 botanical gardens, i.e. Bogor, Cibodas, Purwodadi, Balikpapan, and Enrekang Botanical Gardens. Detection and identification was conducted by serological method using ORSV specific antisera, followed by RT-PCR and DNA sequencing. The serological test showed that 5 samples gave positive reaction against ORSV antiserum, i.e. Phalaenopsis amboinensis (KRB2) and Phalaenopsis amabilis (KRB12) from Bogor Botanical Garden, Phalaenopsis amabilis (KRP18) and Dendrobium salacence (KRP20) from Purwodadi Botanical Garden, dan Phalaenopsis modesta J. J. Sm. (KRBp5) from Balikpapan Botanical Garden. RT-PCR of the 5 samples using specific primer of ORSV coat protein gene was successfully amplified fragment DNA with size ± 474 bp. Homology analysis of those 5 ORSV isolates showed the highest index similiarity of 99.8% with corresponding sequences from 14 other ORSV isolates. Phylogenetic analysis indicated that ORSV KRB2 and KRP18 isolates was clustered in a separate group far from ORSV isolates in other countries. This is the first report of ORSV infection on native orchids collection from 5 botanical gardens in Indonesia.

Identifikasi Orchid Mycorrhiza Pada Akar Anggrek Dendrobium nobile Arsitalia, Metari; Wahyudi; Bambang Irawan; Mahfut
Florea : Jurnal Biologi dan Pembelajarannya Vol. 10 No. 2 (2023)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v10i2.21187

Orchid mycorrhiza is a type of fungus that is able to associate well with orchid plants. Orchids require fungal hyphae infection at every phase of their growth and development. Mycorrhizal fungi provide the organic and organic nutrients needed by orchid planting. Meanwhile, orchid plants are suppliers because the results of photosynthesis of orchid plants are distributed in the body of fungi as an energy source. Given the importance of the prospect of mycorrhizal utilization for orchids, it is necessary to continue to develop studies on identification. The purpose of this study is to isolate, identify and characterize mycorrhiza in Dendrobium nobile orchids. The method used was with a descriptive design on the identified orchids, samples in the form of Dendrobium nobile orchid roots, the media used was PDA media, transverse and longitudinal root cut types.Â The way it works includes sample preparation, media preparation, fungal isolation, fungal colony purification,

The Impact of Drought Stress on The Growth of Water Spinach (Ipomea Reptans Poir) Asadudin, David; Lulu Anbiya; Metari Arsitalia; Mahfut
Florea : Jurnal Biologi dan Pembelajarannya Vol. 11 No. 1 (2024)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v11i1.21188

Water spinach (Ipomoea reptans Poir) is a commonly cultivated vegetable in tropical regions like Indonesia. Water spinach faces challenges related to water availability, which can impact various cellular, biochemical, and physiological aspects. Drought can lead to reduced vegetative growth, and a lack of water can inhibit overall plant growth. Therefore, understanding the growth response of water spinach to water stress is crucial for achieving optimal results. This study used a Completely Randomized Design (CRD) with water concentration as the treatment. Water spinach seeds were selected before being sown and transferred to polybags. Irrigation was conducted with five levels of concentration: 100% (300 ml of water), 75% (225 ml of water), 50% (150 ml of water), 25% (75 ml of water), and 0% (0 ml of water). Observations were made at 14 days old with agronomic and anatomical parameters, and the data were analyzed using MANOVA followed by a Tukey post-hoc test at a 5% significance level with SPSS. The results showed that irrigation with 300 ml and 225 ml of water produced the highest number of stomata and the longest stomata size. For stomatal width, treatments of 300 ml, 225 ml, and 75 ml resulted in wider stomata. Meanwhile, for stomatal area, the 225 ml treatment showed the largest stomatal size. However, no significant differences were observed in plant height and leaf number among the treatments. Drought stress affects photosynthesis and plant growth, with increasingly severe impacts as drought intensity rises. Drought results in reduced stomatal number, smaller stomatal size, and inhibited plant growth.

Co-Authors Abda Abda Admi Syarif Agus Rahardi, Agus Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amanto Amanto Amnah, Alifah Zul Amrani, Alfina Anbiya, Lulu Anggi Anggreiny Apriyanti, Della Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asmanto, Budi Asriah Nurdini M. Asrini Puspitasari Aviva Indira Putri Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Chrisnawati , Lili Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fachry Abda El Rahman Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Fiska Humaizah Hajrah Hajrah, Hajrah Harfiani, Nabela H.N. Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Heri Satria Herlanda, Kishy Dhea Hermawan, Isna Ainun Ikhwan Ismail Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Istiqomah Kamisah Delilawati Pandiangan Kamisah Delilawati Pandiangan Kendari, Putri Kenedi, M Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Said Hasibuan M. Yogi Riyantama Isjoni Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muhammad Febriansyah Muhammad Irfan Ardiansyah Muhammad Khalifah Mustami Muslimin Muslimin Nisar Zaidal Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Raden Mohamad Herdian Bhakti Rahmadani, Desti Deria Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Salsabila, Diana Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Siswahyudianto Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukamto, Ika Sumiyarsi Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Syamsiar, Syamsiar Tengku Riza Zarzani N Tri Joko Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Umur, Khoirul Upreti, Brij Mohan Valentina Dwi Anggita Sari Veronica Elizabeth Sijabat WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia, Yulia Rahma Syari Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri

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