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All Journal Medula Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan PENA SAINS ZOO INDONESIA Indonesian Journal of Guidance and Counseling: Theory and Application Jurnal Penelitian Sains Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Medika Malahayati Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) JURNAL PENELITIAN PERAWAT PROFESIONAL BIO-EDU: Jurnal Pendidikan Biologi JK Unila (Jurnal Kedokteran Universitas Lampung) Zoo Indonesia Ruwa Jurai: Jurnal Kesehatan Lingkungan Hippocampus: Jurnal Pengabdian Kepada Masyarakat Journal of Pharmaceutical and Sciences. AMMA : Jurnal Pengabdian Masyarakat Berkala Ilmiah Biologi Bioma : Jurnal Biologi dan Pembelajaran Biologi JPM (Jurnal Pengabdian Masyarakat) Ruwa Jurai Berita Biologi Analit: Analytical And Environmental Chemistry
Endah Setyaningrum, Endah
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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PELATIHAN PEMBUATAN MINUMAN HERBAL UNTUK MENINGKATKAN IMUNITAS TUBUH STAFF KEBUN RAYA LIWA DI MASA PANDEMI COVID-19 Setyaningrum, Endah
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol. 6 No. 3 (2022)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jss.v6i3.375

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan motivasi dan pemahaman kepada staff UPT Kebun Raya Liwa (KRL) tentang pentingnya pemanfaatan herbal sebagai peningkat imunitas tubuh ditengah pandemi Covid-19. Sasaran kegiatan ini adalah staff UPT KRL yang mempunyai kemauan dan kemampuan untuk dilatih membuat minuman herbal. Pemilihan dan penetapan sasaran pelatihan ini dengan mempertimbangkan kondisi strategis peserta pelatihan yang berperan sebagai petugas lapangan di KRL. Staff lapangan KRL tersebut banyak berinteraksi dengan wisatawan pengunjung KRL yang datang dari berbagai daerah. Untuk meningkatkan imunitas tubuh dan mencegah penularan Covid-19, staff lapangan KRL ini membutuhkan suplemen supaya tubuh tetap fit dan imunitas tetap terjaga. Akhir akhir ini sering kali terjadi kelangkaan suplemen kesehatan di toko-toko obat dan apotek karena tingginya permintaan, sehingga diperlukan kemampuan mengolah minuman herbal untuk dijadikan suplemen. Dengan memiliki bekal kemampuan mengolah minuman herbal sendiri yang bahan bakunya mudah ditemukan di KRL dan sekitarnya, maka staff KRL akan dapat memenuhi kebutuhan suplemennya, dan jika sudah terampil dapat diproduksi sebagai oleh-oleh bagi wisatawan yang berkunjung ke KRL menjadi tambahan income KRL. Peserta Pelatihan pembuatan minuman herbal ini diikuti sebanyak 20 orang yang terdiri dari staff KRL, Pengelola Taman Kehati Lumbok dan Mahasiswa yang sedang melakukan Praktek Kerja di KRL. Metode yang digunakan dalam kegiatan pengabdian masyarakat ini adalah berupa penyampaian materi, tanya jawab, dilanjutkan dengan demontrasi pembuatan minuman herbal. Evaluasi aspek penyelenggaraan pelatihan dilakukan dengan pemberian kuisioner sebelum dan sesudah kegiatan berupa pre test dan postest. Hasil pelaksanaan Pelatihan menunjukkan bahwa terjadi peningkatan motivasi dan pemahaman peserta akan pentingnya fungsi herbal dan ketrampilan pembuatan minuman herbal, hal ini ditandai dengan antusiasnya peserta dalam diskusi baik sebelum maupun sesudah dilakukan demontrasi pembuatan minuman herbal.
THE EFFECTIVENESS OF Eucheuma cottonii EXTRACT AGAINST DENGUE VECTOR LARVAE AS AN EVALUATION FOR NATURAL LARVICIDE DEVELOPMENT Setyaningrum, Endah; Novrianda, Nabila Tias; Mumtazah, Dzul Fithria; Rosa, Emantis
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11236

Abstract

Dengue fever (DHF) is a tropical disease transmitted by the Aedes aegypti mosquito. Prolonged use of synthetic chemical larvicides such as Abate® has led to resistance and environmental impacts. Therefore, an alternative natural larvicide that is more environmentally sustainable is needed. Eucheuma cottonii is a red alga known to contain bioactive compounds such as flavonoids, alkaloids, terpenoids, saponins, and tannins that can potentially inhibit mosquito larvae development. This study aims to evaluate the effectiveness of the ethanol extract of E. cottonii on the mortality of Aedes aegypti instar III larvae as a basis for the development of natural biolarvicides. The study used a completely randomized design (CRD) with six treatments: four extract concentrations (50 ppm, 100 ppm, 200 ppm, and 300 ppm), one positive control (Abate®), and one negative control (water). Each test unit contained 20 third-instar larvae with four replicates. Observations were made 4, 8, 24, 48, and 72 hours after treatment. The highest larval mortality was achieved at a 200 ppm concentration of 13.25 ± 3.94. The LC₅₀ value at 24 hours was 42.35 ppm, while the LT₅₀ reached 52.77 hours, indicating a slow and less effective larvicidal effect at the test concentration. Although the ethanol extract of E. cottonii contains bioactive compounds, its effectiveness as a larvicide against Aedes aegypti larvae is still relatively low. These results indicate that E. cottonii is not optimally used alone as a bio-larvicide, but still has the potential to be further developed through improved extraction methods, increased concentrations, or combination formulations. This study provides a foundation for creating sustainable and environmentally friendly plant-based larvicides.
Identification of Active Compound and Effectiveness Test of 96% Tithonia diversifolia Ethanol Extract on Mortality of Aedes aegypti Mosquito A.P, Gadila; Mustofa, Syazili; Graharti, Risti; Setyaningrum, Endah
Medula Vol 16 No 1 (2026): Medula
Publisher : CV. Jasa Sukses Abadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53089/medula.v16i1.1758

Abstract

Dengue Hemorrhagic Fever (DHF) remains a major public health problem in tropical regions, with Asia accounting for 70% of global cases and Indonesia reporting a massive 90,269 cases in 2024. Vector control is the primary mitigation strategy due to limitations in available therapies and vaccines, yet reliance on chemical insecticides poses ecological toxicity and resistance risks, driving the need for safer bioinsecticide alternatives. Tithonia diversifolia is known to contain active phytochemical constituents with insecticidal potential. This true experimental study used a Post Test Only with Control Group design conducted from August to September 2025 involving 25 Aedes aegypti per group with four replications. Leaf simplicia were extracted using 96% ethanol, followed by alcohol-free verification, qualitative phytochemical screening, and formulation into spray preparations at concentrations of 5%, 10%, 15%, 20%, and 25%. Mortality was assessed over 24 hours, and statistical evaluation included univariate, bivariate, and probit analyses to determine LC50, LC90, LT50, and LT90. Extraction produced a 12.53% yield containing saponins, flavonoids, phenols, tannins, alkaloids, and steroids. Mosquito mortality increased with rising concentrations, with the highest effectiveness observed at 25%. The Kruskal–Wallis test confirmed significant differences between groups, while LC50 and LC90 values were 2.77% and 5.37%, respectively. Although the highest mortality was observed at 25%, the 20% concentration was considered optimal because it met WHO efficacy standards while using a lower extract concentration.
Efektivitas Beberapa Ekstrak Tanaman sebagai Ovisida terhadap Aedes aegypti dalam Pencegahan Demam Berdarah Dengue Septiani, Linda; aflika happy, terza; Graharti, Risti; Andrifianie, Femmy; Setyaningrum, Endah
Medula Vol 15 No 4 (2025): Medula
Publisher : CV. Jasa Sukses Abadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53089/medula.v15i4.1827

Abstract

Dengue Hemorrhagic Fever (DHF) remains a major public health concern, with Aedes aegypti serving as the primary vector. The continuous use of synthetic insecticides has led to resistance development and environmental concerns, highlighting the need for safer and sustainable alternatives. This review aims to evaluate the ovicidal potential of selected plant extracts against Aedes aegypti as an early-stage vector control strategy. A literature review was conducted using relevant national and international publications focusing on plant-based ovicides. The findings indicate that extracts from Piper nigrum L., Ocimum basilicum, Syzygium myrtifolium Walp., Tithonia diversifolia, Acorus calamus L., Solanum lycopersicum, and Euphorbia hirta L. effectively reduce egg hatchability of Aedes aegypti. The ovicidal activity is associated with secondary metabolites such as flavonoids, alkaloids, saponins, tannins, terpenoids, polyphenols, and essential oils, which disrupt egg membranes and inhibit embryonic development in a concentration-dependent manner. These findings suggest that plant extracts possess promising potential as natural ovicides and may serve as a basis for further pharmaceutical development and formulation in sustainable dengue vector control.
Antibacterial Activity Test of Methanol Extract of Gracilaria sp. against Escherichia coli Using Disc Diffusion Method: Uji Aktivitas Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli Dengan Metode Difusi Cakram Ajeng Pawesti, Ammanda Sadiva; Handayani, Kusuma; Setyaningrum, Endah; Busman, Hendri
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.9389

Abstract

The utilization of marine resources as an alternative source of natural antibacterial agents has been increasingly developed in recent years. Gracilaria sp., a type of red algae, is known to contain biologically active compounds with potential antibacterial properties, particularly against Escherichia coli. This study aims to determine the antibacterial potential of methanol extract from Gracilaria sp. against E. coli using an experimental method with a Completely Randomized Design (CRD). Data were analyzed using One-Way ANOVA followed by Tukey's test. The stock solution of the extract was prepared at a concentration of 100 mg/mL and tested at concentrations of 0.05%, 1%, 5%, and 10%, with ciprofloxacin as the positive control and sterile distilled water as the negative control. The experiment was conducted with three replications. The results showed that the methanol extract of Gracilaria sp. formed inhibition zones at the highest concentrations of 10% and 5%, with an average diameter of only 0.075 mm. Thus, the methanol extract of Gracilaria sp. exhibits weak antibacterial potential against Escherichia coli, as the inhibition zone formed was significantly smaller than that of the positive control, which reached 1.7 mm.
Co-Authors A.P, Gadila aflika happy, terza Ajeng Pawesti, Ammanda Sadiva Alfahri, Farhan Dzaki Alhafizoh, Fatimah Andrifianie, Femmy Annasya, Benazhir Saninah Arief Darmawan Arifiyanto, Achmad Asep Sukohar Aslina, Enna Athma, Dwi Aji Dwi Budi Santoso Farisi, Salman Gina Dania Pratami, Gina Dania Hadibrata, Exsa Halimi, Sofwan Happy, Terza Aflika Hardianto, Devina Hasan, Moh. Dwi Kurniawan Hendri Busman Hidayat Hidayat Idris, Mohamad Indarto Indarto Indri Windarti Indriyani Intan Kusumaningtyas Jausal, Annisa Nuraisa Jhons Fatriyadi Suwandi Juliasih, Ni Luh Gede Ratna Kanedi, M. Kanedi, Muhammad Kusuma Handayani, Kusuma L Widiastuti, Endang Lestari, Mutia Dinda Lidya Susanti, Nida Lili Chrisnawati, Lili Linirin Widiastuti, Endang Lusina, Septia Eva Marcellia, Selvi Masherni, Erniza Mayaguezz, Henky Muhammad Ilyas Mulya, Ireniza Pradevi Mumtazah, Dzul Fithria Novrianda, Nabila Tias Nukmal, Nismah Nuning Nurcahyani Pertiwi, Widya Ratna Primasari Pertiwi Priyambodo Priyambodo Puspita, Wana Putri, Sriana Qoyima, Allafia Ramdini, Dwi Aulia Reni Zuraida Risti Graharti Rosa, Emantis Sari, Galuh Retno Septiani, Linda Setiawan, Gigih SRW, Dyah Wulan Supriyo Supriyo Sutyarso Sutyarso Sutyarso Suwandi, Jhons Fatriayadi Syazili Mustofa Tugiyono Tugiyono Wahdah, Annisa Shohifatul Widiastuti, Endang Linirin Wiwi Febriani Wulandari, Kartika Dwi Yuandarru, Rhifana