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All Journal Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Althea Medical Journal Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication INDONESIAN JOURNAL OF PHARMACY Jurnal Sistem Kesehatan Journal of Medicine and Health Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies Indonesian Journal of Rheumatology Jurnal Pendidikan dan Pelayanan Kebidanan Indonesia (Indonesian Journal of Education and Midwifery Care) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Jurnal Ilmu Kefarmasian Indonesia Majalah Kedokteran Andalas Medika Kartika : Jurnal Kedokteran dan Kesehatan Health Information : Jurnal Penelitian Jurnal Aisyah : Jurnal Ilmu Kesehatan JK Unila (Jurnal Kedokteran Universitas Lampung) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Journal of Medicine and Health

Achadiyani

Departemen Anatomi, Fisiologi, Dan Biologi Seluler, Fakultas Kedokteran, Universitas Padjadjaran, Bandung

Author-ID : 632294

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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COMPARISON OF HISTOLOGICAL CHARACTERISTICS OF DRIED AND FRESH AMNION MEMBRANES AND DURA MATER IN NON-HUMAN PRIMATE (MACACA FASCICULARIS) Indah, Astrina Rosaria; Bolly, Hendrikus Masang B.; Faried, Ahmad; Arifin, Muhammad Zafrullah; Achadiyani, Achadiyani; Wirakusumah, Firman Fuad
Majalah Kedokteran Bandung Vol 51, No 1 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

This study aimed to characterize the histological properties of dry-lyophilized amniotic membrane, fresh amniotic membrane (AM), and duramater membrane in search for a biologically-derived material suitable for meninges surface reconstruction. This descriptive study was conducted at the Unit-3 Laboratory of Animal Test of PT. Bio Farma (Persero), Bandung and Cell Biology Laboratory of the Faculty of Medicine, Universitas Padjadjaran Bandung. This study was conducted from 2018-2019. Fresh Macacafascicularis placenta from healthy donors,classified as specific pathogen-free for TB, SIV, SV40, Polio type 1,2,3, Foamy virus and Herpes B virus, were obtained from selected caesarean sections.The harvested dried and fresh AM and duramater membrane were stained with hematoxylin-eosin and prepared for characterization. Histological examination of dry-lyophilized and fresh AM showed similar results. Histologically, AM is represented by a single layer of metabolically-active cuboidal to columnar epithelium with microvilli firmly attached to a basement membrane and an avascular and relatively sparsely populated stroma. Meninges layers consists of 3 layers: duramater, arachnoid membrane, and piamater. Most of these cells have the same characteristics as fibroblasts, including long organelles and nuclei with various levels of fibril formation. The histological study of amniotic membrane and duramater membrane shows comparable results. The AM is a biologically-derived material suitable for meninges surface reconstruction since its histological structure is somewhat similar to that of the duramater. Its structure is ideal for replacing duramater since it has several characteristics, such as having hygroscopic properties, good biocompatibility, relatively easy to apply, and inexpensive. Key words: Dried and fresh amnion membranes, duramater membranes, non-human primate Karakteristik Histologis Membran Amnion Jenis Kering dan Segar dengan Membran Duramater pada Primata Non-Human Macaca fascicularisPenelitian ini bertujuan mengetahui karakteristik histologis membran amnion kering yang diliofilisasi, membran amnion segar, dan duramater, dalam rangka mencari bahan biologis yang cocok untuk rekonstruksi permukaan meninges. Penelitian deskriptif dilakukan di Laboratorium Hewan Uji PT. Bio Farma dan Laboratorium Biologi Sel FK Universitas Padjadjaran periode 2018-2019. Plasenta Primata non-human Macaca fascicularis segar dari donor sehat, yang bebas dari pathogen spesifik TB, SIV, SV40, Polio tipe 1, 2, 3, virus Foamy dan virus Herpes B, diperoleh dari seksio sesarea. Kemudian, dilakukan pewarnaan dengan hematoxylin-eosin untuk membran amnion kering dan segar, serta membran duramater untuk mengetahui karakterisasi histologisnya. Pemeriksaan histologis membran amnion kering-yang aktif bermetabolisme hingga kolumnar dengan mikrovili; melekat kuat pada membran basal dan stroma yang avaskular dan relatif jarang. Lapisan Meninges terdiri dari 3 lapisan: duramater, arachnoid dan piamater. Sebagian besar sel-sel ini memiliki karakteristik yang sama dengan fibroblas. Studi histologis membran amnion dan membran duramater memiliki struktur yang relatif serupa. Membran amnion adalah material yang secara biologis cocok untuk rekonstruksi permukaan meningen, karena struktur histologinya agak mirip dengan duramater. Oleh karena itu secara struktur, membran amnion ideal untuk menggantikan duramater karena memiliki beberapa karakteristik seperti sifat higroskopis, biokompatibilitas baik, mudah diterapkan, dan murah.Kata kunci: Membran amnion segar dan kering, membran duramater, primate non-human

Indeks Prestasi Kumulatif (IPK) Tahap Sarjana dan Hasil Multidisciplinary Examination (MDE) sebagai Prediktor Kelulusan CBT UKMPPD pada Mahasiswa Fakultas Kedokteran Universitas Padjadjaran Periode 2015-2016 Pratiwi, Yuni Susanti; Susanah, Susi; Achadiyani, Achadiyani; Hilmanto, Dany
Jurnal Kedokteran Universitas Lampung Vol 1, No 2 (2016): JK UNILA
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jk unila.v1i2.1636

Uji Kompetensi Mahasiswa Program Profesi Dokter (UKMPPD) adalah uji kompetensi yang bersifat high-stake exam di tataran nasional sebagai salah satu upaya penjaminan mutu pendidikan tinggi bidang kesehatan sesuai dengan amanah UU No.12/2012. Kompetensi akan dicapai mahasiswa setelah melalui proses pembelajaran terstruktur berbasis kompetensi. Kurikulum, proses pembelajaran dan evaluasi pembelajaran yang sesuai dan berbasis kompetensi sejak tahap sarjana akan menjadi alur yang jelas untuk kelulusan mahasiswa pada UKMPPD. Penelitian ini dilakukan bertujuan untuk menganalisis apakah IPK tahap Sarjana dan skor MDE dapat menjadi prediktor kelulusan UKMPPD. Penelitian ini menggunakan rancangan deskriptif analitik dengan populasi adalah mahasiswa Fakultas Kedokteran Universitas Padjadjaran yang mengikuti UKMPPD periode Februari 2015 sampai dengan Agustus 2016 sebanyak 524 peserta. Analisis data menggunakan analisis uji korelasi (Pearson Correlation).Hasil penelitian diperoleh korelasi antara antara nilai IPK tahap Sarjana dengan skor CBT UKMPPD begitu juga dengan rata-rata skor MDE tahap Sarjana dengan skor CBT UKMPPD.Simpulan.Hal ini menunjukkan bahwa proses pembelajaran dan metode evaluasi pembelajaran berkaitan erat dengan kelulusan mahasiswa pada UKMPPD sebagai refleksi kompetensi mahasiswa. Hasil ini menunjukkan bahwa IPK dan rata-rata nilai MDE tahap sarjana dapat menjadi prediktor skor kelulusan CBT UKMPPD. Hal ini juga menunjukkan bahwa kelulusan mahasiswa pada UKMPPD merupakan suatu proses berkelanjutan dari tahap sarjana dan profesi. Penelitian selanjutnya pada aspek OSCEUKMPPD sebaiknya dilakukan untuk melengkapi analisis selanjutnya terkait hal ini. [JK Unila. 2016; 1(2)]Kata kunci:IPK, MDE, Skor CBT, UKMPPD

Nigella sativa Infusion as an Antioxidant Agent Against Gentamicin-Induced Kidney Damaged in Mice Hamsiah binti Halim; Achadiyani Achadiyani; Tjahjodjati Tjahjodjati
Althea Medical Journal Vol 1, No 2 (2014)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.903 KB)

Background: Gentamicin is one of the most common antibiotics related to nephrotoxicity. It has been proposed that the nephrotoxicity is associated with the generation of the reactive oxygen species. Thymoquinone, an active compound of Nigella sativa, shows to have an antioxidant property. The study aims to identify the possible nephroprotective action of Nigella sativa infusion against gentamicin-induced kidney damaged in mice.Methods:This experimental study was carried out in the Department of Cell Biology Laboratory, Universitas Padjadjaran, Bandung from 10th November 2012 to 14th December 2012. There were four groups, each consisting of 6 mice. Group I (control negative), group II (gentamicin 100 mg/kg), group III (3.9 mg Nigella sativa infusion+gentamicin 100mg/kg) and group IV (7.8 mg Nigella sativa infusion+gentamicin 100mg/kg). The kidneys were evaluated histopathologically by light microscope. The percentage average number of normal proximal tubules in group I and the percentage average number of proximal tubules damaged in group II, III and IV were measured.Results: The results showed the percentage average number of the proximal tubules damaged in group II, III and IV were 14.53%, 7.49% and 3.94% respectively. Significant differences were observed between group II and III, group II and IV, and group III and IV.Conclusion:Nigella sativa infusion protects against gentamicin-induced kidney damage in mice.Keywords: gentamicin, kidney, Nigella sativa infusion Infusa Nigella sativa sebagai Antioksidan Terhadap Kerusakan Ginjal padaTikus yang Diinduksi Gentamisin LatarBelakang: Gentamisin adalah salah satu antibiotik yang paling sering dihubungkan dengan nefrotoksisitas. Nefrotoksisitas yang terjadi berhubungan dengan pembentukan reactive oxygen species. Thymoquinone, senyawa aktif Nigella sativa, telah terbukti memiliki sifat antioksidan. Penelitian ini bertujuan untuk mengidentifikasi sifat nefroprotektif infusa Nigella sativa terhadap kerusakan ginjal pada tikus yang diinduksi gentamisin.Metode: Penelitian eksperimental ini dilakukan di Laboratorium Departemen Biologi Sel, Fakultas Kedokteran, Universitas Padjadjaran, Bandung dari 10 November 2012 –14 Desember 2012. Ada empat kelompok masing-masing terdiri atas 6 tikus. Kelompok I (control negatif), kelompok II (gentamisin 100 mg /kg), kelompok III (3,9 mg Nigella sativainfus + gentamisin 100mg/kg) dan kelompok IV (7,8 mg Nigella sativa infus + gentamisin 100mg/kg). Ginjal kemudian dievaluasi secara histopatologi dengan mikroskop cahaya. Persentase rata-rata jumlah tubulus proksimal yang normal dalam kelompok I dan persentase rata-rata jumlah tubulus proksimal rusak dalam kelompok II, III dan IV diukur.Hasil: Persentase rata-rata jumlah tubulus proksimal rusak dalam kelompok II, III dan IV adalah 14,53%, 7,49% dan 3,94%. Perbedaan signifikan yang diamati adalah pada kelompok II dengan III, kelompok II dengan IV, serta kelompok III dan IV.Simpulan: Infusa Nigella sativa infuse melindungi terhadap kerusakan ginjal yang diinduksi gentamisin pada tikus.Kata kunci: gentamisin, ginjal, infusa Nigella sativaDOI: 10.15850/amj.v1n2.354

Propolis of Trigona spp. Protects Mucosa from Aspirin-Induced Gastric Mucosal Damage in Rats Achadiyani Achadiyani; Anindita Laksmi; Dolvy Girawan
Althea Medical Journal Vol 7, No 1 (2020)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v7n1.1677

Background: Helicobacter pylori and non-steroid anti-inflammatory drugs are the major causes of peptic ulcer in the world. Indonesian native stingless bee species, Trigona spp., produces propolis that might be effective to protect mucosal damage. The aim of the study was to determine the protective effect of Trigona spp. propolis on aspirin-induced gastric mucosal damage in rats.Methods: This experimental study was conducted from September–November 2013 at Animal Laboratory of Department of Pharmacology and Therapy Faculty of Medicine Universitas Padjadjaran. Healthy male Wistar rats (n=24) aged 2–3 months old and weighed 200–250 grams were randomly divided into three groups. The first group was control negative, the second group was given 100 mg/kg body weight of aspirin, and the third group was given 200 mg/kg body weight of Trigona spp. propolis, one hour before administration of 100 mg/kg body weight of aspirin. After two weeks of treatment, rats were sacrificed by laparotomy to obtain gastric tissues, followed by processing for the paraffin section for histopathological analysis. The grade of gastric mucosal damage was determined under a light microscope. Data were then compared between groups using the Mann-Whitney test.Results: Oral administration of aspirin-induced gastric mucosal damage ranging from grade 0 to grade IV; whereas administration of propolis showed a reduction of gastric mucosal damage’s grade when compared to the aspirin group (p<0.05).Conclusions: Trigona spp. propolis has a protective effect on aspirin-induced gastric mucosal damage. Further study is encouraged to study an optimal dose of aspirin after propolis administration.

Hepatoprotective Effect of Trigona spp. Bee Propolis against Carbon Tetrachloride-Induced Liver Injury in Rat Rachel Amelia; Achadiyani Achadiyani; M. Begawan Bestari
Althea Medical Journal Vol 3, No 3 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4873.743 KB)

Background: Oxidative stress reaction can cause liver injury. This process can be prevented by antioxidant activities which can break the destructive chain caused by free radical substances in the liver. Propolis produced by Trigona spp. bee is known to have a high level of antioxidant. The aim of this study was to examine the effect of Trigona spp. bee propolis on liver histological toxicity caused by carbon tetrachloride-induced oxidative stress.Methods:This experimental study was conducted in September 2013 at the Animal Laboratory of Departement of Pharmacology and Therapy, Faculty of Medicine Universitas Padjadjaran. Twenty-four healthy male Wistar rats as objects were adapted for one week and randomly divided into 3 groups. Group I was the control negative, group II was given carbon tetrachloride on day 14, group III was given Trigona spp. bee propolis on day 1-14. On day 14, group III was injected CCl4 intraperitoneally. The quantitative data were statistically analyzed using the one way ANOVA and Tukey test with p value < 0.05.Results: Group I showed the liver contained normal cells, without significant injury of the membrane, round and complete nucleus. The average number of liver cell was 464 ± 9.59281 cells/field; group II underwent necrosis and the average of the cells was 146 ± 7.56885 cells/field; group III showed some normal liver cells, and some necrotic area with the normal liver cells average was 263 ± 14.10860 cells/field. The p-value=0.00.Conclusions: Trigona spp. bee propolis has a hepatoprotective effect against CCl4-induced liver injury histologically. [AMJ.2016;3(3):481–5]DOI: 10.15850/amj.v3n3.861

Snakebites and the Effect of Serum Anti Bisa Ular (SABU) Antivenom at Dr. Hasan Sadikin General Hospital Bandung, Indonesia: an Overview Period 2015–2019 Sharon Noor Alya; Enny Rohmawaty; Achadiyani Achadiyani; Muhammad Hasan Bashari; Hardisiswo Soedjana
Althea Medical Journal Vol 9, No 1 (2022)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v9n1.2392

Background: Snakebite is considered a global health issue, especially in the Southeast Asian region. However, data regarding snakebite cases in Indonesia are still very limited. This study aimed to explore the venomous snakebite cases and the serum anti bisa ular (SABU) antivenom effect in treating venomous snakebite caused by other than the snake species indicated in currently available SABU antivenom formulas.Methods: The analytical descriptive method with a cross-sectional study design was conducted in 2021 using a total sampling from the medical records of 63 patients diagnosed with snakebite at Dr. Hasan Sadikin General Hospital Bandung from 2015 to 2019. Characteristics of patients, clinical manifestation, and correlation between snakes species and outcomes after treatment with or without administration of SABU antivenom were collected.Results: Out of 63 patients, males (79%) were predominant with an average age of 39 years. Fifty-six patients arrived at the Emergency Room less than 24 hours after the occurrence (89%). Most cases were categorized as grade 2 (41.%) where the upper extremities were commonly bitten the body area (64%). Edema (83%) was the most common manifestation. There was no correlation between snake antivenom administered to a specific snake species and the stated outcome (p=0.053), meaning that SABU antivenom might be an effective alternative to treat more types of snakebites.Conclusions: Snakebites are most common in males, attack the upper extremities, categorized as grade 2 with edema. Administration of SABU antivenom provides the similar outcome compared to the group caused by the Javan spitting cobra, Banded krait, and Malayan pit viper.

Etiology of Allergic Contact Dermatitis based on Patch Test Dita Maulida Anggraini; Endang Sutedja; Achadiyani Achadiyani
Althea Medical Journal Vol 4, No 4 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Allergic contact dermatitis is a type of contact dermatitis, whose prevalence tends to increase every year. Patch test is an important diagnostic test to confirm the disease. The result of patch test can help doctors to perform appropriate treatment. The aim of the study was to identify the most frequent allergen causing allergic contact dermatitis based on patch test results.Methods: This was a descriptive retrospective study among patients who underwent patch test at the Dermatology and Venereology Clinic in Dr.Hasan Sadikin General Hospital Bandung, Indonesia from August 1st, 2014 to August 31st, 2015. The patch test result was obtained from the patient’s medical record and recapitulation data. The patient’s medical record would be collected based on inclusion and exclusion criteria. This study also collected demographic data of the patients, including age, sex, and occupation.The collected data were presented using tables.Results: Out of 45 patient’s medical records, 19 patients were male (42.2%) and 26 patients were female (57.8%). Nickel sulphate was the most frequent allergen with 17 sensitization. Thirteen out of 17 patients who were sensitized by nickel sulphate were females. A great majority of the patients were in the age group of 15–44 years. Most of the patients were students (42.2%). Allergic contact dermatitis (64.4%) was the main clinical diagnosis among the patients.Conclusions: The most frequent allergen causes allergic contact dermatitis is nickel sulphate.

Effect of Mangosteen Pericarp Extract on Gastric Mucosal Damage Induced by Aspirin Jansen Budiono; Achadiyani Achadiyani; Dolvy Girawan
Althea Medical Journal Vol 3, No 3 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.697 KB)

Background: Peptic ulcer disease is commonly found in the community. Nonsteroidal anti-inflammatory drug (NSAID) such as aspirin is one of the predisposing factors to ulcers. According to various studies, plant extracts have been shown to produce promising results in the treatment of peptic ulcers. Xanthone, the active substance contained in the mangosteen pericarp extract has been extensively studied for its role in various diseases. The present study was undertaken to identify the effect of mangosteen pericarp extract on gastric mucosal damage.Methods: This study was carried out from September−November 2014 in the Animal Laboratory of the Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung. Thirty two rats were randomly divided into 2 groups with different treatments and were induced by aspirin. Histologic studies were conducted to determine the score of gastric mucosal damage. The parameters used were Wattimena’s criteria for damage of gastric mucosa. Data was analyzed using Chi Square test and the outcome was measured in relative risk.Results: The study revealed a significant association between pretreatment with mangosteen pericarp extract and incidence of gastric mucosal damage induced by aspirin (p < 0.05). Calculation with a modified 2 × 2 table revealed a relative risk of 0.625.Conclusions: Rats pretreated with mangosteen pericarp extract exhibit less gastric mucosal damage, in experimentally aspirin-induced ulcer. [AMJ.2016;3(3):388–91] DOI: 10.15850/amj.v3n3.885

PEMBENTUKAN KADER KESEHATAN UNTUK MENINGKATKAN PENGETAHUAN DAN KEMAMPUAN MELAKUKAN DETEKSI DINI KANKER YANG SERING TERJADI PADA WANITA DI DESA SUKAMANAH DAN DESA CIHAURKUNING, KECAMATAN MALANGBONG KABUPATEN GARUT Dewi, A.R. -; Nurdiamah, E. -; Achadiyani -
Dharmakarya Vol 2, No 2 (2013): Dharmakarya
Publisher : Universitas Padjadjaran

Data Riset Kesehatan Dasar tahun 2007 menunjukkan bahwa wanita usia subur (WUS) rentan terhadapkematian akibat kanker leher rahim (serviks) dan kanker payudara. WHO menempatkan Indonesiasebagai negara dengan jumlah penderita kanker leher rahim terbanyak di dunia dan Kanker jenis iniadalah penyebab kematian nomor satu pada wanita di Indonesia. Setiap hari sekitar 20 wanita Indonesiameninggal karena kanker leher rahim. Saat ini, jumlah penderita kanker payudara di Indonesia terbanyakkedua setelah kanker leher rahim. Tingginya angka kematian dan angka kesakitan kanker leher rahim dankanker payudara di negara-negara berkembang termasuk Indonesia antara lain disebabkan terbatasnyaakses penapisan dini (screening) dan pengobatan. Hal lain yang menjadi penyebab adalah kurangnyapengetahuan masyarakat tentang gejala kanker dan gejala awal yang kadang juga sulit terdeteksi sehinggamayoritas penderita datang berobat dalam stadium lanjut. Oleh karena itu, kami memandang pentinguntuk melakukan kegiatan penyuluhan dan pelatihan untuk membentuk kader kesehatan. evaluasi yangdilakukan Dari hasil kuisioner yang dikumpulkan tersebut, diperoleh hasil bahwa terjadi peningkatanpengetahuan responden mengenai penyakit kanker payudara dan kanker leher rahim. Pengetahuan yangdimaksud meliputi jinak atau ganasnya, faktor resiko, gejala, cara pengobatan, kesembuhan penyakit,upaya deteksi dini, dan cara pencegahan kedua penyakit tersebut. Sebagai tindak lanjut kegiatan, kamimenyarankan agar penyuluhan mengenai kedua penyakit ini dan pelatihan SADARI dilakukan lagi ditingkat RT (Rukun Tetangga) agar semakin banyak masyarakat yang mengetahui. Selain itu, juga kerjasama dengan pihak puskesmas untuk melakukan tes IVA secara berkala sebagai upaya deteksi dinikanker leher rahim. Dengan kedua upaya ini, diharapkan angka kesakitan dan angka kematian akibatpenyakit kanker payudara dan kanker leher rahim dapat diturunkan.Kata kunci: Kanker leher rahim, dan payudara dan tingginya angka kematian, penyuluhan dan pelatihanSADARI-kader kesehatan

Pengaruh Krim Asam Traneksamat terhadap Pembentukan Keriput dan Kadar Matriks Metaloproteinase-1 pada Mencit (Mus Musculus) Jantan Galur Balb/c yang Dipajan Sinar Ultraviolet B Andrew Citrawan; Oki Suwarsa; Hendra Gunawan; Sudigdo Adi; Ronny Lesmana; Achadiyani Achadiyani; Sunaryati S. Adi
Indonesian Journal of Clinical Pharmacy Vol 8, No 2 (2019)
Publisher : Universitas Padjadjaran

Pemberian asam traneksamat (AT) per oral telah terbukti dapat mengurangi keriput yang diinduksi oleh kulit kering pada mencit. Namun, dosis oral yang setara terlalu besar untuk digunakan pada manusia dalam jangka panjang karena dapat menimbulkan toksisitas, sehingga perlu dicari sediaan alternatif lain secara topikal seperti sediaan krim. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian krim AT terhadap pembentukan keriput pada kulit mencit jantan galur Balb/c yang dipajan sinar ultraviolet B (UVB). Penelitian dilakukan terhadap 24 ekor mencit di Laboratorium Farmakologi dan Terapi serta Laboratorium Sentral Universitas Padjadjaran pada bulan Februari hingga Mei 2018. Mencit dibagi secara acak ke dalam empat kelompok, yaitu satu kelompok kontrol (P0) hanya mendapatkan pajanan sinar UVB tanpa krim AT dan tiga kelompok perlakuan (P1, P2, dan P3) mendapat pajanan sinar UVB dan diberikan krim AT dengan konsentrasi masing-masing 3%, 4%, dan 5%. Setelah 10 minggu, dilakukan penilaian kondisi keriput pada kulit punggung mencit berdasarkan metode Bisett, dilanjutkan dengan biopsi kulit punggung mencit untuk pemeriksaan kadar matriks metaloproteinase-1 (MMP-1) dengan teknik western blot (WB). Diperoleh perbedaan nilai rata-rata skor keriput yang bermakna sebesar 2,1±0,105 pada kelompok P0, 1,1±0,167 pada P1 dan P2, serta 1,3±0,211 pada P3 (p=0,005). Diperoleh pula nilai rata-rata kadar MMP-1 yang bermakna, yakni sebesar 0,75±0,08 pada kelompok P0, serta 0,54±0,033, 0,40±0,052, dan 0,54±0,072 pada P1, P2, dan P3 secara berturut-turut (p=0,008). Berdasarkan hasil tersebut, dapat disimpulkan bahwa pemberian krim AT mampu memperlambat pembentukan keriput dan menurunkan kadar MMP-1 pada kulit punggung mencit jantan galur Balb/c yang dipajan sinar UVB.Kata kunci: Keriput, krim asam traneksamat, matriks metaloproteinase-1, sinar ultraviolet B The Effect of Tranexamic Acid Cream on Wrinkle Formation and Matrix Metalloproteinase-1 Levels on Male Balb/c Mice Skin Exposed to Ultraviolet B RadiationsAbstractOral tranexamic acid (TXA) has been proven to ameliorate wrinkle induced by skin dryness in hairless mouse. However, the equal human oral dose is too high and can induce toxicity if used in long term, and study of topical preparations for wrinkle treatment is limited. Therefore, this study was conducted using topical preparations as an alternative for oral treatment to examine the effects of TXA cream in wrinkle formation. Four weeks old of twenty-four male Balb/c mice, divided into four groups, then 3%, 4% and 5% TXA cream were administered on the back skin of mice in each group shortly after ultraviolet B (UVB) exposure, except for control group that only exposed to UVB lights without given any TXA creams. Wrinkle formation and matrix metalloproteinase (MMP-1) level were observed after 10 weeks of treatments. There were significant differences of wrinkle score, with mean value were 2.1±0.105 for control group, 1.1±0.167 for 3% and 4% groups, and 1.3±0.211 for 5% group (p=0.005). There were also significant differences of MMP-1, with mean value were 0.75±0.08 for control group, 0.54±0.033, 0.40±0.052, and 0.54±0.072 for 3%, 4% and 5% group, respectively (p=0.008). Based on significant differences of wrinkle score and MMP-1 level, it can be concluded that administration of TXA cream may able to delay wrinkle formation and reduce the MMP-1 level on the mice skin exposed to ultraviolet B.Keywords: Matrix metalloproteinase-1, tranexamic acid, ultraviolet B, wrinkle

Co-Authors A.R. -, Dewi, A.R. Ahmad Faried Alfarafisa, Nayla Majeda Alfiah Rahmawati Amelia, Rachel Andrew Citrawan Andri Rezano Anggraini, Dita Maulida Anindita Laksmi Anita Deborah Anwar Ardella Tri Novianti Astrid F. Khairani Astrid Feinisa Khairani Bashari, Muhammad Hasan Cherry Azaria Cherry Azaria, Cherry Chou, Yoan Dany Hilmanto Deni K Sunjaya Dewi, A.R. - Dimas Erlangga Dini, Dini Dita Maulida Anggraini Dolvy Girawan E. -, Nurdiamah, E. Eko Fuji Ariyanto Endang Sutedja Endang Sutedja Endang Sutedja Endry Septiadi Enny Rohmawaty Farid Husin Firdaus, Muhammad Bintang Firman Fuad Wirakusumah Fitri Aulia Gaga Irawan Nugraha Hamsiah binti Halim Hamsiah binti Halim Hardisiswo Soedjana Hartati Purbo Dharmadji, Hartati Purbo Hendra Gunawan Hendrikus Masang Ban Bolly Herry Herman Ida Kinasih Ika Friscila Imelda, Imelda - Indah, Astrina Rosaria Insi Farisa Arya Insi Farisa Arya, Insi Farisa Irma Ruslina Ishak Abdulhak Ivanna Valentina Jansen Budiono Jansen Budiono Julaecha Julaecha Kamilah, Mutiara Mila Kartika Farahdilla Fitri Kurnia Wahyudi Laniyati Hamijoyo, Laniyati Latama, Zahra Nabila Lia Faridah Meita Dhamayanti Michael V.L Tumbol Mohammad Ghozali Monita Sugianto Muhammad Begawan Bestari Muhammad Zafrullah Arifin Nadifa, Safira Nanan Sekarwana Nani Murniati Nikla Sekar Salsabila Nur Atik Nur Ramadani Meliani Syukri Nurdiamah, E. - Oki Suwarsa Pitaloka, Pritha Pratiwi, Auria Ekanti Putri Halleyana Rahman Rachel Amelia Ramadhani Eka Putra Ratumanan, Samuel Permana Reni Farenia Reni Farenia Reni Farenia Rina Puspasari Herdiawan Rizqy Dimas Monica Ronny Lesmana Rovina Ruslami, Rovina Sadeli Masria Sari Puspa Dewi Savira Ekawardhani Sharon Noor Alya Shelly Iskandar Silvana Anggreini Rosa Sudigdo Adi Sudigdo Adi Sudigdo Adi Sunarjati Sudigdo Adi Sunaryati S. Adi Susi Susanah Tenny Putri Teresa Lucretia Teresa Lucretia, Teresa Tjahjodjati Tri Hanggono Achmad Tri Hanggono Achmad Tryando Bhatara Uci Ary Lantika Uni Gamayani Vita Murniati Tarawan Vitriana Vitriana Yuli Kurniawati Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yuniarti

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