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All Journal ODONTO Dental Journal Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association Jurnal Kreativitas PKM Dentin Jurnal Insan Farmasi Indonesia Odonto dental journal

Tri Putri, Deby Kania

Department Of Biomedic, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin

Author-ID : 682171

Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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Characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules Deby Kania Tri Putri; Indah Listiana Kriswandini; Muhammad Luthfi
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Dental caries is a major problem in oral cavity. If dental caries causes cavity, the structure of dental hard tissue will not be reversible because of damage in the structure of the hard tissue. The early pathogenesis mechanism of dental caries is an adhesion interaction between cariogenic Streptococcus mutans microorganisms and tooth surface pellicles. The attachment involves a specific molecular component interaction between the bacterial complement molecules and the surface of the host. Streptococcus sanguis as a dominant ecology at the beginning of bacterial plaque aggregation will colonize the tooth surface earlier than S. mutans. The surface of bacterial cells can express some adesin. The bacteria also can express receptors for adhesins of other bacteria. Specific receptors for adhesions of S. Mutans bacteria are not only found in the pellicles, but also present in pioneer bacteria, such as S. sanguis. Adhesion between those bacteria is called as coagregation. Purpose: This study aimed to analyze the characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules. Method: This study used a sonication method for protein isolation of S. mutans and S. sanguis bacterial biofilms, as well as electrophoresis method using 12 % SDS-PAGE gel and Western Blot analysis. Result: Results of the protein profile analysis of S. mutans biofilms using 12% SDS-PAGE showed that there were 17 bands, each of which molecular weights was 212, 140, 81, 65, 61, 48, 45, 44, 40, 39, 33 , 25, 23, 19, 17, 12, and 11 kDa. On the other hand, results of the protein profile analysis of S. sanguis biofilms using 12% SDS-PAGE showed that there were 15 bands, each of which molecular weight was 130, 85, 65, 61, 48, 46, 40, 37, 29, 25, 23, 21, 17, 15, and 12 kDa. And, results of the analysis of S. sanguis receptor molecules using Western blot showed that there were three bands, each of which molecular weight was 130, 85, and 40 kDa. Conclusion: S. sanguis bacteria have specific receptor molecules for S. mutans bacteria with the molecular weight of 130, 85, and 40 kDa.

Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract K. Khairiah; Irham Taufiqurrahman; Deby Kania Tri Putri
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

PENGARUH KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP JUMLAH KOLONI INTERAKSI Streptococcus sanguinis DAN Streptococcus mutans SECARA IN VITRO Vena Paramita Djunaidy; Deby Kania Tri Putri; R. Harry Dharmawan S
Dentin Vol 4, No 3 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Streptococcus sanguinis known as a pioneer bacteria of dental plaque formation. It has specific receptor for Streptococcus mutans adhesions to attach on tooth surface. S. mutans is the important cariogenic bacteria in the pathogenesis of dental caries. The dental caries risk can be prevented with chitosan in haruan fish scales. Chitosan contains an aminopolysaccharide which can inhibit bacterial colonization growth. Purpose: To find out effect of chitosan in haruan fish scales (Channa striata) to the total colony of interaction S. sanguinis and S. mutans in vitro. Methods: This study used experimental laboratories, post-test with control group design. Chitosan from haruan fish scales were fabricated by deproteination, demineralization, and deacetylation. The effect of chitosan in haruan fish scales test used Standard Plate Count (SPC). Results: The lowest average number colonies was shown in chitosan at 5% compared to chitosan at 0,25% and 2,5% concentration. Meanwhile, the highest average number colonies was shown in 1% acetic acid, namely 173,2 x CFU/mL. Conclusion: Chitosan of haruan fish scales (Channa striata) at 5% was the most effective concentration to inhibit the colonies growth of S. sanguinis and S. mutans interaction compared to total colonies at other concentrations, namely 60 x CFU/mL.Keywords: caries, chitosan, Streptococcus mutans, Streptococcus sanguinis ABSTRAKLatar Belakang: Streptococcus sanguinis dikenal sebagai bakteri pionir pembentukan plak gigi. S. sanguinis memiliki reseptor spesifik untuk adhesi Streptococcus mutans agar dapat melekat pada permukaan gigi. S. mutans merupakan bakteri kariogenik yang penting dalam patogenesis karies gigi. Risiko karies gigi dapat dicegah dengan kitosan sisik ikan haruan. Kitosan mengandung aminopolisakarida yang dapat menghambat pertumbuhan koloni bakteri. Tujuan: Untuk mengetahui pengaruh kitosan sisik ikan haruan (Channa striata) terhadap pertumbuhan jumlah koloni interaksi S. sanguinis dan S. mutans secara in vitro. Metode: Penelitian ini menggunakan experimental laboratories dengan desain post-test with control group. Pembuatan kitosan sisik ikan haruan dilakukan dengan deproteinasi, demineralisasi, dan deasetilasi. Uji pengaruh kitosan sisik ikan haruan dilakukan dengan Standard Plate Count (SPC). Hasil: Rata-rata jumlah koloni paling sedikit ditunjukkan oleh kitosan pada konsentrasi 5% dibandingkan dengan kitosan pada konsentrasi 0,25% dan 2,5%. Sementara itu, rata-rata jumlah koloni paling banyak ditunjukkan pada asam asetat 1%, yaitu 173,2 x CFU/mL. Kesimpulan: Kitosan sisik ikan haruan (Channa striata) pada konsentrasi 5% paling efektif dalam menghambat pertumbuhan koloni interaksi S. sanguinis dan S. mutans dibandingkan dengan jumlah koloni pada konsentrasi lainnya, yaitu 60 x CFU/mL.Kata kunci: karies, kitosan, Streptococcus mutans, Streptococcus sanguinis

PENGARUH APLIKASI SODIUM FLUORIDE 2% TERHADAP PH PLAK DAN PH SALIVA ANAK USIA 7-9 TAHUN Annisa Rahma; Nurdiana Dewi; Deby Kania Tri Putri
Dentin Vol 4, No 3 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Barito Kuala Regency has a high percentage of dental and oral health problems at 68.66%. This is due to its wetland areas with low pH water, people who use river water for daily necessities, and children's habits of consuming sweet foods that can cause caries. 2% Sodium Fluoride is an agent to prevent caries as it increases remineralization by converting hydroxyapatite to fluorapatite, so that fluoride can improve the pH. Objective: To determine the effect of 2% Sodium Fluoride application on plaque pH and salivary pH in children aged 7–9 years old. Method: This was a true experimental research with pretest-posttest control group design. This research consisted of 2 group, namely 2% Sodium Fluoride application group and the control group. Results: Based on paired t-test of plaque pH and salivary pH, there was a significant difference with values of p=0.017 and p=0.001 in the application group, whereas it showed no significant difference with values of p=0.167 and p=0.163 in the control group. Conclusion: There was an effect of 2% Sodium Fluoride application which was an increase of plaque pH and salivary pH in children aged 7–9 years old.Keywords: plaque pH, salivary pH, Sodium Fluoride, Wetland,ABSTRAKLatar belakang: Kabupaten Barito Kuala memiliki masalah kesehatan gigi dan mulut yang tinggi yaitu sebesar 68,66%. Hal ini disebabkan wilayahnya merupakan lahan basah yang memiliki air dengan pH rendah, masyarakat menggunakan air sungai untuk keperluan sehari-hari, dan kebiasaan anak-anak mengonsumsi makanan manis dapat menyebabkan karies gigi. Sodium Fluoride 2% adalah salah satu agen pencegahan karies karena dapat meningkatkan remineralisasi dengan cara mengubah hidroksiapatit menjadi fluorapatit sehingga fluor mampu meningkatkan pH. Tujuan: Mengetahui pengaruh aplikasi Sodium Fluoride 2% terhadap pH plak dan pH saliva anak usia 7-9 tahun. Metode: Penelitian ini menggunakan metode true experimental dengan rancangan pretest-posttest with control group design. Penelitian ini terdiri dari 2 kelompok, yaitu kelompok aplikasi Sodium Fluoride 2% dan kelompok kontrol. Hasil: Hasil uji paired t-test pH plak dan pH saliva pada kelompok aplikasi Sodium Fluoride 2% terdapat perbedaan bermakna dengan nilai p=0,017 dan p=0,001, sedangkan pH plak dan pH saliva pada kelompok kontrol tidak terdapat perbedaan bermakna dengan nilai p=0,167 dan p=0,163. Kesimpulan: Terdapat pengaruh aplikasi Sodium Fluoride 2% berupa peningkatan pH plak dan pH saliva anak usia 7-9 tahun. Kata kunci: lahan basah, pH plak, pH saliva, Sodium Fluoride

PENGARUH PERENDAMAN LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN KALSIUM PADA GIGI Muhammad Alfi Ghifari; Deby Kania Tri Putri; Yuniar Elsa Dwinuria
Dentin Vol 4, No 3 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Apart from being antibacterial, chitosan (Channa striata) fish scales solution can also be used to maintain tooth structure by reducing the solubility rate of hydroxyapatite or preventing demineralization of teeth in acidic conditions. Objective: To examine the effect of immersion of chitosan solution of scaled fish (Channa striata) 2,5% and 5% on the release of calcium (Ca) teeth. Method: Experimental study with a post-test only with control group design consisting of 3 treatment groups, namely negative control, a concentration of 2,5%, and 5% where each treatment after teeth were immersed in a solution of lactic acid with a pH 5,2 by reading the value of the release of tooth calcium released using atomic absorption spectrophotometry (AAS). Results: The concentrations of 2,5% and 5% were able to inhibit the release of calcium in the teeth. Conclusion: Chitosan scales chitosan (Channa striata) solution at a concentration of 2,5% has the best potential as a biomaterial in inhibiting demineralization. Keywords: Acid, Calcium, Chitosan, Demineralization, Haruan. ABSTRAKLatar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) selain bersifat sebagai antibakteri juga dapat digunakan untuk mempertahankan struktur gigi dengan mengurangi kecepatan kelarutan hidroksiapatit atau mencegah demineralisasi gigi dalam kondisi asam. Tujuan: Menguji pengaruh perendaman larutan kitosan sisik ikan haruan (Channa striata) 2,5% dan 5% terhadap pelepasan kalsium (Ca) gigi. Metode: Metode eksperimental murni dengan post test-only with control group design terdiri dari 3 kelompok perlakuan yaitu kontrol negatif, larutan kitosan sisik ikan haruan konsentrasi 2,5%, dan 5% yang diberikan pada gigi yang direndam pada larutan asam laktat dengan pH 5,2 dengan pembacaan kadar pelepasan kalsium gigi yang terlepas menggunakan alat spektrofotometri serapan atom (SSA). Hasil: Pada konsentrasi 2,5% sudah mampu menghambat pelepasan kalsium pada gigi. Kesimpulan : Larutan kitosan sisik ikan haruan (Channa striata) konsentrasi 2,5% dan 5% berpotensi sebagai biomaterial dalam menghambat demineralisasi pada gigi.Kata kunci : Asam, Demineralisasi, Haruan, Kalsium, Kitosan.

THE ANTIBACTERIAL EFFECT OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus mutans Naila Khairiyah; Didit Aspriyanto; Deby Kania Tri Putri
Dentin Vol 3, No 3 (2019)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Dental caries is a common problem of teeth and mouth. Dental caries is caused by a complex biological interaction between acidogenic bacteria, fermented carbohydrates and host factors, such as teeth and saliva. Streptococcus mutans is known as one of the main bacteria that causes dental caries. Kasturi leaf extract (Mangifera casturi) has secondary metabolite compounds, such as alkaloids, terpenoids, flavonoids, phenolics and saponins which are efficacious as antibacterial. Objective: The aim of this study was to analyzing the effectiveness of antibacterial kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus mutans bacteria. Method: This study was true experimental research with randomized pretest-posttest with control group design with 8 treatment groups consisted of kasturi leaves extract (concentration with 20 mg/ml, 25 mg/ml, 30 mg/ml, 35 mg/ml, 40 mg/ml and 45 mg/ml), positive control (chlorhexidine 0.2%) and negative control (aquades). Each treatment was repeated three times. Testing was done by liquid dilution method to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The data were analyzed using One Way Anova 95% (α=0.05) followed by Post hoc LSD for MIC data and Dunnet T3 for MBC data. Results: Based on the results of the analysis, it was known that Kasturi leaf extract had antibacterial effectiveness against the growth of Streptococcus mutans bacteria with MIC obtained at a concentration of 20 mg/ml and MBC obtained at 40 mg/ml. Conclusion: Kasturi leaf extract has an antibacterial effect against the growth of Streptococcus mutans. Key words: Antibacterial, kasturi leaf extract (Mangifera casturi), MIC, MBC, Streptococcus mutans

DAYA HAMBAT EKSTRAK BAWANG DAYAK (Eleutherine palmifolia (L.) Merr.) TERHADAP PERTUMBUHAN BAKTERI Lactobacillus acidophilus Nadalia Malika Bilqis; Isyana Erlita; Deby Kania Tri Putri
Dentin Vol 2, No 1 (2018)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Lactobacillus acidophilus is bacteria which causes the advanced caries if it is ignored which will infect another tissue. Bawang dayak is a plant that is used as a traditional medicine which can inhibit the Lactobacillus acidophilus because it has compounds such as flavonoid, alkaloid, glycoside, phenolic, quinones, steroid, essential oils, and tannin. Purpose: The purpose of conducting this study is to find out the resistivity zone from Lactobacillus acidophilus after giving the bawang dayak extract of various concentrations. Method: This study applies a true experimental with posttest only with control group design with six treatment groups which are bawang dayak extract with the concentration of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml, K(-) aquadest, and K(+) 2% of chlorhexidine digluconate. Maceration method is used to extract bawang dayak while diffusion method is used to test the resistivity and to measure the resistivity zone. Result: The result of the test that shows bawang dayak extract with the concentration of 20 mg/ml, 40 mg/ml, 60 mg/ml, and 80 mg/ml are obtained the average number of resistivity zone to 9,36 mm, 11,45 mm, 14,47 mm, 20,30 mm, and chlorhexidine digluconateis 15,33 mm. The data analysis of one-wayAnova and post-hoc LSD are obtained at (p<0.05) of bawang dayak extract which means that there is meaningful different to each treatment group. Conclusion: The resistivity zone of bawang dayak extract is higher than the positive control group which is 2% of chlorhexidine digluconate towards the growth of Lactobacillus acidophilus. ABSTRAKLatar belakang: Lactobacillus acidophilus merupakan bakteri pencetus karies lanjut yang apabila dibiarkan akan menginfeksi jaringan lainnya. Bawang dayak (Eleutherine palmifolia (L.) Merr.) merupakan tanaman yang digunakan sebagai obat tradisional yang dapat menghambat Lactobacillus acidophilus dikarenakan memiliki senyawa flavonoid, alkaloid, glikosida, fenolik, kuinon, steroid, minyak atsiri dan tannin. Tujuan: Mengetahui zona hambat dari bakteri Lactobacillus acidophilus setelah diberikan ekstrak bawang dayak dengan berbagai konsentrasi. Metode: Penelitian ini merupakan penelitian true experimental dengan post test only with control group design dengan 6 kelompok perlakuan, yaitu ekstrak bawang dayak konsentrasi 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml, kontrol K(-) aquadest dan K(+) klorheksidin diglukonat 2%. Metode maserasi digunakan untuk mengekstraksi bawang dayak sedangkan uji daya hambat menggunakan metode difusi dan pengukuran zona hambat. Hasil: Hasil dari pengujian menunjukkan bahwa ekstrak bawang dengan konsentrasi 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml didapatkan rerata zona hambat 9,36 mm, 11,45 mm, 14,47 mm, 20,30 mm, dan klorheksidin diglukonat sebesar 15,33 mm. Analisis data One Way Anova diperoleh

PENGARUH PERENDAMAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP STRUKTUR EMAIL GIGI Duhan Kanzu Balad; Deby Kania Tri Putri; Beta Widya Oktiani
Dentin Vol 5, No 2 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Acidic water from South Borneo peat land is one of the causes of enamel structure damage. Haruan fish (Channa striata) have scales that can be processed into chitosan. Chitosan contains amino and hydroxyl groups react with acid ions, increasing the environment pH and interfering the demineralization process. Purpose: To determine immersion effect of haruan fish scales chitosan on tooth enamel structure. Method: The research design was true experimental post test only with control group design. The treatment consisted of 4 groups, group I was the baseline, group II was the negative control group of teeth immersed in phosphoric acid 5.2 pH, groups III and IV were groups of teeth soaked with phosphoric acid 5.2 pH and chitosan solution with a concentration 2.5% and 5%. Data results were in the form of images from the buccal enamel surface observed with Scanning Electron Microscope. Results: Group III with 2.5% concentration of chitosan solution had less damage to enamel surface than negative control, and group IV with solution 5% chitosan concentration showed no damage and had better visualization than baseline group. Conclusion: Haruan fish scales chitosan has been shown to inhibit tooth enamel demineralization and play role in tooth remineralization process. Keywords : Chitosan, Demineralization, Enamel, Haruan Fish Scales, SEM. ABSTRAKLatar Belakang: Air dari lahan rawa gambut Kalimantan Selatan yang bersifat asam merupakan salah satu penyebab kerusakan struktur email gigi. Ikan haruan (Channa striata) memiliki sisik yang dapat diolah menjadi kitosan. Kitosan mengandung gugus amino dan hidroksil bereaksi dengan ion-ion asam, meningkatkan pH lingkungan serta menghambat proses demineralisasi. Tujuan: Untuk mengetahui pengaruh perendaman kitosan sisik ikan haruan terhadap struktur email gigi. Metode: Desain penelitian true experimental post test only with control group design. Perlakuan terdiri dari 4 kelompok, kelompok I sebagai baseline, kelompok II adalah kelompok gigi yang direndam dengan asam fosfat 5,2 pH sebagai kontrol negatif, kelompok III dan IV adalah kelompok gigi yang direndam dengan asam fosfat 5,2 pH dan larutan kitosan dengan konsentrasi 2,5% dan 5% dan data berupa gambar dari hasil pengamatan permukaan email pada bagian bukal dengan Scanning Electron Microscope. Hasil: Kelompok III dengan larutan kitosan konsentrasi 2,5% memiliki kerusakan permukaan email lebih minimal daripada kontrol negatif, dan kelompok IV dengan larutan kitosan konsentrasi 5% tidak menunjukkan kerusakan dan memiliki visualisasi yang lebih baik daripada kelompok baseline. Kesimpulan: Kitosan sisik ikan Haruan telah terbukti dapat menghambat demineralisasi email gigi dan berperan dalam proses remineralisasi gigi. Kata kunci : Demineralisasi, Email, Kitosan, SEM, Sisik Ikan Haruan.

DAYA HAMBAT EKSTRAK UBI BAWANG DAYAK (Eleutherine palmifolia (L.) Merr) TERHADAP PERTUMBUHAN Streptococcus mutans (Studi In Vitro Dengan Metode Difusi) Azilita Ananda; Deby Kania Tri Putri; Sherli Diana
Dentin Vol 2, No 1 (2018)
Publisher : Universitas Lambung Mangkurat

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ABSTRAKLatar belakang: Karies merupakan penyakit kronis jaringan keras gigi yang salah satunya disebabkan oleh faktor mikroorganisme yaitu bakteri Streptococcus mutans, pertumbuhan bakteri ini dapat dihambat dengan memberikan ekstrak umbi bawang dayak. Umbi bawang dayak merupakan tumbuhan herbal khas Kalimantan yang berpotensi sebagai alternatif obat kumur. Ekstrak umbi bawang dayak memiliki kandungan yang bersifat antibakteri salah satunya adalah fenol sebagai kandungan terbesar dengan konsentrasi 34,20% yang dapat merusak sel bakteri sehingga pertumbuhan Streptococcus mutans menurun dan lisis. Tujuan: Untuk mengetahui perbedaan daya hambat ekstrak umbi bawang dayak konsentrasi 80mg/ml dengan kontrol positif klorheksidin glukonat 0,2% terhadap pertumbuhan Streptococcus mutans. Metode dan bahan: Rancangan penelitian ini adalah true experimental design dengan post test only with control group. Penelitian ini menggunakan 6 kelompok perlakuan menggunakan sampel bawang dayak dengan metode maserasi dan pengujian aktivitas antibakteri dengan metode difusi. Hasil penelitian: Nilai rata-rata zona hambat ekstrak umbi bawang dayak konsentrasi 20mg/ml sebesar 11,59 mm, konsentrasi 40mg/ml sebesar 14,39 mm, konsentrasi 60mg/ml sebesar 18,53 mm, konsentrasi 80mg/ml sebesar 23,55 mm, kontrol positif klorheksidin glukonat 0,2% sebesar 21,39. Uji one-way Anova dan uji Post Hoc LSD menunjukkan bahwa terdapat perbedaan yang signifikan antara setiap kelompok perlakuan. Kesimpulan: Berdasarkan penelitian ini dapat disimpulkan bahwa terdapat perbedaan daya hambat ekstrak umbi bawang dayak konsentrasi 80mg/ml terhadap pertumbuhan Streptococcus mutans dengan zona hambat sebesar 23,55 mm dan klorheksidin glukonat 0,2% yang hanya memiliki zona hambat sebesar 21,39 mm. ABSTRACTBackground: Caries is a chronical disease of hard teeth tissue. It is caused by microorganism factor which is Streptococcus mutans bacterium, this bacterai can be inhibited with umbi bawang dayak extract . Umbi bawang dayak is Borneo particular herbal plant which has potential as an alternative to mouthwash. Umbi bawang dayak extracts contain antibacterial which have phenol as the largest content with 34.20% concentration. Purpose: To figure out the resistivity effect of umbi bawang Dayak extract with 20mg/ml, 40mg/ml, 60mg/ml and 80mg/ml concentration towards the growth of Streptococcus mutans. Method and Materials: This study applies a true experimental design with posttest-only with control group. This study takes six groups with 1 kg sampel of umbi bawang dayak using maserasi method and isolate of Streptococcus mutans using diffusion method. The Result of Research: The average number of inhibition zone of umbi bawang dayak extract with 20mg/ml concentration is 11.59mm, 40mg/ml concentration is 14.39mm, 60mg/ml concentration is 18.53mm, 80mg/ml concentration is 23.55mm. The average number of inhibition zone of umbi bawang dayak of chlorhexidine gluconate 0,2% is 21.39, and aquadest is 0.00mm. One-way Anova and Post-Hoc LSD show that there is significant difference between each of the treatment groups. Conclusion: Based on the result of the research, it can be concluded that there is different inhibition effect of umbi bawang dayak extract in 80mg/ml concentration with inhibition zone 23,55 mm and chlorhexidine gluconate 0,2% with inhibition zone 21,39 mm towards the growth of Streptococcus mutans.

EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN KASTURI (Mangifera casturi) TERHADAP PERTUMBUHAN BAKTERI Porphyromonas gingivalis Siti Khairiah; Beta Widya Oktiani; Deby Kania Tri Putri
Dentin Vol 4, No 3 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: The most often disease that occurs in the oral cavity is Periodontitis and one of that is chronic periodontitis type. The dominant bacteria in chronic periodontitis with 96,2% of prevalence is Porphyromonas gingivalis. Supporting treatment for chronic periodontitis is mouthwash administration, but in long term use may causing some side effect, so that an alternative antibacterial agent with minimal side effects is needed. One of the plants that have antibacterial compounds is the Kasturi plant typical of South Kalimantan, because contain tannins, terpenoids, flavonoids and phenolics. Purpose: Determine and analyzing the antibacterial effectiveness of Kasturi leaf extract (Mangifera casturi) against the growth of Porphyromonas gingivalis bacteria. Methods: This study was used True Experimental with posttest only control group and used the broth dilution method to determine MIC and agar dilution to determine the MBC. Results: The leaves extract of Kasturi has a Minimum Inhibitory Concentration at a concentration of 20 mg/ml and a Minimum Bacteriocidal Concentration at a concentration of 40 mg/ml. Conclusion: The leaves extract of Kasturi (Mangifera casturi) can inhibit and have antibacterial power against the growth of Porphyromonas gingivalis bacteria.Keyword : Chronic Periodontitis, Kasturi leaf extract, MIC, MBC, Porphyromonas gingivalisABSTRAKLatar Belakang: Penyakit yang sering terjadi di rongga mulut yaitu Periodontitis dan salah satu jenisnya yaitu periodontitis kronis. Bakteri dominan pada periodontitis kronis dengan prevalensi 96,2% adalah Porphyromonas gingivalis. Perawatan penunjang periodontitis kronis yaitu pemberian obat kumur, tetapi pada pemakaian jangka panjang menyebabkan efek samping sehingga diperlukan alternatif bahan antibakteri yang memiliki efek samping minimal. Salah satu tumbuhan yang bersifat antibakteri yaitu Tumbuhan Kasturi khas Kalimantan Selatan karena mengandung tanin, terpenoid, flavonoid dan fenolat Tujuan: Mengetahui dan menganalisis efektivitas antibakteri ekstrak daun kasturi (Mangifera casturi) terhadap pertumbuhan bakteri Porphyromonas gingivalis. Metode: True Experimental dengan rancangan posttest only with control group. Metode uji antibakteri yang digunakan yaitu dilusi cair untuk mengetahui KHM dan dilusi padat untuk mengetahui KBM. Hasil: Ekstrak daun kasturi memiliki Kadar Hambat Minimum pada konsentrasi 20 mg/ml dan Kadar Bunuh Minimum pada konsentrasi 40 mg/ml. Kesimpulan: Ekstrak daun Kasturi (Mangifera casturi) mampu menghambat dan memiliki daya antibakteri terhadap pertumbuhan bakteri Porphyromonas gingivalis.Kata kunci: Ekstrak Daun Kasturi, KBM, KHM, Periodontitis Kronis, Porphyromonas gingivalis

Co-Authors Agung Satria Wardhana Aizar Agi Syahrial Aizar Agi Syahrial, Aizar Agi Amalia Putri, Amalia Amelia, Aura Amy Nindia Carabelly Annisa Rahma Ariyati Retno Pratiwi, Ariyati Retno Aspriyanto, Didit Aulia Azizah Aulia Rahimah Azilita Ananda Azwar Fida Maulana Bambang Setiawan Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Budipramana, Melissa Candra Candra Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Diana Wibowo Duhan Kanzu Balad Eny Febriyanti Erika Norfitriah Fariz Asyraq, Muhammad Hafly Fatimah Maulideya Fatma Kirana Ferdy Juliannor Fajar Firdaus, I Wayan Arya Krishnawan Fitriyana, Annisa Haliza, Ninda Andrea Hamdani, Riky Hatta, Isnur Hutami, Wijayanti Diah Wasi Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indah Listiana Kriswandini Jamilatun Nisa Juli Harnida Purwaningayu K. Khairiah Kamila, Syifa Maharani Laillyza Apriasari Maulana, Noval Ihza Melati Ainun Nisa Melisa Budipramana, Melisa Moch. Andhika Firdausi Rachfa Muhammad Alfi Ghifari Muhammad Irfan Fanshuri Muhammad Luthfi Muhammad Luthfi MUHAMMAD RAYHAN Muhammad Zainal Fikri Nadalia Malika Bilqis Nadiya Amalia Naila Khairiyah Nurrahman, Tri Nurul Fitriyani Dewi Oktiani, Beta Widya Priyawan Rahmadi Rahma Dania A.J Rahmadella, Afifah Rahmadi, Priyawan Renie Kumala Dewi Robiyansyah, Hengki Rosihan Adhani, Rosihan Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Sitepu, Alexander Siti Khairiah Siti Raudah Siti Rohmah Tomy Wira Wahyuda Utami, Juliyatin Putri Vena Paramita Djunaidy Wardhana, Agung Satria Wydiamala, Erida Yuniar Elsa Dwinuria Yusrinie Wasiaturrahmah Zahra, Aisyah Nur

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