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EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN KECAPI (Sandoricum koetjape Merr) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis Yusrinie Wasiaturrahmah; Muhammad Rayhan; Deby Kania Tri Putri; Agung Satria Wardhana; Maharani Laillyza Apriasari
Jurnal Insan Farmasi Indonesia 2023: JIFI: Webinar & call for paper
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i3.1699

Penyebab terbesar kegagalan perawatan saluran akar sebesar 63% disebabkan oleh bakteri Enterococcus faecalis. Bakteri Enterococcus faecalis dapat dieliminasi dengan melakukan irigasi saluran akar. Kandungan zat aktif seperti saponin yang terdapat di dalam daun kecapi (Sandoricum koetjape Merr) terbukti memiliki efek antibakteri. Tujuan mengukur dan menganalisis efektivitas antibakteri ekstrak daun kecapi (Sandoricum koetjape Merr) terhadap pertumbuhan bakteri Enterococcus faecalis. Metode penelitian ini merupakan eksperimen murni (true experimental) dengan post test only with control group design. Penelitian ini terdiri dari 9 kelompok perlakuan, yaitu ekstrak daun kecapi (Sandoricum koetjape Merr) konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70%, sodium hipoklorit (NaOCl), dan aquadest dengan 3 kali pengulangan. Data KHM didapatkan dari pengukuran delta Optical Density (OD) dan data uji KBM didapatkan berdasarkan perhitungan jumlah koloni. Berdasarkan nilai rata-rata dan analisis data diketahui KHM terdapat pada konsentrasi ekstrak daun kecapi 10% dan KBM pada konsentrasi 20%. Ekstrak daun kecapi (Sandoricum koetjape Merr) konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, dan 70% memiliki efektivitas antibakteri terhadap pertumbuhan bakteri Enterococcus faecalis.

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study Dewi, Nurdiana; Rahmadella, Afifah; Hatta, Isnur; Apriasari, Maharani Laillyza; Putri, Deby Kania Tri
Padjadjaran Journal of Dentistry Vol 36, No 1 (2024): March 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no1.51018

ABSTRACTIntroduction: Caries is the most common oral disease found in society. The prevalence of caries in South Kalimantan is as high as 46.9% in 2018. S.mutans is the causative microorganism in the initial occurrence of caries. Strategy that can be used to prevent caries is by adding nano-hydroxyapatite to the tooth paste. Nano-hydroxyapatite can be obtained from Snakehead (Channa striata) fish bone. Snakehead is a kind of fish that is abundant in Banjarmasin. This study aimed to analyze antibacterial activity of the nano-hydroxyapatite paste from snakehead (Channa striata) fish bone against S.mutans bacteria. Methods: The study consisted of 5 treatment groups: negative control (basic formula), positive control (casein phosphopeptide-amorphous calcium phosphate or CPP-ACP) and three treatment groups (nano-hydroxyapatite paste concentration of 10, 20 and 30%. The paste was made in the formulation of F1, F2, and F3. The antibacterial activity test by measuring MIC and MBC were performed using dilution method. Results: MIC of nano-hydroxyapatite paste was at a concentration of 10% with an average value of the difference absorbance of -0.468. MIC values in the concentration of 10%, 15%, 20% positive and negative control groups had a significant difference. MBC of nano-hydroxyapatite paste was at concentration of 15%. Concentration of 10% and the negative control group showed a significant difference, while concentration of 15% and 20% groups did not show a significant difference. Conclusion: Nano-hydroxyapatite paste from Snakehead fish bone has antibacterial activity in inhibiting and eliminating mutated S.mutans bacteria. The most effective concentration of antibacterial nano-hydroxyapatite paste to prevent caries was 10%. At this concentration, nano-hydroxyapatite can inhibit the growth of S.mutans without killing the bacteria.KEYWORDS fish bone, nano-hydroxyapatite, snakehead, S.mutans

The Antibacterial Activity of Chitosan from Haruan (Channa striata) Fish Scales on the Growth of Streptococcus sanguinis Hutami, Wijayanti Diah Wasi; Tri Putri, Deby Kania; Carabelly, Amy Nindia; Kriswandini, Indah Listiana; Pratiwi, Ariyati Retno; Luthfi, Muhammad
Journal of Indonesian Dental Association Vol 3 No 2 (2020): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction: Recurrent Aphthous Stomatitis (RAS) is the most common oral lesion in Banjarmasin, with a prevalence of 45.42%. Streptococcus sanguinis (S. sanguinis) is thought to be one of the factors causing RAS. One natural ingredient that is often used by the people of South Kalimantan is the Haruan fish. The high Haruan fish consumption will eventually leave fish scales that have the potential to pollute the environment if not addressed immediately. Fish scales contain chitin, which when deacetylated produces chitosan, which has antibacterial properties. Objectives: The purpose of this study was to determine the effectiveness of the chitosan from the scales of the Haruan fish (Channa striata) at inhibiting S. sanguinis growth. Methods: This study used a randomized pretest-posttest with control group design using five treatments. The five treatments were subjected to liquid dilution using the UV-Vis Spectrophotometer method to obtain minimum inhibitory concentration (MIC) and a solid dilution test using the Total Plate Count method to obtain minimum bactericidal concentration (MBC). Results: The results showed that Haruan scale chitosan proved to be effective as an antibacterial against S. sanguinis, with a MIC of 1.25% and a minimum lethal concentration of 2.5%. One-Way Anova test results showed significance for the MIC test (p = 0,000) and MBC test (p = 0.000; p < 0.05). Conclusion: Chitosan from Haruan fish scales is both inhibitory and lethal to S. sanguinis.

Chitosan From Haruan (Channa Striata) Fish Scale Accelerate Wound Healing By Promoting Angiogenesis And Fibroblast Proliferation Putri, Deby Kania Tri; Fitriyana, Annisa; Raudah, Siti; Wardhana, Agung Satria; Wydiamala, Erida; Sarifah, Norlaila; Budipramana, Melisa
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.%p

Background: Damage to the integrity of some body tissues due to trauma commonly referred to as injury can occur intentionally or unintentionally. Angiogenesis and fibroblast proliferation are important stages in the proliferation stage in determining the success of the wound healing process. Chitosan from haruan fish scales has active functional groups in the form of anti-inflammatory and antioxidants that are effective in accelerating wound healing. Method: This study was a pure experimental study with a post-test only design with a control group design which was divided into a treatment group and a control group. The treatment group consisted of chitosan haruan fish scales at concentrations of 1%, 3%, and 5%. The control group consisted of povidone iodine as a positive control and no treatment as a negative control. All rats were injured on the back and then euthanized on days 3, 5, 7, and 14. Result: The chitosan from haruan fish scales affected the formation of new blood vessels and fibroblasts on day-3, increase the number of new blood vessels on day-5, decrease the number of new blood vessels accompanied by increase the number of fibroblasts on day-7, and also decrease the number of fibroblasts on day-14.Conclusion: 3%, and 5% chitosan from haruan fish scale proved to be effective against angiogenesis and fibroblast proliferation in wound healing.

Chitosan From Haruan (Channa Striata) Fish Scale Accelerate Wound Healing By Promoting Angiogenesis And Fibroblast Proliferation Putri, Deby Kania Tri; Fitriyana, Annisa; Raudah, Siti; Wardhana, Agung Satria; Wydiamala, Erida; Sarifah, Norlaila; Budipramana, Melisa
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.%p

Background: Damage to the integrity of some body tissues due to trauma commonly referred to as injury can occur intentionally or unintentionally. Angiogenesis and fibroblast proliferation are important stages in the proliferation stage in determining the success of the wound healing process. Chitosan from haruan fish scales has active functional groups in the form of anti-inflammatory and antioxidants that are effective in accelerating wound healing. Method: This study was a pure experimental study with a post-test only design with a control group design which was divided into a treatment group and a control group. The treatment group consisted of chitosan haruan fish scales at concentrations of 1%, 3%, and 5%. The control group consisted of povidone iodine as a positive control and no treatment as a negative control. All rats were injured on the back and then euthanized on days 3, 5, 7, and 14. Result: The chitosan from haruan fish scales affected the formation of new blood vessels and fibroblasts on day-3, increase the number of new blood vessels on day-5, decrease the number of new blood vessels accompanied by increase the number of fibroblasts on day-7, and also decrease the number of fibroblasts on day-14.Conclusion: 3%, and 5% chitosan from haruan fish scale proved to be effective against angiogenesis and fibroblast proliferation in wound healing.

BIODEGRADATION OF CHITOSAN MEMBRANE SCALES OF HARUAN FISH (Channa striata)-HYDROXYAPATITE IN ARTIFICIAL SALIVA SOLUTION Syifa Kamila; Deby Kania Tri Putri; Amy Nindia Carabelly; Melissa Budipramana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12192

Background: Membrane materials for surgical procedures using Guided Tissue Regeneration (GTR) are Polytetrafluoroethylene (PTFE) and collagen, but have the disadvantage of requiring further surgical procedures to remove membranes after use and rapid degradation properties. Haruan (Channa striata) fish scale chitosan is expected to be an alternative material for GTR membranes. Purpose: To determine the longer degradation time between chitosan membranes and chitosan+HA membranes in artificial saliva. Methods: This study used true experimental with post test only with control design using three treatments of 5% chitosan, 5% + 2 gr chitosan, and 5% + HA 4 gr of chitosan, positive control of GTR membrane with time intervals of 1, 7, 14, and 21 days in artificial saliva. Results: The results of the Kruskall Wallis test showed that there was a significant difference, the Mann Whitney Post Hoc test yielded 5% chitosan with chitosan+2 gr HA and chitosan+2gr HA with chitosan+4 gr HA had a p value <0.05 p=(0.000) in the 14th day, 5% chitosan with chitosan+4gr HA had a p value <0.05 p=(0.000) significant on days 7 and 14, and the positive control with all treatments had a p value <0.05 p=(0.000) . Conclusion: Chitosan and chitosan+HA membranes resulted in a degradation time of 21 days. Chitosan membrane + 2 g HA membrane was the best treatment because the remaining weight of the membrane was greater than the other treatments.Keywords : Channa striata, Chitosan, Degradation Test, Guided Tissue Regeneration (GTR), Hydroxyapatite, Remaining Membrane Weight

PENGARUH PERENDAMAN GIGI PADA KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN FOSFAT Muhammad Hafly Fariz Asyraq; Deby Kania Tri Putri; Tri Nurrahman; Agung Satria Wardhana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12201

Background: Chitosan solution from Haruan fish scales (Channa striata) can be used to maintain tooth enamel structure by reducing the rate of solubility of hydroxyapatite or tooth demineralization under acidic conditions. Chitosan haruan fish scale (Channa striata) has the potential to be an anti-demineralization agent. Objective: Analyze the effect of immersing teeth in chitosan solution of 2.5% and 5% Haruan fish scales (Channa striata) on levels of phosphate release. Method: This study used the true experimental method with a posttest-only control group design consisting of 3 treatment groups, namely the negative control, the 2.5% group and the 5% group. All groups were immersed in lactic acid solution pH 5.2. Measurement of phosphate release levels using a uv-vis spectrophotometer. Results: The results of the One Way Analysis of Variance (ANOVA) test obtained a value of p = 0.00 <0.05 which indicated that the hypothesis was accepted or that there was an effect of soaking the teeth in chitosan solution of Haruan fish scales (Channa striata) with a concentration of 2.5% and 5% on phosphate release . The Bonferroni Post Hoc Test showed that there were significant differences in all groups, namely the negative control group with the 2.5% and 5% groups, the 2.5% group and the 5% group. Conclusion: Chitosan from Haruan fish scales (Channa striata) concentrations of 2.5% and 5% are able to inhibit phosphate release on teeth and have the potential as alternative biomaterials in inhibiting tooth demineralization.Keywords: Caries, Chitosan, Demineralization, Haruan, Phosphate

PENGARUH PERENDAMAN GIGI DESIDUI PADA LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN ION KALSIUM Noval Ihza Maulana; Didit Aspriyanto; Deby Kania Tri Putri; Yusrinie Wasiaturrahmah; Sherli Diana
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13109

ABSTRACTBackground: Haruan fish (Channa striata) scale chitosan solution can maintain tooth structure by reducing the solubility rate of hydroxyapatite which makes up tooth enamel. Haruan fish (Channa striata) scale chitosan solution prevents tooth demineralization in an acidic environment. Objective: To analyze the effect of soaking deciduous teeth in chitosan solution from haruan fish scales (Channa striata) with concentrations of 1.25%, 2.5% and 5% on the release of calcium ions in an acidic environment. Method: Pure experimental research with a post-test only design with control group, consisting of 4 treatment groups. The negative control was immersion of deciduous teeth in lactic acid solution pH 5.2, and treatment groups with concentrations of 1.25%, 2.5% and 5%. All groups soaked deciduous teeth in lactic acid solution with a pH of 5.2 for each group. Measurement of calcium release levels in deciduous teeth using UV-Vis spectrophotometry instruments. Results: The results of this study are hypothesized to be acceptable or that there is an effect of soaking deciduous teeth in haruan fish scale chitosan (Channa striata) concentrations of 1.25%, 2.5% and 5% in inhibiting the release of calcium ions. This study showed that there were no significant differences between the 1.25%, 2.5% and 5% treatment groups, but there were significant differences between the control group and all treatment groups. Conclusion: Haruan fish (Channa striata) scale chitosan with concentrations of 1.25%, 2.5%, and 5% is able to inhibit the release of calcium in deciduous teeth and has potential as a demineralization inhibitor biomaterial.Keywords : Caries, Calcium, Chitosan, Demineralization, Haruan. ABSTRAK Latar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) dapat menjaga struktur gigi melalui proses penurunan laju kelarutan hidroksiapatit penyusun enamel gigi. Larutan kitosan sisik ikan haruan (Channa striata) mencegah demineralisasi gigi dalam lingkungan asam. Tujuan: Menganalisis pengaruh perendaman gigi desidui pada larutan kitosan dari sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% terhadap pelepasan ion kalsium dalam lingkungan asam. Metode: Penelitian eksperimental murni dengan desain post-test only with control group, terdiri dari 4 kelompok perlakuan. Kontrol negatif yaitu perendaman gigi desidui pada larutan asam laktat pH 5,2, dan kelompok perlakuan konsentrasi 1,25%, 2,5%, serta 5%. Semua kelompok perendaman gigi desidui dalam larutan asam laktat dengan pH 5,2 dilakukan pada setiap kelompok. Pengukuran kadar pelepasan kalsium gigi desidui menggunakan instrumen spektrofotometri UV-Vis. Hasil: Hasil penelitian ini hipotesis dapat diterima atau adanya pengaruh perendaman gigi desidui pada kitosan sisik ikan haruan (Channa striata) konsentrasi 1,25%, 2,5%, dan 5% dalam menghambat pelepasan ion kalsium. Pada penelitian ini menunjukkan tidak adanya perbedaan signifikan antar kelompok perlakuan 1,25%, 2,5%, dan 5%, namun terdapat perbedaan bermakna antara kelompok kontrol dengan semua kelompok perlakuan. Kesimpulan: Kitosan sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% mampu menghambat pelepasan kalsium pada gigi desidui dan memiliki potensi sebagai biomaterial agen penghambat demineralisasi. Kata kunci : Demineralisasi, Haruan, Kalsium, Karies, Kitosan

The Antibacterial Activity of Chitosan from Haruan (Channa striata) Fish Scales on the Growth of Streptococcus sanguinis Wijayanti Diah Wasi Hutami; Deby Kania Tri Putri; Amy Nindia Carabelly; Indah Listiana Kriswandini; Ariyati Retno Pratiwi; Muhammad Luthfi
Journal of Indonesian Dental Association Vol. 3 No. 2 (2020): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v3i2.450

Introduction: Recurrent Aphthous Stomatitis (RAS) is the most common oral lesion in Banjarmasin, with a prevalence of 45.42%. Streptococcus sanguinis (S. sanguinis) is thought to be one of the factors causing RAS. One natural ingredient that is often used by the people of South Kalimantan is the Haruan fish. The high Haruan fish consumption will eventually leave fish scales that have the potential to pollute the environment if not addressed immediately. Fish scales contain chitin, which when deacetylated produces chitosan, which has antibacterial properties. Objectives: The purpose of this study was to determine the effectiveness of the chitosan from the scales of the Haruan fish (Channa striata) at inhibiting S. sanguinis growth. Methods: This study used a randomized pretest-posttest with control group design using five treatments. The five treatments were subjected to liquid dilution using the UV-Vis Spectrophotometer method to obtain minimum inhibitory concentration (MIC) and a solid dilution test using the Total Plate Count method to obtain minimum bactericidal concentration (MBC). Results: The results showed that Haruan scale chitosan proved to be effective as an antibacterial against S. sanguinis, with a MIC of 1.25% and a minimum lethal concentration of 2.5%. One-Way Anova test results showed significance for the MIC test (p = 0,000) and MBC test (p = 0.000; p < 0.05). Conclusion: Chitosan from Haruan fish scales is both inhibitory and lethal to S. sanguinis.

TOXICITY TESTING OF CHITOSAN OF PAPUYU FISH SCALE (Anabas testudineus) TOWARD BHK-21 FIBROBLAST CELLS Siti Rohmah; Muhammad Yanuar Ichrom Nahzi; Deby Kania Tri Putri
Dentino: Jurnal Kedokteran Gigi Vol 9, No 1 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i1.18857

Background: Pulp capping is a treatment that aims to maintain the viability of the pulp tissue. Calcium hydroxide (Ca(OH)2 is a material that is often used for pulp capping procedures. However, this material does not provide a good adaptation to dentin. Therefore, an alternative material that is more biocompatible is needed, one of which is by using papuyu fish scale chitosan. papuyu fish as an alternative material must be proven to have no toxic effects. Purpose: to analyze whether the chitosan of papuyu fish scales (Anabas testudineus) is toxic to BHK-21 fibroblast cells using the MTT assay method. Methods: This study was a pure experimental study with a post-test-only design with a control group design consisting of 12 groups. The group given the chitosan treatment of papuyu fish scales consisted of 10 groups with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% with 2 control groups namely control cells. and media control. Results: The results showed that the chitosan of papuyu fish scales did not have a toxic effect on BHK-21 fibroblast cells because the percentage value of cell viability of the entire treatment group was > 60% and the IC50 value was > 0.1%, which was 5.405 %. Conclusion: There was no toxic effect after administration of papuyu fish scales chitosan (Anabas testudineus) with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% on cells BHK-21 fibroblasts. Keywords: BHK-21 fibroblast cells, Papuyu fish scale chitosan, Toxicity test

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