Tri Putri, Deby Kania
Department Of Biomedic, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin

Published : 43 Documents Claim Missing Document
Claim Missing Document
Check
Articles

PENGARUH PERENDAMAN GIGI DESIDUI PADA LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN ION KALSIUM Noval Ihza Maulana; Didit Aspriyanto; Deby Kania Tri Putri; Yusrinie Wasiaturrahmah; Sherli Diana
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13109

Abstract

ABSTRACTBackground: Haruan fish (Channa striata) scale chitosan solution can maintain tooth structure by reducing the solubility rate of hydroxyapatite which makes up tooth enamel. Haruan fish (Channa striata) scale chitosan solution prevents tooth demineralization in an acidic environment. Objective: To analyze the effect of soaking deciduous teeth in chitosan solution from haruan fish scales (Channa striata) with concentrations of 1.25%, 2.5% and 5% on the release of calcium ions in an acidic environment. Method: Pure experimental research with a post-test only design with control group, consisting of 4 treatment groups. The negative control was immersion of deciduous teeth in lactic acid solution pH 5.2, and treatment groups with concentrations of 1.25%, 2.5% and 5%. All groups soaked deciduous teeth in lactic acid solution with a pH of 5.2 for each group. Measurement of calcium release levels in deciduous teeth using UV-Vis spectrophotometry instruments. Results: The results of this study are hypothesized to be acceptable or that there is an effect of soaking deciduous teeth in haruan fish scale chitosan (Channa striata) concentrations of 1.25%, 2.5% and 5% in inhibiting the release of calcium ions. This study showed that there were no significant differences between the 1.25%, 2.5% and 5% treatment groups, but there were significant differences between the control group and all treatment groups. Conclusion: Haruan fish (Channa striata) scale chitosan with concentrations of 1.25%, 2.5%, and 5% is able to inhibit the release of calcium in deciduous teeth and has potential as a demineralization inhibitor biomaterial.Keywords :  Caries, Calcium, Chitosan, Demineralization, Haruan.  ABSTRAK Latar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) dapat menjaga struktur gigi melalui proses penurunan laju kelarutan hidroksiapatit penyusun enamel gigi. Larutan kitosan sisik ikan haruan (Channa striata) mencegah demineralisasi gigi dalam lingkungan asam. Tujuan: Menganalisis pengaruh perendaman gigi desidui pada larutan kitosan dari sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% terhadap pelepasan ion kalsium dalam lingkungan asam. Metode: Penelitian eksperimental murni dengan desain post-test only with control group, terdiri dari 4 kelompok perlakuan. Kontrol negatif yaitu perendaman gigi desidui pada larutan asam laktat pH 5,2, dan kelompok perlakuan konsentrasi 1,25%, 2,5%, serta 5%. Semua kelompok perendaman gigi desidui dalam larutan asam laktat dengan pH 5,2 dilakukan pada setiap kelompok. Pengukuran kadar pelepasan kalsium gigi desidui menggunakan instrumen spektrofotometri UV-Vis. Hasil: Hasil penelitian ini hipotesis dapat diterima atau adanya pengaruh perendaman gigi desidui pada kitosan sisik ikan haruan (Channa striata) konsentrasi 1,25%, 2,5%, dan 5% dalam menghambat pelepasan ion kalsium. Pada penelitian ini menunjukkan tidak adanya perbedaan signifikan antar kelompok perlakuan 1,25%, 2,5%, dan 5%, namun terdapat perbedaan bermakna antara kelompok kontrol dengan semua kelompok perlakuan. Kesimpulan: Kitosan sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% mampu menghambat pelepasan kalsium pada gigi desidui dan memiliki potensi sebagai biomaterial agen penghambat demineralisasi. Kata kunci : Demineralisasi, Haruan, Kalsium, Karies, Kitosan
ANTIBACTERIAL ACTIVITY OF PAPUYU FISH SCALES CHITOSAN (Anabas testudineus) AGAINST Enterococcus faecalis Haliza, Ninda Andrea; Ichrom Nahzi, Muhammad Yanuar; Sitepu, Alexander; Dewi, Nurdiana; Tri Putri, Deby Kania
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22196

Abstract

ABSTRACTBackground: Papuyu fish is widely consumed by Kalimantan people. Papuyu fish scales chitosan has antibacterial potential because it contains amino polysaccharides and enzyme lysozyme which inhibits the growth of root canal bacteria, specifically Enterococcus faecalis. Purpose: The purpose of this experimental is to analyze the antibacterial activity of papuyu fish (Anabas testudineus) scales chitosan against Enterococcus faecalis. Methods: This is a true experimental study with post-test only with a control group design using 15 samples of papuyu fish (Anabas testudineus) scales chitosan grouped into 5 treatment groups and 3 samples repetitions. The treatment groups consisted of papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100%, and distilled water (negative control). Absorbance calculation using a UV-Vis Spectrophotometer 722 for minimum inhibitory concentration (MIC) and Enterococcus faecalis colonies are visualized with a colony counter for minimum bactericidal concentration (MBC). Results: The MIC value of papuyu fish (Anabas testudineus) scales chitosan were obtained at 25% concentration and the MBC value were obtained at 100% concentration. The results of One-Way ANOVA test for MIC and Kruskal Wallis for MBC of papuyu fish scales chitosan concentrations of 25%, 50%, 75%, and 100% obtained significant results of p<0,05 and showed a significant difference between treatments. Conclusion: These are differences in the effectiveness of MIC and MBC papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100% and distilled water. Papuyu fish scales chitosan 100% concentration is the most effective concentration as an antibacterial against the growth of Enterococcus faecalis.
NANO-HYDROXYAPATITE AND HYDROXYCHAVICOL'S COMBINED EFFECT IN Streptococcus mutans GROWTH Dewi, Nurdiana; Oktiani, Beta Widya; Tri Putri, Deby Kania; Amelia, Aura; Zahra, Aisyah Nur
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22207

Abstract

Background: Early childhood caries is an aggressive form of dental caries that affects children under six years old. The increasing prevalence of ECC caused by Streptococcus mutans highlights the need for effective antimicrobial agents. Objective: To evaluate the antibacterial activity of a combination of nanohydroxyapatite (nHAp) and hydroxychavicol (HC) against S. mutans. Methods: 100 mg/mL nHAp was combined with HC at 0.125, 0.25, 0.5, and 1 mg/mL concentrations. DMSO 5% was used as the negative control. S. mutans ATCC 25175 was cultured in Brain Heart Infusion (BHI) media. Minimum Inhibitory Concentration (MIC) was determined using a UV-Vis spectrophotometer to measure absorbance differences. Minimum Bactericidal Concentration (MBC) was established by plating onto BHI agar and counting the colony count.  Results: The results showed that MIC occurred at a combination of nHAp 100 mg/mL + HC 0.125 mg/mL, while MBC was achieved at nHAp 100 mg/mL + 1 mg/mL HC, where no bacterial colonies were observed. Statistical analysis using ANOVA and Bonferroni post-hoc tests revealed significant differences in both absorbance reduction and colony counts between groups (p < 0.05), confirming the dose-dependent antibacterial efficacy of the nHAp-HC combination. Conclusion: These findings indicate that the synergistic action of nHAp and HC offers a promising strategy for controlling ECC, particularly by targeting the growth of S. mutans. Further, in vivo studies are recommended to explore clinical applicability and long-term safety.
Co-Authors Agung Satria Wardhana Aizar Agi Syahrial Aizar Agi Syahrial, Aizar Agi Amalia Putri, Amalia Amelia, Aura Amy Nindia Carabelly Ariyati Retno Pratiwi, Ariyati Retno Aspriyanto, Didit Aulia Azizah Aulia Rahimah Azilita Ananda Azwar Fida Maulana Baehaqi Bambang Setiawan Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Candra Candra Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Diana Wibowo Duhan Kanzu Balad Eny Febriyanti Erika Norfitriah Fatimah Maulideya Fatma Kirana Ferdy Juliannor Fajar Firdaus, I Wayan Arya Krishnawan Fitriyana, Annisa Haliza, Ninda Andrea Hamdani, Riky Hatta, Isnur Hutami, Wijayanti Diah Wasi Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indah Listiana Kriswandini Jamilatun Nisa Juli Harnida Purwaningayu K. Khairiah Maharani Laillyza Apriasari Melati Ainun Nisa Melisa Budipramana, Melisa Melissa Budipramana Moch. Andhika Firdausi Rachfa Muhammad Alfi Ghifari Muhammad Hafly Fariz Asyraq Muhammad Irfan Fanshuri Muhammad Luthfi Muhammad Luthfi MUHAMMAD RAYHAN Muhammad Zainal Fikri Nadalia Malika Bilqis Nadiya Amalia Naila Khairiyah Noval Ihza Maulana Nurrahman, Tri Nurul Fitriyani Dewi Oktiani, Beta Widya Priyawan Rahmadi Rahma Dania A.J Rahmadella, Afifah Rahmadi, Priyawan Renie Kumala Dewi Robiyansyah, Hengki Rosihan Adhani, Rosihan Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Sitepu, Alexander Siti Khairiah Siti Raudah Siti Rohmah Syifa Kamila Tomy Wira Wahyuda Utami, Juliyatin Putri Vena Paramita Djunaidy Wardhana, Agung Satria Wydiamala, Erida Yuniar Elsa Dwinuria Yusrinie Wasiaturrahmah Zahra, Aisyah Nur