Tri Putri, Deby Kania
Department Of Biomedic, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin

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BIODEGRADATION OF CHITOSAN MEMBRANE SCALES OF HARUAN FISH (Channa striata)-HYDROXYAPATITE IN ARTIFICIAL SALIVA SOLUTION Syifa Kamila; Deby Kania Tri Putri; Amy Nindia Carabelly; Melissa Budipramana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12192

Abstract

Background: Membrane materials for surgical procedures using Guided Tissue Regeneration (GTR) are Polytetrafluoroethylene (PTFE) and collagen, but have the disadvantage of requiring further surgical procedures to remove membranes after use and rapid degradation properties. Haruan (Channa striata) fish scale chitosan is expected to be an alternative material for GTR membranes. Purpose: To determine the longer degradation time between chitosan membranes and chitosan+HA membranes in artificial saliva. Methods: This study used true experimental with post test only with control design using three treatments of 5% chitosan, 5% + 2 gr chitosan, and 5% + HA 4 gr of chitosan, positive control of GTR membrane with time intervals of 1, 7, 14, and 21 days in artificial saliva. Results: The results of the Kruskall Wallis test showed that there was a significant difference, the Mann Whitney Post Hoc test yielded 5% chitosan with chitosan+2 gr HA and chitosan+2gr HA with chitosan+4 gr HA had a p value <0.05 p=(0.000) in the 14th day, 5% chitosan with chitosan+4gr HA had a p value <0.05 p=(0.000) significant on days 7 and 14, and the positive control with all treatments had a p value <0.05 p=(0.000) . Conclusion: Chitosan and chitosan+HA membranes resulted in a degradation time of 21 days. Chitosan membrane + 2 g HA membrane was the best treatment because the remaining weight of the membrane was greater than the other treatments.Keywords : Channa striata, Chitosan, Degradation Test, Guided Tissue Regeneration (GTR), Hydroxyapatite, Remaining Membrane Weight
PENGARUH PERENDAMAN GIGI PADA KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN FOSFAT Muhammad Hafly Fariz Asyraq; Deby Kania Tri Putri; Tri Nurrahman; Agung Satria Wardhana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12201

Abstract

Background: Chitosan solution from Haruan fish scales (Channa striata) can be used to maintain tooth enamel structure by reducing the rate of solubility of hydroxyapatite or tooth demineralization under acidic conditions. Chitosan haruan fish scale (Channa striata) has the potential to be an anti-demineralization agent. Objective: Analyze the effect of immersing teeth in chitosan solution of 2.5% and 5% Haruan fish scales (Channa striata) on levels of phosphate release. Method: This study used the true experimental method with a posttest-only control group design consisting of 3 treatment groups, namely the negative control, the 2.5% group and the 5% group. All groups were immersed in lactic acid solution pH 5.2. Measurement of phosphate release levels using a uv-vis spectrophotometer. Results: The results of the One Way Analysis of Variance (ANOVA) test obtained a value of p = 0.00 <0.05 which indicated that the hypothesis was accepted or that there was an effect of soaking the teeth in chitosan solution of Haruan fish scales (Channa striata) with a concentration of 2.5% and 5% on phosphate release . The Bonferroni Post Hoc Test showed that there were significant differences in all groups, namely the negative control group with the 2.5% and 5% groups, the 2.5% group and the 5% group. Conclusion: Chitosan from Haruan fish scales (Channa striata) concentrations of 2.5% and 5% are able to inhibit phosphate release on teeth and have the potential as alternative biomaterials in inhibiting tooth demineralization.Keywords: Caries, Chitosan, Demineralization, Haruan, Phosphate
PENGARUH PERENDAMAN GIGI DESIDUI PADA LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN ION KALSIUM Noval Ihza Maulana; Didit Aspriyanto; Deby Kania Tri Putri; Yusrinie Wasiaturrahmah; Sherli Diana
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13109

Abstract

ABSTRACTBackground: Haruan fish (Channa striata) scale chitosan solution can maintain tooth structure by reducing the solubility rate of hydroxyapatite which makes up tooth enamel. Haruan fish (Channa striata) scale chitosan solution prevents tooth demineralization in an acidic environment. Objective: To analyze the effect of soaking deciduous teeth in chitosan solution from haruan fish scales (Channa striata) with concentrations of 1.25%, 2.5% and 5% on the release of calcium ions in an acidic environment. Method: Pure experimental research with a post-test only design with control group, consisting of 4 treatment groups. The negative control was immersion of deciduous teeth in lactic acid solution pH 5.2, and treatment groups with concentrations of 1.25%, 2.5% and 5%. All groups soaked deciduous teeth in lactic acid solution with a pH of 5.2 for each group. Measurement of calcium release levels in deciduous teeth using UV-Vis spectrophotometry instruments. Results: The results of this study are hypothesized to be acceptable or that there is an effect of soaking deciduous teeth in haruan fish scale chitosan (Channa striata) concentrations of 1.25%, 2.5% and 5% in inhibiting the release of calcium ions. This study showed that there were no significant differences between the 1.25%, 2.5% and 5% treatment groups, but there were significant differences between the control group and all treatment groups. Conclusion: Haruan fish (Channa striata) scale chitosan with concentrations of 1.25%, 2.5%, and 5% is able to inhibit the release of calcium in deciduous teeth and has potential as a demineralization inhibitor biomaterial.Keywords :  Caries, Calcium, Chitosan, Demineralization, Haruan.  ABSTRAK Latar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) dapat menjaga struktur gigi melalui proses penurunan laju kelarutan hidroksiapatit penyusun enamel gigi. Larutan kitosan sisik ikan haruan (Channa striata) mencegah demineralisasi gigi dalam lingkungan asam. Tujuan: Menganalisis pengaruh perendaman gigi desidui pada larutan kitosan dari sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% terhadap pelepasan ion kalsium dalam lingkungan asam. Metode: Penelitian eksperimental murni dengan desain post-test only with control group, terdiri dari 4 kelompok perlakuan. Kontrol negatif yaitu perendaman gigi desidui pada larutan asam laktat pH 5,2, dan kelompok perlakuan konsentrasi 1,25%, 2,5%, serta 5%. Semua kelompok perendaman gigi desidui dalam larutan asam laktat dengan pH 5,2 dilakukan pada setiap kelompok. Pengukuran kadar pelepasan kalsium gigi desidui menggunakan instrumen spektrofotometri UV-Vis. Hasil: Hasil penelitian ini hipotesis dapat diterima atau adanya pengaruh perendaman gigi desidui pada kitosan sisik ikan haruan (Channa striata) konsentrasi 1,25%, 2,5%, dan 5% dalam menghambat pelepasan ion kalsium. Pada penelitian ini menunjukkan tidak adanya perbedaan signifikan antar kelompok perlakuan 1,25%, 2,5%, dan 5%, namun terdapat perbedaan bermakna antara kelompok kontrol dengan semua kelompok perlakuan. Kesimpulan: Kitosan sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% mampu menghambat pelepasan kalsium pada gigi desidui dan memiliki potensi sebagai biomaterial agen penghambat demineralisasi. Kata kunci : Demineralisasi, Haruan, Kalsium, Karies, Kitosan
Co-Authors Agung Satria Wardhana Aisyah Nur Zahra Aizar Agi Syahrial Aizar Agi Syahrial, Aizar Agi Alexander Sitepu Amalia Putri, Amalia Amy Nindia Carabelly Annisa Rahma Ariyati Retno Pratiwi, Ariyati Retno Aspriyanto, Didit Aulia Azizah Aulia Rahimah Aura Amelia Azilita Ananda Azwar Fida Maulana Bambang Setiawan Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Candra Candra Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Diana Wibowo Duhan Kanzu Balad Eny Febriyanti Erika Norfitriah Fatimah Maulideya Fatma Kirana Ferdy Juliannor Fajar Firdaus, I Wayan Arya Krishnawan Fitriyana, Annisa Hamdani, Riky Hatta, Isnur Hutami, Wijayanti Diah Wasi Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indah Listiana Kriswandini Jamilatun Nisa Juli Harnida Purwaningayu K. Khairiah Maharani Laillyza Apriasari Melati Ainun Nisa Melisa Budipramana, Melisa Melissa Budipramana Moch. Andhika Firdausi Rachfa Muhammad Alfi Ghifari Muhammad Hafly Fariz Asyraq Muhammad Irfan Fanshuri Muhammad Luthfi Muhammad Luthfi MUHAMMAD RAYHAN Muhammad Zainal Fikri Nadalia Malika Bilqis Nadiya Amalia Naila Khairiyah Ninda Andrea Haliza Noval Ihza Maulana Nurrahman, Tri Nurul Fitriyani Dewi Priyawan Rahmadi Rahma Dania A.J Rahmadella, Afifah Rahmadi, Priyawan Renie Kumala Dewi Robiyansyah, Hengki Rosihan Adhani, Rosihan Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Siti Khairiah Siti Raudah Siti Rohmah Syifa Kamila Tomy Wira Wahyuda Utami, Juliyatin Putri Vena Paramita Djunaidy Wardhana, Agung Satria Wydiamala, Erida Yuniar Elsa Dwinuria Yusrinie Wasiaturrahmah