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Journal : Pharmasipha

Aktivitas Antibakteri Fraksi Aquades, Diklorometana dan N-Heksana Daun Majapahit (Crescentia cujete L.) terhadap Bakteri Escherichia coli ATCC 25922 Secara In-Vitro Choirul Huda; Rahma Diyan Martha; Niken Desi Wulandhari
Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy Vol 6, No 2 (2022): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v6i2.8495

Abstract

Escherichia coli is a gram - negative bacteria which is able to cause infectious diseases in humans, including urina ry tract infections, stomach cramps, and diarrhea. The purpose of this study was to determine the antibacterial activity of the Majapahit leaf fraction and the optimum concentration to inhibit Escherichia coli bacteria. Majapahit leaves were extracted by t he maceration method using 70% ethanol f ollowed by fractionation using aqua dest solvent, dichloromethane, and n - Hexane. Phytochemical screening of Majapahit leaf extract for the content of flavonoids, alkaloids, and saponins. Antibacterial activity test us ing paper disc diffusion method with positive control of chloramphenicol and negative control of 10% DMSO. The results of phytochemical screening of extracts were positive to flavonoid compounds, alkaloids, and saponins. The results of the antibacterial ac tivity test of the Majapahit leaf fraction had an antibacterial activity which was indicated by the presence of a clear zone around the disc. The aqua dest fraction is the most active fraction that can inhibit Escherichia coli bacteria. Based on the data a nalysis that has been carried out, it can be concluded that the use of different types of solvents did not produce significant differences in inhibiting Escherichia coli bacteria.
Aktivitas Antibakteri Fraksi Aquades, Diklorometana dan N-Heksana Daun Majapahit (Crescentia cujete L.) terhadap Bakteri Escherichia coli ATCC 25922 Secara In-Vitro Huda, Choirul; Martha, Rahma Diyan; Wulandhari, Niken Desi
Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy Vol. 6 No. 2 (2022): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v6i2.8495

Abstract

Escherichia coli is a gram - negative bacteria which is able to cause infectious diseases in humans, including urina ry tract infections, stomach cramps, and diarrhea. The purpose of this study was to determine the antibacterial activity of the Majapahit leaf fraction and the optimum concentration to inhibit Escherichia coli bacteria. Majapahit leaves were extracted by t he maceration method using 70% ethanol f ollowed by fractionation using aqua dest solvent, dichloromethane, and n - Hexane. Phytochemical screening of Majapahit leaf extract for the content of flavonoids, alkaloids, and saponins. Antibacterial activity test us ing paper disc diffusion method with positive control of chloramphenicol and negative control of 10% DMSO. The results of phytochemical screening of extracts were positive to flavonoid compounds, alkaloids, and saponins. The results of the antibacterial ac tivity test of the Majapahit leaf fraction had an antibacterial activity which was indicated by the presence of a clear zone around the disc. The aqua dest fraction is the most active fraction that can inhibit Escherichia coli bacteria. Based on the data a nalysis that has been carried out, it can be concluded that the use of different types of solvents did not produce significant differences in inhibiting Escherichia coli bacteria.
Co-Authors 'Aini, Febriana Devi Nur - Fatimah Adisyaningrum, Ega Nurgia Aena Laili Afidatul Muadifah Afidatul Muadifah Ahmad Yudianto Algafari Bakti Manggara Amalia Putri Eka Anas Fadli Wijaya Anisa Azizah Annisah Mahanani Aprilia, Chatrina Apriliani, Herlina Arif Santoso Arif Santoso Asmarani Kusumawati Atiqoh Zummah Ayu Insa Ayu Insa Fajri Azriel Restu Azriel Restu Chairil Anwar Choirul Huda Choirul Huda Danar Danar Danar Danar Danar, Danar Dara Pranidya Tilarso Dita Arindea Tunggal Putri Dyah Setyaningrum, Dyah Ega Nurgia Adisyaningrum Ega Nurgia Adisyaningrum Elen Vikelavianis Elen Vikelavianis Ema Krismawar Sari Ema Krismawar Sari Fadila, Natasya Nurul Fajri, Ayu Insa Fatimah fatimah Fatimah Fatimah Fatimah Fatimah Fatimah Fuzianingsih, Eka Novia Hesty Parbuntari ika Rachutami Ika Rachutami Ita Rhosida Ita Rhosida Jalu Prakoso Jalu Prakoso Kartika Arum Wardani Kharisma, Mei Nabila Revina Kholifah, Siti Nurriyatul Kristianto, Sonny Kristina Handayani Kumalasari, Nurul Chamidah Lestari, Nasa Bela Dwi Maida, Margarita Claudya Mashudi Mashudi Maya Diva Maya Diva Siswidiani Muadifah, Afidatul Muadifah, Afidhatul Mutia Hariani Murjanah Mutia Hariani Nurjanah Nasa Bella Ngibad, Khoirul Niken Desi Wulandhari Nurin, Wachidatun Nurjanah, Mutia Hariani Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Parbuntari, Hesty Parbuntary, Hesty Putri, Amalia Eka Rahmadani, Anindita Mutiara Rahmawati, Ayu Sintya Citra Ramadhani, Melati Apriliana Retnosari, Rini Rian Anggia Wijaya Rury Eryna Putri Safitri, Yunita Diyah Sefrina Sefrina Siti Awwalul Amanatur Rohmah Siti Nurriyatul Kholifah Sri Wahyuningsih SULASTRI Sulastri Sulastri Tilarso, Dara Pranidya Tutik Dwi Wahyuningsih Ulayya, Nida Asma vifta, rissa laila Widayanti, Sindi Dwi Wimbuh Tri Widodo Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Wulandhari, Niken Desi Yuliandari, Fadhira Yulindasari, Armita Regina Putri