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All Journal Jurnal Penelitian Saintek Sainteks Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Majalah Obat Tradisional CHEMPUBLISH JOURNAL Jurnal Wiyata : Penelitian Sains dan Kesehatan Pharmasipha CHEESA: Chemical Engineering Research Articles JOPS (Journal Of Pharmacy and Science) JURNAL PHOTON Walisongo Journal of Chemistry Jurnal Kreativitas PKM Eksakta : Berkala Ilmiah Bidang MIPA Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Cendekia : Jurnal Pengabdian Masyarakat Stannum : Jurnal Sains dan Terapan Kimia Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang JURNAL BIOSHELL PROFESSIONAL HEALTH JOURNAL Jurnal Pengabdian Masyarakat (ABDIRA) Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Gemakes: Jurnal Pengabdian Kepada Masyarakat Gunung Djati Conference Series EJOIN : Jurnal Pengabdian Masyarakat Indonesian Journal of Pharmacy and Natural Product Prosiding Seminar Nasional Unimus
Rahma Diyan Martha
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Journal : PROFESSIONAL HEALTH JOURNAL

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Identification of Antibacterial Compounds in Methanol Extract of Clove Stems (Syzygium aromaticum) using Liquid Chromatography Mass Spectrometry Yunita Diyah Safitri; Rahma Diyan Martha; Mutia Hariani Nurjanah
PROFESSIONAL HEALTH JOURNAL Vol. 6 No. 2 (2025): In Progress issue
Publisher : Pusat Penelitian dan Pengabdian Masyarakat (PPPM) STIKES Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54832/phj.v6i2.897

Abstract

Introduction: Clove is well-known as an herbal plant that is also included in the spice group which is known to have an antibacterial activity spread across the stems, leaves, and flowers. Objectives: The purpose of this study was to identify the antibacterial compounds that are found in the methanol extract of clove stems and the antibacterial activity of clove stem extract against Bacillus cereus. Methods: We used Liquid Chromatography Mass Spectrometry (LC-MS) method to identify the antibacterial compounds, and used the paper disc method (Kirby Bauer) for antibacterial test with clove stem extract concentrations of 30%, 50%, and 70%, positive control of Amoxicillin at a dose of 0.5 mg/ml, also a negative control of sterile aquadest. Results: The results showed four antibacterial compounds in the extract, namely (+)-Licarin A, 4-O-Caffeoylquinic acid_1, Eckol, and Leucodelphinidin. Methanol extract of clove stems at the concentrations of 30%, 50%, and 70% inhibited the growth of Bacillus cereus significantly (p <0.05). The concentration of 30% was categorized as quite sensitive, meanwhile, both 50% and 70% were categorized as sensitive. Conclusions: In conclusion, four compounds with antibacterial properties were found in the methanol extract of clove stems based on LCMS analysis, and supported by the results of antibacterial tests which showed the formation of an inhibition zone in the clove stem extract against the growth of Bacillus cereus.
Identification of Antibacterial Compounds in Methanol Extract of Clove Stems (Syzygium aromaticum) using Liquid Chromatography Mass Spectrometry Yunita Diyah Safitri; Rahma Diyan Martha; Mutia Hariani Nurjanah
PROFESSIONAL HEALTH JOURNAL Vol. 6 No. 2 (2025): June
Publisher : Pusat Penelitian dan Pengabdian Masyarakat (PPPM) STIKES Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54832/phj.v6i2.897

Abstract

Introduction: Clove is well-known as an herbal plant that is also included in the spice group which is known to have an antibacterial activity spread across the stems, leaves, and flowers. Objectives: The purpose of this study was to identify the antibacterial compounds that are found in the methanol extract of clove stems and the antibacterial activity of clove stem extract against Bacillus cereus. Methods: We used Liquid Chromatography Mass Spectrometry (LC-MS) method to identify the antibacterial compounds, and used the paper disc method (Kirby Bauer) for antibacterial test with clove stem extract concentrations of 30%, 50%, and 70%, positive control of Amoxicillin at a dose of 0.5 mg/ml, also a negative control of sterile aquadest. Results: The results showed four antibacterial compounds in the extract, namely (+)-Licarin A, 4-O-Caffeoylquinic acid_1, Eckol, and Leucodelphinidin. Methanol extract of clove stems at the concentrations of 30%, 50%, and 70% inhibited the growth of Bacillus cereus significantly (p <0.05). The concentration of 30% was categorized as quite sensitive, meanwhile, both 50% and 70% were categorized as sensitive. Conclusions: In conclusion, four compounds with antibacterial properties were found in the methanol extract of clove stems based on LCMS analysis, and supported by the results of antibacterial tests which showed the formation of an inhibition zone in the clove stem extract against the growth of Bacillus cereus.
Co-Authors 'Aini, Febriana Devi Nur - Fatimah Adisyaningrum, Ega Nurgia Aena Laili Afidatul Muadifah Afidatul Muadifah Ahmad Yudianto Algafari Bakti Manggara Amalia Putri Eka Anas Fadli Wijaya Anisa Azizah Annisah Mahanani Aprilia, Chatrina Apriliani, Herlina Arif Santoso Arif Santoso Asmarani Kusumawati Atiqoh Zummah Ayu Insa Ayu Insa Fajri Azriel Restu Azriel Restu Chairil Anwar Choirul Huda Choirul Huda Danar Danar Danar Danar Danar, Danar Dara Pranidya Tilarso Dita Arindea Tunggal Putri Dyah Setyaningrum, Dyah Ega Nurgia Adisyaningrum Ega Nurgia Adisyaningrum Elen Vikelavianis Elen Vikelavianis Ema Krismawar Sari Ema Krismawar Sari Fadila, Natasya Nurul Fajri, Ayu Insa Fatimah Fatimah Fatimah fatimah Fatimah Fatimah Fatimah Fuzianingsih, Eka Novia Hesty Parbuntari ika Rachutami Ika Rachutami Ita Rhosida Ita Rhosida Jalu Prakoso Jalu Prakoso Kartika Arum Wardani Kharisma, Mei Nabila Revina Kholifah, Siti Nurriyatul Kristianto, Sonny Kristina Handayani Kumalasari, Nurul Chamidah Lestari, Nasa Bela Dwi Maida, Margarita Claudya Mashudi Mashudi Maya Diva Maya Diva Siswidiani Muadifah, Afidatul Muadifah, Afidhatul Mutia Hariani Murjanah Mutia Hariani Nurjanah Nasa Bella Ngibad, Khoirul Niken Desi Wulandhari Nurin, Wachidatun Nurjanah, Mutia Hariani Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Parbuntari, Hesty Parbuntary, Hesty Putri, Amalia Eka Rahmadani, Anindita Mutiara Rahmawati, Ayu Sintya Citra Ramadhani, Melati Apriliana Retnosari, Rini Rian Anggia Wijaya Rury Eryna Putri Safitri, Yunita Diyah Sefrina Sefrina Siti Awwalul Amanatur Rohmah Siti Nurriyatul Kholifah Sri Wahyuningsih SULASTRI Sulastri Sulastri Tilarso, Dara Pranidya Tutik Dwi Wahyuningsih Ulayya, Nida Asma vifta, rissa laila Widayanti, Sindi Dwi Wimbuh Tri Widodo Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Wulandhari, Niken Desi Yuliandari, Fadhira Yulindasari, Armita Regina Putri