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Prayekti, Endah
Universitas Nahdlatul Ulama Surabaya
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Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli Endah Prayekti; Suliati Suliati; Dwi Agustin Wulandari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1906

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
Improving Public and Environmental Health Through Clean and Healthy Living Behavior Education and Bacterial Examination Endah Prayekti; Andreas Putro Ragil Santoso; Merry Sunaryo; Reski Novita Indaryanti; Lili Susan Rahmawati; Dina Kamelia
Indonesia Berdaya Vol. 7 No. 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261400

Abstract

A major problem frequently faced by the public is clean and healthy living behaviors (PHBS). According to the Indonesian Ministry of Health in 2014, health can be achieved by changing unhealthy behaviors into healthy ones and creating a health-supportive environment. Effective measures include handwashing with soap and environmental inspections. This activity aims to increase public awareness of the importance of clean and healthy living behavior (PHBS), while also introducing the dangers of microbiological contamination that can occur on hands and water. Community service activities are carried out through the stages of pre-test, education, post-test, sampling, and sample examination. Of the 34 respondents, the majority experienced a significant reduction in hand bacteria, with 21 showing a reduction in hand bacteria of more than 70% and some reaching 99–100%. Well water examinations at several public facilities for Pekingan village residents showed the presence of Coliform bacteria exceeding the safe limits according to the Ministry of Health Regulation, indicating that the water is not suitable for direct consumption due to possible contamination by domestic waste, septic tank seepage, or poor environmental sanitation. The conclusion of this Community Service activity is that there is an increase in respondents' understanding and an increase in their ability to practice proper handwashing. Water sanitation in public facilities needs to be considered and improved because it should not be used for consumption.
Improving Public and Environmental Health Through Rheumatoid Screening and Education on Environment Pathogenic Fungal Contamination Suprapto Maat; Endah Prayekti; Gilang Nugraha; Vania Nur Ramadani; Winda Melindah; Imraatul Mukarramah
Indonesia Berdaya Vol. 7 No. 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261411

Abstract

People working in agricultural environments with high humidity levels are at greater risk of exposure to pathogenic fungi, which can trigger infections of the skin, nails, and respiratory tract, especially in individuals with weakened immune systems. On the other hand, the elderly are vulnerable to health decline, including autoimmune diseases such as rheumatoid arthritis (RA), which can cause joint inflammation, tissue damage, and progressive disability. This community service activity aims to increase public knowledge about the dangers of pathogenic fungal contamination and to conduct early detection of RA through Rheumatoid Factor (RF) testing. Education was delivered interactively using lectures, discussions, and visual media, accompanied by pre- and post-tests, as well as RF testing using the latex agglutination method. This community service activity was attended by 33 participants. Results showed an increase in knowledge from 58% to 91% after the education, with all 33 RF test participants receiving non-reactive results. This program demonstrates that the combination of education and simple testing can increase public preventive awareness and is suitable for adaptation in other areas with similar risk characteristics.
Co-Authors Akbar Reza Muhammad Akhmad, Nur Faradila Althofur Hilman Rifki Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Arsya Tazkiya Asma' Denaya Psari Yuwono Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Brina Thursina Dibiasi Devita Rahma Putri Devyana Dyah Wulandari Dina Kamelia Dini Setiarsih dr Hartatiek Nila,Sp.OG Dwi Agustin Wulandari Elsya Octaviani Cantika Putri Endah Budi Permana Putri Ersalina Nidianti Eva Rosa Dwi Febrianti Evi Sylvia Awwalia Fadillah Triasmoro Fakurazi, Sharida Gilang Nugraha Gilang Nugraha Hamiduumajid Ballihg Ballihgoo Hamiduumajid Ballihg Ballihgoo Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Imraatul Mukarramah Indah Nur Kamaliyah Indaryanti, Reski Novita Kamelia, Dina Kardina, Rizki Nurmalya Khiliah Navis Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini Lili Susan Rahmawati Luqiana Fauziah M Nasir Maat, Suprapto Maki Zamzam Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Melindah, Winda Mia Puspitasari Mirshall Abiedama Prayoga Mochamad Faishal Riza Mohammad Dwinanda Junaedi Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Muhammad Taufiq Hidayat Mukarramah, Imraatul Mutiarawati, Diah Titik Nada Mufarrohah Nandasari, Anggy Dwipa Nastasya Nunki Nathalya Dwi Kartika Sari Navis, Khiliah Ngiluhtara Aditiya Putri Nisa, Fauziyatun Nur Naili Fahira Nur Sophia Matin Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Renny Novi Putri Lestari, Ajeng Anindhita Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qonita Iklila Maghfuroh Qotrinnada, Nabilah Rahmawati, Lili Susan Ramadani, Vania Nur Ratna Sofaria Munir Regina Ayu Fristiyanti Reski Novita Indaryanti Rhomadhoni, Muslikha Nourma Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Rizki Nurmalya Kardina Romdhoni, Muhammad Afwan Rosiana Dewi Regita Sa'bania Hari Raharjeng Sabrina, Shinta Milenia Sadza, Lailatus Salfa Salsabilah Zain Salimi, Syafrina Maulidyatus Salman Firmansyah Sari, Devi Fitriana Satya Nugraha Wirayudha Seggaf Achmad Syah Septina Isro’ Hermawati Setyana Yuli, Azizah Ari Siti Husnul Khotimah Sonia Fitriatul Qolbi Suliati Suliati Sunaryo, Merry Suprapto Maat Suryandaru, Suryandaru Syafiuddin, Achmad Tauziah, Amalia Thomas Sumarsono Thomas Sumarsono Thomas Sumarsono Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Vania Nur Ramadani Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Winda Melindah Wiwik Winarningsih Wulan, Wieke Sri Yauwan Tobing Lukiyono Yuliana Yuliana Yuni Nur Malita Sari Zahrotul Illiyin Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira