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Diamahesa, Wastu Ayu
Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Mataram
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Potensi Pemanfaatan Limbah Kulit Ari Kopi (Coffee Husk) dalam perikanan (Review) Diamahesa, Wastu Ayu; Muahiddah, Nuri
Journal of Fish Nutrition Vol. 3 No. 1 (2023): Journal Of Fish Nutrition
Publisher : Journal of Fish Nutrition

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfn.v3i1.2760

Abstract

The global demand for coffee is increasing each year, and as a result, the number of debris that will be generated will also be as large as coffee husks. Therefore, efforts must be made to employ coffee husks. In the disciplines of biogas, fertilizers, bioethanol, biofuels, enzymes, and bioactive compounds, efforts have been made to utilize coffee husks. However, only two researchers have investigated the use of coffee husks as an alternative raw material and preservative for tilapia flesh in the fisheries industry. This article explains the prospective use of coffee husks in the fisheries sector, particularly in fish farming and processing.
Identifikasi VNN dengan Metode PCR pada Ikan Kerapu Cantang (E. fuscoguttatus × E. lanceolatus): Studi Kasus di BPBAP Situbondo Dina Ahyani; Diamahesa, Wastu Ayu
Journal of Fish Nutrition Vol. 5 No. 1 (2025): Journal Of Fish Nutrition
Publisher : Journal of Fish Nutrition

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfn.v5i1.7280

Abstract

Viral Nervous Necrosis (VNN), caused by betanodavirus, poses a serious threat to the aquaculture of hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus), with mortality rates reaching 100% in larval and juvenile stages. This study aimed to develop a VNN detection protocol based on Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and nested PCR at the Brackishwater Aquaculture Development Center (BPBAP) in Situbondo, East Java, from February to April 2024. The methodology included necropsy for sampling brain and eye tissues, RNA extraction using a silica-based method, two-step amplification (RT-PCR and nested PCR), and visualization of results through 1.2% agarose gel electrophoresis. The findings revealed that 2 out of 10 hybrid grouper samples (20%) tested positive for VNN, with the detection of a specific 294 bp DNA band. The developed protocol demonstrated high sensitivity (CT values of 18–22 cycles) and optimal specificity, with no false-positive results. Temporal analysis suggested a periodic infection pattern with an 8-week interval. These results confirm the endemicity of VNN at BPBAP Situbondo and underscore the importance of early detection to prevent mass outbreaks. This study recommends implementing the protocol as part of a molecular-based disease surveillance system, along with further development using quantitative PCR (qPCR) for quantitative analysis. The findings are expected to support sustainable VNN control programs in hybrid grouper aquaculture.
PERAN BINDER DALAM FORMULASI PAKAN UNTUK BUDIDAYA KEPITING BAKAU (SCYLLA SPP.): KAJIAN BAHAN SINTETIS, ALAMI, DAN INOVATIF BERBASIS LINGKUNGAN Diamahesa, Wastu Ayu; Muahiddah, Nuri; Putri Lestari, Dewi
Journal of Fish Nutrition Vol. 5 No. 1 (2025): Journal Of Fish Nutrition
Publisher : Journal of Fish Nutrition

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfn.v5i1.7420

Abstract

The mangrove crab (Scylla spp.) is a high-value aquaculture commodity, where feed quality—particularly the binder component—significantly influences farming success. This article reviews the role of various binders in mangrove crab feed formulations, including synthetic binders such as carboxymethyl cellulose (CMC), natural binders (seaweed meal, agar, gelatin), and recent innovations such as fermented food waste and combinations with probiotics or black soldier fly larvae (BSFL) oil. Binders not only maintain feed stability in water but also affect growth, survival rate, feed efficiency (FCR), and immune response in crabs. Synthetic binders like CMC exhibit high stability (>24 hours) but are costly and less environmentally sustainable, whereas natural binders such as Gracilaria and agar offer a balance between stability, eco-friendliness, and additional nutritional benefits. The integration of binders with probiotics or BSFL oil has been shown to enhance immunity and growth performance. Binder selection should align with the farming phase—for instance, agar and gelatin are recommended for larval stages, while cornmeal and soy protein concentrate (SPC) are suitable for grow-out phases. This article also provides practical recommendations based on farming objectives, such as using natural binders to reduce water pollution or combining CMC with probiotics for intensive farming systems. This review is expected to serve as a guideline for farmers in selecting optimal binders to support efficient, cost-effective, and sustainable mangrove crab aquaculture.
Growth and Maintenance of Barramundi (Lates calcarifer) Fed with Commercial Feed at BPBAP Situbondo Andriana, Ina Eka; Diamahesa, Wastu Ayu
Jurnal Media Akuakultur Indonesia Vol 5 No 1 (2025): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v5i1.6916

Abstract

This study evaluated the growth performance and maintenance of white seabass (Lates calcarifer) fed with commercial feed at BPBAP Situbondo. As a high-value aquaculture commodity with increasing market demand, white seabass farming faces challenges in seed production and growth optimization. The research monitored growth parameters, survival rate (SR), and water quality during a 4-month rearing period (February-June 2024) in concrete tanks (2.5×4×1.2 m). Results showed that commercial feeds (Megami/Skretting) administered via ad-satiation method (3-4x/day for juveniles <7cm, 2x/day for 8-14cm fish) supported growth, though size variation triggered cannibalism. Water quality parameters remained optimal (pH 7.49-8.17; salinity 33-34‰; ammonia 0.011-0.026 mg/L) per SNI 8036:2014 standards, contributing to an 83.98% SR. Key findings emphasize the critical role of routine grading to prevent cannibalism, strict feed management to minimize toxic waste accumulation (TAN peaked at 1.397 mg/L), and daily water exchange (100% replacement). The study concludes that integrated feed-water management significantly enhances white seabass productivity, recommending further research on nutrient-specific feed formulations and advanced filtration systems to address the 16% mortality rate.
Comparative Analysis of Chlorella vulgaris Cell Densities in Different Culture Systems Shalehah, Atiatun; Diamahesa, Wastu Ayu
Jurnal Media Akuakultur Indonesia Vol 5 No 1 (2025): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v5i1.6920

Abstract

Chlorella vulgaris is a cosmopolitan microalga with high nutritional value, widely utilized in aquaculture, pharmaceuticals, and biofuel production. This study evaluated its growth performance across three culture scales (laboratory bottle, carboy, and intermediate concrete tank) at BPBAP Situbondo from February to June 2024. The research aimed to analyze the influence of scale on biomass productivity and identify critical environmental factors. Results demonstrated significant growth variations among scales. The laboratory bottle (5 L) achieved the highest cell density (54 × 10⁶ cells/mL) on day 11, attributed to optimal light distribution and stable temperature (30–33°C). Carboy cultures (10 L) showed moderate productivity (31 × 10⁶ cells/mL), while the intermediate scale (700–800 L) yielded the lowest density (6.8 × 10⁶ cells/mL) due to light limitation and suboptimal aeration. Water quality parameters remained stable across all systems, with pH 8.0, salinity 30–34 ppt, and uniform light intensity (500 lux). Statistical analysis confirmed significant differences in growth rates (p < 0.05), highlighting the inverse relationship between scale size and productivity. The study concludes that small-scale systems offer superior environmental control for C. vulgaris cultivation. For mass production, design improvements—such as enhanced aeration, closed photobioreactors, and artificial lighting—are recommended to replicate laboratory-scale efficiency. These findings provide actionable insights for optimizing microalgae culture systems in aquaculture feed production.
THE USE OF GARLIC (Allium sativum) AS AN IMMUNOSTIMULANT IN AQUACULTURE Muahiddah, Nuri; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 3 No. 1 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i1.2751

Abstract

The improvement of fish immunity is a critical factor in successful aquaculture. In recent years, the use of garlic (Allium sativum) as an immunostimulant has attracted researchers and farmers' interest in enhancing fish's health and disease resistance. This abstract discusses an overview of the use of garlic as an immunostimulant in aquaculture. Garlic is widely known for its antimicrobial, antioxidant, and immunostimulant properties. Several studies have evaluated the effects of garlic supplementation on various species of cultured fish. The results have shown that the administration of garlic in fish feed can enhance immune responses, such as increased immune enzyme activity and antibody production. Furthermore, active components in garlic, such as allicin and diallyl sulfide, have been proven effective against fish pathogens such as bacteria, viruses, and parasites. However, garlic as an immunostimulant in aquaculture still requires further research. Factors such as the appropriate dosage, interactions with farming conditions, and a deeper understanding of the underlying mechanisms must be considered. To optimise the use of garlic, further research is required to explore optimal dosages, interactions with environmental factors, and a better understanding of its effects on fish immune responses. In conclusion, garlic as an immunostimulant in aquaculture shows promising potential. The oral administration of garlic extract through the fish feed is capable of preventing infections by Aeromonas sp. and Aeromonas hydrophila in fish species such as Tawes, Catfish, Dumbo catfish, and Nile tilapia, while also enhancing non-specific immunity, such as increased total hematocrit, total haemoglobin, total erythrocytes, and phagocytic activity.
THE USE OF IMMUNOSTIMULANTS FROM PAPAYA LEAVES TO TREAT DISEASE AND INCREASE NON-SPECIFIC IMMUNITY IN FISH AND SHRIMP Muahiddah, Nuri; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 3 No. 1 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i1.2755

Abstract

The use of immunostimulants from papaya leaves as a treatment strategy for fish diseases has gained attention in the field of aquaculture. Initial studies have shown that the active compounds in papaya leaves have the potential as immunostimulants to enhance the fish immune system and combat disease infections. Previous studies have revealed that the administration of papaya leaf extracts in fish feed can stimulate immune responses, including activating immune cells such as macrophages and increasing antibody production. The compounds in papaya leaves also exhibit antimicrobial effects that help fight against pathogens causing diseases in fish. Although research findings indicate the potential use of papaya leaf immunostimulants, further studies are needed to understand their mechanisms of action comprehensively. Factors such as effective dosage, optimal administration methods, and their effects on various diseases must be clarified. Safety aspects should also be considered, including potential side effects and residues in fish intended for human consumption. Consideration should also be given to sustainable fish farming practices and the potential environmental impacts of the widespread use of papaya leaf immunostimulants. In conclusion, using immunostimulants from papaya leaves to treat fish diseases shows promising potential. However, further research is required to understand their effects thoroughly, determine the appropriate dosage, evaluate the safety, and consider sustainable aquaculture aspects before their use can be widely recommended in aquaculture practices.
Evaluation of Milkfish (Chanos chanos) Breeding Activities at the Balai Besar Perikanan Budidaya Air Payau (BBPBAP) jepara Maudina, Finda; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 3 No. 2 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i2.3748

Abstract

Cultivation of milkfish in Indonesia plays a central role in the fisheries sector, covering an expansive area of approximately 600,000 hectares and achieving a production of 621,393 tons in 2014. Milkfish-producing regions are distributed across various areas, including Sulawesi, Kalimantan, and Java, employing diverse cultivation technologies. Milkfish holds a crucial role in meeting food needs and contributing to the economic well-being of communities. Despite the predominantly traditional cultivation practices, yielding between 500 kg to 1 ton per hectare per year, there are also semi-intensive cultivation methods with yields reaching 3 tons per hectare per year. The high demand for milkfish seeds necessitates optimal water quality and strict supervision, serving as decisive factors in attaining optimal production outcomes. This research aims to acquire knowledge and skills related to milkfish breeding techniques, particularly at the Balai Besar Perikanan Budidaya Air Payau (BBPBAP) in Jepara. Data collection methods involve observation, active participation, and direct interviews. The results of this study indicate that the management of feed, broodstock maintenance, and feed management in milkfish breeding at BBPBAP Jepara are well-executed, supporting the success of milkfish breeding. The implications of this research can contribute positively to the development of milkfish cultivation techniques, focusing on milkfish breeding to achieve optimal production outcomes.
Evaluation of Common Carp (Cyprinus carpio) Cultivation Techniques at Balai Benih Ikan (BBI) Lingsar, West Nusa Tenggara Aeni, Nurul; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 4 No. 1 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i1.4416

Abstract

This research aims to evaluate the cultivation techniques of common carp (Cyprinus carpio) at the Balai Benih Ikan Lingsar Installation, West Nusa Tenggara. The research method involved observation, interviews, and common carp cultivation activities. Data collected included pond preparation, seed selection and stocking, feed management, carp seed growth, evaluation of survival rate (SR), feed conversion ratio (FCR), and water quality measurement. The research results showed significant growth in common carp seeds, with average weight and length increasing from the beginning to the end of the rearing period. The survival rate (SR) reached 97%, while the feed conversion ratio (FCR) reached 0.77, indicating good feed efficiency. Water quality during the common carp cultivation period was within the optimal range, with pH, dissolved oxygen (DO), and temperature values suitable for fish growth. This research demonstrates that the common carp cultivation techniques applied at the Balai Benih Ikan Lingsar Installation are effective in supporting the growth and survival of common carp.
Natural Feed Administration During Fattening Stage for the Growth of Mud Crabs (Scylla sp.) Under Different Rearing Systems Rahmadani, Thoy Batun Citra; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 4 No. 4 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i4.5930

Abstract

Mud crab (Scylla spp.) aquaculture holds significant potential in supporting food security, economic growth, and ecological balance. This study aims to summarize various findings related to critical factors in mud crab farming, such as feed types, rearing systems, and stocking density, and their impact on productivity, feed conversion ratio (FCR), and economic profitability. The review highlights that trash fish feed at 15% of body weight yields optimal growth and feed efficiency due to its high protein content. Feed diversification, including stingray and janitor fish, offers promising results with reduced costs. Various rearing systems are employed, ranging from cages and earthen ponds to advanced technologies such as Recirculating Aquaculture Systems (RAS). Small bamboo floating cages demonstrate superior outcomes in growth and survival rates (SR). Mangrove habitats significantly enhance crab growth, while silvofishery systems provide a sustainable farming alternative. Optimal stocking density, such as 4 crabs/m², balances growth and production. Additionally, Scylla serrata exhibits the best growth rate compared to other species, particularly males. Innovations like soft-shell farming technology add economic value to the product. Overall, the success of mud crab aquaculture depends on the optimal combination of feed, rearing systems, and environmental management. This review offers strategic insights for developing efficient, sustainable, and economically viable crab farming practices.
Co-Authors Abidin, Z Adriana Monica Sahidu Agustin, Pradita Ayu Ahyani, Dina Aini, Nila Robiatul Alis Mukhlis Andre Rachmat Scabra Andriana, Ina Eka Angga, Prayogi Dwina Asri, Yuliana Atiatun Shalehah Aula Husnul Fajri Badrun Badrun Badrun Bagus Dwi Hari Setyono Bagus Dwi Hari Setyono Batun Citra Rahmadani, Thoy Chandrika Eka Larasati, Chandrika Eka Chen, Peter Damai Diniariwisan Damai Diniariwisan Damai Diniariwisan DEDI JUSADI Devi Wahyuni Cahyani Dewi Putri Lestari Dewi Putri Lestari, Dewi Putri Dina Ahyani Dina Ahyani Diniariwisan, Damai Dwi Budi Santoso Dwina Angga, Prayogi Dwiyanti, Septiana Fadliana Khofiatun Fariq Azhar Fitriani, Laily Fitriani Hadzriil Jeeffry , Ku Haini, Naufara Hak, Muhammad Bai`ul Hizbulloh, Lalu Idris Affandi, Rangga Ina Eka Andriana Juli Ekasari Kaswadi, Hendri Khofiatun, Fadliana Laily Fitriani Laily Fitriani Mulyani Laily Fitriani Mulyani Lalu Hizbulloh Lumbessy, Salnida Yuniarti Masumoto, Toshiro Maudina, Finda Mia Setiawati Miranti, Sevia MUHAMMAD AGUS SUPRAYUDI Muhammad Amin Muhammad Junaidi Muhammad Junaidi Muhammad Marzuki Muhammad Sumsanto Mulyani, Laily Fitriani Mutiara, Ni Luh Nanda Diniarti Naufara Haini Nisa, Haerunnisaa Nunik Cokrowati Nunik Cokrowati Nuri Muahiddah Nurul Aeni Panosa, Alief Erwanda Pebrianti, Ni Luh Mutiara Putri Lestari, Dewi Rahmadani, Thoy Bathun Citra Rahmadani, Thoy Batun Citra Rahmadhani, Thoy Batun Citra Ramdani, Nursuci Rangga Idris Affandi Retno Palupi RR. Ella Evrita Hestiandari Sadikin Amir Sahrul Alim Sapto Andriyono Septiana Dwiyanti Septiana Dwiyanti Septiana Dwiyanti Septiana Dwiyanti Septiana Dwiyanti Sevia Miranti Shalehah, Atiatun Sitti Hilyana Syawalina Fitria Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Toshiro Masumoto Toshiro Masumoto Widia Lestari Windi Yuastika Yuastika, Windi Yuliana Asri Yuliana Asri Yuliana Asri Yuliana Asri Yuliana Asri YUSRIN YUSRIN Zaenal Abidin