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Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/628595

Abstract

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 μl, 15 μl, and 10 μl. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 μl is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.
Pengaruh Penyebaran Berita Hoaks Covid-19 Terhadap Prilaku Pengguna Media Sosial Facebook (Studi Kasus Pada Desa Pugul Kecamata Riau Silip Kabupaten Bangka): Indonesia Seprianto, Seprianto
Jurnal Ilmu Komunikasi Vol 5 No 1 (2022): Jurnal Ilmu Komunikasi
Publisher : Pahlawan 12 Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47995/jik.v5i1.81

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penyebaran berita hoaks covid-19 terhadap perilaku pengguna media sosial facebook di Desa Pugul Kecamatan Riau Silip. Penelitian ini merupakan penelitian kuantitatif dengan dengan metode korelasi, korelasi yang digunakan korelasi asosiatif penelitian ini dilakukan di Desa Pugul Kecamatan Riau silip Kabupaten Bangka, responden dalam penelitian ini ialah pengguna media sosial facebook. Teknik pengumpulan data menggunakan kusioner dan dokumentasi. Hasil penelitian menunjukan bahwa terdapat pengaruh penyebaran berita hoaks terhadap perilaku pengguna media sosial facebook. Besarnya korelasi antara variabel bebas berita hoaks terhadap variabel terikat perilaku pengguna media sosial facebook yang ditunjukan dengan angka korelasi 0.213 menunjukan adanya hubungan yang “rendah”. Hasil analisis koefisien determinasi diketahui besarnya kontribusi variabel x terhadap variabel y yang ditunjukan oleh R square sebesar 0.045 angka ini menunjukan bahwa variabel X yang digunakan dalam persamaan regresi ini mampu memberikan kontribusi terhadap variabel Y sebesar 4.5% sedangkan sisanya 95.5% dipengaruhi oleh faktor lain selain berita hoaks.
EKSPLORASI EMOSI DALAM PEMBELAJARAN FISIKA DI SEKOLAH MENENGAH ATAS: STUDI DESKRIPTIF DAN PERBANDINGAN ANTAR TINGKATAN KELAS Seprianto, Seprianto; Pranata, Ogi Danika; Juniyati, Sintia; Susanti, Sela
DIKSAINS : Jurnal Ilmiah Pendidikan Sains Vol. 4 No. 2: Juni 2024
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/diksains.4.2.101-108

Abstract

ABSTRAK   Penelitian ini bertujuan untuk mengeksplor tingkat emosi negatif pelajar dalam pembelajaran fisika di tingkat Sekolah Menengah Atas dan membandingkannya antara tingkatan kelas yang berbeda. Metode kuantitatif deskriptif dan komparatif digunakan dengan melibatkan 60 pelajar dari tiga tingkatan kelas yang berbeda. Data dikumpulkan menggunakan kuesioner Achievement Emotions Questionnaire (AEQ) dan dianalisis menggunakan statistik deskriptif serta uji ANOVA (Analysis of Variance). Hasil menunjukkan bahwa mayoritas pelajar mengalami tingkat emosi negatif pada kategori sedang, dengan beberapa faktor penyebab seperti perasaan malu atau takut untuk berbicara di kelas dan kecemasan saat tidak memahami materi. Meskipun terdapat perbedaan rata-rata tingkat emosi negatif antar kelas, namun tidak ditemukan perbedaan yang signifikan secara statistik. Implikasi praktis dari penelitian ini adalah bahwa guru dapat menggunakan temuan ini sebagai dasar untuk merancang strategi pembelajaran yang lebih adaptif, sementara implikasi teoritisnya adalah kontribusinya terhadap pengembangan teori-teori yang berkaitan dengan pengaruh emosi dalam pembelajaran fisika. Penelitian selanjutnya disarankan untuk memperluas cakupan penelitian dengan mempertimbangkan faktor-faktor lingkungan dan personal yang mempengaruhi emosi pelajar serta menggunakan metode pengukuran emosi yang lebih holistik dan valid.   Kata  kunci—Emosi, Fisika, Pembelajaran, Pelajar, Komparasi   ABSTRACT   This research aims to explore the level of negative emotions among high school students in physics learning and compare them across different class levels. A descriptive and comparative quantitative methods was employed, involving 60 students from three different class levels. Data were collected using the Achievement Emotions Questionnaire (AEQ) and analyzed using descriptive statistics and ANOVA (Analysis of Variance) tests. The results indicate that the majority of students experienced moderate levels of negative emotions, with contributing factors such as feeling ashamed or fearful of speaking in class and anxiety when not understanding the material. Although there were differences in the average levels of negative emotions among classes, no statistically significant differences were found. The practical implications of this research are that teachers can use these findings as a basis for designing more adaptive teaching strategies, while the theoretical implications lie in its contribution to the development of theories related to the influence of emotions on physics learning. Further research is suggested to broaden the scope by considering environmental and personal factors influencing student emotions and using more holistic and valid emotion measurement methods   Keywords—Emotion, Learning, Physics, Students, Comparative
Sosialisasi Integrasi Teknologi dalam Pembelajaran Sains Menggunakan Physics Education Technology (PhET) Pranata, Ogi Danika; Seprianto, Seprianto; Adelia, Ismi; Darwata, Siti Riva
Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol 11 No 2 (2024): Mei
Publisher : Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/ppkm.v11i2.6707

Abstract

The community engagement activity was conducted using the community-based research method. The engagement began with the formation of a community and continued with socialization activities and the application of technology integration in science education. Finally, an evaluation was conducted in the form of concept comprehension tests and student perception surveys. The activities were implemented according to plan and achieved the desired goals. Firstly, students' conceptual understanding surpassed the minimum criteria (70.36). However, difficulties were still encountered by students related to concept comprehension. These difficulties need to be further investigated. Secondly, students' perception of the activities was considered excellent (84.00). They acknowledged that integrating technology using PhET could create engaging learning experiences, enhance motivation to learn, assist in understanding concepts, and encourage them to actively engage in learning. However, they still doubted whether PhET could be as beneficial as they perceived it to be in other science topics. Thus, learning activities with PhET integration are deemed suitable and evaluated for their usefulness in other science topics.
PENGARUH KONFLIK PERAN GANDA DAN STRESS KERJA TERHADAP KINERJA DOSEN WANITA SE PTKIN PROVINSI Seprianto, Seprianto; Daflaini, Daflaini
Jurnal Dedikasi Pendidikan Vol 9, No 1 (2025): Januari 2025
Publisher : Center for Research and Community Service (LPPM) University of Abulyatama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30601/dedikasi.v9i1.5691

Abstract

This study aims to determine whether there is an influence of Work Family conflict and work stress on the performance of lecturers at Stete Islamic Institute of Kerinci and State Islamic University of Sulthan Thaha Saefuddin Jambi. The sample of this study were female lecturers at the Faculty of Education and Teacher Training at State Islamic Institute of Kerinci and State Islamic University of Sulthan Thaha Saifuddin Jambi which consisted of 98 lectures, and this was used total sampling technique. The data collection techniques used a questionnaire. Then, the Data was analysed by using SPSS 21 program which used multiple linear analysis. The results of this study showed that (1) there is a positive and significant influence between Work Family conflict on the performance of female lecturers at State Islamic Institute of Kerinci and State Islamic University of Sulthan Thaha Saefuddin Jambi with a significance value of 0.000 0.05, (3) there is a Work Family conflict and work stress has a positive and significant influence on the performance of female lecturers at State Islamic Institute of Kerinci and State Islamic University of Sulthan Thaha Saifuddin Jambi with a significance value of 0.000 < 0.05.
PENINGKATAN PENGETAHUAN TENTANG DIABETES MELLITUS MELALUI KULIAH TAMU PADA MAHASISWA IIUM MALAISYA Adri Nora; Seprianto Sepriant
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 3: Agustus 2022
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i3.2771

Abstract

Diabetes Milletus merupakan salah penyakit yang cukup banyak diderita oleh masyarakat di dunia. Diabetes sendiri adalah penyakit kronis yang memiliki gejala dengan tingginya kadar gula (glukosa) di dalam darah. Asia Tenggara merupakan salah satu wilayah yang memiliki lebih dari 75 juta orang penderita diabetes. Malaisya merupakan salah negara yang terus berkembang secara ekonomi dan kultural, dimana dengan adanya perkembangan tersebut membuat penyakit diabetes dan penyakit jantung lainnya ikut berkembang di negara ini. Universitas Esa Unggul bekerjasama dengan IIUM mengadakan program kuliah tamu untuk meningkatkan edukasi tentang penyakit diabetes mellitus melalui perkuliahan Biokimia medisinal. Kegiatan kuliah tamu ini dilaksanakan secara daring melalui media zoom dalam beberapa kali pertemuan. Sebanyak 24 mahasiswa peserta kuliah tamu biokimia medisinal mengikuti kegiatan ini. Kegiatan dimulai dengan pemaparan topik, tanya jawab, dan ditutup dengan diskusi dua arah. Di akhir kuliah tamu, mahasiswa diberikan link untuk memberikan feedback terhadap topik dan suasana perkuliahan. Hasil feedback yang diberikan adalah semua mahasiswa puas dengan topik kuliah tamu dan juga puas terhadap pemaparan yang dilakukan oleh dosen dari Esa Unggul. Mereka juga sangat senang atas diskusi yang menarik dan juga berterima kasih atas ilmu baru yang diberikan
WORKSHOP ISOLASI DNA DAN PENGENALAN ALAT LABORATORIUM BIOTEKNOLOGI BAGI GURU BIOLOGI SMA/MA SE JAKARTA Seprianto Seprianto; Henny Saraswati; Febriana Dwi Wahyuni; Titta Novianti; Adri Nora; Aroem Naroeni; Putri Handayani
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 3: Agustus 2022
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i3.2780

Abstract

Pengembangan bioteknologi dalam pengelolaan sumberdaya hayati dapat menjadi potensi terbesar dalam bidang biodiversitas dan menjadi aset nyata untuk kesejahteraan masyarakat. Bioteknologi didukung dengan metode – metode mutakhir serta fasilitas laboratorium yang memadai untuk mewujudkan keilmuan yang mampu dipahami oleh para siswa dengan transfer ilmu yang baik dari seorang guru biologi. Guru yang menguasai materi pelajaran dengan baik, menggunakan metode dan media pembelajaran yang tepat, mampu mengelola kelas dengan baik akan memberi pengaruh yang positif terhadap prestasi belajar siswa. Pengabdian ini bertujuan untuk menambah wawasan materi tentang DNA, mampu melakukan isolasi DNA dengan benar serta mengenal jenis alat – alat laboratorium yang digunakan untuk mengisolasi dan visualisasi DNA. Peserta dihadiri sebanyak 23 orang yang tersebar dari berbagai SMAN/SMAS se Jakarta. Pelaksanan pengabdian dilakukan dengan 2 metode yaitu klasikal dan praktek di laboratorium. Kegiatan berjalan dengan baik dan lancar. Evaluasi kegiatan dilihat dari hasil kuisioner yang diisi oleh peserta yang merasa sangat berkesan dan bermanfaat dalam menjelaskan materi bioteknologi. Harapan kedepan dari peserta adanya pelatihan yang lebih advance dalam bidang bioteknologi seperti kultur jaringan, teknologi DNA rekombinan, stem cell, kloning gen dan kultur mikroba di Laboratorium.
PEMANFAATAN LIMBAH CANGKANG RAJUNGAN MENJADI KITIN DAN KITOSAN PADA UMKM RAJUNGAN KUPAS KOTA LANGSA Seprianto, Seprianto; Alham, Fiddini; Mahyuny, Siska Rita; Komariyah, Siti
Jurnal Abdi Insani Vol 12 No 5 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i5.1640

Abstract

Kuala Langsa Village, located in the city of Langsa, is a coastal area where the majority of the population works as fishermen, primarily catching blue swimming crabs. The local MSME (Micro, Small, and Medium Enterprise), UD Langsa Jaya, produces a significant amount of crab shell waste, which has so far been discarded into the sea. This waste can actually be processed to produce chitin and chitosan—both of which are valuable and environmentally friendly materials. However, the partner lacks the knowledge, skills, and tools necessary to manage this waste effectively. The objective of this program is to enhance the partner's knowledge and skills in processing crab shell waste into chitin and chitosan, which are both economically valuable and eco-friendly products. The methods used in this program include planning, preparation, implementation, and monitoring and evaluation. Additionally, the activities involve identifying the partner's problems, socializing the benefits of crab shell waste utilization, conducting training on chitin and chitosan production, and carrying out continuous evaluations of each stage. At UD Langsa Jaya, the training successfully taught five female workers how to transform crab shell waste into chitin and chitosan. They learned about waste processing and the economic and environmental benefits of the resulting products. This training not only improved the participants' skills but also helped reduce marine pollution. The use of chitosan as a natural preservative can also lower production costs for UD Langsa Jaya and make its products more competitive. Overall, this activity enhanced the partner’s knowledge and skills in processing crab shell waste into chitin and chitosan that are both economically valuable and environmentally sustainable.
Pelatihan dan Pembelajaran Tumbuhan Melalui Herbarium Kering di Kelas 5 SD Negeri Patung Oksal, Efriyana; Wiriatmaja, Nuraini Ulya; Lawrence, Yoel; Zadrax, Adellino; Seda, Ananda Putri; Situmorang, Larissa Kristina T.T.; Sidauruk, Christina; Sianturi, Dody Jhonatan Wilfiez; Manalu, Mely Grace Manalu; Riansyah, Irza; Kibtiyah, Maryatul; Gloria, Meilina; Ayu, Yulia Agustina; Maulida, Maulida; Seprianto, Seprianto
Journal of Appropriate Technology for Community Services Vol. 6 No. 2 (2025)
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jattec.vol6.iss2.art4

Abstract

This service aims to increase the understanding of grade 5 students at SD Negeri Patung about plant diversity through training and introduction to the herbarium. The problem faced is students' lack of knowledge about plant types and the importance of conservation. The methods used include the first stage of socialization of herbarium activities and the second stage of dry herbarium training. The results of the service show an increase in student's knowledge about plant classification, as well as skills in collecting and preserving plant specimens. As many as 80% of students showed high enthusiasm and were able to explain the process of making a herbarium well. In conclusion, this training not only increases students' knowledge about plants but also fosters a sense of love for the environment. The use of herbarium as a learning tool has proven effective in introducing the concept of biodiversity to the younger generation. It is hoped that this program can be implemented on an ongoing basis to support environmental education in elementary schools.
PENGEMBANGAN MEDIA PEMBELAJARAN BERBASIS ADOBE FLASH CS6 PADA MATERI KONFIGURASI ELEKTRON Erani, Friska; Seprianto, Seprianto; Prasetya, Cyndi
KATALIS: Jurnal Penelitian Kimia dan Pendidikan Kimia Vol 8 No 1 (2025): Jurnal Katalis Vol. 8 No. 1 Tahun 2025
Publisher : Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/katalis.v8i1.10720

Abstract

During the learning process, teachers only use lecture methods and rarely use learning media. This is what makes students feel bored and consider chemistry learning very difficult to learn. Therefore, to increase students' interest in learning, learning media is needed, one of which is interactive learning media based on Adobe Flash CS6. This study aims to determine the feasibility, practicality and attractiveness of the Adobe Flash CS6-based learning media that was developed. This study uses the R&D (Research and Development) method with the ADDIE model which is limited to the Development stage. The instruments used are needs analysis questionnaires, validation sheets of material experts and media experts, practicality questionnaires, and media attractiveness questionnaires. The subjects of the study consisted of 4 chemistry teachers at State Senior High Schools in Langsa City and 90 students. The results of the study indicate that chemistry teachers need android-based learning media on electron configuration material, which is indicated by the results of the material validation feasibility questionnaire obtained an Aiken'V value of 0.879 and the media obtained an Aiken'V value of 0.926 with very valid criteria, the level of practicality obtained 90.41% with very practical criteria and the attractiveness of the media obtained a value of 81.85% with very interesting criteria. Based on the results of the study, it was concluded that Adobe Flash CS6-based learning media is feasible to be used as a learning medium for electron configuration material.Keywords:, Learning Media, Adobe Flash CS6, Electron Configuration