Article Per Year (5 Year)
p-Index From 2021 - 2026
6.586
P-Index
This Author published in this journals
All Journal The Indonesian Biomedical Journal Syntax Literate: Jurnal Ilmiah Indonesia Alami Journal (Alauddin Islamic Medical) Journal MITRA: Jurnal Pemberdayaan Masyarakat Bioscientia Medicina : Journal of Biomedicine and Translational Research JURNAL MUTIARA KESEHATAN MASYARAKAT Syntax Idea An-Nadaa: Jurnal Kesehatan Masyarakat Jurnal Bakti Masyarakat Indonesia Jurnal Scientia Jurnal Kesehatan Tambusai Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Pengabdian Kepada Masyarakat Sisthana Prosiding Seri Seminar Nasional Jurnal Suara Pengabdian 45 Jurnal Riset Rumpun Ilmu Kedokteran (JURRIKE) Tarumanagara Medical Journal Sewagati: Jurnal Pengabdian Masyarakat Indonesia Cakrawala: Jurnal Pengabdian Masyarakat Global Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran Jurnal Keperawatan Bunda Delima Jurnal Pengabdian Masyarakat Bunda Delima Ebers Papyrus Journal of Community Service and Society Empowerment Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia Jurnal Pengabdian Masyarakat Jurnal Pengabdian Bidang Kesehatan Science and Technology: Jurnal Pengabdian Masyarakat Health Community Service Gotong Royong Prosiding Seminar Nasional COSMIC Kedokteran ALMURTADO: Journal of Social Innovation and Community Service Jurnal Serina Abdimas Jurnal Pengabdian Ilmu Kesehatan
Triyana Sari
Fakultas Kedokteran Universitas Tarumanagara
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

Published : 53 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Scientia

 1 
Development of Amplification Refractory Mutation System PCR to Detect Androgen Receptor Gene G1773A (rs6152) Polymorphism Triyana Sari; Erick Sidarta; Sari Mariyati Dewi Nataprawira; Giovani Evangelista Alfonsius; Damar Sajiwo
Jurnal Scientia Vol. 12 No. 03 (2023): Education, Sosial science and Planning technique, 2023 (June-August)
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58471/scientia.v12i03.1694

Abstract

Androgen receptor (AR) is a ubiquitous receptor responsible for responses by androgen stimulus. Androgen, a hormone which will bind to the AR, is essentials for normal male sexual development. Nevertheless, one of the polymorphisms in the AR gene, G1733A (rs6152) have been associated with numerous clinical risks such as cardiovascular disease (CD), androgenetic alopecia, high prostate-specific antigen (PSA) levels, male infertility, recurrent spontaneous abortions and prostate cancer. This study aims to develop an alternative and cost-effective method to detect G1773A (rs6152) polymorphism. In this study, amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) using two pair of primers was used to detect the G1733A (rs6152) polymorphism. Primer design was done using http://primer1.soton.ac.uk/primer1.html online tools and then manually adjusted to increase the specificity. A total of 54 samples were screened using ARMS PCR and 2 representative samples of each allele from previous screening were used to validate the results using Sanger DNA sequencing. Among 54 subjects screened, we found 52 (96.3%) subjects carry G allele and 2 (3.7%) subjects carry A allele. No heterozygote was found in this study. The frequency of G allele was 96.97% and the frequency of A allele was 3.03%. Result validation using DNA sequencing was in agreement with ARMS-PCR method results. ARMS-PCR can be used as efficient alternative for genotyping of G1733A (rs6152) AR gene polymorphism.
DEVELOPMENT OF AMPLIFICATION REFRACTORY MUTATION SYSTEM PCR TO DETECT ANDROGEN RECEPTOR GENE G1773A (RS6152) POLYMORPHISM Triyana Sari; Erick Sidarta; Sari Mariyati Dewi Nataprawira; Giovani Evangelista Alfonsius; Damar Sajiwo
Jurnal Scientia Vol. 12 No. 03 (2023): Education, Sosial science and Planning technique, 2023 (June-August)
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Androgen receptor (AR) is a ubiquitous receptor responsible for responses by androgen stimulus. Androgen, a hormone which will bind to the AR, is essentials for normal male sexual development. Nevertheless, one of the polymorphisms in the AR gene, G1733A (rs6152) have been associated with numerous clinical risks such as cardiovascular disease (CD), androgenetic alopecia, high prostate-specific antigen (PSA) levels, male infertility, recurrent spontaneous abortions and prostate cancer. This study aims to develop an alternative and cost-effective method to detect G1773A (rs6152) polymorphism. In this study, amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) using two pair of primers was used to detect the G1733A (rs6152) polymorphism. Primer design was done using http://primer1.soton.ac.uk/primer1.html online tools and then manually adjusted to increase the specificity. A total of 54 samples were screened using ARMS PCR and 2 representative samples of each allele from previous screening were used to validate the results using Sanger DNA sequencing. Among 54 subjects screened, we found 52 (96.3%) subjects carry G allele and 2 (3.7%) subjects carry A allele. No heterozygote was found in this study. The frequency of G allele was 96.97% and the frequency of A allele was 3.03%. Result validation using DNA sequencing was in agreement with ARMS-PCR method results. ARMS-PCR can be used as efficient alternative for genotyping of G1733A (rs6152) AR gene polymorphism
Co-Authors Alexander Halim Santoso Alexander Halim Santoso Alfianto Martin Alifia, Tosya Putri Alvianto, Fidelia Alya Dwiana Alya Dwiana Alya Dwiana Andianto, Ivan Christian Arlends Chris Averina, Friliesa Brian Albert Gaofman Bruce Edbert Bryan Anna Wijaya Chairunnisa Savitri Damar Sajiwo Damar Sajiwo Daniel Goh David Limanan Dean Ascha Wijaya DENY KURNIAWAN Destra, Edwin Dewi, Andriana Kumala Dorna Y.L. Silaban Edbert, Bruce Edwin Destra Enike, Syilvia Cendy Erick Sidarta Erick Sidharta Fadilah, Diana Farell Christian Gunaidi Farell Christian Gunaidi Fernando Nathaniel Fiona Valencia Setiawan Fladys Jashinta Mashadi Gabriella, Regina Gaofman, Brian Albert Geoffrey Christian Lo Giovani Evangelista Alfonsius Giovani Evangelista Alfonsius Giovanno Sebastian Yogie Goh, Daniel Gracienne Gracienne Gunaidi, Farell Christian Hartono, Vincent Aditya Budi I Made Satya Pramana Jaya Ivan Christian Andianto Jap, Ayleen Nathalie Johny Hartawan Sutrisno Joshua Kurniawan Kenzie Rafif Ramadhani Khoto, Anthon Eka Prayoga Kosasih, Robert Kristianto, Angeline Florencia Grace Kurniawan, Joshua Laura, Gabrielle Lucius, Steven Hizkia Lumintang, Valentino Gilbert Mashadi, Fladys Jashinta Meilani Kumala Muhammad Rifat Umar Alwini Najwa Anindya Nataprawira, Sari Mariyati Dewi Normala, Ajeng Olivia Charissa Permatasari, Nabila Jingga Peter Ian Limas Pratama, Dena Adistiya Rambe, Novitasari Reynardo, Reynardo Ribka Anggeline Hariesti Sajiwo, Damar Santoso, Alexander Halim Sara Jayanti Sari Mariyati Dewi Nataprawira Sari Mariyati Dewi Nataprawira Sari Mariyati Dewi Nataprawira Sari Mariyati Dewi Nataprawira Sari Mariyati Dewi Nataprawira Sari, Fadhilah Permata Satyanegara, William Gilbert Satyo, Yovian Timothy Savitry, Chairun Nisa Setiawan, Fiona Valencia Shirly Gunawan Sidarta, Erick Sidharta, Erik Silvia W. Lestari Sitorus, Ribka Angeline Hariesti Sugiharto, Hans Sukianto, Louise Audrey Sukmawati Tansil Tan Susilodinata Halim Sutrisno, Johny Hartawan Syarifah, Andini Ghina Teguh, Stanislas Kotska Marvel Mayello Tiara Rahmananda Valentino Gilbert Lumintang Wijaya, Bryan Anna Wijaya, Christian William Gilbert Satyanegara Windiatmoko, Dhio Dwi Yani, Jovan Falian Yohanes Firmansyah Yovian Timothy Satyo