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All Journal Buletin Peternakan METAMORFOSA Journal of Biological Sciences Biotropika Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biodjati BIOEDUSCIENCE Jurnal Biologi Tropis Justek : Jurnal Sains Dan Teknologi Biosel: Biologi Science and Education Jurnal Pengabdian Magister Pendidikan IPA Biota BIO-EDU: Jurnal Pendidikan Biologi Jurnal Abdidas Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi Media Eksakta Journal Evaluation in Education (JEE) AGRI-TEK : Jurnal Penelitian Ilmu-ilmu Eksakta Interdisciplinary International Journal of Conservation and Culture Journal of Biology Science and Education
Manap Trianto
Departement Of Biology Education, Faculty Of Teacher Training And Education, Tadulako University
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Buletin Peternakan

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Molecular Phylogeny of Stingless Bees in the Special Region of Yogyakarta Revealed Using Partial 16S rRNA Mitochondrial Gene Manap Trianto; Hari Purwanto
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.55539

Abstract

Systematics of bees has developed rapidly. Several studies have attempted to infer the kinship between each group of bee. One way is the use of phylogenetic analysis using molecular data. This study explains the phylogenetic relationship of stingless bees in the Special Region of Yogyakarta, Indonesia based on the 16S rRNA gene. The research has been carried out in five districts and cities in the region from June to September 2019. In the study, the stages implemented include; place determination of stingless bees sampling, sampling, and molecular identification (DNA extraction, DNA amplification, and sequencing), followed by the data analysis using NCBI database and MEGA X software. The result of this study indicated that among seven morphospecies from Yogyakarta, there are six species of stingless bees that have a closest genetic relationship with the same species data from Genbank, namely Tetragonula laeviceps, T. iridipennis, T. sapiens, T. sarawakensis, Lepidotrigona terminata, and Heterotrigona itama. Since the genetic distances of T. laeviceps, T. iridipennis, T. sapiens, are wider than 3,5%, the data indicate that there is a possibility that the three morphospecies are actually belong to a different species with a similar morphology. Meanwhile, for the morphospecies T. biroi, the closest hit is on T. pagdeni 16S rRNA DNA because the T. biroi 16S rRNA DNA data is not available on the database. This study is expected to contribute to the preservation and utilization of one of Indonesia's important biodiversity resources.
Co-Authors Abd Rauf Abd. Rauf Abia, Hikma Achmad Ramadhan Achmad Ramadhan Adnan, Bahana Aditya Agung Dwi Kurniawan Ahmad Ramadhan Akram, Akram Alimudin, Sukmawati Amalia Buntu Amiruddin Kasim Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Astija Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delima, Nur Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham Efendi EFENDI Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatma Dhafir Fatmah Dhafir Febriani, Vita Indri Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Isninar, Isnainar Ivan Permana Putra Kaini, Kaini Kaltisa, Kaltisa Khaerunnisa Kundera, I Negah Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah M. Abimanyu Marisa, Fajri Masriani . Masrianih Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh. Dahri Kisman Mohammad Jamhari Mursito S. Bialangi Ni Gusti Ayu Galuh Candra Kirana Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi Raya Agni Ritman Ishak Paudi Rizaldy Pratama Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Saliyem, Saliyem Samsurizal, M. Sulaeman Saridewi, Uut Sitti Nur Fadhilah Sri Hardianti Sukmawati sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Syech Zainal Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Varsha Salsabillah Wahyudi Wahyudi Warsih, Eko Winarsih Winarsih Windarsih, Yulia Zasmita, Eka Zulaikhah Dwi Jayanti