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Journal : Jagur: Jurnal Agroteknologi

Penampilan Morfologi dan Sitologi Tanaman Semangka (Citrullus lanatus Thunb.) Hasil Induksi Senyawa Kolkisin Aswat, Amarilla; Hayati, PK Dewi; Sutoyo; Warnita; Kuswandi
JURNAL AGROTEKNOLOGI UNIVERSITAS ANDALAS Vol 7 No 1 (2025)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jagur.7.1.11-17.2025

Abstract

Seedless watermelons, both seeds and fruits, possess a significantly higher economic value than their seeded counterparts. Serif Saga Agrihorti, a variety developed by the Tropical Fruit Research Institute, offers several advantages, including a sweet taste, high fruit yield, bright red flesh, and large fruit size. However, it is characterized by a relatively high number of seeds. The development of a new seedless variety from Serif Saga Agrihorti is crucial. Polyploid induction using mutagenic agents like colchicine is an effective method to obtain tetraploid female parents. This study aimed to identify the optimal method and colchicine concentration for inducing polyploidy in watermelons and to produce putative tetraploid plants. Polyploid induction was conducted using two methods: directly applying colchicine solution to plant shoots and soaking seeds in colchicine solution for 24 and 48 hours at concentrations of 0.2% and 0.4%. The observed traits included stem diameter, leaf length and width, stomatal length and width, pollen diameter, pollen viability, male and female flowering periods, harvesting time, fruit weight, fruit diameter, seed count, and seed dimensions (length and width). Data were analyzed using an unpaired t-test using the Statistical Tools for Agricultural Research (STAR) software. The results identified four putative tetraploid genotypes based on morphological observations of pollen diameter, stomatal size, and seed dimensions. In conclusion, seed soaking in colchicine solution effectively induces polyploidy in watermelons compared to shoot application.
Induki Mutasi dengan Sinar UV-C dan Seleksi In Vitro Kalus Kedelai yang Toleran Kekeringan Allatifa, Mumthaza; Gustian; Sutoyo
JURNAL AGROTEKNOLOGI UNIVERSITAS ANDALAS Vol 7 No 2 (2025)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jagur.7.2.121-126.2025

Abstract

Soybean (Glycine max. L) is a strategic commodity that plays a major role in providing nutritious food. One effort to increase soybean production is through the use of superior varieties. Dega-1 is a soybean variety that is very sensitive to drought so that it is necessary to develop soybeans cultivated in dry land which can be attempted through mutation breeding. The purpose of this study was to determine the duration of ultraviolet C light irradiation that can produce mutants and to obtain putative drought-tolerant mutants through ultraviolet C light mutation induction and in vitro selection (PEG 25%). This study was conducted from April to July 2024 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. This study was arranged in a Randomized Block Design with treatment duration of UV C light irradiation λ 254 nm with 10 treatment levels including no radiation, 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 105 minutes, 120 minutes, and 135 minutes. Each treatment consisted of 5 groups. The observation data were analyzed using simple statistical analysis using the mean and standard deviation calculations. The results showed that the highest percentage of putative drought-tolerant soybean mutants (PEG 6000 25%) was produced by 45 minutes of UV C irradiation, namely 3.33%. These mutants emerged from blackish-brown callus and developed into yellow callus and also regenerated into somatic embryos.  
Co-Authors Abdus Shomad Agustin Nurhayati Agustina Nurhayati Ahmad Zainuddin Allatifa, Mumthaza Ama Farida Sari Amaral, Carmelita Maria AMELIA SETYAWATI, AMELIA Arien Heryansyah Arif Yusron Afifi Aryani Sairun Aswat, Amarilla Bagus Saefulloh, Bagus Bambang Widiyatmoko Bayu Eko Broto Binti Mohd Mustamil , Norizah Budiman, Michael Jayanto Chairul Baria Chairul Bariah Chairul Bariah Chusnul Arif dedi irawan Dina Hendiani Dina Hendiyani Dwi Hanto Eko Daryanto Endang Syahriani Endriatmoko, Yosa Eny Wahyuning Purwanti Fajrinur Fajrinur Fatimah Zuhra Fausan, Ahmad Ferdiananda Chadafi, Muhammad Fuad Bahrul Ulum Galaciano, Antoninho Benjamin Marcos M.G. Do Rego Gustian Handoyo, GTPAK Hanita, Margaretha Harley Agustian Hayanuddin Safri Hayati, PK Dewi Hendiani, Dina Herlambang, Sri Mulyanto Husna, Syifa Ul Ibadil Maula, Farij Irianto Uripan, Koen Isguna, Teguh Kornelis, Veri Kuswandi Ludi Wishnu Wardana Lukman M Yusuf Maghriza, Zachri Mahfud M Gamar Muhammad Nur Ichwan Muhammad Reza Muhammad Saifrizal MULYADI Nafisah, A'isy Fiklik NASUTION, ATIKA AINI Nasya Nathalia Navrynda Putri Nelly Oxaria Silviana Devi Prastiyono, Suryo Prawira, Gilang Indracakti Purwanto, Moh Yanuar J Putra, Heriansyah Rahmad Khalis Rahmati Rahmati Rahmi Rahmi Rahmi Ramadhanis, Zainab Rantelino, Owen Riri Anggriani Rita Zahra Rosyadah, Rani Safri, Hayanuddin Saktioto Samad, Yusuf A Sandi, Ramona Sattya Arimurti Satyanto Krido Saptomo Sidik, Rizqi Fahma Soetikno, Lie Djaja Sofiyuddin, Hanhan A. Soleha, Siti Nur Son Ali Akbar Sri Murniyanti Sri Suryaningsum Sufritayati Suminar, Lintang Suri, Sulih Almaudi SUTOMO Syafiudin, Moh Fifik syahriani, endang Tri Putri Utami Tri Sudibyo Vicki Dwi Purnomo Warnita Widowati Yundrawan Ega Wibowo Yusuf A Samad Zuhri Nurisna Zulfikar Zuriani Ritonga