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Journal : ANNALES BOGORIENSES

Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece; D, Nurhayati; Farajallah, Ahmad; Anggraeni, A
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4236.897 KB) | DOI: 10.1234/59

Abstract

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene  on milk yield and percentage of milk protein  and  fat  in  local sheep. A total number of 83  heads of  lactating ewes  raised under an extensive management at  Jonggol Animal Science Teaching and Research Unit (JASTRU) of  the Faculty of Anima Science, Bogor Agricultural  University were  studied.  Research  activities  were  carried  out  through  some  step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P  R) then genotyped by single strand  conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on  the exon 7. The electrophoresis  pattern  revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for  those respective 5 type from the  highest to  the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%)  respectively. There were no  significant effect  polymorphism β-lactoglobulin gene on both milk yield and quality  in  I  cal  sheep at JASTRU.   Keywords : polymorphism. β-lactoglobulin in gene, PCR-SSCP and local sheep
Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Cece Sumantri; Nurhayati D; Ahmad Farajallah; A Anggraeni
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4236.897 KB) | DOI: 10.14203/ann.bogor.2008.v12.n1.17-24

Abstract

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene  on milk yield and percentage of milk protein  and  fat  in  local sheep. A total number of 83  heads of  lactating ewes  raised under an extensive management at  Jonggol Animal Science Teaching and Research Unit (JASTRU) of  the Faculty of Anima Science, Bogor Agricultural  University were  studied.  Research  activities  were  carried  out  through  some  step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P  R) then genotyped by single strand  conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on  the exon 7. The electrophoresis  pattern  revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for  those respective 5 type from the  highest to  the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%)  respectively. There were no  significant effect  polymorphism β-lactoglobulin gene on both milk yield and quality  in  I  cal  sheep at JASTRU.   Keywords : polymorphism. β-lactoglobulin in gene, PCR-SSCP and local sheep
Co-Authors A Anggraeni A Anggraeni A Anggraini A Batubara A. Anggraeni A. Anggraeni Achmad, Alfiyan Agus Alim Hakim AKICHIKA MIKAMI ALFIYAN, ACHMAD Ali Mashar Ali Suman Amir Hamidy Amir Hamidy AMIR HAMIDY Annawaty Annawaty, Annawaty Arma Aditya Kartika Aron Batubara Aron Batubara Arum Widayati, Kanthi Asri Febriana Asri Febriana, Asri B Tiesnamurti B. Tiesnamurti Baiq Farhatul Wahidah Bambang Purwantara Bambang Suryobroto Burhanuddin Burhanuddin C Sumantri C Sumantri C. Sumantri C. SUMANTRI Cece Sumantri D Perwitasari D, Nurhayati D. Herdiana D. Rahmat Daisy Wowor Daisy Wowor Daisy Wowor, Daisy Dede Setiadi Devi Sandriliana DEWI APRI ASTUTI DEWI PURNAMA Dwi Sendi Priyono Dyah Perwitasari DYAH PERWITASARI -FARAJALLAH E Andreas E. Andreas E. Andreas Eric N. Smith Eric N. Smith Erni Sulistiawati Eryk Andreas Farid Akhsani Fauzan, Muhammad Fakhri Ferdy Saputra H. Nuraini HANIM, NISFA HERA MAHESHWARI Hermansyah, Alfandy I Inounu Imas Batubara Irma Shita Arlyza Isdoni Bustaman J F Salamena Jarmuji Jarmuji Jeane Siswitasari Mulyana Joni Setiawan Jumadin, La Kaiin, Ekayanti Mulyawati KANTHI ARUM WIDAYATI Khairiza Lubis koekoeh santoso KREY KREY Kuswati Kuswati Lora purnamasari, Lora Luthfia Nuraini Rahman M Doloksaribu M Karmita M. Doloksaribu Mahendra, Arindina Maria Ulfah Maria Ulfah Mennofatria Boer Meutiya Agustina Meutiya Agustina, Meutiya Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Mufti Sudibyo N Satriani NEVIATY PUTRI ZAMANI Nirmala Fitria Firdhausi Nisfa Hanim Nisfa Hanim Novita Anggraeni Nurhayati D Nurlisa Alias Butet Nurulvadini Safrudin Paskah Partogi Agung Person Pesona Renta Pradana, Tengku Gilang Pudji Achmadi Putri, Vinna Windy R Diyono R Misrianti R R Noor R R Noor R.R. Noor R.R. Noor Rarah Ratih Adjie Maheswari RIKA RAFFIUDIN Rissa Herawati Ginting Riyanto, Awal Riyanto, Awal Rose Ida Ummah Rudi Alek Wahyudin Sasmita, Annissa Nuridfi Silvia Puspitasari Siti Lutvaniyah Slamet Mulyanto Smith, Eric N. Soemarno, Suprayogo Soemarno, Suprayogo Syahruddin Said Tanaka, Hiroyuki TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TARUNI SRI PRAWASTI Tatewaki, Takafumi Tri Eko Susilorini Tsuji, Yamato Vella Nurazizah Djalil Wahyudin Abd. Karim Widayat, Kanthi Arum i Widayati, Kanthi Wijayanti, Pristina Tuti Yanti Ariyanti Yanti Ariyanti Yusli Wardiatno