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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu dan Teknologi Kelautan Tropis Buletin Kimia VALENSI CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering BERITA BIOLOGI BIOMA Indonesian Journal of Chemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Indonesian Journal of Applied Research (IJAR) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Jamu

LAKSMI AMBARSARI

Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University Bogor 16680, Indonesia

Author-ID : 225737

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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KARAKTERISASI KITINASE ISOLAT BAKTERI RHIZOSFIR ASAL CIANJUR DAN AKTIVITASNYA TERHADAP PATOGEN Colletotrichum sp. Yadi Suryadi; Dwiningsih Susilowati; I Made Samudra; Mustika Permatasari; Laksmi Ambarsari
Bioma : Jurnal Ilmiah Biologi Vol. 9 No. 1: April 2020
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v9i1.6034

Kitinase mampu menghidrolisis kitin dan berpotensi sebagai agen biokontrol. Tujuan penelitian adalah memurnikan dan mengkarakterisasi kitinase dari isolat terpilih, serta menentukan kemampuan kitinolitiknya dalam penghambatan jamur Colletotrichum sp. Aktivitas kitinase ditentukan dengan metode Spindler, sementara kadar protein ditentukan dengan metode Bradford. Produksi kitinase isolat rizobakteri (isolat C5C) menunjukkan aktivitas enzim spesifik tertinggi sebesar 0.0489 U/mg melalui pemurnian parsial NH4SO4 70%, serta dapat meningkatkan kemurnian 13.97 kali dibandingkan ekstrak kasar. Karakterisasi kitinase isolat C5C menunjukkan bahwa enzim aktif optimal pada suhu 55°C, pH 7, waktu inkubasi 120 menit, serta memiliki nilai Km sebesar 1.300 x 103 mg/L dan Vmaks sebesar 0.0294 mgL-1detik-1. Aktivitas antifungi pada uji in vitro menunjukkan bahwa isolat C5C dapat menghambat pertumbuhan Colletotrichum sp.Kata kunci: biokontrol,Colletotrichum sp., kitinase, rizobakteri

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA Siti Warnasih; Diana Widiastuti; Uswatun Hasanah; Laksmi Ambarsari; Purwantiningsih Sugita
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

Evaluasi varietas, sumber eksplan dan strain Agrobacterium terhadap keberhasilan transformasi tebu dengan gen P5CS Evaluation of varieties, explant sources, and Agrobacterium strains for successful sugarcane transformation using P5CS gene Hayati MINARSIH; Dwi SUBIYARTI; Imron RIYADI; Soekarno Mismana PUTRA; Laksmi AMBARSARI
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract Genetic transformation can be used as an alter-native to develop sugarcane (Saccharum officinarum L.) tolerant to drought stress. P5CS gene has a role in biosynthesis of proline, an amino acid that accumulated under drought stress conditions. Transfer of a P5CS gene construct into plant cells in conjunction with regeneration of transgenic plantlets may develop sugarcane tolerant to drought stress. The aim of this research was to obtain an optimal transformation method which includes a suitable strain of Agrobacterium tumefaciens, and the best sugarcane explant and variety. The results showed that transfer of P5CS gene has been successfully carried out on sugarcane explants from solid media-derived calli, embryogenic calli and somatic embryos derived from temporary immersion system (TIS) culture. Whilst Agrobacterium strain LBA4404 was indicated as the most effective transformation vector. The regeneration of Kidang Kencana variety transformants from calli and somatic embryos was better than those of PS 881 and PS 891. The best performance of transformants based on the source of explants obtained from somatic embryos from TIS culture. Moreover, a succesfull Agrobacterium mediated transformation on sugarcane was indicated by transient expression of Gus gene and the ability of the transformants grew in a selection medium containing 50 ppm of kanamycin.Abstrak Transformasi genetik dapat digunakan sebagai upaya untuk merakit tebu (Saccharum officinarum L.) toleran terhadap cekaman kekeringan. Gen P5CS diketahui berperan dalam biosintesis prolin, yaitu asam amino yang umumnya terakumulasi ketika tanaman mengalami cekaman kekeringan. Transfor-masi gen P5CS dan regenerasi transgeniknya mungkin dapat menghasilkan tanaman tebu trans-genik yang toleran terhadap cekaman kekeringan. Tujuan penelitian ini adalah untuk mendapatkan metode transformasi yang optimum yang mencakup strain Agrobacterium tumefaciens yang sesuai, sumber eksplan dan varietas tebu terbaik sebagai target transformasi. Hasil penelitian menunjukkan bahwa transformasi gen P5CS telah berhasil dilakukan ke eksplan tebu baik yang berupa kalus asal media padat maupun kalus embriogenik dan embrio somatik asal kultur sistem perendaman sesaat (SPS). Sementara itu strain A. tumefaciens LBA4404 menunjukkan hasil yang paling efektif sebagai vektor transformasi. Pertumbuhan transforman baik pada kalus maupun embrio somatik pada varietas Kidang Kencana terlihat paling baik dibandingkan dengan varietas PS 881 dan PS 891. Sumber eksplan yang paling efektif adalah embrio somatik yang diperoleh dari kultur SPS. Keberhasilan transformasi tebu me-lalui Agrobacterium ditunjukkan oleh ekspresi transien dari gen GUS dan kemampuan dari trans-forman untuk tumbuh di media yang mengandung 50 ppm kanamisin.

Curcuminoids Content and α-Glucosidase Inhibition of ExtractPromising Lines of Curcuma xanthorrhiza RoxB. WARAS NURCHOLIS; LAKSMI AMBARSARI; GIA PERMASKU; LATIFAH k DARUSMAN; POPI ASRI KURNIATIN
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.086 KB)

Temulawak merupakan salah satu tanaman obat Indonesia yang termasuk dalam keluarga Zingiberaceae. Kurkuminoid merupakan salah satu senyawa bioaktif dalam temulawak yang dilaporkan memiliki aktivitas biologi. Pada penelitian ini dilakukan evaluasi kandungan kurkuminoid dan penghambatan enzim α-glukosidase dari ekstrak empat aksesi temulawak asal Jawa Barat (Sukabumi), Jawa Tengah (Wonogiri dan Karanganyar), Jawa Timur (Ngawi), dan satu ekstrak asal Bogor-Jawa Barat. Simplisia temulawak tersebutdiekstraksi secara bertahap dengan etanol dan n-heksan. Kandungan kurkuminoid diukur dengan menggunakan metode HPLC. Aktivitas penghambatan enzim α-glukosidase dilakukan secara in vitro dengan microplate reader pada 410 nm. Aksesi temulawak asal Wonogiri menunjukkan kandungan kurkuminoid tertinggi, dengan hasil pengukuran antara 35,57- 85,19 mg/g. Nilai IC50 untuk aktivitas penghambatan enzim α-glukosidase antara 333,27̵908,35 μg/mL, dengan IC50 terbaik adalah temulawak aksesi Wonogiri.Berdasarkan penelitian ini, aksesi temulawak asal Wonogiri sangat baik dalam hal kandungan kurkuminoid dan aktivitas penghambatan enzim α-glukosidase dibandingkan dengan aksesi asal Sukabumi, Karanganyar dan Ngawi, maupun dengan varietas asal Bogor.

Gelatin Extraction and Characterization from Femur Bones of Bovine and Porcine with Acid Process Purwantiningsih Sugita; Muhamad Rifai; Laksmi Ambarsari; Dyah Utami Cahyaning Rahayu; Hanhan Dianhar
Jurnal Jamu Indonesia Vol. 6 No. 1 (2021): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v6i1.188

Gelatin has been widely used as an additive in food industry pharmaceutical, and cosmetic. The similar physical appearance between bovine and porcine gelatin causes an issue for some communities like a Muslim due to awareness of halal food. This study aims to produce gelatin from femur bones of bovines with acid hydrolysis and their characteristics compared to standard gelatin of bovine and porcine. Bovine and porcine bones were soaked in 5% HCl for 10 days and every 2 days a HCl solution was replaced to get ossein. Ossein is hydrolyzed by gradual heating at 65, 75, and 85oC. Gelatin confirmed by the physico-chemical characters, FT-IR and analysis amino acid with HPLC.The results showed that the yield of bovine gelatin was 4.33%. The physico-chemical characters of bovine gelatin resulting from isolation and bovine gelatin standards are in conformity with porcine gelatin standards and meet the requirements of SNI 06-3735-1995 and GMIA. Therefore, bovine gelatin is specifically capable of substituting porcine gelatin for application in the pharmaceutical field. The FTIR spectrum of bovine gelatin shows the presence of amide A, amide I, amide II and amide III groups. The amino acid characters of gelatin were identified as glycine (13.57%) and proline (1.62%) for bovine gelatin and glycine (0.51%) and proline (0.09%) for porcine gelatin.

Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell Laksmi Ambarsari; Inda Setyawati; Rini Kurniasih; Popi Asri Kurniatin; Akhiruddin Maddu
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Glucose oxidase (GOx) is being developed for many applications such as an implantable fuel cell, due to its attractive property of operating under physiological conditions. This study reports the functional immobilization of glucose oxidase onto polyaniline-nanofiber-modified-carbon-paste-electrodes (GOx/MCPE) as bioanodes in fuel cell applications. In particular, GOx is immobilized onto the electrode surface via a linker molecule (glutaraldehyde). Polyaniline, synthesized by the interfacial polymerization method, produces a morphological form of nanofibers (100-120 nm) which have good conductivity. The performance of the polyaniline-modified-carbon-paste-electrode (MCPE) was better than the carbon- paste-electrode (CPE) alone. The optimal pH and temperature of the GOx/MCPE were 4.5 (in 100 mM acetate buffer) and 65 °C, respectively. The GOx/MCPE exhibit high catalytic performances (activation energy 16.4 kJ mol-1), have a high affinity for glucose (Km value 37.79 µM) and can have a maximum current (Imax) of 3.95 mA. The sensitivity of the bioelectrode also was high at 57.79 mA mM-1 cm-2.

DISSOLUTION BEHAVIOR, STABILITY AND ANTI-INFLAMMATORY ACTIVITY OF KETOPROFEN COATED TRIPOLYPHOSPHATE MODIFIED CHITOSAN NANOPARTICLE Jaya Hardi; Purwantiningsih Sugita; Laksmi Ambarsari
Indonesian Journal of Chemistry Vol 13, No 2 (2013)
Publisher : Universitas Gadjah Mada

Ketoprofen coated tripolyphosphate modified chitosan nanoparticle was resulted from ionic gelation process by ultrasonication, centrifugation, and spray dry methods. Particle was analyzed by particle Size Analyzer. Dissolution and stability of them have conducted by Row Hansen methods at 37±0.5 °C and 150 rpm and using climatic chamber at 40±2 °C and relative humidity 75±5% for 3 months, respectively. Anti-inflammatory activities have conducted by Plate Reader methods. The result showed that B formulae have particles diameter of 563.1±157.5 nm and entrapment efficiency of 89.663%, respectively. Dissolution behavior showed that ketoprofen restrained released within acid medium and maximum released within base medium of 11.35-15.87% and 45.73-99.25%, respectively. Kinetically, dissolution of ketoprofen within intestinal and gastric pH condition was Korsmeyers-Peppas kinetic models. While, stability behavior showed that B formula has ketoprofen percentage left in nanocapsule after 3 months greater than 90% with water content and shelf life of 19.68%, and 14.19 week, respectively, and degradation kinetic model followed Avrami-Eroveef equation. Anti-inflammation test showed that B formula had the COX-2 inhibitory activity of 90.86% is higher than free ketoprofen.

Homology Modeling and Structural Dynamics of the Glucose Oxidase Farhan Azhwin Maulana; Laksmi Ambarsari; Setyanto Tri Wahyudi
Indonesian Journal of Chemistry Vol 20, No 1 (2020)
Publisher : Universitas Gadjah Mada

Glucose oxidase from Aspergillus niger IPBCC.08.610 (GOD_IPBCC) is a locally sourced flavoenzyme from Indonesia that can potentially be developed in a variety of industrial processes. Although this enzyme has a high activity in catalyzing the redox reactions, the use of this enzyme was still limited to be applied as glucose biosensor. Using information from the amino acid sequences, a computational structure of GOD_IPBCC was therefore designed by homology modeling method using two homologous structures of GOD from protein data bank (1CF3 and 5NIT) as the templates. The quality of the resulting structures was evaluated geometrically for selection of the best model, and subsequently, 50 ns of MD simulations were carried out for the selected model as well as the corresponding template. Results obtained from the validation analysis showed that the 1CF3 template-built structure was selected as the best reliable model. The structural comparison exhibited that the best-modeled structure consisted of two functional domains and three catalytic residues similarly to the corresponding experimental structure. The overall dynamic behavior of the 50 ns of the structure was structurally stable and comparable with that of the positive control both from globally and locally observations. Implications of these stable nature within the best-modeled structure unfold the possibilities in search of notable residues and their roles to enhance enzyme thermostability.

Molecular Docking Study of IPBCC.08.610 Glucose Oxidase Mutant for Increasing Gluconic Acid Production Shobiroh Nuur' Alimah; Tony Ibnu Sumaryada; Waras Nurcholis; Laksmi Ambarsari
Jurnal Kimia Sains dan Aplikasi Vol 25, No 5 (2022): Volume 25 Issue 5 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Glucose oxidase (GOD) is an oxidoreductase enzyme that catalyzes the oxidation of glucose to gluconolactone and hydrogen peroxide. Then, gluconolactone will be hydrolyzed to gluconic acid. The wide application of gluconic acid in various industries has increased production demand. However, glucose concentrations higher than 40% (w/w) inhibited the conversion of glucose to gluconic acid due to a decrease in the oxygen solubility concentration at pH 6, 30℃, and 1 bar pressure. Therefore, decreasing the value of Km is predicted to reduce saturation and enhance gluconic acid production. This study aimed to analyze the interaction between the IPBCC.08.610 GOD mutant with β-D-Glucose in improving gluconic acid production by decreasing the Km value. Mutations were performed in silico using Chimera and then docked using AutoDock Vina. The mutations resulted in distinct ligand poses in the binding pocket, different -OH conformations of the ligands, and changes in the T554M/D578P mutant’s hydrophobicity index (554 mutated from threonine to methionine, and 578 mutated from aspartate to proline), and decreased ΔG and Km values in the H559D mutant (559 mutated from histidine to aspartate), D578P and T554M/D578P. This decrease might strengthen the ligand-receptor interaction, increasing gluconic acid production. The H559D was the best mutant to increase production based on the ΔG, Km value, and stability due to the addition of hydrogen bonds.

Produk Reaksi Maillard (MRP) sebagai Anti Bakteri dan Pengendali Kadar Dekstran dalam Nira Agustina L. N. Aminin; Laksmi Ambarsari; H. M. Mochtar
Jurnal Kimia Sains dan Aplikasi Vol 5, No 2 (2002): Volume 5 Issue 2 Year 2002
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

MRP adalah hasil reaksi pencoklatan non enzimatik yang dapat dibuat dengan merefluks larutan yang mengandung asam amino dan gula reduksi. Aktivitas MRP diamati dengan meneliti pertumbuhan mikroorganisme dari nira yang ditumbuhkan dalam medium sintetik melalui pengukuran turbiditas. Pengaruh MRP terhadap produksi dekstran diamati melalui analisa kadar dekstran menggunakan metode Haze. Hasil pengamatan baik terhadap pertumbuhan mikroba maupun kadar dekstran menunjukkan bahwa MRP yang dibuat dari lisin dan glukosa dengan waktu pemanasan lima jam dan menggunakan gas nitrogen memberikan penghambatan sampai 50%.

Co-Authors -, Suryani . SURYANI A.E. Zainal Hasan Ade Heri Mulyati Adrian Adiva Aghnia, Diya Agnia Nurul Jannati Agung Isnanto Agustina L.N. Aminin Ahmad Irvan Pratama Ainia Hanifitri Aisyah Girindra Akbar, Nadhira Fathiaz Akhiruddin Maddu Akhmad Endang Zainal Hasan Alimah, Shobiroh Nuur Ambarsavitri, Rahmadhani ANINDA INDRIANI Anja Meryandini Annisa , Alifia Mutiara Annisa Dhiya Athiyyah Khanza Aqilah, Rifany Fairuz Aris Tri Wahyudi Arwansyah Arwansyah Aryani, Hanifah Asrul Muhamad Fuad, Asrul Muhamad Assifah Eryandini Asvarhoza, Genta AYU LESTARI Azhari, Muhammad Alwin Chelsea Chelsea, Chelsea Chelsy Narita Choirunnisa Miftahul Jannah Deki Geraldi Dewi Seswita Zilda Diana Widiastuti DIMAS ANDRIANTO Djarot Sasongko Hami Seno DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Dwi SUBIYARTI Dwicesaria, Maheswari Alfira Dwiningsih Susilowati Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Efi Sanfitri Harahap Elvaza, Azzara Putri Faliha Arinda Lestari Farhan Azhwin Maulana Fathin, Muhammad Faris Fatriani, Rizka Febrianti, Riska Febriyanto, Dimas G Jeni christi A GIA PERMASKU Giovanny, Lisa Gustini Syahbirin H. M. Mochtar Hamidah, Wanda Hanhan Dianhar Harahap, Efi Sanfitri HARTUTIK EKA SUSANTI Hasim - Hasim Hasim Hayati Minarsih Heri Purwoto Hidayat, Radika Evita Hudayanti, Martini Husnawati Husnawati Husnawati, . I MADE ARTIKA I Made Samudera I Made Samudera, I Made I MADE SAMUDRA Ifa Manzila Ifa Manzila Ika Yuni Astutik Ika Yuni Astutik, Ika Yuni Ike Wahyuni Putri Ilham Kurniawan Ilham Kurniawan Imelia Dewi Imron RIYADI Inayah, Mazidah Noer Inda Setyawati, Inda Irma Rahmayani Isnanto, Agung Jaya Hardi Kandi, Rizky Putra Kapitan, Origenes Boy Karichsa Hariana Komalasari, Nurma Angeliani Latifah K Darusman Lestari, Faliha Arinda Liliyani , Ni Putu Peggy Lisa Giovanny Made Suhandana Madyastuti, Rini Marfira, Nurul Mega Safithri Miantika, Shafillah Muhamad Rifai Muhamad Rifai Muhammad Alwin Azhari Muhammad Farhan Muhammad Faris Fathin Muhammad Halim, Muhammad Mustika Luthfia Mustika Permatasari Mustopa, Syahrul Nabila Nur Nafiati Natalia Marbun, Natalia NISA RACHMANIA MUBARIK Nur Qadri Rasyid Nurhidayat Nurhidayat Nurul Marfira Nuur'Alimah, Shobiroh Nuur’Alimah, Shobiroh Pratama, Ahmad Irvan Pratiwi, Anggita Aziz Purwantiningsih Sugita Purwanto, Ukhradiya Magharaniq Safira Purwatiningsih Sugita Puspa Julistia Puspita Putra, Adriansyah Nanda Putri, Ike Wahyuni Radika Evita Hidayah Rahayu, Dyah Utami Cahyaning Rahman, Syabilla Aulia Rasyid, Nur Qadri Ridho Pratama Rifany Fairuz Aqilah Riki Riki Riksa Nur Wahyuni Rini Kurniasih, Rini Rini Novita Riska Febrianti Rosyidah, Rara Annisaur Rury Eryna Putri Sanro Tachibana Sarmila, Sarmila Setyanto Tri Wahyudi Shobiroh Nuur' Alimah Shobiroh Nuur'Alimah Siti Nur'aeni Siti Nurjanah Siti Warnasih Slameut, Fitria Soekarno Mismana Putra Steffanus Gozales Suryani - Suryani Suryani Syamsul Falah TATI NURHAYATI Tirta Setiawan Tony Sumaryada Tri Puji Priyatno Tri Puji Priyatno Uswatun Hasanah Vino , Okta Wahyuni, Riksa Nur Waras Nurcholis Winarti, Novia Yadi Suryadi Yadi Suryadi Yudha, Trah Zahra Silmi Muscifa Zuniar Subastian

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