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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu dan Teknologi Kelautan Tropis Buletin Kimia VALENSI CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering BERITA BIOLOGI BIOMA Indonesian Journal of Chemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Jurnal Agronomi Tanaman Tropika (JUATIKA) Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Indonesian Journal of Applied Research (IJAR) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Jamu Biodiversitas Journal of Biological Diversity
LAKSMI AMBARSARI
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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The Potential of Murraya koenigii as a PfAp4AH Inhibitor for Malaria Drug Development Inda Setyawati; Assifah Eryandini; Nadine Rudiyana; Laksmi Ambarsari; I. Made Artika
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 2 (2025): Vol. 7 No. 2 Mei (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i2.4585

Abstract

Murraya koenigii, a medicinal plant from the Rutaceae family, is traditionally used as a flavoring agent and is known for its bioactive carbazole alkaloids. Native to the Indo-Malaysian region, China, Sri Lanka, and New Caledonia, this plant has shown potential for therapeutic applications, including antimalarial activity. Malaria, particularly caused by Plasmodium falciparum, remains a significant global health challenge due to rising drug resistance. PfAp4AH, an enzyme involved in diadenosine tetraphosphate (Ap4A) metabolism, has emerged as a promising target for novel antimalarial drugs. This study employed computational approaches—binding site prediction and virtual screening through molecular docking—to identify potential PfAp4AH inhibitors from 156 carbazole alkaloids derived from M. koenigii. Ten compounds demonstrated stronger binding affinities than ATP, with compound 1 showing the highest inhibitory potential through strong and diverse interactions with key residues Tyr87, His43, Pro133, and Leu136. These findings underscore the importance of specific ligand–residue interactions in determining binding strength. The identified compounds, especially compound 1, present promising leads for further experimental validation. While initial bioactivity and toxicity profiles are favorable, comprehensive bioavailability and toxicological evaluations are needed to advance these compounds as antimalarial drug candidates.
Application of Maggot-based Chitosan for Edible Coating on Cut Papaya (Carica papaya L.) Sri Wahyuni; Hanaa Ashiilah; Laksmi Ambarsari; Haryo Tejo Prakoso; Siswanto Siswanto
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 2 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.2.193

Abstract

Minimally processed papaya fruits are susceptible to postharvest losses due to Colletotrichum sp. infection during storage, necessitating additional postharvest treatments such as putting an edible coating to the surface to minimize microbial growth and weight loss. Exuviae from Hermetia illucens maggots, which are rarely utilized, contain chitin, which can be converted into chitosan. It also possesses antibacterial properties, making it a viable candidate for edible coatings on papaya fruits. There are various approaches for using edible coating processes. Two of these are spray and immersion methods. In this study, the antifungal activity of synthesized chitosan was tested against Colletotrichum sp. and applied as an edible coating to sliced papaya fruit via spray and immersion ways. The effectiveness of the two strategies was compared. The findings revealed that black soldier fly-derived chitosan at varied concentrations (25 to 100 ppm) had antifungal action against the tested fungus. and can be used as an edible coating on sliced papaya fruit. When applied by spray and stored at 10°C, chitosan coating at a concentration of 100 ppm is highly effective in inhibiting Colletotrichum sp. growth and fruit weight loss, extending its shelf life by more than 20 days. Keywords: chitosan, Colletotrichum sp., cut papaya, edible coating
Inovasi Pangan Berbasis Tempe sebagai Upaya Pemberdayaan Masyarakat untuk Tumbuh Kembang Anak Menuju Indonesia Emas 2045 Rini Kurniasih; Mega Safithri; Laksmi Ambarsari; Popi Asri Kurniatin; Ukhradiya Magharaniq Safira Purwanto; Dimas Andrianto; Syamsul Falah; Rara Annisaur Rosyidah; Maheswari Alfira Dwicesaria; Martini Hudayanti; Ayu Lestari; Syabilla Aulia Rahman; Anggita Aziz Pratiwi; Rahmadhani Ambarsavitri
Qardhul Hasan: Media Pengabdian kepada Masyarakat Vol. 12 No. 1 (2026): Qardhul Hasan: Media Pengabdian kepada Masyarakat
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/qh.v12i1.22074

Abstract

Indonesia menghadapi tantangan besar dalam mewujudkan generasi yang sehat, cerdas, dan produktif menuju Indonesia Emas 2045, dengan prevalensi stunting nasional yang masih mencapai 19,8% pada tahun 2024. Peningkatan gizi sejak dini melalui pangan lokal bergizi tinggi seperti tempe, sebagai sumber protein nabati, merupakan solusi yang efektif. Program pemberdayaan masyarakat di Desa Cipalaha, Garut, melibatkan ibu dengan anak usia 0–5 tahun, ibu hamil, serta kader Posyandu dan PKK. Kegiatan meliputi penilaian status gizi anak, edukasi gizi dan stunting, pelatihan pembuatan nugget tempe dan sari kedelai, serta evaluasi melalui kuesioner orang tua, wawancara, dan uji organoleptik. Hasil menunjukkan 24% balita tergolong pendek atau sangat pendek, dan seluruh anak di atas lima tahun memiliki berat badan kurang, menandakan kekurangan energi kronis. Tingkat pendidikan, pekerjaan, dan pendapatan orang tua berpengaruh kuat terhadap risiko stunting. Program ini berhasil meningkatkan pengetahuan, keterampilan, dan motivasi peserta dalam memproduksi serta mengonsumsi olahan tempe bergizi tinggi dengan tingkat penerimaan yang baik. Temuan ini menegaskan pentingnya inovasi pangan lokal dan pemberdayaan masyarakat dalam mendukung pertumbuhan anak dan tujuan Indonesia Emas 2045.
Molecular Docking of Active Compound of Okra (Abelmoschus esculentus L.) on α-Glucosidase as Antidibetic Mellitus Drug Candidate Shobiroh Nuur'Alimah; Laksmi Ambarsari; Muhammad Farhan
Jurnal Jamu Indonesia Vol. 11 No. 1 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i1.360

Abstract

Diabetes mellitus (DM) is a leading cause of death among degenerative diseases, primarily due to impaired insulin function that disrupts carbohydrate metabolism. One therapeutic strategy involves α-glucosidase inhibitors that delay glucose absorption. Abelmoschus esculentus (okra) is rich in phenolic and flavonoid compounds with antihyperglycemic and antioxidant activity, suggesting potential as α-glucosidase inhibitors. This study evaluated the inhibitory potential of okra-derived compounds against α-glucosidase using in silico molecular docking with PLANTS and YASARA Structure. The analysis included physicochemical screening, ligand–receptor preparation, and docking simulations, assessing docking score, Gibbs free energy (ΔG), and dissociation constant (Kd). Cannabiscitrin, a flavonoid from okra, demonstrated the strongest binding affinity, outperforming the reference drug acarbose in all parameters. These findings suggest its potential as an alternative antidiabetic agent. 
Metabolite Profiling and Bioactivity Assessment of Antioxidant and Antibacterial Properties in Bulb Fractions of Eleutherine bulbosa (Mill.) Urb Nurma Angeliani Komalasari; Natalia Natalia Marbun; Gustini Syahbirin; Laksmi Ambarsari; Purwatiningsih Sugita
Jurnal Jamu Indonesia Vol. 11 No. 1 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i1.388

Abstract

Eleutherine bulbosa is known to contain diverse secondary metabolites with pharmacological potential. This study aimed to identify the bioactive compounds in the ethanol, n-hexane, and unsaponified n-hexane fractions of E. bulbosa bulbs and evaluate their antioxidant and antibacterial activities. The bulbs were extracted using ethanol, followed by n-hexane fractionation and saponification. Antioxidant activity was determined using the DPPH assay, and antibacterial activity was assessed against Staphylococcus aureus and Escherichia coli using the disc diffusion method. LC-MS/MS and GC-MS analyses identified compounds such as eleutherol, resveratrol, coniferaldehyde, and stigmasterol. The ethanol fraction exhibited the highest antioxidant activity (IC₅₀ = 47.54 ± 0.03 mg/L), while the unsaponified n-hexane fraction showed the strongest antibacterial effect (inhibition zone = 16.12 ± 0.16 mm). These findings confirm the potential of E. bulbosaas a natural source of antioxidant and antibacterial agents.
Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization Rury Eryna Putri; Nisa Rachmania Mubarik; Laksmi Ambarsari; Aris Tri Wahyudi
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220956

Abstract

Abstract. Putri RE, Mubarik NR, Ambarsari L, Wahyudi AT. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas 22: 4067-4077. Biocontrol of Fusarium oxysporum, a phytopathogenic fungus that causes plant wilt can be approached with cell-wall degrading enzymes such as ?-glucanase. The aim of this study was to evaluate the prospective ability in glucanase production from several soil bacterial isolates and to characterize its ?-glucanase activity of ammonium sulfate precipitation, and to determine its antifungal activity against F. oxysporum in vitro. Twenty bacterial isolates were screened qualitatively and quantitatively as ?-glucanase producers. The results showed that the prospective isolate W3.15 can produce ?-glucanase on glucan agar as the selection medium. From 16S rRNA sequences identification, the isolate belongs to the genus Bacillus, closely related to Bacillus subtilis. The enzyme activity of the ammonium sulfate fraction of isolate W3.15 is optimum at a pH of 7 and temperature range of 60-80oC. B. subtilis W3.15 exhibits high inhibition against the mycelial growth of F. oxysporum and significantly reduced fungal biomass.
Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia SURYANI SURYANI; ASMAUL CHUSNA AL ANSHORY; MARLIN MARLIN; I MADE ARTIKA; LAKSMI AMBARSARI; Waras Nurcholis
Biodiversitas Journal of Biological Diversity Vol. 23 No. 4 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230434

Abstract

Abstract. Suryani, Al Anshory AC, Marlin, Artika IM, Ambarsari L, Nurcholis W. 2022. Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia. Biodiversitas 23: 1998-2003. Curcuma zanthorrhiza Roxb. and C. aeruginosa Roxb. are medicinal plants belonging to the Zingiberaceae family, which contained phenolic compounds. Phenolic is one of the antioxidant compounds. This study aimed to investigate variation on the total phenolic and antioxidant activity of three C. zanthorrhiza varieties and twenty C. aeruginosa genotypes cultivated in three different locations in West Java, Indonesia. Total phenolic content was determined using the Folin-Ciocalteu reagent, whereas antioxidant activity was used spectrophotometry using DPPH method. The total phenolic content ranged from 2.403 to 7.539 mg GAE/ g DW) differed significantly among the C. zanthorrhiza and C. aeruginosa varieties/genotypes, with the highest concentration found in the Cursina 3 variety of C. zanthorrhiza in Cianjur. The antioxidant activity ranged from 2.443 to 14.960 µmol TE/g DW, with the maximum activity identified in the Cursina 2 variety of C. zanthorrhiza in the Bogor location. G1, G6, G13, and G16 were identified as stable genotypes for phenolic antioxidant production. Three C. zanthorrhiza cultivars demonstrated higher total phenolic content and antioxidant activity than all C. aeruginosa genotypes in all planting areas. C. zanthorrhiza cultivars are the most promising source of phenolic content and antioxidant activity and are thus recommended for mass plantation at suitable locations in West Java, Indonesia to maximize potential.
Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes RAISADIBA PUTRI BOVANI; NOVIAN LIWANDA; IRMANIDA BATUBARA; LAKSMI AMBARSARI; WARAS NURCHOLIS
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250236

Abstract

Abstract. Bovani RP, Liwanda N, Batubara I, Ambarsari L, Nurcholis W. 2024. Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes. Biodiversitas 25: 763-769. Orthosiphon aristatus (Blume) Miq., also known as kumis kucing in Indonesia, is a medicinal plant belonging to the Lamiaceae family that contains various phytochemical compounds with antioxidant properties. This study investigated the phenolic content, flavonoid, rosmarinic acid,  sinensetin,  and antioxidant capacity of 15 genotypes of O. aristatus ethyl acetate extracts. Therefore, 15 genotypes were analyzed using quercetin as the standard for Total Phenolic Content (TPC) using a colorimetric method with gallic acid and Total Flavonoid Content (TFC). Sinensentin and rosmarinic acid were measured by High-Performance Liquid Chromatography (HPLC). Antioxidant capacity was determined using Trolox as a standard antioxidant by 2-Diphenyl Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays. The results revealed a diverse range of phytochemical compounds, with total phenolic content ranging from 3.39 to 13.80 mg GAE/g DW, total flavonoid content from 0.20 to 1.43 mg QE/g DW, rosmarinic acid from 0.02 to 0.19 mg/g DW, and sinensetin from 1.61 to 21.67 mg/g DW. Furthermore, the antioxidant assays demonstrated varying degrees of efficacy, with DPPH ranging from 2.09 to 13.98 ?mol TE/g DW and FRAP from 0.07 to 2.96 ?mol TE/g DW. Genotypes A10, A11, and A12 exhibited superior phytochemical contents and antioxidant capacities, highlighting their potential as valuable sources of medicinal compounds.
Purification and Molecular Characterization of Protease from Lacticaseibacillus paracasei IN17 Isolated from Fermented Fish (Inasua) Sakina Maya Maulawi; Nisa Mubarik; Laksmi Ambarsari
Biosaintifika: Journal of Biology & Biology Education Vol. 18 No. 1 (2026): April 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v18i1.39679

Abstract

Protease is a protein degrading enzyme that is widely used in the food fermentation and protein processing industries. An example of a fermented fish product known as inasua, which originates from Maluku in Indonesia, is a proteolytic bacterial source. The aims of this study were to purify, characterize, and analyze the molecular characteristics of the protease produced by Lacticaseibacillus paracasei IN17. Proteases from extracellular and intracellular fractions were partially purified using dialysis and ammonium sulfate precipitation. Metal ion effects, kinetic parameters, electrophoretic analysis, and the ideal pH and temperature were all used to assess the enzymatic properties. The extracellular protease enzyme showed an increase in specific activity from 0.079 to 0.182 U mg-¹ with a yield of 18.9%. Maximum activity was observed at pH 6.0 for the extracellular enzyme and pH 7.0 for the intracellular fraction at 37 °C. Mn²⁺ enhanced the activity, and the kinetics analysis showed higher catalytic efficiency in the extracellular enzyme. An active protein band ~26 kDa was detected, and structural modeling revealed a conserved Ser–His–Asp catalytic triad. This work seeks to expand current knowledge of LAB proteolytic systems derived from non-dairy fermented foods by integrating purification strategies, enzymatic profiling, and gene analysis. This research supports SDGs 3 and SDGs 12 by promoting functional food development and responsible utilization of local microbial resources.
Co-Authors -, Suryani . SURYANI A.E. Zainal Hasan Ade Heri Mulyati Adrian Adiva Aghnia, Diya Agnia Nurul Jannati Agung Isnanto Agustina L.N. Aminin Ahmad Irvan Pratama Ainia Hanifitri Aisyah Girindra Akbar, Nadhira Fathiaz Akhiruddin Maddu Akhmad Endang Zainal Hasan Alimah, Shobiroh Nuur Andiany, Fairuz Eka Anggita Aziz Pratiwi ANINDA INDRIANI Anja Meryandini Annisa , Alifia Mutiara Annisa Dhiya Athiyyah Khanza Aris Tri Wahyudi Aris Tri Wahyudi Arwansyah Arwansyah Aryani, Hanifah ASMAUL CHUSNA AL ANSHORY Asrul Muhamad Fuad, Asrul Muhamad Assifah Eryandini Assifah Eryandini Asvarhoza, Genta Ayu Lestari Azhari, Muhammad Alwin Chelsea Chelsea, Chelsea Chelsy Narita Choirunnisa Miftahul Jannah Deki Geraldi Dewi Seswita Zilda Diana Widiastuti DIMAS ANDRIANTO Dimas Andrianto Djarot Sasongko Hami Seno DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Dwi SUBIYARTI Dwiningsih Susilowati Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Efi Sanfitri Harahap Elvaza, Azzara Putri Faliha Arinda Lestari Farhan Azhwin Maulana Fathin, Muhammad Faris Fatriani, Rizka Febrianti, Riska Febriyanto, Dimas G Jeni christi A GIA PERMASKU Giovanny, Lisa Gustini Syahbirin Gustini Syahbirin H. M. Mochtar Hamidah, Wanda Hanaa Ashiilah Hanhan Dianhar Harahap, Efi Sanfitri HARTUTIK EKA SUSANTI Haryo Tejo Prakoso Hasim - Hasim Hasim Hayati Minarsih Heri Purwoto Hidayat, Radika Evita Husnawati Husnawati Husnawati, . I MADE ARTIKA I Made Samudera I Made Samudera, I Made I MADE SAMUDRA Ifa Manzila Ifa Manzila Ika Yuni Astutik Ika Yuni Astutik, Ika Yuni Ike Wahyuni Putri Ilham Kurniawan Ilham Kurniawan Imelia Dewi Imron RIYADI Inayah, Mazidah Noer Inda Setyawati Inda Setyawati, Inda Irma Rahmayani IRMANIDA BATUBARA Isnanto, Agung Jaya Hardi Kandi, Rizky Putra Kapitan, Origenes Boy Karichsa Hariana Latifah K Darusman Lestari, Faliha Arinda Liliyani , Ni Putu Peggy Lisa Giovanny Made Suhandana Madyastuti, Rini Maheswari Alfira Dwicesaria Marfira, Nurul MARLIN MARLIN Martini Hudayanti Mega Safithri Mega Safithri Miantika, Shafillah Muhamad Rifai Muhamad Rifai Muhammad Alwin Azhari Muhammad Farhan Muhammad Faris Fathin Muhammad Halim, Muhammad Mustika Luthfia Mustika Permatasari Mustopa, Syahrul Nabila Nur Nafiati Nadine Rudiyana Natalia Natalia Marbun Nisa Mubarik Nisa Rachmania Mubarik NISA RACHMANIA MUBARIK NOVIAN LIWANDA Nur Qadri Rasyid Nurhidayat Nurhidayat Nurma Angeliani Komalasari Nurul Marfira Nuur’Alimah, Shobiroh Popi Asri Kurniatin Pratama, Ahmad Irvan Purwantiningsih Sugita Purwanto, Ukhradiya Magharaniq Safira Purwatiningsih Sugita Puspa Julistia Puspita Putra, Adriansyah Nanda Putri, Ike Wahyuni Radika Evita Hidayah Rahayu, Dyah Utami Cahyaning Rahmadhani Ambarsavitri RAISADIBA PUTRI BOVANI Rara Annisaur Rosyidah Rasyid, Nur Qadri Ridho Pratama Rifany Fairuz Aqilah Riki Riki Riksa Nur Wahyuni Rini Kurniasih Rini Kurniasih, Rini Rini Novita Riska Febrianti Rury Eryna Putri Rury Eryna Putri Sakina Maya Maulawi Sanro Tachibana Sarmila, Sarmila Setyanto Tri Wahyudi Shobiroh Nuur' Alimah Shobiroh Nuur'Alimah Shobiroh Nuur'Alimah Siswanto Siswanto Siti Nur'aeni Siti Nurjanah Siti Warnasih Slameut, Fitria Soekarno Mismana Putra Sri Wahyuni Steffanus Gozales Suryani - SURYANI SURYANI Suryani Suryani Syabilla Aulia Rahman Syamsul Falah Syamsul Falah TATI NURHAYATI Tirta Setiawan Tony Sumaryada Tri Puji Priyatno Tri Puji Priyatno Uswatun Hasanah Vino , Okta Wahyuni, Riksa Nur WARAS NURCHOLIS Waras Nurcholis Waras Nurcholis Winarti, Novia Yadi Suryadi Yadi Suryadi Yudha, Trah Zahra Silmi Muscifa Zuniar Subastian