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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu dan Teknologi Kelautan Tropis Buletin Kimia VALENSI CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering BERITA BIOLOGI BIOMA Indonesian Journal of Chemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Indonesian Journal of Applied Research (IJAR) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Jamu
LAKSMI AMBARSARI
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Metabolite Profiling and Bioactivity Assessment of Antioxidant and Antibacterial Properties in Bulb Fractions of Eleutherine bulbosa (Mill.) Urb Komalasari, Nurma Angeliani; Natalia Marbun, Natalia; Syahbirin, Gustini; Ambarsari, Laksmi; Sugita, Purwatiningsih
Jurnal Jamu Indonesia Vol. 11 No. 1 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i1.388

Abstract

Eleutherine bulbosa is known to contain diverse secondary metabolites with pharmacological potential. This study aimed to identify the bioactive compounds in the ethanol, n-hexane, and unsaponified n-hexane fractions of E. bulbosa bulbs and evaluate their antioxidant and antibacterial activities. The bulbs were extracted using ethanol, followed by n-hexane fractionation and saponification. Antioxidant activity was determined using the DPPH assay, and antibacterial activity was assessed against Staphylococcus aureus and Escherichia coli using the disc diffusion method. LC-MS/MS and GC-MS analyses identified compounds such as eleutherol, resveratrol, coniferaldehyde, and stigmasterol. The ethanol fraction exhibited the highest antioxidant activity (IC₅₀ = 47.54 ± 0.03 mg/L), while the unsaponified n-hexane fraction showed the strongest antibacterial effect (inhibition zone = 16.12 ± 0.16 mm). These findings confirm the potential of E. bulbosaas a natural source of antioxidant and antibacterial agents.
Optimization of Extraction Conditions to Enhance Polyphenol Content and Antioxidant Activity İn Orthosiphon Aristatus Leaves Febriyanto, Dimas; Setyawati, Inda; Ambarsari, Laksmi; Nurcholis, Waras
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.16820

Abstract

ABSTRACT. Orthosiphon aristatus (Java tea) is a medicinal plant known for its high polyphenol content and antioxidant properties. Unlike previous studies that focused solely on solvent type or temperature, this study simultaneously optimizes temperature and pH extraction parameters to achieve maximal bioactive compound recovery. This study aimed to optimize extraction parameters—temperature and pH—to enhance polyphenol yield and antioxidant activity in methanolic extracts of Java tea leaves. Samples were extracted at temperatures ranging from 30 °C to 80 °C (pH 7) and pH levels from 2 to 6 (at room temperature). Total phenolic content (TPC) and total flavonoid content (TFC) were determined to quantify polyphenol levels, and antioxidant activity was evaluated using four complementary in vitro assays: DPPH, ABTS, FRAP, and CUPRAC. The highest TPC (9.68 mg GAE/g DW) and TFC (6.53 mg QE/g DW) were observed at 70 °C and 80 °C, respectively. Maximum antioxidant activities were observed at 70–80 °C and pH 2–3, with peak values of 102.71 µmol TE/g DW (ABTS), 101.9 µmol TE/g DW (FRAP), and 56.12 µmol TE/g DW (CUPRAC). A strong correlation was found between phenolic content and antioxidant capacity. These findings highlight the critical role of extraction conditions in maximizing the biofunctional potential of O. aristatus and support its application in pharmaceutical and nutraceutical formulations. The study provides a scientific basis for developing standardized extraction protocols to improve functional ingredient consistency in herbal product development. Keywords: Antioxidant activity, extraction optimization, Orthosiphon aristatus, pH effect, temperature
Demonstrasi Pengolahan Bubuk Jahe Merah sebagai Ide Wirausaha Minuman Instan Berkhasiat di Desa Batu Busuk Asvarhoza, Genta; Halim, Muhammad; Sarmila, Sarmila; Putra, Adriansyah Nanda; Annisa , Alifia Mutiara; Winarti, Novia; Hamidah, Wanda; Vino , Okta; Yudha, Trah; Elvaza, Azzara Putri; Ambarsari, Laksmi
Jurnal Pusat Inovasi Masyarakat Vol. 5 No. 1 (2023): April 2023
Publisher : Direktorat Pengembangan Masyarakat Agromaritim, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpim.5.1.68-76

Abstract

Umumnya jahe digunakan oleh masyarakat di Desa Batu Busuk untuk berbagai kepentingan, misalnya sebagai campuran bahan makanan dan minuman. Jahe merah memiliki banyak manfaat yang berguna untuk kesehatan. Selama masa pandemi ini kebutuhan masyarakat akan jahe mengalami peningkatan sehingga perlu adanya inovasi produk olahan jahe salah satunya bubuk jahe merah instan. Pengolahan jahe merah yang dipadukan dengan gula serta bisa ditambahkan rempah lain sebagai pelengkap sehingga menjadi bubuk jahe merah yang dinamakan jahe next level (JNL). Olahan jahe merah ini nantinya bisa dijadikan minuman yang berkhasiat untuk kesehatan. Pengolahan jahe merah menjadi bubuk jahe merah meningkatkan nilai mutu produk pangan tersebut di samping khasiatnya yang sangat bermanfaat. Bubuk jahe merah dapat menjadi produk pangan alternatif bagi masyarakat yang ingin mengonsumsi minuman jahe dengan penyajian yang mudah yaitu dengan cara diseduh dengan air hangat. Selain itu, pengolahan bubuk jahe merah juga dapat menjadi ladang wirausaha yang menguntungkan bagi masyarakat di Desa Batu Busuk. Melalui demonstrasi pengolahan bubuk jahe merah diharapkan dapat memberikan ide produk wirausaha minuman berkhasiat bagi masyarakat di Desa Batu Busuk.
Co-Authors -, Suryani . SURYANI A.E. Zainal Hasan Ade Heri Mulyati Adrian Adiva Aghnia, Diya Agnia Nurul Jannati Agung Isnanto Agustina L.N. Aminin Ahmad Irvan Pratama Ainia Hanifitri Aisyah Girindra Akbar, Nadhira Fathiaz Akhiruddin Maddu Akhmad Endang Zainal Hasan Alimah, Shobiroh Nuur Ambarsavitri, Rahmadhani ANINDA INDRIANI Anja Meryandini Annisa , Alifia Mutiara Annisa Dhiya Athiyyah Khanza Aqilah, Rifany Fairuz Aris Tri Wahyudi Arwansyah Arwansyah Aryani, Hanifah Asrul Muhamad Fuad, Asrul Muhamad Assifah Eryandini Asvarhoza, Genta AYU LESTARI Azhari, Muhammad Alwin Chelsea Chelsea, Chelsea Chelsy Narita Choirunnisa Miftahul Jannah Deki Geraldi Dewi Seswita Zilda Diana Widiastuti DIMAS ANDRIANTO Djarot Sasongko Hami Seno DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Dwi SUBIYARTI Dwicesaria, Maheswari Alfira Dwiningsih Susilowati Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Efi Sanfitri Harahap Elvaza, Azzara Putri Faliha Arinda Lestari Farhan Azhwin Maulana Fathin, Muhammad Faris Fatriani, Rizka Febrianti, Riska Febriyanto, Dimas G Jeni christi A GIA PERMASKU Giovanny, Lisa Gustini Syahbirin H. M. Mochtar Hamidah, Wanda Hanhan Dianhar Harahap, Efi Sanfitri HARTUTIK EKA SUSANTI Hasim - Hasim Hasim Hayati Minarsih Heri Purwoto Hidayat, Radika Evita Hudayanti, Martini Husnawati Husnawati Husnawati, . I MADE ARTIKA I Made Samudera I Made Samudera, I Made I MADE SAMUDRA Ifa Manzila Ifa Manzila Ika Yuni Astutik Ika Yuni Astutik, Ika Yuni Ike Wahyuni Putri Ilham Kurniawan Ilham Kurniawan Imelia Dewi Imron RIYADI Inayah, Mazidah Noer Inda Setyawati, Inda Irma Rahmayani Isnanto, Agung Jaya Hardi Kandi, Rizky Putra Kapitan, Origenes Boy Karichsa Hariana Komalasari, Nurma Angeliani Latifah K Darusman Lestari, Faliha Arinda Liliyani , Ni Putu Peggy Lisa Giovanny Made Suhandana Madyastuti, Rini Marfira, Nurul Mega Safithri Miantika, Shafillah Muhamad Rifai Muhamad Rifai Muhammad Alwin Azhari Muhammad Farhan Muhammad Faris Fathin Muhammad Halim, Muhammad Mustika Luthfia Mustika Permatasari Mustopa, Syahrul Nabila Nur Nafiati Natalia Marbun, Natalia NISA RACHMANIA MUBARIK Nur Qadri Rasyid Nurhidayat Nurhidayat Nurul Marfira Nuur'Alimah, Shobiroh Nuur’Alimah, Shobiroh Pratama, Ahmad Irvan Pratiwi, Anggita Aziz Purwantiningsih Sugita Purwanto, Ukhradiya Magharaniq Safira Purwatiningsih Sugita Puspa Julistia Puspita Putra, Adriansyah Nanda Putri, Ike Wahyuni Radika Evita Hidayah Rahayu, Dyah Utami Cahyaning Rahman, Syabilla Aulia Rasyid, Nur Qadri Ridho Pratama Rifany Fairuz Aqilah Riki Riki Riksa Nur Wahyuni Rini Kurniasih, Rini Rini Novita Riska Febrianti Rosyidah, Rara Annisaur Rury Eryna Putri Sanro Tachibana Sarmila, Sarmila Setyanto Tri Wahyudi Shobiroh Nuur' Alimah Shobiroh Nuur'Alimah Siti Nur'aeni Siti Nurjanah Siti Warnasih Slameut, Fitria Soekarno Mismana Putra Steffanus Gozales Suryani - Suryani Suryani Syamsul Falah TATI NURHAYATI Tirta Setiawan Tony Sumaryada Tri Puji Priyatno Tri Puji Priyatno Uswatun Hasanah Vino , Okta Wahyuni, Riksa Nur Waras Nurcholis Winarti, Novia Yadi Suryadi Yadi Suryadi Yudha, Trah Zahra Silmi Muscifa Zuniar Subastian