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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIEKONOMIKA Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika

Bambang Purwantara

Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor

Author-ID : 240434

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

Published : 49 Documents Claim Missing Document

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INTENSITAS DAN WAKTU ESTRUS PADA BERBAGAI PARITAS INDUK SAPI PERAH FRIES HOLLAND PASCA PARTUS Bambang Hadisutanto; Bambang Purwantara; Siti Darodjah
Partner Vol 19, No 1 (2012): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v19i1.122

Profile Intensity AndÂ Estrus Time In Various Parity Fries Holland Dairy Cattle Postpartum. Parity is a period in the reproductive cycle of cattle with an indication of the amount of carrier confinement livestock.Â Feliciano, et al, (2003) states that the parity is classified into three parts, namely: (1) nuliparous , (2) primiparous and (3) pluriparous / multiparous.This study used one-way classification design with the observed 90 cowsÂ post-partumÂ is composed of 30 first parity, 30 second parityÂ and 30 third parity. Time of estrus observation data were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).This study used one-way classification design with the observed 90 cowsÂ Â post-partumÂ was composed of 30 first parity, 30 second parityÂ and 30 third parity. Data observation ofÂ estrus timeÂ were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).It could be concluded that the parityÂ I, II and III with two estrus intensity (+ +), each for 63.3%, 46.7% and 73.4% and third estrus intensity (+ + +) respectively amounted to 36.6%, 53.3% and 26.6%. While the second estrus postpartum in various parity indicates a difference in means that the parity gives effect to the second estrus postpartum. While the second estrus postpartum in various parity indicated a significance difference that the parity gave effect to the second estrus postpartum.Â Keywords: Fries Holland, Parity, Estrus Intensity, Estrus Time

Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Genetic Diversity of the Endangered Endemic Anoa (Bubalus spp): Implication for Conservation Dwi Sendi Priyono; Dedy Duryadi Solihin; Achmad Farajallah; Bambang Purwantara
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.586-596

Anoa is an endemic ungulate in Sulawesi and its status now is endangered because the population continues to decline. Conservation genetics is one of the crucial issues in the anoa conservation strategy and action plan 2013-2022 document, but this genetic data is not yet available. To investigate and provide valuable information for conservation genetics measures, thirteen polymorphic microsatellites were used to analyze 20 adult anoa. Anoa has relatively low genetic diversity within populations (HO = 0.58), and high genetic differentiation among populations (FST = 0157). Although the anoa population has a bottleneck signal (T.P.M: 0.019; P0.05), the bottleneck simulation results show that the loss of genetic diversity is being slow over the next 100 years (9.5%). We provide some recommendations for conservation genetics based on the findings in this paper, including monitoring and genetically mapping for other anoa populations due to bottleneck signals, establishing the founder of the ex-situ population by examining their genetic diversity status, maintaining and increasing the number of individuals in the ex-situ population to genetically safe population size, and managing anoa populations by avoiding inbreeding. In-situ and ex-situ conservation programs should be combined to maintain the genetic diversity of anoa.

Potensi Alfa Enolase (ENO1) Membran Plasma Spermatozoa Sapi Bali Sebagai protein Antigenik Teguh Sumarsono; Bambang Purwantara; Iman Supriatna; Mohamad Agus Setiadi; Muhammad Agil
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.262-269

Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik.

THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN Oriza Savitri Ariantie; Amrozi Amrozi; Tuty Laswardi Yusuf; Nurul Taufiqu Rochman; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00±330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.00±0.11 and -9.22±0.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90±168.86 nm with PI value 0.34±0.04 and ZP-28.7±1.24 mV. The particle size of freeze-dried egg yolk powder was 1296.00±86.73 nm, with PI value 0.70±0.04 and ZP -34.5±0.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.11±6.06% for 168 hours, compared to FEY-Tris (51.25±6.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.47±6.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.

REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib Gholib; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Michael Heistermann; Antje Engelhardt
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.

PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Faisal Amri Satrio; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin; Asep Kurnia; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.

Sifat bioavailabilitas implan intravaginal berbahan kitosan-PEG-PCL dalam simulasi cairan vagina dan vagina sapi Yessa, Elma Yuliani; Wientarsih, Ietje; Purwantara, Bambang; Amrozi, Amrozi; Ulum, Mokhamad Fakhrul
ARSHI Veterinary Letters Vol. 7 No. 3 (2023): ARSHI Veterinary Letters - August 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.3.57-58

Penelitian ini bertujuan untuk merancang dan mengevaluasi bioavailabilitas implan intravaginal yang terbuat dari poli-ymer kitosan-polietilen glikol (PEG)-polikaprolakton (PCL) yang dapat terbiodegradasi dalam cairan simulasi vagina (SVF) dan vagina sapi. Implan dibuat menggunakan teknik peleburan dan pencetakan dengan 26 formulasi berbeda. Selanjutnya implan direndam dalam 40 mL SVF pada suhu 37,8℃ dan diamati perubahan morfologi implan setiap hari hingga hari ke 5. Implan yang bertahan hingga hari kelima diuji ketersediaannya lebih lanjut pada vagina sapi dan diamati setiap hari selama 2 minggu. Uji perendaman pada hasil SVF menunjukkan bahwa implan dengan kandungan PEG 75-85% lebih cepat meleleh dibandingkan implan dengan kandungan PEG 20-72%. Implan yang mengandung 1-10% PCL terkikis lebih cepat di SVF dibandingkan implan yang mengandung 22-80% PCL. Implan yang mengandung kitosan dapat tetap stabil di SVF lebih lama dibandingkan implan tanpa kitosan. Uji bioavailabilitas pada vagina sapi menunjukkan bahwa implan 21-23 bertahan hingga hari ke 10, sedangkan implan 24-26 dikeluarkan dari vagina sapi selama pengujian. Implan 21-23 merupakan formulasi yang menjanjikan untuk pengembangan implan intravaginal yang dapat terbiodegradasi. Dengan menyesuaikan komposisi kitosan (antara 14-18 berat%), PEG (maksimum 72 berat%), dan PCL (minimal 20 berat%), implan intravaginal mampu meningkatkan ketersediaan hayati cairan vagina dan vagina yang disimulasikan. ternak

The potential of biodegradable polymers: Chitosan, polyethylene glycol, and polycaprolactone as materials for progesterone intravaginal devices Elma Yuliani Yessa; Ietje Wientarsih; Mokhamad Fakhrul Ulum; Bambang Purwantara; Amrozi Amrozi
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.72985

For several decades, a protocol based on the use of progestagens has been used to manage livestock reproduction with minimal alterations. Recently, researchers have gained insight into the short-term use of progestagen protocols lasting 5-7 days, which has been found to reduce the incidence of vaginitis and obviate the use of antibiotics. Additionally, this approach enables the reutilization of silicone-based devices such as CIDRs after a thorough biosecurity assessment. However, these devices have certain limitations. At the end of the treatment, they must be disposed of and cannot be reused, necessitating a re-evaluation of their use for technical and societal reasons, including animal health and welfare, food safety, and environmental impact.A chitosan-PEG intravaginal implant formulation released progesterone for a period of four days, corresponding to the degradation time of the implant in the vagina. The use of a simple melting and molding process for the combination of PCL-PEG-chitosan implants has been observed to result in degradation of both simulated vaginal fluid and vaginal tissue of cows. The development of intravaginal devices made from biodegradable polymers is considered a potential solution because these materials would degrade within the body, eliminating the need for removal and leaving no residue. These devices are safe for animals and the environment.

Co-Authors . Dahri . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Aldridge, Mildred Elizabeth Amrozi Anak Agung Gede Sugianthara Anglin, William Wess Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Bambang Hadisutanto Bambang Hadisutanto BINTAR, ANIFA Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cohn-White, Olivia Lucienne Dechert, Ed Deddy Muchtadi DEDI CANDRA Dewi Suryani Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ekayanti Mulyawati Kaiin Elma Yuliani Yessa Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fearon, Jasmyn Elaine Franks, Brittany Renee Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Giles, Haleigh Margaret GONO SEMIADI Gono Semiadi Gozali Moekti Greene, Ashley Lorraine GREVE, TORBEN H0IER, RENE Hadi S Alikodra Hanby, Riley Dawn Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Holliman, Anna Grace Ietje Wientarsih IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Ketut Sutama Kirby, John Mark Klein, Austin William Kurnia, Asep Kusdiantoro Mohamad Kutchy, Naseer Ahmad Lehmann, Courtney Ann Lies Parede Hernomoadi Llyod, Grace Jennette Lore, Cody Triston LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN McMurray, Tyler B. Memili, Erdogan Michael Heistermann Miller, Hannah Marie Mokhamad Fakhrul Ulum, Mokhamad Moody, Zachary Vinz Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede, Berlin Pandapotan Pickle, Ryan Matthew Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Rutherford, Cobie Sanford, Katie Yvonne Santoso . Santoso Santoso Schaeffer, Laura Marie SCHMiDT, METTE Scott, Rachel Rebecca Seidl, Jeneva Ruth Siti Darodjah Siti Darodjah Rasad Smith, James Dean Sri Darwati SRI RAHAYU Stepp, Hannah Faith Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf WIDJAJA, M. CHANDRA Wike andre Septian Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yeti Lis Purnamadewi Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani

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