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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika

Bambang Purwantara

Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor

Author-ID : 240434

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol Diana Andrianita Kusumaningrum; Bambang Purwantara; Tuti L. Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media. The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Time

Using Artificial Insemination Technology in Goats Surya Natal Tambing; Muhamad Gazali; Bambang Purwantara
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 1 (2001)
Publisher : Indonesian Center for Animal Research and Development

In general, local goat productivity in Indonesia is relatively still lower than that from the temperate area. Yearling body weight in local goat about 14-17 kg. However, the local goat have superiority, such as adaptability and a good reproductive performance, which have litter size 1,49-1,61. Increasing local goat productivity may be done by breeding program (cross breeding and selection) and the environmental improvement. Both cross breeding program and selection that involve mating regulation will be efficient by reproductive technology application, such as artificial insemination (AI). Application of AI in goats is limited by four main factors that influence simultaneously, viz. poor quality of frozen semen, the variety of female reproductive performance, improper technical and insemination time, and poor management. To overcome these constraints his obligatory to pay more attention, so that AI will be able to be used as one of tools to increase the population and genetic quality in goats. Key words: Goat, reproductive efficiency, artificial insemination

PENGARUH FREKUENSI EJAKULASI TERHADAP KARAKTERISTIK SEMEN SEGAR DAN KEMAMPUAN LIBIDO KAMBING SAANEN Surya Natal Tambing; Mozes R. Toelihiere; Tuty L. Yusuf; Bambang Purwantara; Ketut Sutama; Polmer Z. Situmorang
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Sonogram Pemeriksaan Kebuntingan Dini pada Kambing Kacang (Capra hircus) Santoso Santoso; Amrozi Amrozi; Bambang Purwantara; Herdis Herdis
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted to determine the earliest day of pregnancy diagnosis in kacang goat using transrectal ultrasonography. The goat were synchronized by using prostaglandin in the luteal phase. Pregnancy was determined by isoechogenic visualization surrounded by hypoechogenic. Early pregnancy was detected on days 20 of embryonic vesicle diameter 1.2±0.1 cm. Fetus was detected on days 22 with a long gestation fetus 0.4±0.1 cm. Average increase until days 30 pregnancy was 0.19 ± 0.1 cm per day. Development of fetus was followed by an increasing the diameter and thickness of uterus. The diameter of uterus increased from days 14 (0.8 ± 0.3 cm) until days 30 (3.6 ± 0.2 cm), and thickness of uterus increased from days 14 (0.4 ± 0, 2 cm) until days 30 (1.8 ± 0.2 cm). It could be concluded that the earliest pregnancy diagnosis showed positive sign on days 20 and fetus was earliest observed on days 22.

Dinamika Ovarium pada Kuda Hasil Persilangan Pejantan Thoroughbred dengan Induk Lokal Indonesia Muhammad Danang Eko Yulianto; Bambang Purwantara; Amrozi Amrozi
Buletin Peternakan Vol 40, No 3 (2016): BULETIN PETERNAKAN VOL. 40 (3) OKTOBER 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i3.10743

The development of horse breeding industry in Indonesia was commenced through horse racing events held all over the country. It were accelerated by the development of Thoroughbred-Indonesian local Crossbred horses. There are many broodmares injured during their racing time and retired from the racetracks. They may still has a reproductive vigor to continue on producing offsprings. Very little information has been reported on the monitoring the reproductive capacity of the mares. The objective of this study was to explore ultrasonography imaging of the ovarian dynamics, correlated with the estrus behavior of the Thoroughbred-Indonesian local crossbred mares. Three Thoroughbred-Indonesian local crossbred mares with 6.25-12.5% of local genetics aged 12-20 years old were used in this study. Estrus and ovulation were synchronized by 10 mg PGF2α i.m. at luteal phase and 1,500 IU hCG i.m. injection when the dominant follicle reach ≥30 mm in diameter. Ultrasonography examination was done every morning at approximately at the same time. Estrus behavior was observed by using teaser stallions following a standard method. Results of the experiment indicated that onset of the estrus was reached 1.33±0.58 days after the hCG injection, with the average duration of 4.00±1.00 days. The ovulations were done at 5.33±1.15 days after PGF2α treatment and 66.67±10.07 hours after hCG treatment. Maximum follicle diameter was identified to reach 4.50±0.52 cm at one day before ovulation. The mares performed 25.4±3.38 days length of estrus cycle with 2-3 follicular waves. It had been identified that the estrus duration was 6.8±1.92 days in mares with the age of 12-20 years. The average of maximum diameter of the largest follicle before ovulation was 4.2±1.24 cm. In conclusion, to improve the efficiency of breeding, several information are needed i.e. the optimal time of ovulation, relevan parameters related to follicular development.

KUALITAS SEMEN SEGAR SAPI BALI PADA DUA KELOMPOK UMUR DAN PENENTUAN KONSENTRASI KRIOPROTEKTAN DALAM PENGENCER TRIS KUNING TELUR (Fresh Semen Quality Of Bali Bull In Two Group Of Age And Determination Of Cryoprotective Agent Concentration In Tris Eggyolk Extender) Anna Nabilla; Raden Iis Arifiantini; Bambang Purwantara
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different.

Penentuan Siklus Estrus Berdasarkan Gambaran Sitologi Vagina dan Profil Hormon pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Muhammad Rizal; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.983 KB)

Determination of estrous cycle especially the length of estrous is an important role in improvingthe fertility and reproductive performance of farm animals. This study was aimed to observe thelength of estrous cycle and estrous period based on cytologic and estrogen (E2) and progesterone (P4)hormone profiles. Samples of vaginal smears were collected daily and stained with Giemsa. Bloodsamples were collected every two days using jugular vein implant catheter. The hormone profileswere analized by radio immuno assay. All data were analyzed descriptively. Results of this studyshowed that there were four lowest points percentage of parabasal epithelial cells with the averageof 14.25%, while the superficial epithelial cells showed four highest points (85.75%). The intervalsbetween lowest point of parabasal epithelial cell and the highest point of superficial cells were at 20,18, and 16 with the average of 18 days. This phenomenon showed a cyclical processed with almostthe same length of time of the actual an estrous cycle pattern in timor deer. The range of E2 concentration was 7.06 to 18.14 pg/ml and P4 concentration was 2.58 to 7.48 ng/ml. The intervaltime between the peak of E2 was 17 days which represented the estrous cycle of timor deer. It isconcluded that analysis of estrous cycle in timor deer can be detected by vaginal cytological andhormone analysis profiles.

Sinkronisasi Estrus dan Inseminasi Buatan pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara; Gono Semiadi
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.937 KB)

The objective of this experiment was to examine the fertility of timor deer (Rusa timorensis) frozensemen by artificial insemination (AI) with intracervical technique after estrus synchronization withControlled Internal Drug Release, for Goat (CIDR-G®). Six adult, healthy, and cycling hinds aged 3 to 4years were used in this experiment. The percentage of estrus hind, onset and duration of estrus wereobserved. Pregnancy diagnosis was conducted using ultrasound scanner (USG) at day 120 afterinsemination. The response of estrus was 82.22 %, the onset of estrus was 25.33 hours after CIDR®withdrawal with the length of estrus was 28 hours. The pregnancy rate at first AI trial under sedation was16.7% (1/6), at second and third without sedation was 60% (3/5) and 100% (2/2) respectively. The durationof pregnancy was 248 to 285 days; with the average calving rate was 50%. The result concludes thatinsemination in hind without sedation was better than with sedation.

Sonogram Dinamika Ovarium pada Kambing Kacang (Capra hircus) (SONOGRAM OF OVARIAN DYNAMIC IN KACANG GOAT (CAPRA HIRCUS)) Santoso .; Amrozi .; Bambang Purwantara; Herdis .
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.31 KB)

A research was conducted to study the ovarian dynamics in kacang goats. Six non pregnant kacang goatswere used in this research. They were clinically healthy, 2 3 years old and showed normal estrous cycles. Theestrous cycles of the kacang goats were synchronized by using prostaglandin 0.5 mg/kg body weight duringluteal phase. The ovarian dynamics were observed daily by using transrectal ultrasonography. Ovarian dynamicswere characterized by follicular wave. The estrous cycle of kacang goats was 20.0±1.1 days. An ovulatoryfirst follicle wave was appeared on days 1-4, the second follicles wave was on days 3-7, the third folliclewave was on days 8-13, while the ovulatory follicular wave emerged on day 17-18. The dominant folliclesreached the maximum diameter in 3.1±0.6 days from wave emergence and the preovulatory dominantfollicle, the diameter was 6.5±0.5 mm. The corpus luteum of kacang goat was observed on 2.0±0.5 daysafter ovulation up to 2.8±0.8 days before the next ovulation. Follicular development consists of three orfour follicular waves and CL developments were static.

PROFIL GLUKOSA DARAH PADA BERBAGAI PARITAS INDUK SA PI PERAH FRIES HOLLAND PASCA PARTUS Bambang Hadisutanto; . Paggi; Sutarman Muhardja; Siti Darodjah; Bambang Purwantara
Partner Vol 16, No 2 (2009): Edisi November
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v16i2.96

Parity gives an indication of physical maturity of cows. Physical maturity state of primiparous was 82 â€“ 90% while that for pluriparous was 100%. In postpartum period, cows have a negative energy balance. Cows experiencing this condition will use the deposit glycogen in their liver and lipids in adipose tissue to be metabolized by liver become glucose. This experiment aimed at studying blood glucose at different parities of post-partum Fries Holland dairy cows. One-way classification design was use to observe 90 heads of post-partum dairy cows (30 heads of cows each in the first, the second and the third parity state. Data were analyze with General Linear Model (GLM) and the significant difference data among blood glucoses of the cows further analyzed with Duncan Multiple Range Test (DMRT). The results of the study showed that there was a significant (P<0.05) difference between blood glucose of the first parity state of cows (51. 57Â±5. 56 mg/dL) compared to that of the second (45. 57Â±8.01 mg/dL ) and the third (46.7Â±8.62 mg/dL ) cows. This was due to cows in the first parity is in growing state than in producing milk and reproduction function. Cows in the second and the third parities are in opposite function. Blood glucose profile at different parities of post-partum Fries Holland dairy cows was in the normal range (35-55 mg/dL). Blood glucose of the first parity of cows was higher (51.57Â±5.56 mg/dL) compared to that in the second (45.57Â±8.01 mg/dL) and the third cows (46.7Â±8.62 mg/dL). Key words: Fries Holland cows, parity, blood glucose

Co-Authors . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Aldridge, Mildred Elizabeth Amrozi Anak Agung Gede Sugianthara Anglin, William Wess Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Bambang Hadisutanto Bambang Hadisutanto BINTAR, ANIFA Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cohn-White, Olivia Lucienne Dechert, Ed Deddy Muchtadi DEDI CANDRA Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ekayanti Mulyawati Kaiin Elma Yuliani Yessa Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fearon, Jasmyn Elaine Franks, Brittany Renee Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Giles, Haleigh Margaret GONO SEMIADI Gono Semiadi Gozali Moekti Greene, Ashley Lorraine GREVE, TORBEN H0IER, RENE Hadi S Alikodra Hanby, Riley Dawn Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Holliman, Anna Grace Ietje Wientarsih IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Ketut Sutama Kirby, John Mark Klein, Austin William Kurnia, Asep Kusdiantoro Mohamad Kutchy, Naseer Ahmad Lehmann, Courtney Ann Lies Parede Hernomoadi Llyod, Grace Jennette Lore, Cody Triston LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN McMurray, Tyler B. Memili, Erdogan Michael Heistermann Miller, Hannah Marie Mokhamad Fakhrul Ulum, Mokhamad Moody, Zachary Vinz Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede, Berlin Pandapotan Pickle, Ryan Matthew Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Rutherford, Cobie Sanford, Katie Yvonne Santoso . Santoso Santoso Schaeffer, Laura Marie SCHMiDT, METTE Scott, Rachel Rebecca Seidl, Jeneva Ruth Siti Darodjah Siti Darodjah Rasad Smith, James Dean Sri Darwati SRI RAHAYU Stepp, Hannah Faith Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf WIDJAJA, M. CHANDRA Wike andre Septian Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani

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