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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIEKONOMIKA Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Mamangan Social Science Journal Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Bambang Purwantara
Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary Other

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Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol Diana Andrianita Kusumaningrum; Bambang Purwantara; Tuti L. Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.134 KB) | DOI: 10.14334/jitv.v20i4.1240

Abstract

Cryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media.  The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Time
Using Artificial Insemination Technology in Goats Surya Natal Tambing; Muhamad Gazali; Bambang Purwantara
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 1 (2001)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.929 KB) | DOI: 10.14334/wartazoa.v11i1.756

Abstract

In general, local goat productivity in Indonesia is relatively still lower than that from the temperate area. Yearling body weight in local goat about 14-17 kg. However, the local goat have superiority, such as adaptability and a good reproductive performance, which have litter size 1,49-1,61. Increasing local goat productivity may be done by breeding program (cross breeding and selection) and the environmental improvement. Both cross breeding program and selection that involve mating regulation will be efficient by reproductive technology application, such as artificial insemination (AI). Application of AI in goats is limited by four main factors that influence simultaneously, viz. poor quality of frozen semen, the variety of female reproductive performance, improper technical and insemination time, and poor management. To overcome these constraints his obligatory to pay more attention, so that AI will be able to be used as one of tools to increase the population and genetic quality in goats.   Key words: Goat, reproductive efficiency, artificial insemination
PENGARUH FREKUENSI EJAKULASI TERHADAP KARAKTERISTIK SEMEN SEGAR DAN KEMAMPUAN LIBIDO KAMBING SAANEN Surya Natal Tambing; Mozes R. Toelihiere; Tuty L. Yusuf; Bambang Purwantara; Ketut Sutama; Polmer Z. Situmorang
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5378.078 KB) | DOI: 10.22146/jsv.504

Abstract

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Sonogram Pemeriksaan Kebuntingan Dini pada Kambing Kacang (Capra hircus) Santoso Santoso; Amrozi Amrozi; Bambang Purwantara; Herdis Herdis
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.297 KB) | DOI: 10.22146/jsv.27548

Abstract

This study was conducted to determine the earliest day of pregnancy diagnosis in kacang goat using transrectal ultrasonography. The goat were synchronized by using prostaglandin in the luteal phase. Pregnancy was determined by isoechogenic visualization surrounded by hypoechogenic. Early pregnancy was detected on days 20 of embryonic vesicle diameter 1.2±0.1 cm. Fetus was detected on days 22 with a long gestation fetus 0.4±0.1 cm. Average increase until days 30 pregnancy was 0.19 ± 0.1 cm per day. Development of fetus was followed by an increasing the diameter and thickness of uterus. The diameter of uterus increased from days 14 (0.8 ± 0.3 cm) until days 30 (3.6 ± 0.2 cm), and thickness of uterus increased from days 14 (0.4 ± 0, 2 cm) until days 30 (1.8 ± 0.2 cm). It could be concluded that the earliest pregnancy diagnosis showed positive sign on days 20 and fetus was earliest observed on days 22.
Dinamika Ovarium pada Kuda Hasil Persilangan Pejantan Thoroughbred dengan Induk Lokal Indonesia Muhammad Danang Eko Yulianto; Bambang Purwantara; Amrozi Amrozi
Buletin Peternakan Vol 40, No 3 (2016): BULETIN PETERNAKAN VOL. 40 (3) OKTOBER 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i3.10743

Abstract

The development of horse breeding industry in Indonesia was commenced through horse racing events held all over the country. It were accelerated by the development of Thoroughbred-Indonesian local Crossbred horses. There are many broodmares injured during their racing time and retired from the racetracks. They may still has a reproductive vigor to continue on producing offsprings. Very little information has been reported on the monitoring the reproductive capacity of the mares. The objective of this study was to explore ultrasonography imaging of the ovarian dynamics, correlated with the estrus behavior of the Thoroughbred-Indonesian local crossbred mares. Three Thoroughbred-Indonesian local crossbred mares with 6.25-12.5% of local genetics aged 12-20 years old were used in this study. Estrus and ovulation were synchronized by 10 mg PGF2α i.m. at luteal phase and 1,500 IU hCG i.m. injection when the dominant follicle reach ≥30 mm in diameter. Ultrasonography examination was done every morning at approximately at the same time. Estrus behavior was observed by using teaser stallions following a standard method. Results of the experiment indicated that onset of the estrus was reached 1.33±0.58 days after the hCG injection, with the average duration of 4.00±1.00 days. The ovulations were done at 5.33±1.15 days after PGF2α treatment and 66.67±10.07 hours after hCG treatment. Maximum follicle diameter was identified to reach 4.50±0.52 cm at one day before ovulation. The mares performed 25.4±3.38 days length of estrus cycle with 2-3 follicular  waves. It had been identified that the estrus duration  was  6.8±1.92  days  in mares with the age of 12-20 years. The average of maximum diameter of the largest follicle before ovulation was 4.2±1.24 cm. In conclusion, to improve the efficiency of breeding, several information are needed i.e. the optimal time of ovulation, relevan parameters related to follicular development. 
PROFIL GLUKOSA DARAH PADA BERBAGAI PARITAS INDUK SA PI PERAH FRIES HOLLAND PASCA PARTUS Bambang Hadisutanto; . Paggi; Sutarman Muhardja; Siti Darodjah; Bambang Purwantara
Partner Vol 16, No 2 (2009): Edisi November
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v16i2.96

Abstract

Parity gives an indication of physical maturity of cows. Physical maturity state of primiparous was 82 – 90% while that for pluriparous was 100%. In postpartum period, cows have a negative energy balance. Cows experiencing this condition will use the deposit glycogen in their liver and lipids in adipose tissue to be metabolized by liver become glucose. This experiment aimed at studying blood glucose at different parities of post-partum Fries Holland dairy cows. One-way classification design was use to observe 90 heads of post-partum dairy cows (30 heads of cows each in the first, the second and the third parity state. Data were analyze with General Linear Model (GLM) and the significant difference data among blood glucoses of the cows further analyzed with Duncan Multiple Range Test (DMRT). The results of the study showed that there was a significant (P<0.05) difference between blood glucose of the first parity state of cows (51. 57±5. 56 mg/dL) compared to that of the second (45. 57±8.01 mg/dL ) and the third (46.7±8.62 mg/dL ) cows. This was due to cows in the first parity is in growing state than in producing milk and reproduction function. Cows in the second and the third parities are in opposite function. Blood glucose profile at different parities of post-partum Fries Holland dairy cows was in the normal range (35-55 mg/dL). Blood glucose of the first parity of cows was higher (51.57±5.56 mg/dL) compared to that in the second (45.57±8.01 mg/dL) and the third cows (46.7±8.62 mg/dL). Key words: Fries Holland cows, parity, blood glucose
INTENSITAS DAN WAKTU ESTRUS PADA BERBAGAI PARITAS INDUK SAPI PERAH FRIES HOLLAND PASCA PARTUS Bambang Hadisutanto; Bambang Purwantara; Siti Darodjah
Partner Vol 19, No 1 (2012): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v19i1.122

Abstract

Profile Intensity And  Estrus Time In Various Parity Fries Holland Dairy Cattle Postpartum. Parity is a period in the reproductive cycle of cattle with an indication of the amount of carrier confinement livestock.  Feliciano, et al, (2003) states that the parity is classified into three parts, namely: (1) nuliparous , (2) primiparous and (3) pluriparous / multiparous.This study used one-way classification design with the observed 90 cows  post-partum  is composed of 30 first parity, 30 second parity  and 30 third parity. Time of estrus observation data were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).This study used one-way classification design with the observed 90 cows   post-partum  was composed of 30 first parity, 30 second parity  and 30 third parity. Data observation of  estrus time  were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).It could be concluded that the parity  I, II and III with two estrus intensity (+ +), each for 63.3%, 46.7% and 73.4% and third estrus intensity (+ + +) respectively amounted to 36.6%, 53.3% and 26.6%. While the second estrus postpartum in various parity indicates a difference in means that the parity gives effect to the second estrus postpartum. While the second estrus postpartum in various parity indicated a significance difference that the parity gave effect to the second estrus postpartum. Keywords: Fries Holland, Parity, Estrus Intensity, Estrus Time
Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Abstract

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.
Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Abstract

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.
Genetic Diversity of the Endangered Endemic Anoa (Bubalus spp): Implication for Conservation Dwi Sendi Priyono; Dedy Duryadi Solihin; Achmad Farajallah; Bambang Purwantara
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.586-596

Abstract

Anoa is an endemic ungulate in Sulawesi and its status now is endangered because the population continues to decline. Conservation genetics is one of the crucial issues in the anoa conservation strategy and action plan 2013-2022 document, but this genetic data is not yet available. To investigate and provide valuable information for conservation genetics measures, thirteen polymorphic microsatellites were used to analyze 20 adult anoa. Anoa has relatively low genetic diversity within populations (HO = 0.58), and high genetic differentiation among populations (FST = 0157). Although the anoa population has a bottleneck signal (T.P.M: 0.019; P0.05), the bottleneck simulation results show that the loss of genetic diversity is being slow over the next 100 years (9.5%). We provide some recommendations for conservation genetics based on the findings in this paper, including monitoring and genetically mapping for other anoa populations due to bottleneck signals, establishing the founder of the ex-situ population by examining their genetic diversity status, maintaining and increasing the number of individuals in the ex-situ population to genetically safe population size, and managing anoa populations by avoiding inbreeding. In-situ and ex-situ conservation programs should be combined to maintain the genetic diversity of anoa.
Co-Authors . Dahri . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Aldridge, Mildred Elizabeth Amrozi Anak Agung Gede Sugianthara Anglin, William Wess Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Bambang Hadisutanto Bambang Hadisutanto BINTAR, ANIFA Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cohn-White, Olivia Lucienne Dechert, Ed Deddy Muchtadi DEDI CANDRA Dewi Suryani Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ekayanti Mulyawati Kaiin Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fearon, Jasmyn Elaine Franks, Brittany Renee Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Giles, Haleigh Margaret GONO SEMIADI Gono Semiadi Gozali Moekti Greene, Ashley Lorraine GREVE, TORBEN H0IER, RENE Hadi S Alikodra Hanby, Riley Dawn Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Holliman, Anna Grace IETJE WIENTARSIH Iman Supriatna Irida Novianti ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Ketut Sutama Kirby, John Mark Klein, Austin William Kurnia, Asep Kusdiantoro Mohamad Kutchy, Naseer Ahmad Lehmann, Courtney Ann Lies Parede Hernomoadi Llyod, Grace Jennette Lore, Cody Triston LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN McMurray, Tyler B. Memili, Erdogan Michael Heistermann Miller, Hannah Marie Mokhamad Fakhrul Ulum, Mokhamad Moody, Zachary Vinz Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede, Berlin Pandapotan Pickle, Ryan Matthew Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Rutherford, Cobie Sanford, Katie Yvonne Santoso . Santoso Santoso Schaeffer, Laura Marie SCHMiDT, METTE Scott, Rachel Rebecca Seidl, Jeneva Ruth Siti Darodjah Siti Darodjah Rasad Smith, James Dean Sri Darwati SRI RAHAYU Stepp, Hannah Faith Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF WIDJAJA, M. CHANDRA Wike andre Septian Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yeti Lis Purnamadewi Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani