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RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE RAFFIUDIN, RIKA; BINTAR, ANIFA; WIDJAJA, M. CHANDRA; FARAJALLAH, AHMAD; PURWANTARA, BAMBANG
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.086 KB) | DOI: 10.11598/btb.2009.16.1.65

Abstract

Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable for rapid preparation of DNA from A. mellifera by the Animal Quarantine facility. Results showed that all 94 colony samples from beekeepers in Java produced a 485 bp PCR product from the amplification of this gene. Two DNA fragments of 194 and 291 bp from all samples were produced after digestion with BglII. This cytb/BglII result together with the DNA sequence of cyt b showed that all collected samples of A. mellifera were the non-AHB type. Hence, this study did not detect AHB in Indonesia.Key words :  Apis mellifera, molecular detection methods, DNA, cytochrome b, mitochondrial genetics
PREOVULATORY CHANGES AND OVULATION IN CATTLE UNDERGOING SPONTANEOUS OR CLOPROSTENOL-INDUCED LUTEOLYSIS PURWANTARA, BAMBANG; H0IER, RENE; SCHMiDT, METTE; GREVE, TORBEN
BIOTROPIA Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.683 KB) | DOI: 10.11598/btb.2006.13.2.223

Abstract

The follicular population, diameter of the ovulatory and subordinate follicles, corpus luteum (CL) size, concentration of progesterone and estradiol-17p were studied following spontaneous or cloprostenol-induced luteolysis. A total of 14 heifers received cloprostenol treatment on Day 9-11 of the cycle to synchronize their estrus. Subsequently, they were divided into two groups, one group which was allowed to undergo spontaneous luteolysis and the other group in which estrus was induced from days 9 to 12. In the induced-group, transrectal ultrasonography were performed daily started two days prior to injection until the onset of estrus. In the spontaneous-group, ultrasonography was done daily from day 15 until the onset of estrus. In both groups scanning were performed every 4 h from the onset of estrus until ovulation was ascertained. Small (SF, 2-4 mm), medium (MF.5-9 mm ) and large (LF,>9mm) size follicles were recorded. The diameter of largest and subordinate follicles were measured and blood were drawn from jugular vein at approximately around scanning and the plasma were used for measurement of progesterone (P4) and estradiol-17p (E2) concentration. There was no different in term of number of SF, MF and LF (P>0.05) between the two groups. Similarly, no effect of side (left vs. right ovary) and CL position (ipsi- vs contralateral to the ovary) was found (P>0.05). However, it was demonstrated that mean number of ovulatory follicles was higher (PO.01) in the spontaneously ovulating group while the regressing-CL size was larger in the cloprostenol induced animals (P
REVIEW: SOLUTIONS FOR GRAND CHALLENGES IN GOAT AND SHEEP PRODUCTION INDUSTRY Aldridge, Mildred Elizabeth; Fearon, Jasmyn Elaine; Haynes, Bethany Peyton; Miller, Hannah Marie; Sanford, Katie Yvonne; Scott, Rachel Rebecca; Anglin, William Wess; Blalock, Lauren Susanne; Burkes, Briana Lashun; Cohn-White, Olivia Lucienne; Franks, Brittany Renee; Giles, Haleigh Margaret; Greene, Ashley Lorraine; Hanby, Riley Dawn; Holliman, Anna Grace; Kirby, John Mark; Klein, Austin William; Lehmann, Courtney Ann; Llyod, Grace Jennette; Lore, Cody Triston; McMurray, Tyler B.; Moody, Zachary Vinz; Palmer, Brianna Nicole; Pansano, Lindsey Virginia; Pickle, Ryan Matthew; Schaeffer, Laura Marie; Seidl, Jeneva Ruth; Smith, James Dean; Stepp, Hannah Faith; Satrio, Faisal Amri; Kutchy, Naseer Ahmad; Dechert, Ed; Rutherford, Cobie; Brown, Kipp; Purwantara, Bambang; Memili, Erdogan
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.511 KB) | DOI: 10.11598/btb.2019.26.1.944

Abstract

Goats and sheep are valuable as they are a source of meat, milk, fleece, and other products. These livestock are also important both for agriculture and biomedical research. However, the efficient, sustainable, and profitable production of these small ruminants faces major obstacles. Hence, this review analyzes these major challenges specifically, their negative impacts on the industry, and suggests some science-based solutions to overcome them. Those challenged areas are education and training, research, translational research/biotechnology, goat and sheep health, and maintenance of an economically sustainable agribusiness. The suggested solutions include the effective teaching of goat and sheep science to the next generation and public empowerment, support for innovative and translational research, disease prevention and treatment, support for technology transfer, and development of sound agribusiness practices. This review is helpful particularly for scientists, students, and the goat and sheep producers. In general, these information on the current state of goat and sheep agriculture will also help the public to better understand and appreciate the challenges met and opportunities provided in small ruminant production enterprises.
Knowledge, perception, and public attitude on biotech crops in Indonesia Tanjung, Dahri; Purnamadewi, Yeti Lis; Purwantara, Bambang; Suryani, Dewi
Agriekonomika Vol 14, No 1: April 2025
Publisher : Department of Agribusiness, Faculty of Agriculture, Universitas Trunojoyo Madura, Indonesi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agriekonomika.v14i1.28237

Abstract

Until now, many genetically engineered products have met our basic needs and provided various benefits. However, there are still many who doubt or even reject them. This raises the question of how the public's knowledge and understanding of these biotechnological plants stand. This research aims to analyze whether public actions in adopting biotechnology are influenced by their knowledge and perceptions. This survey used a questionnaire with 535 respondents. The results show that about 70% of respondents have limited knowledge about biotechnology, with information channels including print media, social media, and interpersonal communication. About 75% of respondents agree that biotechnology can enhance agricultural productivity, the economy, and farmers' welfare, as well as contribute to food security. Furthermore, 76% of respondents agree to purchase these products. PLS-SEM analysis reveals a strong positive relationship between knowledge, perceptions, and positive attitudes toward biotechnology products. Therefore, enhancing information dissemination through various alternative media and involving stakeholders need to be strengthened in the future.
Knowledge, Perception, and Public Attitude on Biotech Crops in Indonesia Tanjung, Dahri; Purnamadewi, Yeti Lis; Purwantara, Bambang; Suryani, Dewi
Jurnal Mamangan Vol 12, No 2 (2023): Special Issue
Publisher : LPPM Universitas PGRI Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22202/mamangan.v12i2.10002

Abstract

Until now, many genetically engineered products have met our basic needs and provided various economic, environmental, and health benefits. However, there are still many who doubt or even oppose them. This raises the question of how the public's knowledge and understanding of these biotechnological plants stand. Consequently, this condition will affect the varied attitudes of society. Thus, this research aims to analyze whether public actions in adopting biotechnology are significantly influenced by their knowledge and perceptions. This survey uses a questionnaire and was conducted with 535 respondents from several provinces. The results show that around 70% of respondents have limited knowledge about biotechnology. The information channels they use include print media, social media, and interpersonal communication. About 75% of respondents agree or strongly agree that biotechnology can enhance agricultural productivity, the economy, and farmers' welfare, and contribute to food security. Furthermore, 76% of respondents agree to purchase these products. Despite the above findings, the lack of information regarding genetically engineered plants has led a majority of Indonesians to feel "neutral" about the risks they perceive regarding health and the environment. PLS-SEM analysis reveals a strong positive relationship between knowledge, perceptions, and positive attitudes toward biotechnology products. Therefore, factors such as enhancing information dissemination through various alternative media and involving stakeholders need to be intensified for the future development of biotechnology
The potential of biodegradable polymers: Chitosan, polyethylene glycol, and polycaprolactone as materials for progesterone intravaginal devices Yessa, Elma Yuliani; Wientarsih, Ietje; Ulum, Mokhamad Fakhrul; Purwantara, Bambang; Amrozi, Amrozi
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.72985

Abstract

For several decades, a protocol based on the use of progestagens has been used to manage livestock reproduction with minimal alterations. Recently, researchers have gained insight into the short-term use of progestagen protocols lasting 5-7 days, which has been found to reduce the incidence of vaginitis and obviate the use of antibiotics. Additionally, this approach enables the reutilization of silicone-based devices such as CIDRs after a thorough biosecurity assessment. However, these devices have certain limitations. At the end of the treatment, they must be disposed of and cannot be reused, necessitating a re-evaluation of their use for technical and societal reasons, including animal health and welfare, food safety, and environmental impact.A chitosan-PEG intravaginal implant formulation released progesterone for a period of four days, corresponding to the degradation time of the implant in the vagina. The use of a simple melting and molding process for the combination of PCL-PEG-chitosan implants has been observed to result in degradation of both simulated vaginal fluid and vaginal tissue of cows. The development of intravaginal devices made from biodegradable polymers is considered a potential solution because these materials would degrade within the body, eliminating the need for removal and leaving no residue. These devices are safe for animals and the environment.
Co-Authors . Dahri . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Aldridge, Mildred Elizabeth Amrozi Anak Agung Gede Sugianthara Anglin, William Wess Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Bambang Hadisutanto Bambang Hadisutanto BINTAR, ANIFA Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cohn-White, Olivia Lucienne Dechert, Ed Deddy Muchtadi DEDI CANDRA Dewi Suryani Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ekayanti Mulyawati Kaiin Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fearon, Jasmyn Elaine Franks, Brittany Renee Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Giles, Haleigh Margaret Gono Semiadi GONO SEMIADI Gozali Moekti Greene, Ashley Lorraine GREVE, TORBEN H0IER, RENE Hadi S Alikodra Hanby, Riley Dawn Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Holliman, Anna Grace IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Ketut Sutama Kirby, John Mark Klein, Austin William Kurnia, Asep Kusdiantoro Mohamad Kutchy, Naseer Ahmad Lehmann, Courtney Ann Lies Parede Hernomoadi Llyod, Grace Jennette Lore, Cody Triston LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN McMurray, Tyler B. Memili, Erdogan Michael Heistermann Miller, Hannah Marie Mokhamad Fakhrul Ulum, Mokhamad Moody, Zachary Vinz Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede, Berlin Pandapotan Pickle, Ryan Matthew Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Rutherford, Cobie Sanford, Katie Yvonne Santoso . Santoso Santoso Schaeffer, Laura Marie SCHMiDT, METTE Scott, Rachel Rebecca Seidl, Jeneva Ruth Siti Darodjah Siti Darodjah Rasad Smith, James Dean Sri Darwati SRI RAHAYU Stepp, Hannah Faith Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf WIDJAJA, M. CHANDRA Wike andre Septian Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yeti Lis Purnamadewi Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani