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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Bambang Purwantara
Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia Purwantara, Bambang; Pardede, Berlin Pandapotan; Agil, Muhammad; Supriatna, Iman; Karja, Ni Wayan Kurniani; Arifiantini, R. Iis; Sumantri, Cece
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0404.367-374

Abstract

Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.
Display of fresh semen quality, frozen semen production, and field fertility of Bali bulls (Bos sondaicus) in different season Fatmila, Dian Tria; Yudi; Purwantara, Bambang; Hasan, Fuad; Sutanto, Wahyudi Himawan
Indonesian Journal of Agricultural Research Vol. 8 No. 2 (2025): InJAR, Vol. 8, No. 2, July 2025
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/injar.v8i2.20096

Abstract

Bali cattle is one of Indonesia's local cattle that has high economic potential. The quality of the semen used plays an important role as a factor in the success of artificial insemination (AI) programs. Climate change, including temperature variations, rainfall, and length of solar exposure, can affect the quality of fresh semen produced by bulls. Differences in environmental conditions can affect semen characteristics, such as spermatozoa concentration, motility, and morphology, which in turn have an impact on the field fertility rate. The aim of this study is to explore the differences in male fertility based on environmental conditions that change throughout the seasons. The research method used in this study is non-experimental, using secondary data on the quality of fresh semen and frozen semen production collected from the Regional AI Center, Bali Province. In addition, this study also used data from the big data of the National Animal Health Information System (iSIKHNAS) to calculate field fertility represented by conception rate. Climate data was obtained from the Indonesian Agency for Meteorological, Climatological and Geophysics (BMKG) Bali Province, the data collected was climate data in Tabanan Regency. The differences in the appearance of fresh semen quality, frozen semen production, and field fertility were analyzed using the Independent T-Test. In this study, there was no significant difference in fresh semen quality, frozen semen production, and field fertility (P>0.05) in all parameters, but the average reproductive performance of bali bulls was higher in the rainy season than in the dry season.
PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Satrio, Faisal Amri; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus; Kaiin, Ekayanti Mulyawati; Kurnia, Asep; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23487

Abstract

This study aimed to determine the effect of age on bulls productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, 10 yo with high semen rejection (10 HR), and 10 yo with low semen rejection (10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of 10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in 10 yo groups. The 10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of 10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in 10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN Ariantie, Oriza Savitri; Amrozi, Amrozi; Yusuf, Tuty Laswardi; Rochman, Nurul Taufiqu; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.19669

Abstract

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.000.11 and -9.220.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90168.86 nm with PI value 0.340.04 and ZP-28.71.24 mV. The particle size of freeze-dried egg yolk powder was 1296.0086.73 nm, with PI value 0.700.04 and ZP-34.50.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.116.06% for 168 hours, compared to FEY-Tris (51.256.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.476.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.
REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib, Gholib; Agil, Muhammad; Supriatna, Iman; Purwantara, Bambang; Heistermann, Michael; Engelhardt, Antje
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.6830

Abstract

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.
RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE RAFFIUDIN, RIKA; BINTAR, ANIFA; WIDJAJA, M. CHANDRA; FARAJALLAH, AHMAD; PURWANTARA, BAMBANG
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.086 KB) | DOI: 10.11598/btb.2009.16.1.65

Abstract

Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable for rapid preparation of DNA from A. mellifera by the Animal Quarantine facility. Results showed that all 94 colony samples from beekeepers in Java produced a 485 bp PCR product from the amplification of this gene. Two DNA fragments of 194 and 291 bp from all samples were produced after digestion with BglII. This cytb/BglII result together with the DNA sequence of cyt b showed that all collected samples of A. mellifera were the non-AHB type. Hence, this study did not detect AHB in Indonesia.Key words :  Apis mellifera, molecular detection methods, DNA, cytochrome b, mitochondrial genetics
PREOVULATORY CHANGES AND OVULATION IN CATTLE UNDERGOING SPONTANEOUS OR CLOPROSTENOL-INDUCED LUTEOLYSIS PURWANTARA, BAMBANG; H0IER, RENE; SCHMiDT, METTE; GREVE, TORBEN
BIOTROPIA Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.683 KB) | DOI: 10.11598/btb.2006.13.2.223

Abstract

The follicular population, diameter of the ovulatory and subordinate follicles, corpus luteum (CL) size, concentration of progesterone and estradiol-17p were studied following spontaneous or cloprostenol-induced luteolysis. A total of 14 heifers received cloprostenol treatment on Day 9-11 of the cycle to synchronize their estrus. Subsequently, they were divided into two groups, one group which was allowed to undergo spontaneous luteolysis and the other group in which estrus was induced from days 9 to 12. In the induced-group, transrectal ultrasonography were performed daily started two days prior to injection until the onset of estrus. In the spontaneous-group, ultrasonography was done daily from day 15 until the onset of estrus. In both groups scanning were performed every 4 h from the onset of estrus until ovulation was ascertained. Small (SF, 2-4 mm), medium (MF.5-9 mm ) and large (LF,>9mm) size follicles were recorded. The diameter of largest and subordinate follicles were measured and blood were drawn from jugular vein at approximately around scanning and the plasma were used for measurement of progesterone (P4) and estradiol-17p (E2) concentration. There was no different in term of number of SF, MF and LF (P>0.05) between the two groups. Similarly, no effect of side (left vs. right ovary) and CL position (ipsi- vs contralateral to the ovary) was found (P>0.05). However, it was demonstrated that mean number of ovulatory follicles was higher (PO.01) in the spontaneously ovulating group while the regressing-CL size was larger in the cloprostenol induced animals (P
Review: Solutions for Grand Challenges in Goat and Sheep Production Industry Aldridge, Mildred Elizabeth; Fearon, Jasmyn Elaine; Haynes, Bethany Peyton; Miller, Hannah Marie; Sanford, Katie Yvonne; Scott, Rachel Rebecca; Anglin, William Wess; Blalock, Lauren Susanne; Burkes, Briana Lashun; Cohn-White, Olivia Lucienne; Franks, Brittany Renee; Giles, Haleigh Margaret; Greene, Ashley Lorraine; Hanby, Riley Dawn; Holliman, Anna Grace; Kirby, John Mark; Klein, Austin William; Lehmann, Courtney Ann; Llyod, Grace Jennette; Lore, Cody Triston; McMurray, Tyler B.; Moody, Zachary Vinz; Palmer, Brianna Nicole; Pansano, Lindsey Virginia; Pickle, Ryan Matthew; Schaeffer, Laura Marie; Seidl, Jeneva Ruth; Smith, James Dean; Stepp, Hannah Faith; Satrio, Faisal Amri; Kutchy, Naseer Ahmad; Dechert, Ed; Rutherford, Cobie; Brown, Kipp; Purwantara, Bambang; Memili, Erdogan
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.511 KB) | DOI: 10.11598/btb.2019.26.1.944

Abstract

Goats and sheep are valuable as they are a source of meat, milk, fleece, and other products. These livestock are also important both for agriculture and biomedical research. However, the efficient, sustainable, and profitable production of these small ruminants faces major obstacles. Hence, this review analyzes these major challenges specifically, their negative impacts on the industry, and suggests some science-based solutions to overcome them. Those challenged areas are education and training, research, translational research/biotechnology, goat and sheep health, and maintenance of an economically sustainable agribusiness. The suggested solutions include the effective teaching of goat and sheep science to the next generation and public empowerment, support for innovative and translational research, disease prevention and treatment, support for technology transfer, and development of sound agribusiness practices. This review is helpful particularly for scientists, students, and the goat and sheep producers. In general, these information on the current state of goat and sheep agriculture will also help the public to better understand and appreciate the challenges met and opportunities provided in small ruminant production enterprises.
Co-Authors . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Aldridge, Mildred Elizabeth Amrozi Anak Agung Gede Sugianthara Anglin, William Wess Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Bambang Hadisutanto Bambang Hadisutanto BINTAR, ANIFA Blalock, Lauren Susanne Brown, Kipp Burkes, Briana Lashun Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cohn-White, Olivia Lucienne Dechert, Ed Deddy Muchtadi DEDI CANDRA Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ekayanti Mulyawati Kaiin Elma Yuliani Yessa Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fearon, Jasmyn Elaine Franks, Brittany Renee Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Giles, Haleigh Margaret GONO SEMIADI Gono Semiadi Gozali Moekti Greene, Ashley Lorraine GREVE, TORBEN H0IER, RENE Hadi S Alikodra Hanby, Riley Dawn Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Holliman, Anna Grace Ietje Wientarsih IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Ketut Sutama Kirby, John Mark Klein, Austin William Kurnia, Asep Kusdiantoro Mohamad Kutchy, Naseer Ahmad Lehmann, Courtney Ann Lies Parede Hernomoadi Llyod, Grace Jennette Lore, Cody Triston LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN McMurray, Tyler B. Memili, Erdogan Michael Heistermann Miller, Hannah Marie Mokhamad Fakhrul Ulum, Mokhamad Moody, Zachary Vinz Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Palmer, Brianna Nicole Pansano, Lindsey Virginia Pardede, Berlin Pandapotan Pickle, Ryan Matthew Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Rutherford, Cobie Sanford, Katie Yvonne Santoso . Santoso Santoso Schaeffer, Laura Marie SCHMiDT, METTE Scott, Rachel Rebecca Seidl, Jeneva Ruth Siti Darodjah Siti Darodjah Rasad Smith, James Dean Sri Darwati SRI RAHAYU Stepp, Hannah Faith Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf WIDJAJA, M. CHANDRA Wike andre Septian Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani