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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Bambang Purwantara
Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Faisal Amri Satrio; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin; Asep Kurnia; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.363 KB) | DOI: 10.21157/j.ked.hewan.v16i1.23487

Abstract

This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN Oriza Savitri Ariantie; Amrozi Amrozi; Tuty Laswardi Yusuf; Nurul Taufiqu Rochman; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.19669

Abstract

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00±330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.00±0.11 and -9.22±0.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90±168.86 nm with PI value 0.34±0.04 and ZP-28.7±1.24 mV. The particle size of freeze-dried egg yolk powder was 1296.00±86.73 nm, with PI value 0.70±0.04 and ZP -34.5±0.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.11±6.06% for 168 hours, compared to FEY-Tris (51.25±6.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.47±6.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.
PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Faisal Amri Satrio; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin; Asep Kurnia; Bambang Purwantara
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23487

Abstract

This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib Gholib; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Michael Heistermann; Antje Engelhardt
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.6830

Abstract

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.
Sifat bioavailabilitas implan intravaginal berbahan kitosan-PEG-PCL dalam simulasi cairan vagina dan vagina sapi Yessa, Elma Yuliani; Wientarsih, Ietje; Purwantara, Bambang; Amrozi, Amrozi; Ulum, Mokhamad Fakhrul
ARSHI Veterinary Letters Vol. 7 No. 3 (2023): ARSHI Veterinary Letters - August 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.3.57-58

Abstract

Penelitian ini bertujuan untuk merancang dan mengevaluasi bioavailabilitas implan intravaginal yang terbuat dari poli-ymer kitosan-polietilen glikol (PEG)-polikaprolakton (PCL) yang dapat terbiodegradasi dalam cairan simulasi vagina (SVF) dan vagina sapi. Implan dibuat menggunakan teknik peleburan dan pencetakan dengan 26 formulasi berbeda. Selanjutnya implan direndam dalam 40 mL SVF pada suhu 37,8℃ dan diamati perubahan morfologi implan setiap hari hingga hari ke 5. Implan yang bertahan hingga hari kelima diuji ketersediaannya lebih lanjut pada vagina sapi dan diamati setiap hari selama 2 minggu. Uji perendaman pada hasil SVF menunjukkan bahwa implan dengan kandungan PEG 75-85% lebih cepat meleleh dibandingkan implan dengan kandungan PEG 20-72%. Implan yang mengandung 1-10% PCL terkikis lebih cepat di SVF dibandingkan implan yang mengandung 22-80% PCL. Implan yang mengandung kitosan dapat tetap stabil di SVF lebih lama dibandingkan implan tanpa kitosan. Uji bioavailabilitas pada vagina sapi menunjukkan bahwa implan 21-23 bertahan hingga hari ke 10, sedangkan implan 24-26 dikeluarkan dari vagina sapi selama pengujian. Implan 21-23 merupakan formulasi yang menjanjikan untuk pengembangan implan intravaginal yang dapat terbiodegradasi. Dengan menyesuaikan komposisi kitosan (antara 14-18 berat%), PEG (maksimum 72 berat%), dan PCL (minimal 20 berat%), implan intravaginal mampu meningkatkan ketersediaan hayati cairan vagina dan vagina yang disimulasikan. ternak
The potential of biodegradable polymers: Chitosan, polyethylene glycol, and polycaprolactone as materials for progesterone intravaginal devices Elma Yuliani Yessa; Ietje Wientarsih; Mokhamad Fakhrul Ulum; Bambang Purwantara; Amrozi Amrozi
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.72985

Abstract

For several decades, a protocol based on the use of progestagens has been used to manage livestock reproduction with minimal alterations. Recently, researchers have gained insight into the short-term use of progestagen protocols lasting 5-7 days, which has been found to reduce the incidence of vaginitis and obviate the use of antibiotics. Additionally, this approach enables the reutilization of silicone-based devices such as CIDRs after a thorough biosecurity assessment. However, these devices have certain limitations. At the end of the treatment, they must be disposed of and cannot be reused, necessitating a re-evaluation of their use for technical and societal reasons, including animal health and welfare, food safety, and environmental impact.A chitosan-PEG intravaginal implant formulation released progesterone for a period of four days, corresponding to the degradation time of the implant in the vagina. The use of a simple melting and molding process for the combination of PCL-PEG-chitosan implants has been observed to result in degradation of both simulated vaginal fluid and vaginal tissue of cows. The development of intravaginal devices made from biodegradable polymers is considered a potential solution because these materials would degrade within the body, eliminating the need for removal and leaving no residue. These devices are safe for animals and the environment.
Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia Purwantara, Bambang; Pardede, Berlin Pandapotan; Agil, Muhammad; Supriatna, Iman; Karja, Ni Wayan Kurniani; Arifiantini, R. Iis; Sumantri, Cece
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0404.367-374

Abstract

Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.
Display of fresh semen quality, frozen semen production, and field fertility of Bali bulls (Bos sondaicus) in different season Fatmila, Dian Tria; Yudi; Purwantara, Bambang; Hasan, Fuad; Sutanto, Wahyudi Himawan
Indonesian Journal of Agricultural Research Vol. 8 No. 2 (2025): InJAR, Vol. 8, No. 2, July 2025
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/injar.v8i2.20096

Abstract

Bali cattle is one of Indonesia's local cattle that has high economic potential. The quality of the semen used plays an important role as a factor in the success of artificial insemination (AI) programs. Climate change, including temperature variations, rainfall, and length of solar exposure, can affect the quality of fresh semen produced by bulls. Differences in environmental conditions can affect semen characteristics, such as spermatozoa concentration, motility, and morphology, which in turn have an impact on the field fertility rate. The aim of this study is to explore the differences in male fertility based on environmental conditions that change throughout the seasons. The research method used in this study is non-experimental, using secondary data on the quality of fresh semen and frozen semen production collected from the Regional AI Center, Bali Province. In addition, this study also used data from the big data of the National Animal Health Information System (iSIKHNAS) to calculate field fertility represented by conception rate. Climate data was obtained from the Indonesian Agency for Meteorological, Climatological and Geophysics (BMKG) Bali Province, the data collected was climate data in Tabanan Regency. The differences in the appearance of fresh semen quality, frozen semen production, and field fertility were analyzed using the Independent T-Test. In this study, there was no significant difference in fresh semen quality, frozen semen production, and field fertility (P>0.05) in all parameters, but the average reproductive performance of bali bulls was higher in the rainy season than in the dry season.
Co-Authors . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Amrozi Anak Agung Gede Sugianthara ANIFA BINTAR Anna Grace Holliman Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Asep Kurnia Ashley Lorraine Greene Austin William Klein Bambang Hadisutanto Bambang Hadisutanto Bethany Peyton Haynes Briana Lashun Burkes Brianna Nicole Palmer Brittany Renee Franks Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cobie Rutherford Cody Triston Lore Courtney Ann Lehmann Deddy Muchtadi DEDI CANDRA Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ed Dechert Ekayanti Mulyawati Kaiin Elma Yuliani Yessa Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Erdogan Memili Faisal Amri Satrio Fajar Basuki fatmila, dian tria Fransiska R Zakaria Fuad Hasan Gholib Gholib Gono Semiadi GONO SEMIADI Gozali Moekti Grace Jennette Llyod Hadi S Alikodra Haleigh Margaret Giles Hannah Faith Stepp Hannah Marie Miller HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Ietje Wientarsih IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA James Dean Smith Jasmyn Elaine Fearon Jeneva Ruth Seidl John Mark Kirby Katie Yvonne Sanford Ketut Sutama Kipp Brown Kusdiantoro Mohamad Laura Marie Schaeffer Lauren Susanne Blalock Lies Parede Hernomoadi Lindsey Virginia Pansano LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. CHANDRA WIDJAJA M. Zairin Junior MADE ASTAWAN METTE SCHMiDT Michael Heistermann Mildred Elizabeth Aldridge Mokhamad Fakhrul Ulum, Mokhamad Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Naseer Ahmad Kutchy Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Olivia Lucienne Cohn-White Oloan Parlindungan Oriza Savitri Ariantie Pardede, Berlin Pandapotan Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rachel Rebecca Scott RENE H0IER Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Riley Dawn Hanby Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Ryan Matthew Pickle Santoso . Santoso Santoso Siti Darodjah Siti Darodjah Rasad Sri Darwati SRI RAHAYU Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono TORBEN GREVE Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tyler B. McMurray Wike andre Septian William Wess Anglin Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani Zachary Vinz Moody