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Abundance and Distribution of Millipedes (Diplopoda) in Wera Nature Recreation Park Rifana Lemba; Syech Zainal; Rafiqa Rafiqa; Fatmah Dhafir; Masrianih Masrianih; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.597-603

Abstract

Millipedes (Diplopoda) play an important role in forest ecosystems, particularly in the decomposition of organic matter and the recycling of nutrients, thereby contributing to soil fertility and ecosystem stability. This study aimed to determine the abundance and distribution patterns of millipedes (Diplopoda) in Wera Nature Recreation Park, Balumpewa Village, West Dolo District, Sigi Regency, Central Sulawesi. The research was designed as a quantitative descriptive study. Sampling was carried out using an exploratory survey method combined with hand collection techniques at three observation stations. These stations were selected using purposive sampling based on differences in environmental characteristics and habitat conditions. Data collection was conducted in three repetitions at two-week intervals to obtain representative data on millipede communities. In addition to species collection, measurements of physical environmental parameters were also performed, including light intensity, soil pH, air temperature, and humidity, as these factors are known to influence the presence and distribution of soil macrofauna. The results showed that six millipede species were recorded, belonging to three orders and three families. The average environmental conditions at the study site included a light intensity of 722.2 lux, soil pH of 6.8, temperature of 28.2°C, and humidity of 76.7%. These conditions indicate a habitat that is relatively less optimal for millipede populations. The species abundance index was classified as low, suggesting limited population sizes within the study area. However, the Relative Abundance Index (RAI) revealed variation among species, with two species categorized as having high relative abundance, one species moderate, and three species low. Analysis using Morisita’s Index and the Standardized Morisita Index (Ip) demonstrated that all recorded species exhibited a clumped distribution pattern. This distribution suggests that millipedes tend to concentrate in specific microhabitats that provide suitable moisture, shelter, and food resources. Overall, the findings indicate that unstable environmental conditions may restrict millipede distribution, causing them to utilize only particular microhabitats that meet their ecological requirements.
Effects of Red Coconut Tembuluk (Cocos nucifera var. rubescens) Extract on Uric Acid Level Reduction in Mice (Mus musculus) Sukmawati Sukmawati; Sutrisnawati Sutrisnawati; Manap Trianto; Masrianih Masrianih; Fatmah Dhafir; I Nengah Kundera
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.693-698

Abstract

Increased levels of uric acid in the blood can lead to metabolic disorders such as gouty arthritis, which require effective management. This study aimed to determine the effect of red coconut tembuluk extract (Cocos nucifera var. rubescens) on reducing uric acid levels in mice (Mus musculus). The study employed an experimental research design using a Completely Randomized Design (CRD). A total of 28 mice were used, divided into seven groups with four replications each. The groups consisted of a normal control group (KN), which received food and water ad libitum; a negative control group (K+); a positive control group (K-); and four treatment groups (PI, PII, PIII, and PIV). The negative control group (K+), positive control group (K-), and all treatment groups were induced with lard oil for 14 days to increase uric acid levels. The positive control group (K?) was treated with allopurinol at a dose of 0.026 mg per 20 g body weight. The normal control (KN) and negative control (K+) groups received food and water ad libitum without extract administration. Treatment group PI received 10% red coconut tembuluk extract, PII received 20%, PIII received 40%, and PIV received 80%. The data obtained were analyzed using Analysis of Variance (ANOVA). The results showed that administration of red coconut tembuluk extract significantly reduced uric acid levels in mice induced with lard oil. The most effective concentration in reducing uric acid levels was 80% (PIV), with an average uric acid level of 5.4 mg/dL.
Types and Abundance of Microplastics in African Catfish (Clarias gariepinus) from Sumber Agung Village, Parigi Moutong Mudrikah Tus Sa'diah; Fatmah Dhafir; Masrianih; Sutrisnawati; Bustamin; Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20493

Abstract

This study aimed to determine the types and abundance of microplastics in African catfish (Clarias gariepinus) collected from aquaculture ponds and a river in Sumber Agung Village. A descriptive research design was employed using both qualitative and quantitative approaches. A total of 10 catfish aged approximately two months were used as samples, consisting of five individuals from aquaculture ponds and five individuals from the river. Digestive tract samples were digested using 10% KOH solution for 96 hours to separate microplastic particles, followed by filtration, identification, and particle counting. The results showed that microplastics were detected in all catfish samples. Three types of microplastics were identified: fibers, films, and fragments. The abundance of microplastics in catfish from aquaculture ponds was 103.6 particles/individual, which was higher than that observed in catfish from the river, at 57.6 particles/individual. Fragments were the dominant microplastic type in pond-derived catfish, whereas fibers predominated in river-derived catfish. Differences in microplastic abundance were influenced by the characteristics of the aquatic environment, with ponds having limited water circulation that may facilitate microplastic accumulation. These findings indicate that catfish have the potential to serve as bioindicators of microplastic pollution in both aquaculture systems and natural freshwater environments. However, microplastic identification in this study was based solely on microscopic observation; therefore, the polymer composition of the detected microplastics could not be confirmed.
Analysis of Microplastics in the Digestive Tracts of Mackerel Tuna (Euthynnus affinis) and Skipjack Tuna (Katsuwonus pelamis) for SDG 14 Masriani Masriani; Abd. Hakim Laenggeng; Fatmah Dhafir; Aan Febriawan
Journal of Current Studies in SDGs Vol. 2 No. 1 (2026): March
Publisher : Sekolah Tinggi Agama Islam Sabilul Muttaqin Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63230/jocsis.2.1.157

Abstract

Objective: Marine ecosystems are increasingly threatened by microplastic pollution, which can accumulate in commercially important fish species and potentially affect human health. This study aimed to identify the characteristics and abundance of microplastics in the digestive tracts of mackerel tuna (Euthynnus affinis) and skipjack tuna (Katsuwonus pelamis) from Boneoge waters, Donggala Regency, and examine the relationship between digestive tract size and microplastic abundance. Method: A quantitative descriptive approach was employed using 30 specimens of each fish species collected from local fishermen. Digestive tract samples were digested using 10% KOH, incubated at 75°C, filtered, and observed under a binocular microscope. Microplastic abundance was expressed as particles per gram of digestive tract weight. Simple linear regression analysis using SPSS 25 was performed to evaluate the relationship between digestive tract size and microplastic abundance. Results: Three microplastic types were identified: film, fragment, and fiber, with film being dominant in both species. Total microplastic abundance reached 3.940 particles/g in mackerel tuna and 3.071 particles/g in skipjack tuna. Regression analysis showed a weak positive relationship between digestive tract size and microplastic abundance (R² = 0.137 and 0.140), suggesting that other environmental and biological factors play a greater role. Novelty: Providing the first baseline data on microplastic contamination in mackerel tuna and skipjack tuna from Boneoge waters, Central Sulawesi, and contributes to SDG 14 (Life Below Water) by strengthening evidence-based efforts to address marine microplastic pollution.
Analysis of Microplastics in the Digestive Tracts of Mackerel Tuna (Euthynnus affinis) and Skipjack Tuna (Katsuwonus pelamis) for SDG 14 Masriani Masriani; Abd. Hakim Laenggeng; Fatmah Dhafir; Aan Febriawan
Journal of Current Studies in SDGs Vol. 2 No. 1 (2026): March
Publisher : Sekolah Tinggi Agama Islam Sabilul Muttaqin Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63230/jocsis.2.1.157

Abstract

Objective: Marine ecosystems are increasingly threatened by microplastic pollution, which can accumulate in commercially important fish species and potentially affect human health. This study aimed to identify the characteristics and abundance of microplastics in the digestive tracts of mackerel tuna (Euthynnus affinis) and skipjack tuna (Katsuwonus pelamis) from Boneoge waters, Donggala Regency, and examine the relationship between digestive tract size and microplastic abundance. Method: A quantitative descriptive approach was employed using 30 specimens of each fish species collected from local fishermen. Digestive tract samples were digested using 10% KOH, incubated at 75°C, filtered, and observed under a binocular microscope. Microplastic abundance was expressed as particles per gram of digestive tract weight. Simple linear regression analysis using SPSS 25 was performed to evaluate the relationship between digestive tract size and microplastic abundance. Results: Three microplastic types were identified: film, fragment, and fiber, with film being dominant in both species. Total microplastic abundance reached 3.940 particles/g in mackerel tuna and 3.071 particles/g in skipjack tuna. Regression analysis showed a weak positive relationship between digestive tract size and microplastic abundance (R² = 0.137 and 0.140), suggesting that other environmental and biological factors play a greater role. Novelty: Providing the first baseline data on microplastic contamination in mackerel tuna and skipjack tuna from Boneoge waters, Central Sulawesi, and contributes to SDG 14 (Life Below Water) by strengthening evidence-based efforts to address marine microplastic pollution.
Total Flavonoid and Tannin Content in Pumpkin Stem Extract (Cucurbita moschata) Vadillah Vadillah; Sutrisnawati Sutrisnawati; Manap Trianto; Fatmah Dhafir; Zulaikhah Dwi Jayanti; Masrianih Masrianih
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1113-1119

Abstract

Cucurbita moschata is a medicinal plant that possesses various pharmacological properties, including antidiabetic, antifungal, antibacterial, anti-inflammatory, and antioxidant activities, which are associated with its bioactive compound content. One plant part that has been relatively underutilized is the pumpkin stem, although it has the potential to contain secondary metabolites such as flavonoids and tannins. This study aimed to identify and determine the levels of bioactive compounds in pumpkin stem extract based on stem maturity stages, namely young stems and mature stems, using both qualitative and quantitative methods. Qualitative analysis was conducted through phytochemical screening using color reaction methods with specific reagents to detect flavonoids and tannins. Quantitative analysis was performed using UV-Vis spectrophotometry based on standard calibration curves. The results showed that pumpkin stem extract tested positive for both flavonoids and tannins. Quantitative analysis indicated that the flavonoid content in young stems was 7.22, which was higher than in mature stems (3.00). In contrast, tannin content was higher in mature stems (9.03) compared to young stems (5.02). These differences indicate that flavonoids are more dominant in young tissues with active metabolic activity, whereas tannins tend to accumulate in mature tissues as part of plant defense mechanisms. Based on these findings, it can be concluded that pumpkin stem extract contains flavonoid and tannin compounds with varying levels depending on stem maturity, suggesting its potential as a natural source of antioxidants.
Population Density and Distribution Pattern of Golden Apple Snail (Pomacea canaliculata L.) in the Rice Field Ecosystem Sinta Sinta; Sutrisnawati Sutrisnawati; Manap Trianto; Masrianih Masrianih; Rafiqah Rafiqah; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1121-1127

Abstract

The golden apple snail (Pomacea canaliculata) is a freshwater mollusc species commonly found in rice field ecosystems and is recognized as one of the major pests of rice crops. This species possesses a high adaptive capacity and a rapid reproductive rate, allowing its population to increase rapidly and cause significant damage to rice plants. Nevertheless, the golden apple snail also has ecological roles and potential benefits, including serving as an alternative feed source for livestock and fish, as well as contributing to the decomposition of organic matter in aquatic environments. This study aimed to analyze the population density and distribution pattern of the golden apple snail in the rice fields of Dolago Padang Village, South Parigi District. The research employed a quantitative descriptive approach using a field survey method. Data were collected using a quadrat sampling technique with plot sizes of 2 × 2 m established at three different observation stations. The number of golden apple snail individuals within each plot was counted directly and subsequently analyzed to determine population density and distribution patterns using the Morisita Index. In addition, environmental parameters, including water temperature and pH, were measured. The results showed that a total of 90 golden apple snail individuals were recorded during the study. Population density ranged from 1 to 2 individuals/m², which was categorized as moderate. The distribution pattern of the golden apple snail at all observation stations was classified as uniform, as indicated by Morisita Index values below 1 (Id < 1). Environmental conditions, with temperatures ranging from 27–30°C and pH values between 6.5 and 7.2, were within the optimal range supporting the presence and development of the golden apple snail.
Diversity of Butterfly (Suborder: Rhopalocera) in the Kapopo Grand Forest Park Area, Central Sulawesi Murni Ammalia; Abd Hakim Laenggeng; Manap Trianto; Masrianih Masrianih; Amalia Buntu; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1079-1086

Abstract

Butterflies are important components of biodiversity, serving as pollinators and environmental bioindicators. This study aimed to assess butterfly diversity (Suborder Rhopalocera) in the Kapopo Grand Forest Park, Central Sulawesi, Indonesia. A quantitative descriptive approach was employed using purposive sampling across three observation stations representing high-vegetation, low-vegetation, and riverine habitats. Data were collected through field surveys conducted during morning and afternoon observation periods, and species were identified based on morphological characteristics. Species diversity was analyzed using the Shannon–Wiener diversity index. A total of 26 butterfly species belonging to three families Nymphalidae, Pieridae, and Papilionidae were recorded, comprising 323 individuals. Nymphalidae was the most species-rich family with 13 species, followed by Pieridae (7 species) and Papilionidae (6 species). The Shannon–Wiener diversity index (H') was 1.5, indicating a moderate level of species diversity (1 <= H' <= 3). This result suggests that habitat conditions within the Kapopo Grand Forest Park remain relatively stable and suitable for sustaining butterfly populations. Variations in habitat characteristics among observation stations, particularly differences in vegetation structure and levels of human disturbance, influenced the distribution and abundance of butterfly species throughout the study area.
Nest Associated Mites (Acari: Winterschmidtiidae) of Stingless Bee Tetragonula fuscobalteata in Central Sulawesi, Indonesia Nurly Maftukhah; I Made Budiarsa; Manap Trianto; Masrianih Masrianih; Rafiqah Rafiqah; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1265-1271

Abstract

Stingless bees are essential pollinators in tropical ecosystems and play an important role in sustaining biodiversity and agricultural productivity. In Central Sulawesi, Indonesia, Tetragonula fuscobalteata is widely cultivated in small-scale meliponiculture systems. However, information regarding mites associated with its nests remains limited. This study aimed to identify and describe nest-associated mites found in T. fuscobalteata colonies. The research was conducted in Dolago Village, Parigi Moutong Regency, Central Sulawesi. Nest samples were collected from five active colonies. Mites were observed under a stereo microscope, collected using a fine brush, and mounted on slides with polyvinyl alcohol. Identification was based on morphological characteristics using standard taxonomic keys, and morphometric measurements were obtained using Image Raster 3.0. All specimens were identified as deutonymphs of the genus Saproglyphus (Acari: Winterschmidtiidae). Mites were found in storage pots and cerumen structures but were absent from brood cells. The deutonymphs exhibited a well-developed sucker plate, reduced chelicerae, and fused coxae III, indicating phoretic adaptation. The mean body length was 192.72 ± 22.14 µm and width was 145.78 ± 12.62 µm. These findings provide baseline data on mite associations in T. fuscobalteata nests and contribute to understanding arthropod interactions in meliponiculture systems.
Diversity of Insect Species on Bird's Eye Chili Pepper (Capsicum frutescens L.) in Tudua Village, Morowali Regency Nurul Amna Moh Said; Fatmah Dhafir; Syech Zainal; Manap Trianto; Sutrisnawati Sutrisnawati; Masrianih Masrianih
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1223-1229

Abstract

Insects are the most diverse group of living organisms, accounting for nearly 80% of all known animal species. They belong to the phylum Arthropoda and exhibit a wide range of morphological characteristics. Insects can be found in almost all habitats, ranging from lush tropical rainforests to harsh deserts, as well as aquatic environments such as rivers and lakes. This study aimed to identify and describe insect species associated with bird's eye chili pepper (Capsicum frutescens L.) in Tudua Village. The research was conducted in October 2025. The study sites were selected using purposive sampling, with three observation plots measuring 10 × 10 m each. Insect samples were collected using a combination of sweeping nets, pitfall traps, light traps, and yellow traps. The collected specimens were identified based on their morphological characteristics up to the order, family, genus, or species level and were analyzed descriptively to determine their ecological roles within the agroecosystem. The results revealed a total of 29 insect species belonging to eight orders: Araneae, Coleoptera, Hemiptera, Orthoptera, Odonata, Lepidoptera, Hymenoptera, and Blattodea. The identified insects exhibited diverse ecological roles, including pests, herbivores, predators, pollinators, and decomposers. Members of the order Hemiptera and some species of Coleoptera were the most commonly encountered pest groups. Meanwhile, Araneae, Hymenoptera, and Odonata functioned as natural predators, Lepidoptera acted as pollinators, and Blattodea served as decomposers. The presence and distribution of insects were influenced by environmental factors such as temperature, humidity, light intensity, and soil pH.
Co-Authors Aan Febriawan Abd Hakim Laenggeng Abd Hakim Laenggeng, Abd Hakim Abd Rauf Abd Rauf Abd Rauf Abd. Hakim Laenggeng Abd. Hakim Laenggeng Abd. Hakim Laenggeng, Abd. Hakim Abd. Hakim Langgeng Abd. Rauf Abd. Rauf Abdul Ashari Abdul Hakim Achmad - Ramadhan, Achmad - Achmad Ramadhan Achmad Ramadhan Achmad Ramadhan Achmad, Mestawaty As. Adi Fahri Agreista Toripa Agustin Tiwow Amalia Buntu Amelia A Riuh Amelia Sry Utami Amiruddin Kasim Amran Rede Amran Rede Andi Mamas Andi Tanra Tellu Annisa, Rezky Artha, Fandy Ashari, Abdul Ayu Rifka Ramadani Burhan Burhan, Burhan Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin D, Mutiara Datubaringan, Jein David Pebriyanto Tulak Dawa, Linda M Delima, Nur Devi Devi Dewi Tureni Erna, Ni Kadek Estin Nursanti Handayani Fadhilah Fadhilah Faradila, Ratna Febriawan, Aan Felisia Talantu Firga Feliana, Firga Gamar Binti Non Shamdas Hadijah S. Pago Hayyatun Mawaddah Henny Chandra Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Iin B Mantulangi Ika Istadewi Illah, Ininna Nur Isnainar Isnainar Isnainar Isnainar Isninar, Isnainar Kaltisa, Kaltisa Laksono Trisnantoro Latompai, Nismawati I. Lestari M. P. Alibasyah Lestari M.P. Alibasyah Lilies Lilies Lusiana - - Manap Trianto MARIA BINTANG Maria Ulpa Djuanda Masriani . Masriani Masriani Masrianih Masrianih Masrianih Mestawaty As. Achmad Mestawaty Mestawaty Minarni Rama Jura Moh Dhuhri Surya Wirawan Mohammad Jamhari Mudrikah Tus Sa'diah Murnadi, Ni Kade Murni Ammalia Mursito S Bialangi Mursito S. Bialangi Musdalifah Nurdin Musdayanti Musdayanti Muslika Nifatun Umaya Najma AR. Talamoa Ni Kadek Apriyani Ni Kadek Deladarmi Yanti Ni Luh Indrianti Nismawati I. Latompai Novriadi Novriadi Nuning Niwana Nur Hikmah Nuraini Nuraini Nurly Maftukhah Nurrahma Jumaliani Nursam Nursam Nurul Amna Moh Said Nurwahda, Nurwahda Permatasari, Ni Kadek Dewi Puspitasari, Amira Putri, Adelia Putri, Aticha Anata Putu Maharani Rafiqa Rafiqa Rafiqa Rafiqa RAFIQA RAFIQA, RAFIQA Rafiqah Rafiqah Rahmadhani, Lili Ratnawathi, Ni Wayan Raya Agni Rezkiana, Sry Ayu Rifana Lemba Rizkia Khairunnisa Safitri, Gusti Ayu Kadek Yunita Salbia Salbia Samsurizal, M. Sulaeman Silvia Agustin Sinta Sinta Sitti Nur Fadhilah Suardi, Magfira Sukmawati Sukmawati SULFIANTI, SULFIANTI Suprianto Suprianto Suprianto Suprianto Suprianto Sutrisna Sutrisna Sutrisnawati Mardin Sutrisnawati Sutrisnawati Syaifuloh, Syaifullah Syech Zainal Syech Zainal Tulak, David Pebriyanto Uzaini Nur Rahma Vadillah Vadillah Vita Indri Febriani Windarsih, Yulia Yusril Ihza Mahendra Yusril Ihza Mahendra Zulaikhah Dwi Jayanti Zulkaidah - -, Zulkaidah -