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Struktur 3D Protein Struktural VP1 pada Enterovirus A71 Menggunakan Swiss-Model Suprianto Suprianto; Made Budiarsa; Fatmah Dhafir
BIOEDUSCIENCE Vol 4 No 1 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/j.bes/4137-474353

Background: Protein struktural VP1 berperan sebagai pemain kunci dalam patogenesis, memiliki keunikan yang cukup menarik untuk dikaji dengan mempelajari sifat dan fungsi protein struktural VP1. Penelitian ini bertujuan untuk memprediksi struktur tiga dimensi protein VP1 pada EV-A71. Metode: Protein target diperoleh dari server UniProt dengan kode akses A0A097EV89 menggunakan template 4cey.1.A (PDB ID) dianalisis secara in silico melalui metode homologi menggunakan server SWISS-MODEL. Hasil: analisis penelitian menunjukkan protein target dan template memiliki identity 95,29 % dan tersusun dari 297 asam amino dengan nilai QMEAN -2,15. Protein struktural VP1 pada Ramachandran Plots memiliki struktur stabil, residu non-glisin pada daerah outlier hanya berkisar 0,34 % (A53 ALA) dengan Nilai rotamer outliers 1,61 %. Kesimpulan: Model struktur tiga dimensi protein yang diteliti memiliki struktur stabil dan informasi yang didapatkan berguna untuk penelitian lebih lanjut dalam pengembangan vaksin penyakit yang disebabkan oleh EV-A71.

Variasi Morfometrik Tetragonula laeviceps di Berbagai Habitat di Provinsi Sulawesi Tengah I Made Budiarsa; Fatmah Dhafir; Manap Trianto
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/625062

Background: Habitat is a place for living things to grow and develop. Differences in habitat in an area can affect the size of an organism. This study aims to determine the morphometric variations of Tetragonula laeviceps found in urban and forest habitats in Central Sulawesi Province. Methods: This research was conducted with roaming technique. Morphometric characters observed were 35 characters of stingless bee and analyzed using Microsoft Excel software. Results: The results of this study indicated that the morphometric size variation of T. laeviceps found in forest habitats (3.58 mm) is greater than that of wild bee in urban habitats (3.05 mm). Conclusions: Different habitat characteristics can affect the morphometry variations of the bee, especially in T. laeviceps species. The bee's morphometry body size is greater than in the forest habitat, because in that habitat there are environmental temperatures and types of plant sources of nectar which are more supportive for bee life compared to urban habitats.

Flying Activity of Apis cerana Honey Bee at Surouding Hive Area in Central Sulawesi I Made Budiarsa; Masrianih; Fatmah Dhafir; Manap Trianto
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5440

Information on foraging activity is important for efforts to increase the productivity of Apis cerana honeybees. Honey bees flying activities are carried out in the context of nest guarding activities from enemy attacks, removing feces from the nest, and foraging which takes place during the day to collect pollen and nectar from plants. This study aims to analyze the daily activity patterns of bees A. cerana which is influenced by environmental factors. This study used two colonies of A. cerana, namely colony one and colony two with the same colony size. The observed behaviors were bees leaving the nest, entering with pollen, and entering without pollen. The number of A. cerana colony one flying for pollen was high in the morning and low in the afternoon due to the very high light intensity during the day. Unlike colony one, A. cerana colony two did not actively perform flight activities and showed a different activity of flying in front of the nest forming a crowd in the afternoon. An inflorescence calendar needs to be developed to determine the flowering period of plants around the hive so that bee forage is continuously available to avoid absconding. absconding. Identification of plant pollen as a source of bee food needs to be done to determine the types of plants utilized by bees.

Visiting of Carpenter Bees Xylocopa latipes Drury to The Bean Crop in Central Sulawesi Masrianih Masrianih; I Made Budiarsa; Fatmah Dhafir; Manap Trianto
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5453

Xylocopa latipes Drury are one species of bee found in the Hymenoptera order, in the family Anthophoridae. Carpenter bees, which are essential for plant pollination, build their nests in decaying wood and bark. Carpenter bees visit the bean crop to collect pollen and nectar for their hives. This study aims to determine the visit of the wood bee X. latipes on long bean plants in Central Sulawesi. The study employed a descriptive survey methodology that involved field sampling at bean planting and direct observation of X. latipes in the field. measured the temperature and the light intensity. The findings revealed that X. latipes visits to the bean crop at Desa Nambaru, Kecamatan Parigi Selatan, Kabupaten Parigi Moutong average 58,3 people each visit. The morning at 08.30 am averages 21 X. latipes visits; the afternoon at 15.30 pm averages 9 Xylocopa latipe visits; and the afternoon at 11.30 am averages 2 Xylocopa latipe visits. The amount of light present during X. latipes visit to the bean crop has a significant impact. Weather variables like temperature, humidity, and wind speed had an impact on the multivariate regression's very real relationship to X. latipes visit to bean planting.

Frugivorous Flies on Bitter Gourd Fruit in Parigi Moutong Regency Fatmah Dhafir; Manap Trianto
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4b.5854

In Indonesia, many people eat pariah as a vegetable. This plant can not only be consumed as a vegetable, but can also be used for medical purposes. Sample collection was carried out using purposive sampling technique. Samples taken were pariah fruits suspected of being infested by frugivorous fly pests. Sampling was done once in four different fields for each commodity. There were five types of frugivorous flies found on peria fruit, namely Z. cucurbitae, Z. calumniatus, Z. tau, A. Orientalis, and flies of the family Lonchaeidae species Silba sp. The highest proportion of flies found from peria fruit was Z. cucurbitae. The highest single percentage infestation of flies on peria fruit was A. orientalis. A. orientalis has the potential to infest fruit singly on peria fruit because it has the highest percentage of single infestation compared to other frugivorous flies. Parasitoids found were Psyttalia sp. and Fopius arisanus. Further research needs to be done on the single infestation of A. orientalis and Lonchaeidae, especially on peria fruit by inserting the flies into caged fruit at different maturity levels and varieties, so that it can be proven whether A. orientalis and Lonchaeidae can infest fruit singly on peria fruit and cucumber and how to control them so that the population level can be suppressed.

Struktur 3D Protein Struktural VP1 pada Enterovirus A71 Menggunakan Swiss-Model Suprianto Suprianto; Made Budiarsa; Fatmah Dhafir
BIOEDUSCIENCE Vol 4 No 1 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/j.bes/4137-474353

Background: Protein struktural VP1 berperan sebagai pemain kunci dalam patogenesis, memiliki keunikan yang cukup menarik untuk dikaji dengan mempelajari sifat dan fungsi protein struktural VP1. Penelitian ini bertujuan untuk memprediksi struktur tiga dimensi protein VP1 pada EV-A71. Metode: Protein target diperoleh dari server UniProt dengan kode akses A0A097EV89 menggunakan template 4cey.1.A (PDB ID) dianalisis secara in silico melalui metode homologi menggunakan server SWISS-MODEL. Hasil: analisis penelitian menunjukkan protein target dan template memiliki identity 95,29 % dan tersusun dari 297 asam amino dengan nilai QMEAN -2,15. Protein struktural VP1 pada Ramachandran Plots memiliki struktur stabil, residu non-glisin pada daerah outlier hanya berkisar 0,34 % (A53 ALA) dengan Nilai rotamer outliers 1,61 %. Kesimpulan: Model struktur tiga dimensi protein yang diteliti memiliki struktur stabil dan informasi yang didapatkan berguna untuk penelitian lebih lanjut dalam pengembangan vaksin penyakit yang disebabkan oleh EV-A71.

Variasi Morfometrik Tetragonula laeviceps di Berbagai Habitat di Provinsi Sulawesi Tengah I Made Budiarsa; Fatmah Dhafir; Manap Trianto
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/625062

Background: Habitat is a place for living things to grow and develop. Differences in habitat in an area can affect the size of an organism. This study aims to determine the morphometric variations of Tetragonula laeviceps found in urban and forest habitats in Central Sulawesi Province. Methods: This research was conducted with roaming technique. Morphometric characters observed were 35 characters of stingless bee and analyzed using Microsoft Excel software. Results: The results of this study indicated that the morphometric size variation of T. laeviceps found in forest habitats (3.58 mm) is greater than that of wild bee in urban habitats (3.05 mm). Conclusions: Different habitat characteristics can affect the morphometry variations of the bee, especially in T. laeviceps species. The bee's morphometry body size is greater than in the forest habitat, because in that habitat there are environmental temperatures and types of plant sources of nectar which are more supportive for bee life compared to urban habitats.

Study of NTF-3 (Neurotrophic Factor 3) Gene Information in Columbidae by In Silico Suprianto, S.; Budiarsa, I Made; Dhafir, Fatmah; Permatasari, Ni Kadek Dewi; Safitri, Gusti Ayu Kadek Yunita
BERKALA SAINSTEK Vol 9 No 3 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i3.24163

Neurotrophin Factor 3 (NTF3) is one of the genes that plays an important role in the regulation of the neural systems of vertebrate animals, this gene has a special function in explaining the survival factors of some vertebrate animals. Based on the information obtained from GenBank, the nucleotide sequence of the NTF-3 gene in several vertebrate animals has been known and some of the data obtained have not been studied further for research purposes in adding information related to the molecular character of the NTF-3 gene, such as the NTF-3 gene in Columbidae. Columbidae is a group of birds that have quite diverse species variations, the number of species in columbidae will be very helpful in obtaining data on comparisons of the genetic character of the NTF3 gene. The purpose of this study was to analyze and describe the information on the NTF-3 gene (Neurotrophic Factor 3) in Columbidae through the in silico approach with computational methods. The NTF3 gene nucleotide sequences in Columbidae showed a fairly high level of similarity to the base sequences. This illustrates the fairly close proximity between each species. Geotrygon Montana is a species of Columbidae which has variations of the Base sequence which is quite different from other species. Evaluation of the model structure shows good stability of each target protein, all evaluation results describe a good structure, meaning that the conformation of each target sequence is in accordance with the sequence, so that the structure that is built has high accuracy with the actual model. The results of this research study can be a special description in explaining the genetic characteristics of several Columbidae species for the purposes of conservation measures or efforts to preserve Columbidae species at the molecular and population genetic levels.

Pelatihan Pembuatan Bahan Ajar Digital bagi Guru Mata Pelajaran di SMP Karuna Dipa Palu Febriawan, Aan; Hakim, Abdul; Lilies; Dhafir, Fatmah
Jurnal Abdidas Vol. 5 No. 5 (2024): October 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v5i5.1031

Inovasi merupakan tindakan yang perlu dilakukan secara terus menerus demi menunjang kemajuan khususnya di dunia pendidikan. Langkah ini dipandang sebagai salah satu faktor penentu dalam keberhasilan masyarakat. Kemajuan teknologi saat ini memberikan tantangan tersendiri di kalangan pengajar. Kegiatan pengabdian ini bertujuan untuk memberi pemahaman dan keterampilan kepada guru mata pelajaran di SMP Karuna Dipa Palu terkait pembuatan bahan ajar digital sebagai bentuk pengembangan diri dan inovasi yang dapat dilakukan oleh guru mata pelajaran sebagai respon menghadapi era revolusi industri 4.0 saat ini. Kegiatan ini diikuti oleh guru mata pelajaran sejumlah kurang lebih 15 orang. Metode yang digunakan dalam kegiatan adalah pelatihan diawali dengan penyampaian materi terlebih dahulu oleh narasumber lalu dilanjutkan dengan praktik dalam membuat bahan ajar digital menggunakan software Flip PDF Profesional. Pihak SMP Karuna Dipa Palu berharap agar kegiatan yang serupa dapat terus dilakukan, untuk terus memberi informasi terkini dalam mengembangkan keterampilan guru agar dapat terus beradaptasi dengan teknologi yang terus berkembang saat ini.

Pengaruh Lama Waktu Fermentasi Terhadap Kandungan Protein Olahan Tempe Biji Kamonji (Artocarpus camansi) dan Pemanfaatannya sebagai Media Pembelajaran Fadhilah; Dhafir, Fatmah; Masrianih
Journal of Biology Science and Education Vol. 6 No. 2 (2018): Desember
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jbse.v6i2.1062

Selama ini tempe berasal dari fermentasi kacang kedelai dengan menggunakan Rhizopus spp. Tempe yang biasa ditemukan di pasar-pasar berbahan dasar kedelai, sehingga harga kedelai makin mahal. Biji kamonji dapat dimanfaatkan sebagai bahan alternatif untuk pembuatan tempe, karena mudah didapatkan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh waktu fermentasi terhadap kandungan protein dari olahan tempe biji kamonji (Artocarpus camansi) dan pemanfaatannya sebagai media pembelajaran. Adapun metode yang dilakukan dalam penelitian ini yaitu menggunakan meode RAL. Analisa yang dilakukan adalah Analisis Varian (ANAVA) dan diuji menggunakan uji Beda Nyata Terkecil (BNT) dengan taraf signifikan 0,5%. Untuk analisis protein mrnggunakan metode Kjedhal. Lama waktu fermentasi yang digunakan yaitu 12 jam (P1), 24 jam (P2) dan 36 jam ( P3). Hasil penelitian menunjukkan bahwa dari ketiga variasi lama waktu fermentasi yang digunakan memberikan pengaruh terhadap kandungan protein dari olahan tempe biji kamonji. semakin lama waktu fermentasinya maka kandungan protein yang dihasilkan juga semakin tinggi. Kandungan protein yang dihasilkan dari ketiga lama waktu fermentasi yaitu 12 jam (P1) sebesar 11,10%, 24 jam (P2) sbesar 13,36% dan kandungan protein yang paling tinggi ditunjukkan oleh tempe biji kamonji lama waktu fermentasi selama 36 jam (P3) dengan kandungan protein sebesar 14,37%. Hasil validasi media pembelajaran menunjukkan hasil Penelitian ini sangat layak digunakan sebagai media pembelajaran dalam bentuk poster.

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