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Journal : Bioscience

The Effect of Coffee Arabica (Coffea arabica L.) Fruit Skin Extracts on Small Intestine Morphometry of mice (Mus musculus L.) with Ethanol-Induced Rita Maliza; Febriofca Galih Yatalaththov; Haris Setiawan; Listiatie Budi Utami
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111571-0-00

Abstract

Consumption of alcoholic beverages can increase ROS and inflammation. In the duodenal, the consumption of alcohol can result in erosions, bleeding, and mucosal injury. Arabica coffee fruit skin contains antioxidant substances, which are expected to have a good role as an antioxidant effect. Currently, unknown effects of alcohol and extract of Arabica coffee fruit skin administration on the damage of the small intestine of mice (Mus musculus L.). This study used an experimental design, and 25 males of mice were divided into five groups of treatment for 15 days. After the treatment, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. The Data analyzed used One Way ANOVA and Duncan's tests. Histopathological observations showed a dose of 250 mg/kg BW experienced the most significant improvement damage of the small intestine of mice compared to other treatments. Histomorphometry measurement, The dose of 250 mg/kg BB showed the high of villi 272,10 ± 13,36 µm, (P<0.05), duodenal villi crypt depth 117,33 ± 30,35 µm (P<0.05) and surface area of small intestinal villi 7,66 ± 0,55 mm2 (P<0.05) was similar with negative control. The conclusion of this study is a dose of 250 mg/kg BW treatment was able to improve the histopathology and morphometry small intestine of mice that were damaged due to consumption of ethanol 15%.
Antibacterial Activity of Coffee Arabica (Coffea arabica L.) Fruit Skin Methanol Extract On Bacteri Eschericia coli and Staphylococcus aureus Rita Maliza; Jannati Aulah; Oktira Roka Aji
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108692-0-00

Abstract

Coffee fruit skin is a waste from coffee bean processing. Arabica coffee bean extract (Coffea arabica L.) is known to contain anthocyanin compounds, polyphenols, beta-carotene, and vitamin C which have the potential as antimicrobial agents. The purpose of this study is identification and anaysis the optimal concentration of Arabica coffee peel extract which inhibits the bacteria Escherichia coli and Staphylococcus aureus. This study used an experimental method consisting of 3 extract concentration treatments, positive control, and negative control with 3 repetitions. The treatments tested were 25%, 50%, 75% coffee fruit peel, negative control (sterile distilled water) and positive control (Ampicillin 2 µg / ml). The bacterial test was carried out using the in vitro method. The parameters measured were the amount of inhibition zone (mm) formed on the Mannitol Salt Agar (MSA) and Mac Conkey media. Analysis of research data using the One-way ANOVA test and Duncan test at α = 0.05. The results showed that the optimum concentration of the extract against Staphylococcus aureus was 50%, while the optimum concentration of Escherichia coli was 25%. The results of this study indicate that the extract can be an antibacterial that can inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria optimally.  
Co-Authors . Mansyurdin Adam Raihan Priambada Adi Wira Septama Adilla, Ardis Trianita Adnan, Adnan Afdhal Raihan Afdhal Raihan Afif , Muhamad Aldi Tamara Rahman Alfiatul Laila Alimuddin Tofrizal Allimuddin Tofrizal Amelia Roza, Amelia Angga Illahi Kurnia Illahi Angga Kurnia Illahi Anisa Nova Puspitaningrum Aprilia Cassa Nova Aprilia, Kharisma Annisa Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Aszareta, Muhammad Andoni Athika Darumas Putri Aulia Rahmi Aulia, Annisa Ayu Rosemeilia Dewi Aziz, M. Abdul Azizah Amatu zikrah Azizah Amatu Zikrah Azzahra, Layla Bayu Tri Murti Bramadi Arya Cahyani, Dini Chairunnisa Azzahra Azzahra H Cheung, Rocky Dahelmi Dahelmi Diana Fadhilah Diani Fatmawati Dyah Aryani Perwitasari Efa Refiani Efrizal Efrizal Eli Ratni Elisa, Tasya Putri Pratama Elni Fatimah Era Refiani Esa Savitri Eti Yerizel Fadhilah, Diana Fadillah Fadillah Fadillah Fajri Hidayat Febriofca Galih Yatalaththov Firmansyah, Tio Gina Noor Djalilah Haafizah Dania Haafizah Dania Haafizah Dania Hamdani, Afif Haris Setiawan Harry Nugroho Eko Surniyantoro Henny Herwina Hevi Horiza Hulrahma, Mifta Husna Fitri Husna Fitri Husna Fitri Husna Fitri Hylda Ibrahim, Muhammad Hamdi Ika Wahyuni Ikrimah Nisa Utami Ilham, Kurniadi Illahi, Angga Illahi Kurnia Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Inggita Utami Irwan , Reziq Marchellino Ivan Petrovsky Jabang Nurdin Jannati Aulah Janra, Muhammad Nazri Jasmi Jefrial Santoso Kania Agustina Kiky Martha Ariesaka Kurniadi Ilham Lalu Muhammad Irham Lestari, Mardiana Puji Liana Fairuzannah Mahdiana Listiatie Budi Utami Lutfi Sukma Pratiwi Made Ary Sarasmita Mahardika, Riris Srikandi Melinda Widianingrum Melinda Widianingrum Melinda Widyaningrum Melodia Rezadhini Miftahul Ilmi Moh. Mirza Nuryady Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Idris Muhammad Idris Muhammad Ilham Hisbullah Muhammad Nazri Janra Nabilah Adzra Fahlevi Nabilah Rahmachila Azura Nadjmir, Mikail Nanik Sulistyani Nastiti Rahajeng Nelvi, Dennisa Arra Ni Made Ary Sarasmita Nofrita Nofrita Nofrita Nofrita Nopiyanti, Tita Nur Aida Nur Rahmah Hidayati Nurul Annisa Nurwijayanti Oktira Roka Aji Petrovsky, Ivan Pradika, Jaka Pratama Elisa, Tasya Putri Pratama, Kharisma Pratama, Raihan Anugrah Puspitaningrum, Anisa Nova Puti Khairunnajwa Amar Putra Santoso Putri Lestari Putri, Fadilatul Rahmi Putri, Syntia Mai Raihan, Afdhal Ramadanti, Dhea Rarastoeti Pratiwi Resti Rahayu Rezadhini, Melodia Reziq Marchellino Irwan Ridho, Muhammad Syifa'ur Rifai, Fauziah Novita Putri Rihadatul Aisyah Rilwan Efendi Rismayani, Dessy Rizma Nurul Akhlas Robby Jannatan Rocky Cheung Rocky Cheung Saraswati, Utami Sinurat, Arthauly Yopita Siti Rokhimah Sumaryati Syukur Sutri Wulansari Safril Syaiful Adam Maulana Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tasya Putri Pratama Elisa Tio Firmansyah Tofrizal Tofrizal , Allimuddin Tofrizal, Alimuddin Tur Rahardjo Utami Saraswati Wardatul Aini Wilson Novarino Wirawan Adikusuma Yanti Lusiyanti Yanti, Windi Nofri Yeni Alfiana Ratnasari Yudha Rizky Nuari Yustia Tuti Zaini Zaini Zikrah, Azizah Amatu Zozy Aneloi Noli Zozy Aneloi Noni Zuhir, Elvina Zulfikri, Elzam Naufal