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Potensi Fraksi Etil Asetat Ekstrak Daun Gambir (Uncaria gambir Roxb.) sebagai Antihiperlipidemia Yunarto, Nanang; Elya, Berna; Konadi, Laurentia
Jurnal Kefarmasian Indonesia VOLUME 5, NOMOR 1, FEBRUARI 2015
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v5i1.2959

Abstract

Hyperlipidemia is the main risk factor for atherosclerosis and coronary heart disease. Ethyl acetate fraction of gambier leaves extract (Uncaria gambir Roxb.) contains catechin secondary metabolites which have potency to be used as antihyperlipidemic. This study aimed to examine the antihyperlipidemic effect of ethyl acetate fraction of gambier leaves extract in vivo. Thirty six males of Sprague Dawley strain, 2,5 months old, were randomly divided into six groups: normal group, negative group (distilled water), positive group (simvastatin 2 mg/200 g bw), dose I (5 mg/200 g bw fraction), II (10 mg/200 g bw fraction) and III (20 mg/200 g bw fraction) groups. Rats were induced with high cholesterol and saturated fat feeds for 28 days, except in the normal group. Furthermore, rats were given the treatment for 28 days. The results showed when compared than negative control, dose III reduced of total cholesterol, triglycerides, LDL levels and increased HDL level (p
Tingkat Kejadian Hipoglikemia pada Pasien Diabetes Melitus Tipe 2 Peserta JKN dan Non-JKN pada Masa Pandemi COVID-19 di Kalimantan Tengah Putu Rika Veryanti; Ratu Ayu Dewi Sartika; Rani Sauriasari; Berna Elya; Rara Merinda Puspitasari
JURNAL ILMU KEFARMASIAN INDONESIA Vol 21 No 2 (2023): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v21i2.1477

Abstract

Community activity restrictions during the COVID-19 pandemic in Indonesia impact the quantity and quality of health services for type 2 diabetes (T2DM). This limitation could increase the risk of hypoglycemia in type 2 diabetes patients. The study aimed to compare the incidence rate of hypoglycemia between national (NHIP) and non-national health insurance participants (N-NHIP) with T2DM during the COVID-19 pandemic in Indonesia. The study used a cross-sectional design and was conducted at a government hospital in Central Borneo. Data was collected by consecutive sampling from September to November 2021. Sixty-two participants were divided into two groups (NHIP and N-NHIP groups). Each group consisted of 31 participants. They were interviewed regarding their experience with hypoglycemia in the last three months. The incidence rate of hypoglycemia in N-NHIP was higher than in NHIP (93.55% vs. 87.10%; p>0.05). Hypoglycemia is mostly presented in patients using a combination of short-acting and long-acting insulin. The incidence rate of hypoglycemia in T2DM during the COVID-19 pandemic in Central Borneo was relatively high. In future studies, it is necessary to analyse the factors that significantly affect the incidence of hypoglycemia in the population of T2DM patients in Indonesia.
Tyrosinase Inhibitory Activity of The Extracts from Garcinia lateriflora Blume var. Javanica Boerl, Garcinia fruticosa Lauterb, and Garcinia xanthochymus Neneng Siti Silfi Ambarwati; Berna Elya
Journal of Pharmaceuticals and Natural Sciences Vol. 1 No. 1 (2024): J. Pharm. Nat. Sci.
Publisher : B-CRETA Publisher (CV. Borneo Citra Kreatama)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/drty6950

Abstract

Tyrosinase overexpression in humans increases melanin production in the skin, which may have hyperpigmentation-causing effects. In this study, various extracts from the Garcinia lateriflora Blume var. Javanica Boerl, Garcinia fruticosa Lauterb, and Garcinia xanthochymus plants were tested for their ability to inhibit the tyrosinase enzyme. The results showed that the G. fruticosa Lauterb stem bark hexane extract had the highest activity followed succesively by the G. lateriflora Blume var javanica Boerl leaf methanol extract, G xanthochymus mesocarp hexane extract, G. xanthochymus mesocarp methanol extract. The results show that the G. fruticosa Lauterb stem bark hexane extract has the highest tyrosinase inhibitory activity (45.61 ± 1.58%) among the four extracts and the tyrosinase inhibitory activity of kojic acid (65.07 0.86%).
Pemberdayaan Masyarakat Desa Sukajaya Sumedang Selatan dalam Produksi Minuman Kesehatan dan Bumbu Rempah berbasis Teknologi Farmasi: Empowering the Sukajaya Village Community South Sumedang in the Production of Health Drinks and Spices based on Pharmaceutical Technology Berna Elya; Roshamur Cahya Forestrania; Ratika Rahmasari; Donna Maretta Ariestanti; Syamsu Nur
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 2 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i2.5888

Abstract

As a result of observations and joint discussions with the Village Community, information was obtained that there are agricultural products in the form of spices with high annual production quantities. However, the selling value of these spices still needs to be improved. Technology has not touched them and has yet to be managed optimally. Therefore, the team empowers the community to process herbs into food products, namely health drinks and spices based on pharmaceutical technology. This activity aims to improve community welfare and mutual elements of human resources in the region. This activity was attended by 28 community members who are Partners and Village Officials. Social empowerment of the society involved in the downstream activities of agricultural products in Sukajaya Village will be carried out through socialization stages, FGD with society and village officials, establishing a micro business plan for downstream health drink products and spices building production facilities, and assistance. This activity produces products in spices, health drinks, and herbal candy. At least 80-90% of the community understands each stage of the activity so that it can become a provision for self-development. The output of this activity is in the form of innovative products, such as health drinks and spice products, which can increase partner empowerment.
Phytochemical Constituents and Evaluation of Lipoxygenase Activity of Tetracera macrophylla Twigs Wall.ex Hook.f. & Thoms Ladeska, Vera; Elya, Berna; Hanafi, Muhammad; Kusmardi, Kusmardi
JSFK (Jurnal Sains Farmasi & Klinis) Vol 11 No 1 (2024): J Sains Farm Klin 11(1), April 2024
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.11.1.1-6.2024

Abstract

Lipoxygenase is an enzyme that catalyzes the formation of leukotriene, which is involved in various diseases  such as asthma, osteoporosis and atherosclerosis. Inhibition of this enzyme can overcome inflammatory diseases. Tetracera  macrophylla Wall.ex Hook. f.& Thoms plant has shown various biological activities, especially as an anti-inflammatory. This study  aims to analyze phytochemical constituents and evaluate the lipoxygenase activity of Tetracera macrophylla twig extract. The  sample was gradually macerated using n-hexane, ethyl acetate, and methanol. Phytochemical analysis of the extract was carried  out using the LC-MS/MS method. Lipoxygenase assay was performed in vitro using RAW 264.7 macrophage cells induced with  lipopolysaccharide using spectrofluorometry. As a comparison, zileuton, which has been established as a 5-LOX inhibitor and  quercetin, which has the potential as a 5-LOX inhibitor. The results showed that methanol extract of Tetracera macrophylla twigs (RM3) had lower specific LOX activity (0.350 mU/mL) than standard zileuton (7.927 mU/mL) and quercetin (12.837 mU/mL).  Decreased LOX activity can lessen the generation of leukotrienes, which cause inflammation. The study showed that methanol extract of Tetracera macrophylla twigs contains active compounds that can be developed as lipoxygenase inhibitors
Antibacterial effects of Andrographis paniculata extract, Curcuma domestica extract, chloramphenicol and their combinations on the growth of Salmonella typhi bacteria Rahmadi, Agus; Elya, Berna; Suryadi, Herman; Prasetio, Nisrina Putri; Kamaruzzaman, Mohd Amir
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1677

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Typhoid fever caused by Salmonella typhi remains a serious health threat. Although standard treatment with antibiotics such as chloramphenicol has helped reduce mortality rates, bacterial resistance to this antibiotic is increasing. New treatment approaches are urgently needed, including combining antibiotics with natural compounds from medicinal plants, such as Andrographis paniculata and Curcuma domestica. This study aimed to compare the antibacterial effects of A. paniculata extract, C. domestica extract, chloramphenicol, and their combinations on the growth of S. typhi. This in vitro experimental study used the disc diffusion method to evaluate antibacterial activity. Antibacterial activity tests were performed against S. typhi using discs soaked in 70% ethanol extract solutions of A. paniculata and C. domestica, chloramphenicol, and their combinations. Inhibition zones were measured after incubation for 24 hours at 37°C. Chloramphenicol showed the strongest antibacterial activity with a mean inhibition zone of 28.33 ± 0.58 mm. Single extracts of A. paniculata and C. domestica had relatively weak antibacterial activity (inhibition zones of 9.67 ± 1.15 mm and 9.83 ± 0.29 mm) and there was no significant difference between them (p>0.05). Combinations of extracts with chloramphenicol showed increased antibacterial activity compared to single extracts (inhibition zones of 23.17 ± 1.26 mm for A. paniculata + chloramphenicol and 21.00 ± 2.65 mm for C. domestica + chloramphenicol) and there were significant differences between combinations and single extracts (p<0.05), but still lower than single chloramphenicol and statistically significant (p<0.05). Although combining medicinal plant extracts with chloramphenicol increased antibacterial activity compared to single extracts, it did not exceed single chloramphenicol.
Ethanol extract from Ziziphus nummularia stem inhibits MCF-7 breast cancer cell proliferation through TP53 regulating kinase (TP53RK)-mediated p53 activation: In silico and genes expression investigations Elya, Berna; Rosmalena, Rosmalena; Fajrin, Ajeng M.; Tedjo, Aryo; Ramadanti, Nur A.; Azizah, Norma N.; Hashim, Najihah BM.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1382

Abstract

The p53 signaling pathway plays a critical role in regulating the cell cycle, apoptosis, and senescence, making it a key target in cancer research. The aim of this study was to investigate the effects of an ethanol extract from the stem of Ziziphus nummularia on the proliferation and expression of genes involved in the p53 pathway in MCF-7 breast cancer cells. To achieve this, real-time quantitative PCR (RT-qPCR) was used to evaluate the mRNA expression of downstream genes linked to cell cycle and senescence, including CycE or CCNE1, RBL1, and E2F1. Molecular docking simulations using Molegro Virtual Docker (MVD) were also performed to assess the potential inhibitory activity of metabolite compounds from Z. nummularia stem against p53-regulating kinase (TP53RK). The results showed that the IC50 value of Z. nummularia stem ethanol extract against MCF-7 cells was 38.27±0.72 µg/mL. The results also revealed a reduction in the expression of downstream genes linked to cell senescence and the cell cycle: CycE or CCNE1 (p=0.011), RBL1 (p=0.008), and E2F1 (p=0.005), which was observed through RT-qPCR analysis of mRNA expression. This fact indicated that the inhibitory effects on proliferation by the ethanol extract of Z. nummularia stem might occur via pathways associated with cell senescence and cell cycle arrest.  Molecular docking results of metabolite compounds from Z. nummularia stem suggested that squalene (Rerank score -112.70 kJ/mol), and nummularine B (Rerank score -110.68 kJ/mol) had potential as TP53RK inhibitors. These Rerank scores were smaller compared to the Rerank score of adenyl-imidodiphosphate (AMP-PNP), which was the native ligand of TP53RK, as confirmed by molecular dynamics analysis. These in silico results were confirmed by the decrease in p21 (CDKN1A) mRNA expression. In conclusion, the anti-proliferative effects of the ethanol extract from Z. nummularia stem on breast cancer cells occurred by affecting cell cycle-related genes and inhibiting apoptosis protection mediated by overexpression of p21 (CDKN1A) through p53 activity.
Phytochemical analysis and antibacterial activity of Staurogyne sp. in fish disease bacteria Nugraha, Media Fitri Isma; Novita, Hessy; Rajamuddin, Muh Alias; Yunita, Rossa; Enggarini, Wening; Reflinur, Reflinur; Maharani, Fasya Hadaina; Elya, Berna
Torani Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i2.7057

Abstract

This study was aimed to identify the potential use of active compounds extracted from Staurogyne sp as an antibacterial agent to control disease-causing bacteria in fish. Staurogyne sp, an aquatic plant collected from Bantimurung, South Sulawesi were subjected to allelochemical compound. Plant extracts were tested to five bacteria including four types of bacterial pathogen, such as Aeromonas hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, and Flavobacterium columnare, and one bacterial sensing quoroum, Chromobacterium violaceum. Based on phytochemical analysis, Staurogyne’s extracts derived from both stem and root contained flavonoids, phenols, and antioxidants compounds with the concentration of 0.018 mgQE/g, 0.3471 mgGAE/g, and  1004,391 IC50 µg/mL, respectively. In addition, Staurogyne plant extracts derived from both their stem and root has also produced both saponins and glycosides compounds. Plant extracts derived from Staurogyne’s leaves revealed three major chemical compounds such as flavonoids (0.77 mgQE/g), phenol (0.0629 mgGAE/g), and glycosides (+). However, no antioxidants and saponin compounds were detected.  Applying plant extracts as an antibacterial on five disease-causing bacteria causing diseases in aquaculture, such as Aeromonas Hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, Flavobacterium columnare, and Chromobacterium violaceum bacterials revealed that at concentration of 0.1 g the Staurogyne sp extracts did not influence all the bacterials growth. However, by application of plant extract at concentration of 0.2 g on the growth media,three out of 5 tested bacterials (Aeromonas hydrophilla, Edwardsiella ictaluri, and Streptococcus agalactiae) showed intermediate inhibition responses on bacterial growth. The growth of remaining two bacterials pathogen, Flavobacterium columnare and Chromobacterium violaceum at 0.2 g plant extract of Staurogyne sp was not affected. This study revealed that the use of active compound derived from Staurogyne sp would be potential to be used in inhibiting disease-causing bacterial in fish in future. However, optimum concentration of the plant extracts, in particular on the inhibition of the growth of disease-causing bacteria in fish is still needed to adjust.Key words: Antibacterial, aquatic plant, Staurogyne sp, fish disease
Analisis Mikroskopis, Standarisasi dan Profil Kandungan Kimia secara LC-ESI-MS dari Ekstrak Etil Asetat Curculigo latifolia Sari, Rifta; Rahmasari, Ratika; Nur, Syamsu; Elya, Berna
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 1 (2025): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i1.736

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Curculigo latifolia merupakan tanaman obat yang memiliki potensi farmakologis signifikan. Penelitian ini bertujuan untuk mengidentifikasi karakteristik mikroskopis, melakukan standarisasi simplisia, dan menganalisis profil metabolit ekstrak etil asetat daun C. latifolia ini menggunakan LC-ESI-MS. Penelitian ini dilakukan dengan tahapan pembuatan simplisia dari masing-masing bagian tanaman C. latifolia yaitu akar, pelepah dan daun dan selanjutnya dilakukan analisis mikroskopis dengan menggunakan mikroskop binokule dengan pembesaran 400x. Simplisia bagian daun diekstrak secara bertingkat yang dimulai dengan n-heksan dan dilanjutkan etil asetat dan etanol 70%. Ekstrak etil asetat bagian daun distandarisasi dan diidentifikasi metabolit sekundernya dengan metode LC-MS/MS. Hasil Pengamatan mikroskopis menunjukkan adanya struktur trikoma, kristal kalsium oksalat dalam berbagai bentuk, dan berkas pembuluh pada daun, pelapah, dan akar, yang mendukung fungsi fisiologis tanaman. Standarisasi simplisia mengungkapkan bahwa kadar air (6,32±0,66%), kadar abu total (0,32±0,04%), bobot jenis (1,01±0,00), dan susut pengeringan (6,71±0,52%) telah memenuhi persyaratan Farmakope Herbal Indonesia. Analisis LC-ESI-MS mengidentifikasi sepuluh senyawa bioaktif, yaitu valeroidine, afzelin, (-)-caryophyllene oxide, triptolide, epi-tulipinolide, testosterone acetate, 11-hydroxyetiocholanolone, 3-deoxyestradiol, 13-hydroxy-alpha-tocopherol, dan 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylic acid. Penelitian ini memberikan landasan ilmiah untuk pengembangan C. latifolia sebagai bahan obat herbal modern yang berkualitas serta menjadi model bagi studi komprehensif terhadap tumbuhan obat lainnya.
EFFECTIVENESS OF AMLODIPINE WITH CONCOMITANT HERBAL THERAPY (BAY LEAVES, CELERY, CUCUMBER) IN HYPERTENSIVE PATIENTS IN SURAKARTA Mahmuda, Nur Amalia; Andrajati, Retnosari; Elya, Berna
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 17 No 2 (2025): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v17i2.2939

Abstract

Hypertension is a chronic disease that requires long-term management. The use of medicinal plants as complementary therapy is increasingly recognized, although scientific evidence remains limited. This study aimed to evaluate the effectiveness of amlodipine with concomitant herbal therapy (bay leaves, celery, and/or cucumber) compared with amlodipine monotherapy in hypertensive patients. A retrospective cohort design was employed using medical record data and patient questionnaires from three community health centers in Surakarta. Treatment effectiveness was measured by the achievement of the target blood pressure over three months. Data were analyzed using bivariate tests and multivariate logistic regression. The results showed that clinical effectiveness was higher in the amlodipine group with concomitant herbal therapy (bay leaves, celery, and/or cucumber) at 78.4% compared to amlodipine monotherapy at 68.8%, although the difference was not statistically significant (p=0.160). Factors significantly associated with treatment effectiveness were medication adherence (p<0.001), baseline systolic blood pressure (p<0,001), and educational level (p=0,047). Concomitant herbal therapy with amlodipine showed potential blood pressure–lowering effects, though not statistically significant. Prospective studies or clinical trials are warranted to further assess the benefits and safety of this therapeutic approach.
Co-Authors . Azizahwati . Azizahwati . Farida . Frengki . Katrin . Katrin . Nofiantini Ali Muhammad Shodiq Amarila Malik Anastasia Bangun Anastasia Bangun, Anastasia Arifayu Addiena Kurniatri Arifianti, Ayun Erwina Aryo Tedjo Atiek Soemiati Atiek Soemiati Atiek Soemiati Atriyon Julzarika Atriyon Julzarika Azizah, Norma N. Azizahwati Azizahwati Azminah Azminah Basah, Katrin Chairunissa, Ananda Hanny Charissa, Meiliana Daan Khambri Dadang Kusmana Dadang Kusmana Deddi Prima Donna Maretta Ariestanti Donna Maretta Ariestanti Effionora Anwar Elis Apriyanti Elis Apriyanti, Elis Emiyanah . Emiyanah, Emiyanah Fadlina Chany Saputri Fajriati, Annisa Fajrin, Ajeng M. Farida Farida Farida Ibrahim Fasya Hadaina Maharani Fatma Sri Wahyuni Febriani, Amelia Febrina Amelia Saputri Forestrania, Roshamur Cahyan Frengki Frengki Fulka Nurzaman Hanita Omar Hashim, Najihah BM. Hayun Hayun, Hayun Henny Vanda Herman Suryadi Hessy Novita Hindun Wilda Risni Iis Irawatty Dewi Ika Maruya Kusuma Ika Mustika Imam Syafi&#039;i Irianti, Marina Ika Islamudin Ahmad Jamilah Abbas Jamilah Abbas Jantan, Ibrahim Joshita Djajadisastra Joshita Djajadisastra Joshita Djajadisastra, Joshita Juheini Amin Juheini Amin Juheini Amin Julian, Muhamad Iqbal Kamaruzzaman, Mohd Amir Kartika Citra Dewi Permata Sari Katrin Basah Katrin Katrin Krinalawaty, Nevy Kusmardi Kusmardi Ladeska, Vera Liza Ariantina Maharani, Fasya Hadaina Mahdi Jufri Mahmuda, Nur Amalia Makau, Juliann Nzembi Marcha Putri, Nada MARIA BINTANG Martha, Septiani Media Fitri Isma Nugraha Meiliana Charissa Mia Permawati Mihardja, Laurentia K. Muh Alias L Rajamuddin Muhamad Iqbal Julian Muhammad Hanafi Muhammad Hanafi Muhammad Hanafi MUHAMMAD HANAFI Muhammad Muhammad Nanang Yunarto Nanang Yunarto Neneng Siti Silfi Ambarwati Nevy Krinalawaty Nisa Maria Nofiantini Nofiantini Nofiantini Nofiantini, Nofiantini Noni Zakiah Novi Fajar Utami Novi Sulistyaningrum Novita, Hessy Nugraha, Media Fitri Isma Nurzaman, Fulka Prasetio, Nisrina Putri Rahmadi, Agus Rahmasari, Ratika Rajamuddin, Muh Alias Rajamuddin, Muh Alias L Ramadanti, Nur A. Rani Sauriasari Rara Merinda Puspitasari Ratika Rahmasari Ratu Ayu Dewi Sartika Reflinur Reflinur REFLINUR REFLINUR Regita Putri, Jasmine Retnosari Andrajati Rieka Widihasputri Rieka Widihasputri, Rieka Robiatul Adawiyah Rohmat, Siti Saudah Roshamur Cahya Forestrania Roshamur Cahyan Forestrania Roshamur Cahyan Forestrania Rosmalena, Rosmalena Rossa Yunita ROSSA YUNITA Santy Rahmawati Sari, Atika Mutiara Sari, Rifta Sintiami Ramadhani Siti Khadijah Soleh Kosela Soleh Kosela Syafi'i, Imam Syamsu Nur Syamsu Nur, Syamsu Taufiq Indra Rukmana Veryanti, Putu Rika Wening Enggarini Wening Enggarini Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yesi Desmiaty, Yesi Yuditya Artha