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PERTUMBUHAN PLANLET KRISAN (Dendranthema grandiflora Tzvelev) KULTIVAR PINK FIJI SETELAH PENAMBAHAN EKSTRAK TAUGE (Vigna radiata L.) PADA MEDIUM MURASHIGE DANSKOOG (MS) SECARA IN VITRO. Nalindri Impitasari; Endang Nurcahyani; Tundjung Tripeni Handayani; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 5 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v5i2.50

Abstract

Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the important ornamental plants in Indonesia and has high economic value . This plant is known as a producer of flowers with attractive shapes and colors . Seeing the magnitude of community interest and the potential utilization of chrysanthemum , causing this plant more and more developed and cultivated . This study aims to determine the concentration of optimum mungbean sprouts extract on the growth of chrysanthemum explants in vita . The addition of mungbean sprouts extracts (Vigna radiata L .) from concentration of 0% v/v , 2% v/v , 4% v/v , 6% v/v and 8% v/v on Murashige and Skoog(MS ) medium to growth eksplan Chrysanthemum (Dendranthema grandiflora Tzvelev ) Pink Fiji cultivars have been carried out in the tissue culture laboratory of Faculty of Mathematics and Natural Sciences , University of Lampung from November to December 2017 . This study used Completely Randomized Design (RAL ) 1 factor with 5 replications . Analysis of BNT variety and test is done at 5% level . The results showed that the extract from mungbean sprouts (Vigna radiata L . ) had no effect on plantlet height , number of shoot and number of chrysanthemum (Dendranthema grandiflora Tzvelev) plantlet leaves. The addition of mungbean spourts extracts on Murashige and skoog (MS) medium show 100% live plantlet.
ANALISIS LIGNIN DAN STRUKTUR ANATOMI PLANLET TOMAT (Lycopersicum esculentum MILL) HASIL SELEKSI ASAM SALISILAT SECARA IN VITRO Endang Nurcahyani; Lindawati Lindawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 2 No. 2 (2014)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v2i2.115

Abstract

Kajian tentang seleksi planlet tomat dengan asam salisilat konsentrasi 0, 15, 30, 45, dan 60 ppm secara in vitro terhadap struktur anatomi dan lignifikasi, telah dilaksanakan di Laboratorium Kultur Jaringan, Jurusan Biologi, Fakultas MIPA,Universitas Lampung. Penelitian ini dilakukan dari bulan Juni sampai Agustus 2014. Data yang diperoleh selama seleksi dengan asam salisilat berupa data kualitatif dan data kuantitatif. Data kualitatif disajikan dalam bentuk deskriptif komparatif dan di dukung foto. Dalam penelitian ini digunakan rancangan acak lengkap dengan 6 ulangan. Analisis ragam dan uji BNT dilakukan pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa ketebalan lignin pada xilem batang planlet tomat yang diimbas asam salisilat pada konsentrasi 15, 30, dan 60 ppm menunjukkan ketebalan yang lebih besar dibandingkan kontrol, sedangkan pada konsentrasi 45 ppm ketebalannya lebih kecil dibandingkan kontrol. Struktur anatomi batang planlet tomat yang diimbas asam salisilat dibandingkan dengan kontrol terdapat perbedaan pada bagian epidermis, jari-jari empulur, dan kambium. Pada epidermis, jari-jari empulur, dan kambium batang yang diimbas asam salisilat mengalami lignifikasi.
Analisis Klorofil Planlet (Spathoglottis plicata) Bl Plantlet Terhadap (Fusarium oxysporum) Gardis Andari; Endang Nurcahyani; Adrianus Adrianus
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1207.172 KB)

Abstract

Orchids are ornamental plants that are in great demand by Indonesians, their prices are also very high. There are disturbances that occur in the growth of orchids, namely root rot and leaf spot. Fusarium wilt disease caused by Fusarium oxysporum is an important disease because it becomes an obstacle in the quality and production of orchids. The death rate of orchids in the United States caused by Fusarium oxysporum reaches more than 50% of the total number of orchids and this disease is difficult to control using fungicides. One of the efforts to control fusarium wilt disease safely, effectively, efficiently, and without negative impacts is by using high yielding varieties through tissue culture. Tissue culture applications using fusaric acid (AF) are widely used for in vitro selection of many plants. Plants infected with impacting materials will provide a resistance response (Induced resistance) and function to maintain plant survival, especially in warding off the attack of dangerous pathogens. The aim of this study is the specific expression character ofplantlets Spathoglottis plicata resulting from induced resistance to F. oxysporum based on total chlorophyll content, chlorophyll a chlorophyll b. The research method used tissue culture. The overall results of the study on chlorophyll a, clrophil b and total chlorophyll increased and were significantly different. In the control, the chlorophyll a content was 1.722 ± 1.1309E-02, chlorophyll b was 0.585 ± 2.5537E-02 and total chlorophyll was 2.309 ± 2.7503E-02. The chlorophyll a content in the 10 ppm treatment was 2.157 ± 4.7393E-02, the chlorophyll b was 1.470 ± 1.3237E-02, and the total chlorophyll was 3.625 ± 1.0504E-01. The chlorophyll a, b and total content increased in the 20 ppm treatment, namely chlorophyll a 2.834 ± 5.8198E-03, chlorophyll b 2.582 ± 1.8300E-03, total chlorophyll 5.413 ± 8.1599E-03. Chlorophyll a, b and total chlorophyll increased in 30 ppm treatment, namely chlorophyll a 3.297 ± 2.9527E-04, chlorophyll b 3,966 ± 9.1400E-03 and total chlorophyll of 7,258 ± 6.1367E-03. In the 40 ppm treatment, the content of chlorophyll a, b and total chlorophyll increased significantly, namely in chlorophyll a 3.957 ± 1.8898E-02, chlorophyll b 5.642 ± 3.6749E-01 and total chlorophyll of 9.592 ± 2.2269E-01 so it can be concluded that Chlorophyll a, b, and total chlorophyll content in ground orchid plantlet leaves that were resistant to Fusarium oxysporum increased compared to controls with higher concentrations of fusaric acid given ABSTRAK Anggrek merupakan tanaman hias yang banyak diminati oleh masyarakat indonesia, harganya juga sangat tinggi. Terdapat gangguan yang terjadi pada pertumbuhan tanaman anggrek, yaitu penyakit busuk akar dan bercak daun. Penyakit layu fusarium disebabkan Fusarium oxysporum merupakan penyakit penting karena menjadi salah satu kendala dalam kualitas dan produksi tanaman anggrek. Tingkat kematian tanaman anggrek di Amerika Serikat yang disebabkan oleh Fusarium oxysporum mencapai lebih dari 50% dari jumlah tanaman anggrek dan penyakit ini sulit dikendalikan dengan menggunakan fungisida. Salah satu usaha untuk mengendalikan penyakit layu fusarium dengan aman, efektif, efisien, tidak menimbulkan dampak negatif yaitu dengan menggunakan varietas unggul melalui kultur jaringan. Aplikasi kultur jaringan menggunakan Asam fusarat (AF) banyak digunakan untuk sleksi in vitro pada banyak tanaman. Tanaman yang diinfeksi bahan pengimbas akan memberikan respon ketahanan (Induced resistance) dan berfungsi untuk mempertahankan kelangsungan hidup tanaman, khususnya dalam menangkal serangan patogen yang berbahaya  Tujuan penelitian ini adalah Karakter ekspresi spesifik planlet Spathoglottis plicata hasil induce resistance terhadap F. oxysporum berdasarkan kandungan klorofil total, klorofil a klorofil b. Metode penelitian menggunakan kultur jaringan. Hasil penelitian secara keseluruhan pada klorofil a, klrofil b dan klorofil total mengalami peningkatan dan berbeda nyata. Pada kontrol  kandungan klorofil a yaitu 1,722 ± 1.1309E-02, klorofil b 0,585 ± 2.5537E-02  dan klorofil total 2,309 ± 2.7503E-02. Kandungan klorofil a pada perlakuan10 ppm yaitu 2,157 ± 4.7393E-02 , pada klorofil b 1,470 ± 1.3237E-02, dan pada klorofil total yaitu 3,625 ± 1.0504E-01. Kandungan klorofil a, b dan total mengalami peningkatan pada perlakuan 20 ppm yaitu klorofil a 2,834 ± 5.8198E-03 , klorofil b 2,582 ± 1.8300E-03 , klorofil total 5,413 ± 8.1599E-03. Pada klorofil a, b dan klorofil total mengalami peningkatan pada perlakuan 30 ppm yaitu pada klorofil a 3,297 ± 2.9527E-04, klorofil b 3,966 ± 9.1400E-03 dan klorofil total yaitu 7,258 ± 6.1367E-03. Pada perlakuan 40 ppm, kandungan klorofil a,b dan klorofil total mengalami peningkatan yang berbeda nyata yaitu pada klorofil a 3,957 ± 1.8898E-02 , klorofil b 5,642 ± 3.6749E-01dan klorofil total yaitu 9,592 ± 2.2269E-01  sehingga dapat disimpulkan bahwa kandungan klorofil a, b, dan klorofil total pada daun planlet anggrek tanah yang tahan terhadap Fusarium oxysporum mengalami peningkatan dibandingkan kontrol dengan semakin tinggi konsentrasi asam fusarat yang diberikanKey words: in vitro, klorofil, Spathoglottis plicata
GEN KETAHANAN PENYAKIT PADA FAMILIA ORCHIDACEAE Risma Rasmani; Endang Nurcahyani; Sri Wahyuningsih; Sumardi Sumardi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.10570

Abstract

Familia Orchidacae memiliki banyak jenis spesies yang terdiri dari 25.000 spesies.  Beberapa contoh spesies dari Orchidaceae yaitu diantaranya adalah anggrek bulan, vanilli, anggrek tanah, Dendrobium dan lain-lainnya. Tanaman seperti anggrek dan vanilli terancam kepunahannya. Tanaman anggrek dan vanilli sering terserang oleh jamur patogen yaitu Fusarium oxysporum (Fo) yang dapat menimbulkan penyakit layu tanaman. Pengendalian penyakit yang tidak menimbulkan efek negatif bagi lingkungan sekitarnya yaitu menggunakan kultivar unggul yang resisten terhadap infeksi dari jamur patogen F. oxysporum atau melalui penambahan senyawa- senyawa atau melalui media lainnya. Tujuan review jurnal ini adalah untuk mengetahui senyawa dan gen apa saja yang tahan penyakit pada familia Orchidaceae. Berdasarkan hasil review dari beberapa jurnal dapat diketahui bahwa senyawa-senyawa seperti Asam Fusarat, Asam Salisilat dan PEG 6000 dapat menunjukkan korelasi positif antara ketahanan penyakit terhadap toksin. Gen-gen seperti gen KNAT1, POH 1, TCP, nptII, hpt dan LTP merupakan gen-gen yang tahan terhadap penyakit dan juga gen yang ditemukan dapat mempercepat proses pertumbuhan tanaman. Hasil review beberapa jurnal diperoleh kesimpulan bahwa senyawa yang tahan terhadap penyakit tanaman yaitu Asam Fusarat, Asam Salisilat dan PEG 6000. Gen yang tahan penyakit yaitu gen KNAT1, gen POH 1, gen TCP, gen nptII dan hpt dan gen LTP. Hasil yang didapat tidak hanya tahan terhadap penyakit, tetapi senyawa dan gen tersebut dapat meningkatkan baik pada pertumbuhan seperti daun, bunga dan batang. Kata Kunci : Gen, Ketahanan, Orchidaceae, Penyakit, dan Senyawa
PENEKANAN PERKEMBANGAN PENYAKIT BUSUK BATANG VANILI (FUSARIUM OXYSPORUM F.SP. VANILLAE) MELALUI SELEKSI ASAM FUSARAT SECARA IN VITRO Endang Nurcahyani; Issirep Sumardi; Bambang Hadisutrisno; E. Suharyanto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.046 KB) | DOI: 10.23960/j.hptt.11212-22

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The most biological constrain on Vanilla planifolia plantation recently was caused by epidemical disease that laterdecrease vanilla production. The most important disease on vanilla is foot rot disease caused by Fusarium oxysporum f. sp.vanillae. So far, the disease has not been successfully controlled although some experiments had been conducted. Onealternative method has been introduced by using a new cultivar which was resistance to Fusarium). A mutant vanilla to thefungus has been initiated by in vitro selection on medium containing fusaric acid. The aims of this research were: (1) to investigate effective concentration of fusaric acid used for in vitro selection, (2) to characterize mutants which have been set up and also to test those mutants for their resistance to the fungus. The results showed that: (1) fusaric acid at the concentration of 110 ppm effectively suppressed the disease intensity up to 25% compared to the concentration of 90 ppm and 100 ppm. In other words, 110 ppm of fusaric acid has increased the category criterion from moderate to resistant, (2) there was an increase of the total phenol content and thickness of lignin in vanilla stem, and (3) the protein profile of vanilla plantlet was different from the control. There was an initiation of a new band of about 18 kD in a mutant predicted as a protein which is responsible for vanilla resistance to Fusarium.
Aktivitas Enzim Peroksidase Daun Planlet Pisang Ketan (Musa paradisiaca L.) Hasil Pengimbasan Ketahanan terhadap Asam Salisilat secara In Vitro Imamah Muslimah; Endang Nurcahyani; Zulkifli Zulkifli
Jurnal Penelitian Pertanian Terapan Vol 17 No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.827 KB) | DOI: 10.25181/jppt.v17i2.288

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Banana plant production are decreased because it getting an attack from Fusarium oxysporum. “ketan” Banana plantlet that resistant to the Fusarium oxysporum were selected by in vitro in the solid Murashige and Skoog (MS) medium with added salicylic acid at 40 ppm, 50 ppm, 60 ppm, and 70 ppm concentration, compared with controls (0 ppm). The aim of this research is to determine the activity of peroxidase enzyme on leaves of banana ketan plantlet. The research was carried out in December 2015 to February 2016 in the Laboratory of Botani (in vitro room), Departement of Biology, Faculty of MIPA, Lampung University. This study used a completely randomized design with 5 replications. Data were analyzed with the variance (Anova) and if them different will be continued by LSD test performed at 5% significance level. Extraction of peroxidase method using Saravanan et al. (2004) with the mixture of pyrogallol and 𝐻2𝑂2. The absorbance was measured with a spectrophotometer (Shimudzu UV 800) at wavelength (λ) of 420 nm and readable from zero. The result showed that the activity of peroxidase enzyme on the leaves banana ketan plantlet has increased optimally in the salicylic acid concentration of 50 ppm compared with controls. Increased of Peroxidase enzyme activity showed that banana ketan plantlet was resistant to the salicylic acid and expected to be resistant to Fusarium oxysporum. Keywords: Salicylic acid, Peroxidase enzyme, Fusarium oxysporum, in vitro, Banana ketan
Uji Ketahanan Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Seleksi dengan Asam Salisilat Terhadap Fusarium oxysporum Secara In Vitro Ria Aulia Noviantia; Endang Nurcahyani; Martha Lulus Lande
Jurnal Penelitian Pertanian Terapan Vol 17 No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.099 KB) | DOI: 10.25181/jppt.v17i2.292

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Phalaenopsis amabilis cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum. Plantlet P.amabilis which resistant to F. Oxysporum was selected in the solid Vacin and Went (VW) medium was added with salicylic acid at concentrations of 65 ppm, 75 ppm, and 85 ppm, compared with controls (0 ppm). The goals of the research were to study and determine of: 1) The SA concentration of planlet P. amabilis selection tolerant; 2) The proper concentration of AS during in vitro selection for suppressing the Fo. The research was carried out in December 2015 to February 2016 in the Botany Laboratory (in vitro research room), Departement of Biology, Faculty of MIPA, Lampung University. The result showed that: 1) The SA tolerant concentration for plantlet selection with P. amabilis was between 65 ppm - 85 ppm. 2) The 85 ppm of SA was effective for suppressing the Fo compared to 65 ppm and 75 ppm. Keywords: Phalaenopsis amabilis, salicylic acid, Fusarium oxysporum, In vitro, Resistance.
Efektivitas Ekstrak Daging Buah Nanas (Ananas comosus L.) Dalam Penurunan Indeks Browning Dari Umbi Kentang (Solanum Tuberosum L.) Putri Wardanis; Zulkifli Zulkifli; Martha L. Lande; Endang Nurcahyani
Jurnal Penelitian Pertanian Terapan Vol 19 No 2 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i2.1568

Abstract

The purpose of this study was to determine whether the pineapple fruit extract can inhibit theprocess of browning on the potato tubers. This study used Completely Randomized Design(RAL) with 5 levels of pineapple fruit concentration of 0% v/v, 25% v/v. 50% v/v, 75% v/v,and 100% v/v and consist of 5 replications. Qualitative Parameters in this study was the colosurface of potato tubers while quantitative parameters were browning index, total soluble carbohydrate content, and dehydrogenase enzyme activity homogeneity of variance, analysisof variance, and Tukey test were conducted at 5% significant level. Correlations betweendependent and independent variables were determined by linear regression. The resultshowed that the color surface of potato tuber treated with the concentration of pineappleextract 100% v/v was less brown than control and other concentrations. Index browning ofpotato tuber treated with concentration 75% v/v and 100% v/v was significantly decreased. Concentration of pineapple extract was negative linearly correlated to browning index ofpotato tubers. The concentration of pineapple extract 100% v/v increased significantly totalsoluble carbohydrate of potato tubers. The concentration of pineapple extract was positivelinearly correlated to total soluble carbohydrate. The activity of dehydrogenase enzyme ofpotato tuber treated with concentration 100% v/v was significantly increased. Theconcentration of pineapple extract was quadratic correlated to the activity of dehydrogenaseenzyme.
Penggunaan Compost Tea Diinduksi Inokulum Fungi Lignoselulolitik Pada Media Tanam Cocopeat Terhadap Pertumbuhan Tanaman Kailan (Brassica oleracea L.). Sally Khoirunisa Sally Khoirunisa; Bambang Irawan; Rochma Agustrina; Endang Nurcahyani; Sri Wahyuningsih
Jurnal Penelitian Pertanian Terapan Vol 21 No 1 (2021)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v21i1.1731

Abstract

Compost tea is a compost extract that has many nutrients. In addition, the planting medium is also important in plant cultivation. Cocopeat is very suitable for growing media because it can store nutrients and water. The purpose of this study was to determine the effect of giving compost tea induced by lignocellulolytic fungi on cocopeat media on the growth of kailan (Brassica oleracea L.) and to determine the type of compost tea and the best media composition on the growth of kailan plants. This research was conducted from May to July 2020 in the greenhouse of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. The research was conducted factorial using a completely randomized design (CRD) consisting of 2 factors, factor I was compost tea (A) with 2 levels of treatment, namely: compost tea aeration (A1) and compost tea nonaeration (A2). The second factor is the planting medium (B) with 3 levels of treatment ratio, namely: cocopeat and soil with a ratio of 2: 1 (B1); 1: 1 (B2); 1: 2 (B3) to obtain 6 treatment units. The control was not part of the treatment but the control was included in the graph as a comparison between treatments. The treatment was repeated 4 times. The parameters observed were plant height. The measurement data obtained were tested by the ANARA test. If there are differences in treatment, continue with the Tukey's test. The results showed that compost tea aerated (ACT) gave a higher average yield compared to compost tea noneration (NACT) while the composition of the planting medium for cocopeat: soil = 1: 2 gave a higher average yield than other media compositions.
APLIKASI COMPOS TEA TERINDUKSI INOKULUM FUNGI LIGNOSELULOLITIK PADA MEDIA TANAM COCOPEAT TERHADAP PERTUMBUHAN TANAMAN BAYAM MERAH (Amaranthus tricolor L.): APLIKASI COMPOS TEA TERINDUKSI INOKULUM FUNGI LIGNOSELULOLITIK PADA MEDIA TANAM COCOPEAT TERHADAP PERTUMBUHAN TANAMAN BAYAM MERAH (Amaranthus tricolor L.) Ayu Wulan Septitasari Ayu; Bambang Irawan; Rochma Agustrina; Endang Nurcahyani; Sri Wahyuningsih
Jurnal Penelitian Pertanian Terapan Vol 21 No 1 (2021)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v21i1.1733

Abstract

Compost tea is a source of additional nutrients that are easily utilized by plants along with water absorption so that it can improve plant growth. Apart from nutrients, the planting medium also plays an important role in supporting plant growth. Cocopeat is a good growing media composition because it can bind water and soil nutrients by forming storage pores so that the nutrients in the soil do not dissolve easily. The purpose of this study was to determine the effect of giving Compost tea induced by lignocellulolytic fungal inoculum on cocopeat growing media on the growth of red spinach (Amaranthus tricolor L.) and the effect of the interaction between compost tea and the ratio of cocopeat: the best soil to increase spinach plant growth. red. This research was carried out from May to July 2020 at the greenhouse of the Integrated Field Laboratory of the Faculty of Agriculture, University of Lampung. This study used a Factorial Completely Randomized Design (CRD) with the first factor (A) is compost tea with 2 levels, namely compost tea aerated (ACT) A1 and compost tea non-aerated A2, and the second factor (B) is planting media using 3 levels, namely ( B1) cocopeat and soil (2: 1), (B2) cocopeat and soil (1: 1), (B3) cocopeat and soil (1: 2) with control was only used as a comparison of the average value. Each treatment unit was carried out. as much as 4 repetitions. The parameters observed included the number of leaves. Analysis of variance at the significance level of α 5%. Then to see the difference between treatments with Tukey's test at the significant level of α 5%. The results of this study indicate that ACT and cocopeat growing media: soil = 1: 2, as well as the interaction of the two produces the highest value compared to other treatments.
Co-Authors Adrianus Adrianus Agung Kurniawan Agung Kurniawan Agustin, Resya Tamara Agustrina, Rochmah Alfian Bagus Setiawan Amini, Nur Aisyah Amirah Afifah Melta Anis Ashari Annindya Rahma Apherta, Nurshella Asrini Puspitasari Ayu Wulan Septitasari Ayu Azahra Putri Najla Azizah, Enur Bambang Hadisutrisno Bambang Irawan Bambang Irawan Damayanti, Elok Della Apriyanti Desti Deria Rahmadani Desti Syahfitri Dewiyatno, Rezza Kusumma Dian Alfiah Dian Pratiwi Dwi Septiani Dwi Septiani E. Suharyanto Eko Nastiti Eko Pramono Elsi Diana Emantis Rosa Emantis Rosa Endang Linirin Eti Ernawiati Eti Ernawiati Eti Ernawiati Farisi, Salman Fesya Salma Putri Fithria Mumtazah, Dzul Gardis Andari Hardoko Insan Qudus Hardoko Insan Qudus Hendri Busman Hendri Busman Heni Pujiastuti Herlina Putri Prastiwi Ika Listiana Imamah Muslimah Imamah Muslimah, Imamah Indah Stellawati Insan Qudus, Hardoko Intan Okta Nabilla Intan Okta Nabilla Issirep Sumardi Khairunnisa Rizqika A.P Kinasih Cahyono Lili Chrisnawati Lili Chrisnawati Lili Chrisnawati Lili Chrisnawati, Lili Linda Kurnia Dewi Linda Septiani Lindawati Lindawati Lucy Adi Tama M Kanedi Maemonah, Maemonah Mahfut Martha L. Lande Martha L. Lande Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande, Martha Lulus Maryani, Rina Ma’ania Zalzabila Meilyana Santa Maria Meilyana Santa Maria Moza Fierda Atiek Muhamad Rizkci Sazilly Nabela H.N. Harfiani Nadia Fakhriyati Arfa Nadya Rosyalina Nalindri Impitasari Ni Made Nada Elsika Nuning Nurcahyani Nur Aisyah Amini Nuri Aslami Nurul Aniqotun Mutmainah Putri Wardanis R. Fadly Bayu Dwiyoga Radella Hervidea, Radella Rahayu Amaliya Ratna Oktaviani Ratna Oktaviani Resta Tania Ria Aulia Noviantia Ria Aulia Noviantia, Ria Aulia Rina Maryani Risa Suryani Wilyasari Risma Rasmani Rochma Agustrina Rochmah Agustrina Rochmah Agustrina Rochmah Agustrina Rosa, Emantis RR. Ella Evrita Hestiandari Sally Khoirunisa Sally Khoirunisa Salman Farisi Sarah Sarah Selvi Marcelia Sembiring, Rinawati Setiawan, Alfian Bagus Setiawati, Primasari Linda Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sumardi Sumardi . Sumardi Sumardi Sumardi, Sumardi Suratman Suratman Suratman Umar Susanto, G. Nugroho Susanto, G. Nugroho Sutyarso Sutyarso Sutyarso Sutyarso Syavira Indriani T. Indah Setia Ningsih Tara Sesafia Paletri Tarisa Livia Hr Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Wanda Amelia Wardanis, Putri Widiastuti, Endang Linirin Widiastuti, Endang Linirin Yulianti Yulianty Yulianty Yulianty Soeradji Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yuniar, Lutfiah Zelfi Julita Dwi Putri Zulkifli Zulkifli Zulkifli zulkifli zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli