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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Kesehatan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Surya Medika Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Meditory : The Journal of Medical Laboratory Herb-Medicine Journal The Indonesian Journal of Infectious Diseases Jurnal Media Analis Kesehatan Jurnal Labora Medika Jurnal Ilmu dan Teknologi Kesehatan Journal of Health Sciences Jurnal Kesehatan Jurnal Analis Medika Biosains (JAMBS) Medical Technology and Public Health Journal Jurnal Kesehatan BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Pena Medika : Jurnal Kesehatan International Islamic Medical Journal Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Prosiding Seminar Nasional Hasil-hasil Penelitian dan Pengabdian Pada Masyarakat Jurnal Kesehatan Cendikia Jenius ASPIRATOR - Journal of Vector-Borne Diseases Studies Jurnal Kesehatan Jurnal Veteriner Jurnal Biodiversitas dan Bioteknologi
Dita Pratiwi Kusuma Wardani
UNIVERSITAS MUHAMMADIYAH PURWOKERTO
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Antibacterial Effectiveness of Beetroot (Beta vulgaris L.) Juice as an Alternative to Eosin for the Quality of Soil-Transmitted Helminth Egg Examination Yunita Nurmalasari; Ikhsan Mujahid; Dita Pratiwi Kusuma Wardani; M. Luthfi Almanfaluthi
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 1 (2025): Desember
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i1.335

Abstract

The 2% eosin reagent is commonly used in Soil Transmitted Helminth (STH) egg examination; however, its high cost, toxicity, and poor storage stability highlight the need for safer, natural-based alternatives. This study aimed to evaluate the effectiveness of beetroot (Beta vulgaris L.) juice as a substitute for eosin in staining STH eggs. A true experimental design with a post-test only control group was employed, consisting of six groups: one positive control (2% eosin) and five treatment groups using beetroot juice at concentrations of 40%, 50%, 60%, 80%, and 100%, with 11 preparations per group. Data collection was conducted through microscopic observation in July–August 2025 and analyzed using the Kruskal-Wallis test followed by the Mann-Whitney U post hoc test. The findings revealed significant differences in staining effectiveness (p = 0.027), with the 60% and 100% concentrations showing the best clarity and completeness of egg morphology. The study concludes that beetroot juice is an effective natural alternative to eosin. Further research is recommended to assess storage stability and explore other eco-friendly natural dyes.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH DAN DAUN MENGKUDU SEBAGAI BIOLARVASIDA TERHADAP LARVA NYAMUK Aedes aegypti Annisa Nur Chaeni; Ikhsan Mujahid; Dita Pratiwi Kusuma Wardani; Muhammad Lutfhi Almanfaluthi
Jurnal Kesehatan Vol. 15 No. 1 (2024)
Publisher : Sekolah Tinggi Ilmu Kesehatan (STIKes) Cirebon

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Abstract

Pengendalian nyamuk Aedes aegypti dengan menggunakan bahan kimia memiliki dampak negatif terhadap lingkungan dan kesehatan maupun organisme hidup. Upaya yang dapat dilakukan untuk meminimalisir dampak negatif yang ditimbulkan maka digunakan lavarsida alami dari tanaman yang memiliki potensi untuk membunuh Aedes aegpyti pada stadium larva. Penelitian ini bertujuan mengetahui efektivitas ekstrak bawang putih dan daun mengkudu sebagai biolarvasida terhadap mortalitas larva nyamuk Ae. aegypti. Penelitian ini merupakan penelitian true experimental dengan desain post test only with control group design. Sampel pada penelitian ini sebanyak 825 ekor. Teknik pengambilan sampel dilakukan dengan metode random sampling. Kelompok penelitian ini adalah temefos 1% dan aquadest, kelompok perlakuan ekstrak bawang putih (A. sativum) dan daun mengkudu (M. citrifolia) dengan masing – masing konsentrasi 0,2%, 0,4% dan 1%. Analisis data menggunakan uji Friedman, dilanjutkan dengan uji Post Hoc Mann-Whitney U dan analisis probit dilakukan untuk mengetahui nilai LC50 dan LC90. Setelah dilakukan olah data hasil penelitian menunjukan bahwa yang efektif dalam mortalitas larva 56% dan 30.4% dalam waktu 120 menit. Teirdapat peirbandingan antara eikstrak bawang puitih dan dauin meingkuidui seibagai biolarvasida teirhadap larva nyamuik Aei. aeigypti pada waktui peingamatan 120 meinit pada eikstrak bawang puitih yaitui 56% deingan meidian 14,00 (13-15) dan eikstrak dauin meingkuidui meincapai 30,4% deingan meidian 8,00 (7-8) seidangkan kombinasi eikstrak bawang puitih dan dauin meingkuidui yaitui 36% deingan meidian 9,00 (8-10), nilai LC50 dan LC90 ekstrak bawang putih adalah 0.135% dan 1.033% sedangkan nilai LC50 dan LC90 ekstrak daun mengkudu adalah 0.081% dan 1.621%. Bagi peneliti selanjutnya peirlui dilakuikan uiji kombinasi keiduianya deingan konseintrasi yang leibih tinggi dan seibaiknya dilakuikan uiji LT (Leithal Timei) sebagai larvasida alami yang ramah lingkungan.Kata Kunci: A. aegypti; Bawang Putih; Biolarvasida; Daun MengkuduAbstractControlling Aedes aegypti mosquitoes using chemical substances can have negative impacts on the environment, health, and other living organisms. To minimize these negative effects, natural larvicides derived from plants with the potential to kill Aedes aegypti larvae are utilized. This research aims to determine the effectiveness of garlic extract and noni leaves as bio-larvicides against the mortality of Aedes aegypti mosquito larvae. This true experimental research used a post-test-only control group design. The sample size was 825 larvae, selected through random sampling. The research groups included a temephos 1% and aquadest control group, a garlic extract (A. sativum) treatment group with concentrations of 0.2%, 0.4%, and 1%, and a noni leaves (M. citrifolia) treatment group with the same concentrations. Data analysis involved the Friedman test, followed by the Mann-Whitney U post-hoc test, and probit analysis to determine LC50 and LC90 values. The results show that the most effective treatment resulted in a larval mortality of 56% and 30.4% within 120 minutes. The LC50 and LC90 values for garlic extract are 0.135% and 1.033%, respectively, while for noni leaves, they are 0.081% and 1.621%. Future research is expected to further explore the potential of garlic and noni leaves as environmentally friendly natural larvicides.Keywords: A. aegypti; Garlic; Biolarvicides; Noni Leaves
Head Lice Mortality Activity (Pediculushumanus capitis) After Treating with Combination of Soursop Leaves Extract and Eucalyptus Oil HAMNA FATHIA ZAHRA; DITA PRATIWI KUSUMA WARDANI; ISNA HIKMAWATI; ATIKA NUR AZIZAH; IKHSAN MUJAHID; Muhammad Luthfi Almanfaluthi; OEI STEFANI YUANITA WIDODO; RETNO SULISTIYOWATI
Jurnal Veteriner Vol. 26 No. 3 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2025.26.3.320

Abstract

Pediculosis capitis is a scalp infection caused by the obligate ectoparasite Pediculus humanus capitis. This condition can be treated through various methods, including the use of medications. Soursop leaf extract and eucalyptus oil are known to exhibit lethal activity against P.h. capitis due to the presence of acetogenins, isoflavones, and cineole. This study aims to investigate the mortality activity of P.h. capitis following treatment with a combination of soursop leaf extract and eucalyptus oil. The study employed a true experimental design, specifically a post-test-only control group design, comprising two control groups (1% permethrin as the positive control (K+) and distilled water as the negative control (K-)) and five treatment groups with combinations of 5% soursop leaf extract and 5% eucalyptus oil (P1=1:1, P2=1:2, P3=2:1, P4=1:3, P5=3:1). The effectiveness of the combinations on P.h. capitis mortality was analyzed using Friedman, while the LC50 and LT50 values were determined through probit analysis. The results showed significant differences in mortality rates between the negative control group and the positive control group, as well as all treatment groups. Significant differences were also observed between the positive control group and the treatment groups P3, P4, and P5. However, no significant differences were found among the treatment groups at various observation times. The treatment results of group P2 (1:2) were the most effective in killing P. h. capitis. The combination of soursop leaf extract and eucalyptus oil has been proven effective as a natural pediculicide.
Soursop Leaves Extract Combined with Eucalyptus Oil: A Natural Strategy for Controlling Bed Bugs Firman Jaelani; Dita Pratiwi Kusuma Wardani; Isna Hikmawati; Atika Nur Azizah; Ikhsan Mujahid; Muhammad Luthfi Almanfaluthi; Oei Stefani Yuanita Widodo
Jurnal Veteriner Vol. 26 No. 4 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Insecticides used in controlling Bed bugs (Cimex lectularius)  often result in side effects and the development of resistance. Betel leaf (Piper betle Linn.), soursop leaf (Annona muricata Linn.), and Eucalyptus (Melaleuca cajuputi) oil have the potential to serve as natural insecticides due to their active ingredient content in the form of flavonoids, tannins, alkaloids and other bioactive compounds. This study aimed to evaluate the potency of ethanol extracts of betel leaf or soursop leaf in combination with cajeput oil in inducing mortality in C. lectularius. A true experimental design was employed using a post-test only with control group design, which included a positive control (2% bifenthrin), a negative control (distilled water), and 10 treatment groups with combinations of betel leaf or soursop leaf extracts with Eucalyptus oil in ratios of 1:1, 1:2, 2:1, 1:3, and 3:1. Each group consisted of 10 bed bugs, with three replications (totaling 360 bed bugs). Mortality was observed every five minutes over one hour and analysed using the Friedman test. The results indicated significant differences in the potential of the combinations of ethanol extracts and Eucalyptus oil. Probit analysis was used to determine LC₅₀ and LT₅₀ values. The LC₅₀ for the betel leaf and Eucalyptus oil combination was 1.302%, with an LT₅₀ of 0.976 minutes (ratio 1:1). For the soursop leaf and Eucalyptus oil combination, the LC₅₀ was 2.229%, and the LT₅₀ was 0.792 minutes (ratio 1:2). The fastest mortality was observed at five minutes in the combination of soursop leaf and Eucalyptus oil at a 3:1 ratio.
Studi Fenomenologis Pengembangan Kesehatan Melalui Program PKH di Kecamatan Banjaranyar Kabupaten Ciamis: Phenomenological Study of Health Development Through the PKH Program in Banjaranyar District, Ciamis Regency Mika'il, Hikam; Dhanti, Kurnia Ritma; Supriyadi; Wardani, Dita Pratiwi Kusuma
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 5 No. 7 (2022): July 2022
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v5i7.2433

Abstract

Latar Belakang: Kesehatan merupakan aspek terpenting dalam kehidupan seseorang, keluhan kesehatan pada seseorang dapat menurunkan produktivitas. Data sensus keluhan kesehatan pada tahun 2021, 27 dari 100 penduduk Indonesia mempunyai keluhan kesehatan dalam sebulan terakhir dan 13 dari 100 kegiatan sehari-hari menjadi terganggu akibat keluhan kesehatan. Tujuan: Pengembangan kesehatan melalui Program Keluarga Harapan (PKH) diharapkan mampu untuk meningkatkan produktivitas masyarakat, khususnya peserta PKH. Metode: Penelitian ini menggunakan jenis penelitian kualitatif dengan pendekatan fenomenologi. Hasil: Hasil penelitian berupa komponen input, proses, dan output. Seluruh hasil penelitian diolah secara deskriptif dan disampaikan dalam bentuk narasi disertai rangkuman berbentuk tabel. Kesimpulan: Upaya pengembangan kesehatan melalui program PKH berasal dari inovasi kebijakan yang mengedepankan penyempurnaan.
Efektivitas Ekstrak Bawang Putih (Allium sativum) Terhadap Mortalitas Larva Aedes aegypti : The Effectiveness of Garlic Extract (Allium sativum) Against the Mortality of Larvae Aedes aegypti Agesti, Eka Fauzia; Wardani, Dita Pratiwi Kusuma; Mujahid, Ikhsan; Hikmawati, Isna
Aspirator Vol 16 No 2 (2025): Jurnal Aspirator Volume 16 Nomor 2 2025
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58623/aspirator.v16i2.79

Abstract

Aedes aegypti is a dengue fever vector that can transmit dengue (DEN-V). Pyrethroid, carbamate, and organophosphate synthetic insecticides are often used as dengue control because they are very effective, quick to show results, and have minimal environmental impact. Garlic is commonly used as a seasoning by some people, but this plant has potential as an alternative larvicide. This plant contains the compounds allicin, sulfur ammonia acid, and allin. This study aims to determine the larvicidal effect of garlic extract on the mortality of Ae. aegypti larvae. The study was conducted with a post-test with a control group design of 5 groups. There are two groups: a negative control group (Aquadest) and a positive control (Temefos 1%). The other three groups were treated with garlic extract concentrations of 10% (P1), 20% (P2), and 30% (P3). There were 375 Ae. aegypti larvae with 3 repetitions, and 24 hours of larval mortality were observed. The Kruskal-Wallis and post hoc Mann-Whitney U tests analyzed larval mortality data. LC50 and LC90 values were subjected to probit analysis. The results of the Kruskal-Wallis test showed that there were differences in the effectiveness of garlic extracts on the mortality of Ae. aegypti larvae (p = 0.007). The LC50 value is 2.346%, while the LC90 value is 2.641%. Garlic extract (Allium sativum) is effective as a larvicide for Ae. aegypti
Kombinasi Ekstrak Bawang Merah (Alium ascalonicum) dan Minyak Kayu Putih (Melaleuca cajuputi) terhadap Mortalitas Pediculus humanus capitis: The Effectiveness of Shallot (Allium ascalonicum) Extract and Cajuput Oil (Melaleuca caju - puti) Combination on the Mortality of Pediculus humanus capitis Faza, Thariqi Mushthafa; Wardani, Dita Pratiwi Kusuma; Hikmawati, Isna; Azizah, Atika Nur; Mujahid, Ikhsan; Almanfaluthi, Muhammad Luthfi; Widodo, Oei Stefani Yuanita
Aspirator Vol 16 No 2 (2025): Jurnal Aspirator Volume 16 Nomor 2 2025
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58623/aspirator.v16i2.158

Abstract

 Pediculosis is caused by Pediculus humanus capitis and is characterized by scalp itching. The use of chemical pediculicides can cause scalp irritation and toxic hazards. The use of natural ingredients, such as a combination of shallot extract and eucalyptus oil, is used as an alternative pediculicide to reduce the impacts caused by the use of synthetic pediculicides. This research aims to determine the effectiveness of combining shallot extract and eucalyptus oil in eradicating head lice. This research is a true experimental type with a post-test only with control group design consisting of a positive control group (1% permethrin), a negative control (aquadest), and five treatment groups of 5% shallot extract combination and eucalyptus oil in ratios 1:1 (P1), 1:2 (P2), 2:1 (P3), 1:3 (P4), and 3:1 (P5). Data were analyzed using the Friedman test, and LT50 and LC50 were analyzed using probit analysis. There was a difference in the effectiveness of the 5% shallot extract combination and 5% eucalyptus oil. Group P4 was the most effective in causing mortality of Pediculus humanus capitis, with an LC50 value of 0.056% at 30 minutes, while the LT50 value was 0.781 minutes in group P4. The combination of shallot extract and eucalyptus oil in a 1:3 ratio was the most effective alternative to traditional plant-based pediculicides. The combination of shallot extract and eucalyptus oil has the potential to serve as an alternative pediculicide
Evaluasi Jumlah Monosit, Jumlah Limfosit dan Kadar Anti Streptolysin-O pada Pasien Tonsilitis Anak Hanny Dwi Indah Nurcahyani; Dita Pratiwi Kusuma Wardani; Arif Mulyanto; Kurnia Rhitma Dhanti
Jurnal Biodiversitas dan Bioteknologi Vol 2 No 2 (2025): Biodiverse: Jurnal Biodiversitas dan Bioteknologi
Publisher : Jurnal Biodiversitas dan Bioteknologi

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Abstract

Tonsillitis is the inflammation of the tonsils that can occur in children, caused by the bacteria Streptococcus haemolyticus and Streptococcus pyogenes. Poor oral hygiene, physical exhaustion, and certain types of food are other risk factors for chronic tonsillitis. When bacteria enter the tonsil tissue, monocytes differentiate into macrophages that destroy foreign objects. Activated lymphocytes differentiate into plasma cells that produce ASO antibodies. This study aims to determine the relationship between the number of monocytes and lymphocytes and Anti-Streptolysin-O levels in pediatric tonsillitis patients. This study was an observational analytical study with a cross-sectional design conducted in July-August 2025 at Dr. R. Goeteng Taroenadibrata Purbalingga Regional General Hospital by measuring the number of monocytes and lymphocytes using a hematology analyzer and Anti-Streptolysin-O levels using the latex agglutination method. The sample size for this study was 32 pediatric tonsillitis patients using accidental sampling. The relationship between monocyte and lymphocyte counts and ASO levels was analyzed using Spearman's rho correlation test. The results of the Spearman's rho correlation test showed that there was no correlation between the number of monocytes and ASO levels (p=0.637, r=0.087) or between the number of lymphocytes and ASO levels (p=0.412, r=-0.150). There was no correlation between the number of monocytes and lymphocytes and ASO levels in pediatric tonsillitis patients. More research is needed on inflammatory markers such as HsCRP, Procalcitonin (PCT) and Interleukin 6 (IL-6).
Evaluasi Jumlah Leukosit, Jumlah Netrofil, dan Kadar Anti Streptolysin O pada Pasien Tonsilitis Anak Citra Nadia Salsabila; Dita Pratiwi Kusuma Wardani; Arif Mulyanto; Defi Nurul Hayati
Jurnal Biodiversitas dan Bioteknologi Vol 2 No 2 (2025): Biodiverse: Jurnal Biodiversitas dan Bioteknologi
Publisher : Jurnal Biodiversitas dan Bioteknologi

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Abstract

Tonsillitis is a common global disease that most frequently affects children, particularly those aged 5–9 years. The primary cause of tonsillitis is infection of the tonsils by Streptococcus ?-hemolyticus Group A bacteria. Leukocytes play a key role in fighting infections, while neutrophils, as the main cells involved in acute inflammation, destroy bacteria through phagocytosis. This study aimed to determine the correlation between leukocyte and neutrophil counts and Anti-Streptolysin O (ASO) levels in children with tonsillitis. This research employed a cross-sectional design involving 32 pediatric tonsillitis patients, both inpatients and outpatients, at Dr. R. Goeteng Taroenadibrata Regional Hospital, Purbalingga. ASO levels were measured using the latex agglutination method, while hematological parameters—total leukocyte and neutrophil counts—were measured using a hematology analyzer based on the flow cytometry method. Data on the correlation between leukocyte and neutrophil counts and ASO levels were analyzed using Spearman’s rho correlation test. The results showed no significant correlation between leukocyte count and ASO level (p = 0.424, r = 0.146), and no correlation between neutrophil count and ASO level (p = 0.653, r = 0.083). There was no significant correlation between leukocyte and neutrophil counts and ASO levels in children with tonsillitis. Further studies are recommended using ASO measurement methods such as ELISA or turbidimetry, as well as additional inflammatory markers including Interleukin-1 (IL-1), Interferon-? (IFN-?), and Tumor Necrosis Factor-? (TNF-?).
Co-Authors Agesti, Eka Fauzia Almanfaluthi, Muhammad Lutfhi ALMANFALUTHI, MUHAMMAD LUTHFI Amalia Ovi Mustika Seno Ambarwati, Dewi Annisa Nur Chaeni Aprianto . Arif Mulyanto Arif Mulyanto Arif Mulyanto, Arif Atika Nur Azizah ATIKA NUR AZIZAH Azizah, Atika Nur Cahyaningrum, Winda Rahma Chaeni, Annisa Nur Citra Nadia Salsabila Defi Nurul Hayati Dewi Kristiya Nugra Heny Dhanti, Kurnia Ritma Dhanti, Kurnia Ritma Dina Aristya Purdiani EKANIASARI, MEVIANA DEA Farida Dwi Handayani Faza, Thariqi Mushthafa Firman Jaelani HAMNA FATHIA ZAHRA Handayani Narendo Putri handayani, Farida Hanny Dwi Indah Nurcahyani Heny, Dewi Kristiya Nugra Ika Wulan Nuri Anggreani Ikhsan Mujahid IKHSAN MUJAHID Ikhsan Mujahid Ikhsan Mujahid Indah Mulyani Ismi Andina Pertiwi Isna Hikmawati ISNA HIKMAWATI Isna Hikmawati Janatin Nur Aripin JANG JAYA, NABELA PUTRI Ketut Tirtayasa Kurnia Rhitma Dhanti Kurnia Ritma Dhanti Kurniawan Kurniawan Kurniawan Linda Wijayanti Listiyowati, Tanti M Luthfi Almanfaluthi M. Luthfi Almanfaluthi Mika'il, Hikam Minto Rahaju Minto Rahaju Muhammad Lutfhi Almanfaluthi Muhammad Luthfi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi MUJIONO, TIMBUL Nadia Ega Haliza Nuri Anggreani, Ika Wulan Nurul Badriyah Nurul Badriyah Nurul Qomariyah OEI STEFANI YUANITA WIDODO Oei Stefani Yuanita Widodo Oktafian Diyas Tamtama Olivia Alfitri Qo'imah, Alamanda Nurul Qo’imah, Alamanda Nurul Rahaju, Minto RETNO SULISTIYOWATI Retno Sulistiyowati RETNO SULISTYOWATI Retno Sulistyowati Retno Sulistyowati Ristiyanto Ristiyanto Ristiyanto ROHMAH, JAZILATUR Santi Safitri Shely Ayu Lestari Sudarsono, Tantri Analisawati SUDARSONO, TANTRI ANALISAWATI Supriyadi Supriyadi Supriyadi Syafira Dian Ergiana Syifa Arrachmah Tantowi, Linda Yanuana Fanida Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Widodo, Oei Stefani Yuanita Wijayanti, Linda Yunita Nurmalasari