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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Kesehatan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Surya Medika Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Meditory : The Journal of Medical Laboratory Herb-Medicine Journal The Indonesian Journal of Infectious Diseases Jurnal Media Analis Kesehatan Jurnal Labora Medika Jurnal Ilmu dan Teknologi Kesehatan Journal of Health Sciences Jurnal Kesehatan Jurnal Analis Medika Biosains (JAMBS) Medical Technology and Public Health Journal Jurnal Kesehatan BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Pena Medika : Jurnal Kesehatan International Islamic Medical Journal Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Prosiding Seminar Nasional Hasil-hasil Penelitian dan Pengabdian Pada Masyarakat Jurnal Kesehatan Cendikia Jenius Jurnal Kesehatan Jurnal Veteriner
Dita Pratiwi Kusuma Wardani
UNIVERSITAS MUHAMMADIYAH PURWOKERTO
Religion Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Antibacterial Effectiveness of Beetroot (Beta vulgaris L.) Juice as an Alternative to Eosin for the Quality of Soil-Transmitted Helminth Egg Examination Yunita Nurmalasari; Ikhsan Mujahid; Dita Pratiwi Kusuma Wardani; M. Luthfi Almanfaluthi
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 1 (2025): Desember
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i1.335

Abstract

The 2% eosin reagent is commonly used in Soil Transmitted Helminth (STH) egg examination; however, its high cost, toxicity, and poor storage stability highlight the need for safer, natural-based alternatives. This study aimed to evaluate the effectiveness of beetroot (Beta vulgaris L.) juice as a substitute for eosin in staining STH eggs. A true experimental design with a post-test only control group was employed, consisting of six groups: one positive control (2% eosin) and five treatment groups using beetroot juice at concentrations of 40%, 50%, 60%, 80%, and 100%, with 11 preparations per group. Data collection was conducted through microscopic observation in July–August 2025 and analyzed using the Kruskal-Wallis test followed by the Mann-Whitney U post hoc test. The findings revealed significant differences in staining effectiveness (p = 0.027), with the 60% and 100% concentrations showing the best clarity and completeness of egg morphology. The study concludes that beetroot juice is an effective natural alternative to eosin. Further research is recommended to assess storage stability and explore other eco-friendly natural dyes.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH DAN DAUN MENGKUDU SEBAGAI BIOLARVASIDA TERHADAP LARVA NYAMUK Aedes aegypti Annisa Nur Chaeni; Ikhsan Mujahid; Dita Pratiwi Kusuma Wardani; Muhammad Lutfhi Almanfaluthi
Jurnal Kesehatan Vol. 15 No. 1 (2024)
Publisher : Sekolah Tinggi Ilmu Kesehatan (STIKes) Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengendalian nyamuk Aedes aegypti dengan menggunakan bahan kimia memiliki dampak negatif terhadap lingkungan dan kesehatan maupun organisme hidup. Upaya yang dapat dilakukan untuk meminimalisir dampak negatif yang ditimbulkan maka digunakan lavarsida alami dari tanaman yang memiliki potensi untuk membunuh Aedes aegpyti pada stadium larva. Penelitian ini bertujuan mengetahui efektivitas ekstrak bawang putih dan daun mengkudu sebagai biolarvasida terhadap mortalitas larva nyamuk Ae. aegypti. Penelitian ini merupakan penelitian true experimental dengan desain post test only with control group design. Sampel pada penelitian ini sebanyak 825 ekor. Teknik pengambilan sampel dilakukan dengan metode random sampling. Kelompok penelitian ini adalah temefos 1% dan aquadest, kelompok perlakuan ekstrak bawang putih (A. sativum) dan daun mengkudu (M. citrifolia) dengan masing – masing konsentrasi 0,2%, 0,4% dan 1%. Analisis data menggunakan uji Friedman, dilanjutkan dengan uji Post Hoc Mann-Whitney U dan analisis probit dilakukan untuk mengetahui nilai LC50 dan LC90. Setelah dilakukan olah data hasil penelitian menunjukan bahwa yang efektif dalam mortalitas larva 56% dan 30.4% dalam waktu 120 menit. Teirdapat peirbandingan antara eikstrak bawang puitih dan dauin meingkuidui seibagai biolarvasida teirhadap larva nyamuik Aei. aeigypti pada waktui peingamatan 120 meinit pada eikstrak bawang puitih yaitui 56% deingan meidian 14,00 (13-15) dan eikstrak dauin meingkuidui meincapai 30,4% deingan meidian 8,00 (7-8) seidangkan kombinasi eikstrak bawang puitih dan dauin meingkuidui yaitui 36% deingan meidian 9,00 (8-10), nilai LC50 dan LC90 ekstrak bawang putih adalah 0.135% dan 1.033% sedangkan nilai LC50 dan LC90 ekstrak daun mengkudu adalah 0.081% dan 1.621%. Bagi peneliti selanjutnya peirlui dilakuikan uiji kombinasi keiduianya deingan konseintrasi yang leibih tinggi dan seibaiknya dilakuikan uiji LT (Leithal Timei) sebagai larvasida alami yang ramah lingkungan.Kata Kunci: A. aegypti; Bawang Putih; Biolarvasida; Daun MengkuduAbstractControlling Aedes aegypti mosquitoes using chemical substances can have negative impacts on the environment, health, and other living organisms. To minimize these negative effects, natural larvicides derived from plants with the potential to kill Aedes aegypti larvae are utilized. This research aims to determine the effectiveness of garlic extract and noni leaves as bio-larvicides against the mortality of Aedes aegypti mosquito larvae. This true experimental research used a post-test-only control group design. The sample size was 825 larvae, selected through random sampling. The research groups included a temephos 1% and aquadest control group, a garlic extract (A. sativum) treatment group with concentrations of 0.2%, 0.4%, and 1%, and a noni leaves (M. citrifolia) treatment group with the same concentrations. Data analysis involved the Friedman test, followed by the Mann-Whitney U post-hoc test, and probit analysis to determine LC50 and LC90 values. The results show that the most effective treatment resulted in a larval mortality of 56% and 30.4% within 120 minutes. The LC50 and LC90 values for garlic extract are 0.135% and 1.033%, respectively, while for noni leaves, they are 0.081% and 1.621%. Future research is expected to further explore the potential of garlic and noni leaves as environmentally friendly natural larvicides.Keywords: A. aegypti; Garlic; Biolarvicides; Noni Leaves
Head Lice Mortality Activity (Pediculushumanus capitis) After Treating with Combination of Soursop Leaves Extract and Eucalyptus Oil HAMNA FATHIA ZAHRA; DITA PRATIWI KUSUMA WARDANI; ISNA HIKMAWATI; ATIKA NUR AZIZAH; IKHSAN MUJAHID; Muhammad Luthfi Almanfaluthi; OEI STEFANI YUANITA WIDODO; RETNO SULISTIYOWATI
Jurnal Veteriner Vol. 26 No. 3 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2025.26.3.320

Abstract

Pediculosis capitis is a scalp infection caused by the obligate ectoparasite Pediculus humanus capitis. This condition can be treated through various methods, including the use of medications. Soursop leaf extract and eucalyptus oil are known to exhibit lethal activity against P.h. capitis due to the presence of acetogenins, isoflavones, and cineole. This study aims to investigate the mortality activity of P.h. capitis following treatment with a combination of soursop leaf extract and eucalyptus oil. The study employed a true experimental design, specifically a post-test-only control group design, comprising two control groups (1% permethrin as the positive control (K+) and distilled water as the negative control (K-)) and five treatment groups with combinations of 5% soursop leaf extract and 5% eucalyptus oil (P1=1:1, P2=1:2, P3=2:1, P4=1:3, P5=3:1). The effectiveness of the combinations on P.h. capitis mortality was analyzed using Friedman, while the LC50 and LT50 values were determined through probit analysis. The results showed significant differences in mortality rates between the negative control group and the positive control group, as well as all treatment groups. Significant differences were also observed between the positive control group and the treatment groups P3, P4, and P5. However, no significant differences were found among the treatment groups at various observation times. The treatment results of group P2 (1:2) were the most effective in killing P. h. capitis. The combination of soursop leaf extract and eucalyptus oil has been proven effective as a natural pediculicide.
Soursop Leaves Extract Combined with Eucalyptus Oil: A Natural Strategy for Controlling Bed Bugs Firman Jaelani; Dita Pratiwi Kusuma Wardani; Isna Hikmawati; Atika Nur Azizah; Ikhsan Mujahid; Muhammad Luthfi Almanfaluthi; Oei Stefani Yuanita Widodo
Jurnal Veteriner Vol. 26 No. 4 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Insecticides used in controlling Bed bugs (Cimex lectularius)  often result in side effects and the development of resistance. Betel leaf (Piper betle Linn.), soursop leaf (Annona muricata Linn.), and Eucalyptus (Melaleuca cajuputi) oil have the potential to serve as natural insecticides due to their active ingredient content in the form of flavonoids, tannins, alkaloids and other bioactive compounds. This study aimed to evaluate the potency of ethanol extracts of betel leaf or soursop leaf in combination with cajeput oil in inducing mortality in C. lectularius. A true experimental design was employed using a post-test only with control group design, which included a positive control (2% bifenthrin), a negative control (distilled water), and 10 treatment groups with combinations of betel leaf or soursop leaf extracts with Eucalyptus oil in ratios of 1:1, 1:2, 2:1, 1:3, and 3:1. Each group consisted of 10 bed bugs, with three replications (totaling 360 bed bugs). Mortality was observed every five minutes over one hour and analysed using the Friedman test. The results indicated significant differences in the potential of the combinations of ethanol extracts and Eucalyptus oil. Probit analysis was used to determine LC₅₀ and LT₅₀ values. The LC₅₀ for the betel leaf and Eucalyptus oil combination was 1.302%, with an LT₅₀ of 0.976 minutes (ratio 1:1). For the soursop leaf and Eucalyptus oil combination, the LC₅₀ was 2.229%, and the LT₅₀ was 0.792 minutes (ratio 1:2). The fastest mortality was observed at five minutes in the combination of soursop leaf and Eucalyptus oil at a 3:1 ratio.
Studi Fenomenologis Pengembangan Kesehatan Melalui Program PKH di Kecamatan Banjaranyar Kabupaten Ciamis: Phenomenological Study of Health Development Through the PKH Program in Banjaranyar District, Ciamis Regency Mika'il, Hikam; Dhanti, Kurnia Ritma; Supriyadi; Wardani, Dita Pratiwi Kusuma
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 5 No. 7 (2022): July 2022
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v5i7.2433

Abstract

Latar Belakang: Kesehatan merupakan aspek terpenting dalam kehidupan seseorang, keluhan kesehatan pada seseorang dapat menurunkan produktivitas. Data sensus keluhan kesehatan pada tahun 2021, 27 dari 100 penduduk Indonesia mempunyai keluhan kesehatan dalam sebulan terakhir dan 13 dari 100 kegiatan sehari-hari menjadi terganggu akibat keluhan kesehatan. Tujuan: Pengembangan kesehatan melalui Program Keluarga Harapan (PKH) diharapkan mampu untuk meningkatkan produktivitas masyarakat, khususnya peserta PKH. Metode: Penelitian ini menggunakan jenis penelitian kualitatif dengan pendekatan fenomenologi. Hasil: Hasil penelitian berupa komponen input, proses, dan output. Seluruh hasil penelitian diolah secara deskriptif dan disampaikan dalam bentuk narasi disertai rangkuman berbentuk tabel. Kesimpulan: Upaya pengembangan kesehatan melalui program PKH berasal dari inovasi kebijakan yang mengedepankan penyempurnaan.
Co-Authors Almanfaluthi, Muhammad Lutfhi Amalia Ovi Mustika Seno Ambarwati, Dewi Annisa Nur Chaeni Aprianto . Arif Mulyanto Arif Mulyanto Arif Mulyanto, Arif Atika Nur Azizah ATIKA NUR AZIZAH Cahyaningrum, Winda Rahma Chaeni, Annisa Nur Dewi Kristiya Nugra Heny Dhanti, Kurnia Ritma Dhanti, Kurnia Ritma Dina Aristya Purdiani EKANIASARI, MEVIANA DEA Farida Dwi Handayani Firman Jaelani HAMNA FATHIA ZAHRA Handayani Narendo Putri handayani, Farida Heny, Dewi Kristiya Nugra Ika Wulan Nuri Anggreani Ikhsan Mujahid Ikhsan Mujahid IKHSAN MUJAHID Ikhsan Mujahid Indah Mulyani Ismi Andina Pertiwi Isna Hikmawati ISNA HIKMAWATI Isna Hikmawati Janatin Nur Aripin JANG JAYA, NABELA PUTRI Ketut Tirtayasa Kurnia Ritma Dhanti Kurniawan Kurniawan Kurniawan Linda Wijayanti Listiyowati, Tanti M Luthfi Almanfaluthi M. Luthfi Almanfaluthi Mika'il, Hikam Minto Rahaju Minto Rahaju Muhammad Lutfhi Almanfaluthi Muhammad Luthfi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi MUJIONO, TIMBUL Nadia Ega Haliza Nuri Anggreani, Ika Wulan Nurul Badriyah Nurul Badriyah Nurul Qomariyah Oei Stefani Yuanita Widodo OEI STEFANI YUANITA WIDODO Oktafian Diyas Tamtama Olivia Alfitri Qo'imah, Alamanda Nurul Qo’imah, Alamanda Nurul Rahaju, Minto RETNO SULISTIYOWATI Retno Sulistiyowati Retno Sulistyowati RETNO SULISTYOWATI Retno Sulistyowati Ristiyanto Ristiyanto Ristiyanto ROHMAH, JAZILATUR Santi Safitri Shely Ayu Lestari SUDARSONO, TANTRI ANALISAWATI Sudarsono, Tantri Analisawati Supriyadi Supriyadi Supriyadi Syafira Dian Ergiana Syifa Arrachmah Tantowi, Linda Yanuana Fanida Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Wijayanti, Linda Yunita Nurmalasari