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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Agrikultura Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar AL KAUNIYAH Techno: Jurnal Penelitian AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology AJIE (Asian Journal of Innovation and Entrepreneurship) UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) ANNALES BOGORIENSES Agrin : Jurnal Penelitian Pertanian Warta Pengabdian Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Karinov Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Journal of Community Development BIOVALENTIA: Biological Research Journal PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia Agricultural Power Journal Jurnal Pengabdian Masyarakat IPTEKS
Parawita Dewanti
Jember University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Vegetalika

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Optimasi Optical Density (OD) Dan Dosis Kanamisin Dalam Transformasi Gen Sucrose Phosphate Synthase (SoSPS1) Pada Anggrek Dendrobium sp. Arsy Chairiyah; Bambang Sugiharto; Purnama Okviandari; Parawita Dewanti; Khiar Ayatina Hasbi
Vegetalika Vol 14, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106229

Abstract

Genetic transformation in plants involves the insertion of isolated foreign genes into the plant genome. Sucrose Phosphate Synthase (SPS) is a key enzyme in sucrose biosynthesis, the primary sugar serving as an energy source and carbon transport molecule in most plants. Dendrobium orchids, which utilize Crassulacean Acid Metabolism (CAM), often exhibit a slow growth rate, thereby limiting their commercial potential. The insertion of the SPS gene into the orchid genome is expected to optimize the sucrose metabolic pathway, potentially influencing the rate of photosynthesis, carbon allocation, and growth characteristics of the orchid. Optimizing the optical density (OD) of the Agrobacterium suspension and the dosage of kanamycin is crucial for enhancing the success rate of the gene transformation process. This study aimed to determine the optimal concentrations of OD and kanamycin to achieve efficient SoSPS1 gene transformation in orchids. Three-month-old Protocorm-Like Bodies (PLBs) of Dendrobium Glory White were used as the explant source. The transformed explants were subsequently evaluated for the effectiveness of the kanamycin dosage, the percentage of explant shoots, the number of shoots per explant, the transformation efficiency, and phenotypic changes in the transformed plants. The results showed that a transformation efficiency of up to 40% was achieved using an Agrobacterium suspension at an OD600nm of 0.4. A lethal dose (LD50) of 100 mgL-1 kanamycin resulted in 34% of plantlets surviving post-selection. Transformation with the SoSPS1 gene in Dendrobium sp. induced distinct phenotypic plants including split leaves, swollen stems (pseudobulb), and increased shoot multiplication.
Co-Authors . Usmadi Abdul Jalil Abdul jalil Adinda, Intan Dwi Ahnaf, Yusuf Dary Al Ayyubi, Nabila Nur Aisyah Alfian, Firdha Narulita Alifah Farida Sa’adah Alifatul Aqidah Arsy Chairiyah Arya Wiranegara Azmi Saleh Bagus Tripama Bambang Soegiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kristanto Budi Kriswanto Cesha Ananda Putri Desi Kartika Sutrisno Dwi Setyati Dwika Nano Hariyanto Erma Sulistyaningsih Fanata, Wahyu Indra Duwi Firdausi, Intan Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Hakim, Mohammad Sulton Harsanti, Restiani Sih Hidayat Hidayat Hidayat Ika Purnamasari Ika Purnamasari Indraloka, Aldy Bahaduri Intan Dwi Ambarwati Inyana Dwi Agustien Iryono Kacung Hariyono Khiar Ayatina Hasbi Khofifa, Rendryana Aulia Nur Khozin, Mohammad Nur Laela Endah Rahmadhani Laily Ilham Widuri Laily Ilman Widuri, Laily Ilman Lenny Widjayanthi Magfiroh, Illia Seldon Maisaro, Maisaro Maryam, Safira Arikha milga sari Mohammad Ali Mudhor Mohammad Ubaidillah Mona, Muhammad Dima Say Muhammad Hazmi Munandar, Denna Eriani Nadiya, Nisma Riyadh Nafisah Iqmatullah Ni Made Ayu Gemuh Rasa Astiti Nina Oktaria Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Olandino Tome Francisco Dorosario de Sousa Popy Hartatie Hardjo Pralampita, Pulong Wijang Prayoga, Mohammad Candra Purnama Okviandari Purnama Okviandari Purnama Okviandari, Purnama Puspito, Agung Nugroho Putri, Cesha Ananda Putri, Widya Kristyanti Raden Soedradjad Rahadian Falqi Taufik Rahmadhani, Laela Endah Raisah Bani Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Ristiyana, Suci Rizky Harikurniawan Rudi Joelijanto Santoso, Luthfi Nur Aprillia Sasmita, Heni Dwi Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Siti Kamalia Siti Nurul Afidah Slameto Slameto Slameto Slameto Soegiharto, Bambang Sri Hartatik Suci Ristiyana Sulistiyono Syafira Fatihatul Husna Taufik, Rahadian Falqi Tri Agus Siswoyo Tri Agus Siswoyo TRI HANDOYO Tri Wahyu Saputra Tri Wahyu Saputra Ubaidillah, Mohammad Ummi Solikhah, Ummi Veronenci Yuliarbi Farlisa Wachju Subchan Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Wakhidaturrohmah, Ninik Wardatus Sholeha Widuri, Laily Ilham Widya Kristiyanti Putri Wulan Nursyiam Ningtiyas Wulanjari, Distiana Yani Corvianindya Rahayu