Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Biota

Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck) Septarini Dian Anitasari; Ida Ayu Astarini; Made Ria Defiani; Made Pharmawati .; Dian Catur Prayantini
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3340

Abstract

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important. The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture. The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore. Pollen viability was determined by IKI staining and pollen germination method. Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development. Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture. Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’.
Co-Authors A A Ayu Laksmi Damarnegari A.A.S. A. Sukmaningsih Ahmad Zakir Andrianus Sembiring Andrianus Sembiring Angel Chappell Anitasari, Septarini Dian Astria Yusmalinda Austin Ryan Garrido Catarina Tenny Setiastri Cornelia Coraima Lazaren Danie Al Malik Debora Margareth Debora Margareth Dian Catur Prayantini Douglas Scheuring Dwi Nur Rikhma Sari Edelmiro Jose De Deus Elok Faiqoh Ema Hendriyani Enex Yuniarti Ningsih Eniek Kristiyanti ENIEK KRISWIYANTI GUIJUN YAN Gusti Ayu Nyoman Budiwati Hermanto, Bagus I Dewa Gede Warmadewa I Dewa Kadek Wira Sanjaya I G. R. M. TEMAJA I Gede Mahardika I Gusti Ayu Putri Kartika, I Gusti Ayu I Gusti Ayu Sugi Wahyuni I Gusti Bagus Sila Dharma I Gusti Bagus Sila Dharma I Gusti Made Oka Nurjaya I GUSTI NGURAH SANTOSA I Ketut Catur Marbawa I Ketut Ginantra I Made Antara I Made Saka Wijaya I Nyoman Gede Wisesa Adnyana I NYOMAN MERIT I NYOMAN RAI I W BUDIARSA SUYASA I Wayan Arthana I Wayan Gede Astawa Karang I Wayan Krisna Eka Putra I Wayan Krisna Eka Putra I Wayan Suarna I WAYAN WINDIA I.G.M Oka Nurjaya I.M.S. Wijaya Ida Ayu Putri Darmawati Ida Bagus Wayan Gunam Indah Tria Hoky Jennifer Crismonika Jeremy Pramana Josina Irene Brigita Hutubessy JULIAN CREIGHTON MILLER JR Julian Creighton Miller Jr. JULIE ANNE PLUMMER Junita Hardini Kadek Andina Widiastuti Kadek Andina Widiastuti Kriviņš, Anatolijs Luh Astria Yusmalinda Luh Gede Artha Saridewi Wijaya Luh Made Arpiwi LUH Puru ESWARYANTI KUSUMA YUNI M. Danie Al Malik MADE PHARMAWATI Made Ria Defiani Made Wahyu Cerianingsih Maghfirotun Nisa Meitini Proborini Wahyuni Mia Kosmiatin Miller, Jr, J. Creighton Mohammad Subhan Muhammad Danie Al Malik Muhammad Ibadullah Ni Kade Ayu Purnama Adi Ni Kadek Dwipayani Lestari Ni Kadek Raleni Ni Kadek Sari Dewi Handayani NI KADEK YUNITA SARI Ni Ketut Mas Suratniasih Ni LUH ARPIWI Ni Luh Astria Yusmalinda Ni Luh Putu Kayika Febryanti Ni Luh Suriani Ni Made Ary Widiastiti NI MADE DIAN PRATIWI Ni Made Puspawati NI NYOMAN ARI MAYADEWI Ni Nyoman Ely Kristiyanti Ni Nyoman Nila Arieswari Ni Nyoman Nila Arieswari Ni Putu Adriani Astiti Ni Putu Adriani Astiti Ni Putu Anom Sulistiawati, Ni Putu Anom Ni Putu Dian Pertiwi Ni Putu Vivin Nopiantari Ni Putu Yuni Astriani Dewi Ni Wayan Deswiniyanti Putra, I Nyoman Giri Putu Dian Pertiwi Putu Dian Pertiwi Putu Gede Arya Sumertayasa Putu Kayika Febryanti, Ni Luh Putu Wina Andriani Lestari Rindang Dwiyani SANG KETUT SUDIRGA Sean Michael Thompson Sebastian S. Cocioba Septarini Dian Anitasari Septarini Dian Anitasari Septarini Dian Anitasari Shella Ayu Ardiana Suryany Devy Taebenu, Desi Adriyanti Nina Tarigan, Swary Dwi Suherna Tiwi Wati VEMY CLAUDIA Wildan Mujahidul Basyar Yuli Setiawati YUNALDI YUNALDI YUNITA HARDINI Zaenul Gafari