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All Journal Jurnal Sains dan Teknologi Journal of Information Systems Engineering and Business Intelligence Tropical Animal Science Journal bit-Tech Jurnal Cahaya Mandalika Hukum Responsif : Jurnal Ilmiah Fakultas Hukum Universitas Swadaya Gunung Jati Cirebon Journal of Science and Education (JSE) Abdi-Dharma East Asian Journal of Multidisciplinary Research (EAJMR) Jurnal Agribis Journal Of Science Education And Management Business Bandung Conference Series: Accountancy JURNAL PILAR KEADILAN Journal of Research and Publication Innovation MAJAPAHIT POLICY Jurnal Industrikrisna Jurnal Krakatau (Indonesian of Multidiciplinary Journal) AGRIC Jurnal Ekonomi Bisnis Manajemen dan Akuntansi (Jebisma) Jurnal Pengabdian Masyarakat dan Riset Pendidikan Al-Kharaj: Jurnal Ekonomi, Keuangan & Bisnis Syariah El-Mal: Jurnal Kajian Ekonomi & Bisnis Islam LEX LAGUENS: Jurnal Kajian Hukum dan Keadilan Journal of Ekonomics, Finance, and Management Studies Indonesian Journal of Humanities and Social Sciences STIKes BBM Mengabdi: Jurnal Pengabdian Masyarakat Journal Social Civilecial Jurnal Pendidikan Islam
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Journal : Tropical Animal Science Journal

 1 
The Quality of Cryopreserved Semen of Local Chickens Treated with Ringer’s Lactate-Egg Yolk Extender Supplemented with Glycine and Glucose Junaedi; Isnaini, N.; Natsir, M. H.; Susilawati, T.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.161

Abstract

The aim of this study is to investigate the potential of glucose, glycine, and a combination of glucose and glycine supplemented into Ringer’s lactate extender on the quality of pre- and post-cryopreservation of semen of local chickens. Semen samples were collected from KUB roosters. The research procedures involved formulation of the extender, semen collection, assessment of fresh semen, dilution, packaging, cryopreservation, storage, and subsequent assessment of cryopreserved semen. Parameters assessed in fresh, pre-cryopreservation, and post-thawed semen using computer-assisted semen analysis (CASA), included motility (total motility, progressive, static, and slow motilities), kinematics, spermatozoa morphology, malondialdehyde (MDA) concentration, and scanning electron microscopy (SEM). There were four treatments: T1= control, T2= 50 mM glucose, T3= 60 mM glycine, and T4= a combination of 50 mM glucose and 60 mM glycine. Each treatment was repeated 10 times. The results showed that the addition of 60 mM glycine and the combination of 50 mM glucose and 60 mM glycine effectively preserved total motility, maintained progressive motility, and reduced damage to the bent tail during the equilibration process (prior to cryopreservation). In addition, total motility, progressive motility, DAP, DSL, DCL, VAP, VSL, VCL, STR, and LIN were significantly increased after thawing. This approach also minimized the formation of MDA. Furthermore, observations using SEM showed comparatively more intact acrosomes compared to the other treatments. In conclusion, the addition of 60 mM glycine and a combination of 60 mM glycine and 50 mM glucose to Ringer’s lactate-egg yolk extender proved to be effective in preserving the quality of domestic chicken spermatozoa during semen cryopreservation.
The Quality of Gaga Roosters Semen During Cold Storage Using a Diluent Supplemented with Sorbitol Khaeruddin; Ciptadi, G.; Yusuf, M.; Fattah, A. H.; Junaedi; Syamsuryadi, B.; Wahjuningsih, S.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.436

Abstract

This study aimed to examine the effect of adding different concentrations of sorbitol to the Ringer’s lactate-egg yolk (RLEY) diluent on the quality of Gaga roosters’ semen during cold storage. A completely randomized design was used with 10 replicates and 4 levels of diluent treatment, including control, 1%, 2%, and 3% sorbitol. Semen was collected using a massage method, followed by fresh semen evaluation and dilution with a ratio of 1:5. Semen was stored at a cold temperature (5 ºC) and observed after 0, 24, 48, and 72 hours. The variables observed were semen pH, motility, kinematics, viability, plasma membrane integrity, acrosome integrity, malondialdehyde (MDA), and intracellular calcium ion (Ca2+) concentrations of sperm. All data were analyzed using analysis of variance (ANOVA). Subsequently, the evaluation of sperm mitochondrial activity and DNA damage was carried out, and the data were analyzed using the T-test. The results showed that treatment with sorbitol in the diluent had no effect on semen pH, kinematics (except straightness), acrosomal cap integrity, DNA damage, mitochondrial activity, and MDA concentration. However, sorbitol concentrations significantly affect (p<0.05) total motility, progressive motility, static motility, STR, viability, plasma membrane integrity, and intracellular Ca2+. In conclusion, the addition of 1-3% sorbitol preserves the quality of Gaga roosters’ semen during 72 hours of cold storage.
Co-Authors Abdul Wahab Aditiya Hermawan Ahmad Sukandar Ahmad Taufik Ahmad Yusuf Andrian Putra Ambarsari, Dewi Ananda, Stanley Anang Rohmad Jatmiko Andri Wijaya Arman Asnun Parwanti Bachtiar, Machdum Bachtillah, Andriansyah Benny Daniawan Daniawan, Benny Dede Andi Dewi Oktavia Diki Dikrurahman edy Fattah, A. H. Fauzi, Agam G. Ciptadi Habeahan, Rasman Halim Wijaya, Ardie Hanun Asrohah Hendra Gunawan, Alexius Hermawan , Aditiya Irninthya Nanda Pratami Irwan Iskandar Ritonga Johan Santoso Jonathan Junaenah Khaeruddin Kristen Kumala, Sonya Ayu Kurnia , Yusuf Kurniawan Maranto, Ardiane Rossi Kuswanto, Verri Lunardi, Lidya M. Yusuf Machrus Ali Mawar Kartina, Ratu Mey Maemunah Mone, Ansyari Muh Nasir Muhamad Ali Muhammad Afandi Arifin N. Isnaini Nailatuzzuhra Natsir, M. H. Nurhapsa Nuridha Rachmat, Ade Dian Pupung Purnamasari Risnandar, Aep Robertus Basiya Rosita, Yeti Rossi , Ardiane Rossi Kurniawan Maranto, Ardiane Rossi Ryo Setyawan S. Wahjuningsih Sugeng Prayitno Supriyatno Suwitno Syahid, Adi Syahruni Thamrin, Syahruni Syamsuryadi, B. T. Susilawati Tia Nurapriyanti Yusuf Kurnia Yusuf Kurnia, Yusuf Zohriah, Anis