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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Seminar Nasional Aplikasi Teknologi Informasi (SNATI) Agrotrop : Journal on Agriculture Science Jurnal Agrista JIK Jurnal Ilmu Komputer BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Cropsaver : Journal of Plant Protection Jurnal Algoritma LISANIA Jurnal Ilmu dan Pendidikan Bahasa Arab Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains OPSI AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya agriTECH JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Kebijakan dan Pelayanan Publik Law and Justice Jurnal Matematika Sains dan Teknologi Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Teknik Informatika STMIK Antar Bangsa Jurnal Ergonomi dan K3 Jurnal Agrotek Tropika Jurnal Pengabdian Masyarakat AbdiMas JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Journal of Tropical Crop Science Jurnal Ilmiah Pendidikan Scholastic JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan WIDYA LAKSANA Ensiklopedia of Journal Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Bidan Cerdas Jurnal Sains dan Teknologi Reaksi Jurnal Kebidanan Mutiara Mahakam Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Sains Agro Jurnal ABDIKU Indian Journal of Forensic Medicine & Toxicology Indonesian Science Education Research (ISER) Jurnal Konstitusi Jurnal Teknologi Kimia Unimal Jurnal Algoritma Jurnal Penelitian Kehutanan Wallacea
Nurul Khumaida
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia
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Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid Ika Roostika; Ika Mariska; Nurul Khumaida; Gustaaf A. Wattimena
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p8-18

Abstract

This research aimed to study the effect of 2,4-D,AdS, and basal media to the regeneration of pineapplethrough indirect organogenesis and somatic embryogenesis,and to study the complete event of somatic embryogenesis.Callus formation was induced by 21, 41, and 62 μM 2,4-Dwith addition of 9 μM TDZ. The non embryogenic calli weretransferred onto 4.65 μM Kn containing medium.Embryogenic callus formation was induced on MS or Bacbasal media consisted of N-organic compounds withaddition of AdS (0, 0.05 and 0.1 μM). The embryogenic calliwere regenerated on modified MS medium with addition of0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS mediumsupplemented with 0.018 mM BA. The result proved that thesingle auxin of 2,4-D was not enough to induce embryogeniccells. Therefore the non embryogenic calli were regeneratedthrough organogenesis. The 21 μM 2,4-D yielded high level ofcallus formation (80%), higher fresh weight (0.2 g/explant)and higher number of shoot (25 shoots/explant in twomonths). Embryogenic calli were produced on N-organiccompounds enriched media. The regeneration mediumsignificantly affected the level of browning, where the MSmedium with addition of 0.018 mM BA yielded lower level ofbrowning. There was an interaction of embryogenic callusinduction medium and regeneration medium to the numberof mature somatic embryos. The embryogenic callusinduction on MS medium enriched with N-organiccompounds and 0.05 μM AdS followed by the regenerationof somatic embryos on MS medium with addition of 0.018mM BA was the best treatment which yielded 17 maturesomatic embryos/explant.
Optimasi Regenerasi Padi Indica melalui Jalur Organogenesis Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 13, No 1 (2017): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n1.2017.p35-42

Abstract

Regenerasi tanaman merupakan tahapan penting yang perlu dikuasai sebelum diaplikasikan untuk perakitan varietas unggul secara kultur in vitro. Penelitian bertujuan mengoptimasi teknik regenerasi in vitro beberapa varietas padi indica melalui jalur organogenesis. Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial. Materi yang dicobakan, yaitu Ciherang, Inpari 13, Inpara 3, Pokkali, dan IR29. Perlakuan yang diberikan untuk induksi kalus organogenik adalah 2,4-D (0, 1, 3, 5, 7 mg/l) + kasein hidrolisat 3 g/l, untuk regenerasi kalus membentuk tunas adventif adalah BA (0, 1, 5 mg/l) + zeatin (0, 0,1, 0,3 mg/l) + prolin 100 mg/l, untuk multiplikasi tunas adalah MS + thidiazuron/TDZ (0, 0,1, 0,3 mg/l) dan untuk perakar-an adalah IBA (0, 1, 2, 3 mg/l). Hasil penelitian menunjukkan media terbaik untuk induksi kalus untuk padi varietas Ciherang, Inpari 13, dan Pokkali adalah MS + 2,4-D 3 mg/l, sedangkan untuk varietas Inpari 3 dan IR 29 adalah MS + 2,4-D 5 mg/l. Media terbaik untuk regenerasi tunas untuk varietas Ciherang adalah BA 5 mg/l + zeatin 0,1 mg/l + prolin 100 mg/l, untuk Inpari 13 adalah MS + BA 3 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l, untuk Inpara 3 adalah BA 5 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l dan untuk Pokkali dan IR29 adalah MS + BA 3 mg/l + zeatin 0,1 mg/l. Media terbaik untuk multiplikasi tunas adalah MS + TDZ 0,3 mg/l dan untuk induksi perakaran adalah MS + IBA 2 mg/l. Setiap tahapan kegiatan pembentukan planlet membutuhkan formulasi media yang berbeda.
KUTU TANAMAN DAN TRIPS BERASOSIASI DENGAN TANAMAN DAUN UNGU DAN TINGKAT KERUSAKAN TANAMAN Tri Lestari Mardiningsih; Dewi Sartiami; Nurul Khumaida; Natalini Nova Kristina; Cucu Sukmana
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 1 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n1.2012.%p

Abstract

Daun ungu (Graptophyllum pictum) atau dalam bahasa Sunda : handeuleum merupakan salah satu tanaman yang berkhasiat untuk mengobati penyakit wasir, melancarkan buang air seni, melancarkan haid dan lain-lain. Salah satu kendala dalam budidaya tanaman ialah serangan ulat pemakan daun. Ulat ini sangat rakus sehingga tanaman yang diserang dapat menjadi gundul. Ulat pemakan daun yang menyerang tanaman daun ungu di Jawa Barat ialah Doles-challia bisaltide, sedang di Maluku dan Papua masing-masing Doleschallia nacar dan Doleschallia hexophtalmos. Selain serangga tersebut juga terdapat kutu tanaman dan trips yang berasosiasi dengan tanaman daun ungu. Untuk mengetahui kutu tanaman dan trips tersebut maka dilakukan identifikasi se-rangga-serangga tersebut yang beraso-siasi dengan tanaman daun ungu dari Jawa Barat, Maluku, dan Papua sejak April sampai Juni 2010. Kutu tanaman dan trips, dikoleksi dengan memasukkan masing-masing ke dalam botol gelas yang berisi alkohol 70%, dibuat preparat mikroskop di laboratorium, kemudian diidentifikasi dengan menggunakan buku kunci identifikasi. Selain itu, juga dilaku-kan pengamatan serangan kutu tanaman utama yang menyerang daun ungu. Hasil identifikasi menunjukkan bahwa kutu tanaman dan trips yang berasosiasi dengan tanaman daun ungu ialah Rastro-coccus viridarii Williams (Hemiptera : Pseudococcidae), kututempurung Sais-setia neglecta De Lotto (Hemiptera : Coccidae), Insignorthezia insignis Browne (Hemiptera : Ortheziidae), kutudaun Aphis gossypii Glover (Hemiptera : Aphididae), dan Astrothrips tumiceps Karny (Thysanoptera : Thripidae). Serangan kututempurung S. neglecta di rumah kaca pada tanaman muda dapat menyebabkan kematian tanaman daun ungu.
Implementation Of Laws On The Criminal Jurisdiction System Using A Restorative Justice Approach (A Case Study at ‘Aisyiyah Legal A id Institute, Central Java) Zaenal Aripin
Law and Justice Vol 5, No 2 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/laj.v5i2.11267

Abstract

This article aims to determine the implementation of the juvenile criminal justice system law in upholding child criminal law in Surakarta, explain the process of assistance for victims and child offenders who are dealing with the law with a restorative justice approach in the form of diversion, and explain the obstacles found LBH (Legal Aid) 'Aisyiyah Central Java in law enforcement which acts as a child crime in Surakarta. This study uses an empirical approach. This research was conducted by examining primary data in the field, namely LBH Aisyiyah Central Java in the form of facts about the results of research directly at the research location and the results of interviews with members of advocacy organizations at LBH 'Aisyiyah, Central Java and in the Legal and Human Rights Council of' Aisyiyah Regional Leaders. Central Java and secondary data. The results showed that based on the results of the study in 10 criminal cases committed by children who were resolved by diversion in Surakarta, they had fulfilled and were in accordance with the Articles regulating diversion in the SPPA Law.  Whereas the concept of diversion has been running in Surakarta with many determinations of the results of a diversion agreement between the families of the victims and the families of the perpetrators and also LBH (Legal Aid) or related LSM (Non-Govermental Organization)  for crimes committed by minors. So that the application of the Law on Juvenile Criminal Justice System in law enforcement of child criminal acts has been running quite effectively.  Keywords: SPPA Law, diversion, restorative justice, Surakarta
Komposisi Fisikokimia Tepung Ubi Kayu dan Mocaf dari Tiga Genotipe Ubi Kayu Hasil Pemuliaan Nafilawati wa ode; Emmy Darmawati; Sutrisno Suro Mardjan; Nurul Khumaida
Jurnal Keteknikan Pertanian Vol. 8 No. 3 (2020): Jurnal Keteknikan Pertanian
Publisher : PERTETA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19028/jtep.08.3.97-104

Abstract

Faktor penting yang mempengaruhi karakteristik fisikokimia tepung adalah bahan baku tepung, genotipe/varietas serta proses pengolahan dalam pembuatan tepung. Penelitian ini bertujuan untuk mengevaluasi komposisi fisikokimia tepung ubi kayu dan mocaf dari tiga genotipe ubi kayu yaitu GX, G053 dan G390 yang merupakan hasil pemuliaan tim Croop Improvement IPB. Evaluasi komposisi fisikokimia dilakukan dengan menganalisis kadar air, karbohidrat, protein, lemak, abu, kadar HCN, rendemen dan derajat putih. Berdasarkan hasil analisis diketahui bahwa setiap genotipe ubi kayu memiliki komposisi fisikokimia yang berbeda-beda dipengaruhi oleh jenis genotipe dan proses pengolahannya. Komposisi fisikokimia tepung ubi kayu dari genotipe G053 memiliki keunggulan rendemen (26.10%±0.01), karbohidrat (89.11%±0.015), protein (2.63%±0.014), derajat putih tertinggi (96.89%±0.02) serta kadar air terendah (6.73%±0.004). Tepung dari genotipe G390 memiliki keunggulan kadar abu (0.74%±0.015) dan lemak (0.59%±0.005) terendah. Tepung GX memiliki keunggulan HCN terendah (0.40 ppm±0.198). Komposisi fisikokimia mocaf dari genotipe G053 memiliki keunggulan yaitu karbohidrat, derajat putih tertinggi (98.44%±0.02) dan kadar air terendah (6.65%±0.004). Mocaf dari genotipe G390 memiliki protein (3.01%±0.018) tertinggi kadar abu (0.28%±0.002) dan lemak terendah (0.57%±0.004). Mocaf dari genotipe GX memiliki keunggulan dengan kadar rendemen tertinggi (28.57%±0.08) dan HCN terendah (0.20 ppm±0.10).
Produksi Enzim Lakase Oleh jamur Trichoderma Asperellum Lbkurcci Dalam Bioreactor Tray Menggunakan Variasi Ukuran Substrat Jerami Padi Dan Induser CuSO4 Pada Fermentasi Kultur Padat Gustina Gustina; Sri Helianty; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production and lignin degradation in pulp. There are diverse sources of laccase producing like fungi, plants and bacteria. In this research, the production of lacase enzyme using Trichoderma asperellum LBKURCC1 with bioreactor tray using solid state fermentation (SSF) method with rice straw substrate. The purpose of this study was to determine the effect of rice straw size and the addition of CuSO4 concentration to the highest production of lacase enzyme by Trichoderma asperellum LBKURCC1. Fermentation is carried out with variation of time 5, 6, 7, 8, 9 and 10 days with fermentation temperature ± 30ºC, substrate size ± 0.5 cm, 1.5 cm, and 3.0 cm with substrate thickness at tray ie 3 cm acetate buffer solution pH 5,5 and addition of CuSO4 0.5 g/l. Variations for the addition of CuSO4 concentration 0 g/l, 0.50 g/l and 1 g/l with substrate thickness on tray were 3 cm, size of rice straw 0.5 cm and acetate buffer solution pH 5.5. Small size can provide the highest value and the added concentration of CuSO4 given can increase the activity of the resulting lacase enzyme. However, too high CuSO4 concentrations result in decreased lacase enzyme activity. The results showed that the highest lacase enzyme activity was obtained on the size of rice straw ie 0.5 cm and 7 days of fermentation time with an average of 19.27 U/L and the highest lacase enzyme activity was obtained on CuSO4 0.50 g/l and fermentation time 7 day with an average of 19.27 U/L.Keywords: Inducer, Laccase, Rice straw, Solid state fermentation, Trichoderma asperellum.
Analysis of Variance, Heritability, Correlation and Selection Character of M1 V3 Generation Cassava (Manihot esculenta Crantz) Mutants Rahmi Henda Yani; Nurul Khumaida; Sintho Wahyuning Ardie; Muhamad Syukur
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.844

Abstract

Information about genetic variability and correlation between qualitative character and yield are important to support a selection program. The objective of this research was to determine genetic variability, heritability, and path analysis of M1 V3 cassava mutants’ characters. This research was conducted at Bogor Agricultural University Experimental Field Research from May 2014 to May 2015. This research used 32 mutants from five cassava parent lines which were Malang-4 and Adira-4 (national varieties), UJ-5 (Introduction variety from Thailand), and two local genotypes from Halmahera which were Jame-jame and Ratim. The results showed that gamma ray irradiation increased variability from five cassava genotypes. Characters that had high heritability were length of leaf lobe, lengthof petiole, stem diameter, and the height of plant. The path correlation analysis showed that number of tubers, number of economic tuber (> 20 cm), height to first branchingand stem diameter had direct correlation with tuber mass per plant. The characters can be used for the selection of M1 V4 generation.
ADAPTABILITY OF WHITE JABON (Anthocephalus cadamba MIQ.) SEEDLING FROM 12 POPULATIONS TO DROUGHT AND WATERLOGGING Dede J. Sudrajat; Iskandar Z. Siregar; Nurul Khumaida; Ulfah J. Siregar; Irdika Mansur
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.455

Abstract

The study was carried out for investigation of the adaptability of white jabon seedlings from 12 populations to drought and water logging stresses in a controlled green house. The results showed that the adaptive responses of white jabon seedling to drought and waterlogging stresses were affected by genotype (population). The drought and waterlogging stresses significantly inhibited plant growth, biomass accumulation and allocation, leaf area, also decreased chlorophyll content, increased carotenoids contents, and accumulated free proline. Relative water content and specific leaf area tended to be higher in waterlogging and declined in drought stresses.  The result clearly indicated that white jabon seedlings were more adaptive to waterlogging than to drought stresses. Moreover, there were different responses to drought and waterlogging stresses between the twelve populations. Kampar, Gowa, Kuala Kencana and OKI populations exhibited higher growth performance and stress tolerance index to be adapted to waterlogging stress, while Gowa, Pomalaa and Kampar populations had relatively better growth performance in the drought stress.
PENGGUNAAN FILTRAT Ralstonia solanacearum DALAM SELEKSI KALUS IN VITRO UNTUK KETAHANAN JAHE TERHADAP PENYAKIT LAYU BAKTERI MEYNARTI SARI DEWI IBRAHIM; OTIH ROSTIANA; NURUL KHUMAIDA; SUPRIADI SUPRIADI
Jurnal Penelitian Tanaman Industri Vol 16, No 2 (2010): Juni 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v16n2.2010.49-55

Abstract

ABSTRAKPenyakit layu bakteri yang disebabkan oleh Ralstonia solanacearummerupakan kendala utama budidaya jahe, yang menyebabkan kehilanganhasil lebih dari 90%. Upaya pengendalian yang dilakukan belum optimal,karena tidak tersedianya varietas jahe tahan patogen tersebut. Kendalautama untuk memperoleh varietas jahe yang tahan adalah terbatasnyasumber gen ketahanan dan adanya hambatan fisiologis pada prosespersilangan jahe karena sifat inkompatibilitas sendiri, serta rendahnyafertilitas polen menyebabkan persilangan jahe secara konvensional sulitdilakukan. Seleksi in vitro menggunakan medium selektif yangmengandung filtrat patogen merupakan salah satu metode inkonvensionaluntuk meningkatkan ketahanan tanaman. Penelitian ini dilakukan diLaboratorium Kultur Jaringan dan Laboratorium Penyakit Balai PenelitianTanaman Obat dan Aromatik (Balittro) dari bulan April 2008 sa,mpaiOktober 2008 dengan tujuan untuk mengetahui tingkat ketahanan jahepada stadia kalus terhadap filtrat R. solanacearum dan memperolehkonsentrasi filtrat yang tepat sehingga diperoleh varian kalus baru tahanterhadap filtrat patogen tersebut. Kalus embriogenik jahe putih besar asaleksplan meristem berumur 8 minggu, diseleksi selama 3 minggu di dalammedium proliferasi (MS + 3% manitol tanpa zat pengatur tumbuh),mengandung filtrat R. solanacearum. Seleksi bertingkat dilakukan denganmengaplikasikan filtrat R. solanacearum pada konsentrasi berbeda, yaitu:0; 0,1; 0,2; 0,3; 0,4; 0,5; 1; 2; 3; 4; dan 5%, pada tahap pertama. Padaseleksi tahap kedua, kalus disubkultur ke dalam media yang sama dengankonsentrasi filtrat dinaikkan 10 kali dari konsentrasi awal. Penelitianmenggunakan rancangan acak lengkap, diulang 10 kali. Hasil penelitianmemperlihatkan penggunaan filtrat R. solanacearum di dalam mediumkultur in vitro jahe pada seleksi tahap pertama dan kedua menyebabkanterjadinya perubahan warna kalus dari putih kekuningan menjadi kuningkecoklatan dan coklat kehitaman. Berat dan diameter kalus, jumlahembrio globular serta embrio torpedo berkurang secara nyata setelahperlakuan filtrat, pada seleksi tahap pertama maupun kedua seiring denganbertambah  tingginya  konsentrasi  filtrat.  Konsentrasi  filtrat  R.solanacearum  yang  mampu  menginduksi  dan  menyeleksi  kalusembriogenik berkisar antara 0,3 - 2% dari volume medium seleksi kaluspada seleksi tahap 1 dan 3 - 20% pada seleksi tahap 2.Kata kunci : Zingiber officinale Rosc., kalus, seleksi in vitro,ketahanan, filtrat R. solanacearumABSTRACTThe use of R. solanacearum filtrate in callus selection ofin vitro for ginger resistance to bacterial wilt diseaseBacterial wilt disease caused by Ralstonia solanacearum is the mainconstraint in ginger cultivation. It often causes significant yield loss ofmore than 90%. Various controlling techniques are not able to overcomethe disease, due to unavailability of resistant ginger cultivar. Limitation inobtaining resistant ginger variety is caused by several factors includingthe lack of resistant gene, physiological barrier due to the selfincompatibility, and low pollen fertility, these cause difficulty inconventional cross breeding. Therefore, genetic variability enhancementhas to be carried out unconventionally, to obtain ginger variety resistant tothe disease. In vitro selection using a selective medium containing filtrateof the pathogen is one of the potential unconventional method to improveginger plant resistance. The study was conducted at Meristem Culture andPlant Disease Laboratories of IMACRI from April to October 2008 aimingat determining the level of resistant ginger on stage of calli to the filtrateof R. solanacearum and to obtain an appropriate concentration of thefiltrate which induced calli variants resistant to the filtrate. Large whiteginger embryogenic calli meristems of 8 weeks old were selected for 3weeks in proliferation medium (MS + 3% mannitol without growthregulators), containing filtrate of R. solanacearum. For that purpose, twostages of in vitro selection were performed by applying differentconcentrations of R. solanacearum filtrate e.g; 0; 0.1; 0.2; 0.3; 0.4; 0.5; 1;2; 3; 4; and 5% at the first stage selection. Those concentrations were thenmultiplied 10 times at the second stage selection. Experiments werearranged in completely randomized design with 10 replicates. Resultsshowed that the use of R. solanacearum filtrate as selection agent in gingerin vitro culture medium has caused changes in calli color from theyellowish white into the blackish brown. In addition, increase of R.solanacearum filtrate concentration at the 1 st and 2 nd selection stages wasin line with the decreased of the calli weight and diameter, as well asnumber of globular and torpedo embryo. The concentration of R.solanacearum filtrate applied at 0.3 to 2% in the 1 st selection followed by3 to 20% in the 2 nd  selection induced resistant embryogenic calli of ginger.Key words : Zingiber officinale Rosc., calli, in vitro selection,resistance, R. solanacearum filtrate
PENGARUH UMUR EKSPLAN TERHADAP KEBERHASILAN PEMBENTUKAN KALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE (Zingiber officinale Rosc) MEYNARTI SARI DEWI IBRAHIM; OTIH ROSTIANA; NURUL KHUMAIDA
Jurnal Penelitian Tanaman Industri Vol 16, No 1 (2010): Maret 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v16n1.2010.37-42

Abstract

ABSTRAKKendala dalam pengembangan jahe di Indonesia adalah terbatasnyabenih bermutu. Secara konvensional, budidaya jahe dilakukan denganmenggunakan bibit dari potongan rimpang. Dengan cara ini diperlukanbibit dalam jumlah yang banyak, antara 2-3 t/ha untuk jahe yang dipanentua dan 5-6 t/ha untuk yang dipanen muda. Kendala lain adalah penyakittular benih layu bakteri yang disebabkan oleh Ralstonia solanacearum.Salah satu upaya yang dapat dilakukan untuk mendapatkan benih jahebebas penyakit adalah perbanyakan melalui kultur jaringan. Penelitianbertujuan untuk mengkaji sumber eksplan dari tingkat umur panenrimpang yang berbeda terhadap kapasitas pembentukan kalus embriogenikpada kultur meristem jahe putih besar. Penelitian dilakukan di BalaiPenelitian Tanaman Obat dan Aromatik dari September 2007 sampaiMaret 2008, menggunakan rancangan acak lengkap dengan 20 kaliulangan. Bahan tanaman yang digunakan adalah meristem jahe putih besaryang diambil dari rimpang panen muda dan tua. Peubah yang diamatimeliputi: histologi jaringan, persentase kalus embriogenik yang terbentuk,bobot segar kalus, diameter kalus, dan morfologi kalus. Hasil penelitianmenunjukkan adanya daerah meristematik pada sayatan eksplan meristemjahe putih besar ukuran ± 0,25 cm. Persentase kalus embriogenik (92,1%)dan diameter kalus (0,59 mm) dari rimpang yang dipanen tua lebih tinggidari yang dipanen muda. Berat kalus (1,18 g) dan jumlah embrio somatikglobular (29,34) asal eksplan panen tua nyata lebih tinggi dari yangdipanen muda. Kalus embriogenik yang berasal dari eksplan rimpang yangdipanen tua mampu berkembang membentuk embrio somatik danberkecambah menghasilkan planlet normal.Kata kunci : Zingiber officinale Rosc., umur rimpang, kalus embriogenik,embriogenesis somatikABSTRACTEffect of explants age on the success of embryogenic calliformation in meristem culture of ginger (Zingiberofficinale Rosc.)Constraint in ginger cultivation in Indonesia is the limited qualityof planting materials. In conventional cultivation, planting materials weretaken from a piece of rhizomes. By this technique, significant amount ofplanting materials is required, between 2-3 tons/ha for fully harvested and5-6 tons/ha for young harvested rhizomes. Another serious constraint isbacterial wilt disease infection caused by Ralstonia solanacearum. Effortfor obtaining free disease planting materials could be performed throughtissue culture mass propagation. In this study, different ages of rhizome asexplants sources was evaluated for their capacity in embryogenic calliformation on the meristem culture of ginger. The experiment wasconducted in Indonesian Medicinal and Aromatic Crops Research Institutefrom September 2007 to March 2008, using a completely random designwith 20 replicates. Plant material used was white ginger meristem takenfrom the fully and young harvested rhizomes. The observed variables wereexplant histology, percentage embryogenic calli formation (%), freshweight of calli, calli diameter, number of globular embryo, and callimorphology. The results showed a meristematic region at the incisionexplant big-white ginger meristem ± 0.25 cm in size. Percentage ofembryogenic calli formation from the fully harvested rhizome-explant(92.1%) and calli diameter (0.59 mm) were higher than that of the youngerone. Calli weight (1.18 g) and number of globular somatic embryos(29.34) from fully harvested rhizome-explants were significantly higherthan that of the younger one. Embriogenic calli derived from the oldharvested rhizome explants was able to grow well to form somaticembryos and then germinate to produce normal plantlet.Key words : Zingiber officinale Rosc, age of rhizome, embriogeniccalli, somatic embryogenesis
Co-Authors , Kisman , Sukendah , Witjaksono . Wusnah A. Sofwan Achmad . ADI SETIADI Agus Purwito Agustina, Ferra Anggita AHMAD JUNAEDI Ahmad Rifqi Fauzi Ali Djamhuri Almia Permata Putri Amril Aman Andi Dahliaty Aniversari Apriana Aniversari Apriana Aniversari Apriana Ardana, I. N. Kutha Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Aris Purwanto Arrin Rosmala Atmitri Sisharmini Atmitri Sisharmini Atmitri Sisharmini Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti Bintang, dan Maria Buang Abdullah Cakranegara, Pandu Adi Cucu Sukmana, Cucu Danar Dono Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat Dede Kurniadi DEWI SARTIAMI Dewi Sukma Dewi Sukma Didy Sopandie Diny Dinarti Dwi Pratiwi Edi Santosa Efendi, Darda Emmy Darmawati Enny Sudarmonowati Evi Fitriany Faqih Udin Firdausya, Andina Fabrini Freddy Tua Musa Panggabean Gustaaf A Wattimena, Gustaaf A Gustaaf A. Wattimena Gustaaf Adolf Wattimena Gustaaf Adolf Wattimena HADIPOENTYANTI, ENDANG Hartati, RR. Sri Heldiyanti, Rina Hendri Hermawan Adinugraha HUSNI, ALI I Ismayani I MADE ARTIKA Ida Duma Riris Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Roostika Indrastuti Apri Rumanti Irdika Mansur Irfan MARTIANSYAH Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Jalaluddin Jalaluddin Kartika Ning Tyas Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko La Muhuria Lefin Kafindra Lestari, Risza Putri Lisnawaty Simatupang Lola Adres Yanti Lukman Hakim M Murtadha M. Amir M. Asyabul Zikki Maemonah, Maemonah MARIA BINTANG Marini Damanik Mariska, Ika Maryati Evivani Doloksaribu MEYNARTI SARI DEWI IBRAHIM MEYNARTI SARI DEWI IBRAHIM Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Muhammad Irfan Habibi MUHAMMAD SYUKUR Mulyatno, Mulyatno Munif Ghulamahdi N. Sri i Hartati Nafilawati wa ode Nata Suharta Natalini Nova Kristina Natalini Nova Kristina Nataniel Tandirogang Nugroho, Candra Catur Nura ,, Nura Nurbaiti Nurbaiti Nurhasybi Nurhasybi Nurul Fatimah Nurul Fauziah O K Sofyan OTIH ROSTIANA OTIH ROSTIANA Pienyani Rosawanti Rahmi Henda Yani Rasyad, M. Ashadi Reny Herawati Rina Hapsari Wening, Rina Hapsari Rinda Cahyana Riyanti Catrina H S Riza Arief PUTRANTO Rizkiannur Putri, Amalia Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rozanna Dewi Ryan Budi Setiawan Sadewi Maharani Sadewi Maharani, Sadewi Saepudin, Adam Safitri Safitri Saronom S ilaban Sarwono Hardjowigeno Shafwan Fahmi Silawati, Tutisiana Silawati Tutisiana Sintho Wahyuning Ardie Siregar, Ulfah J Siswati, Leni Siti Aisyah Siti Kurniawati Slamet Budijanto Slamet Susanto Sobir Sobir Sri Enny Triwidiastuti Sri Helianty Sry Rahmadani Subekti, Isnani Sudarsono Sudarsono Sudarsono Suhesti, Sri Sukma, Dewi Sulastri Sulastri Sulhatun Sulhatun SUPRIADI SUPRIADI Suryati Suryati Suryo Wiyono Sutalaksana, Iftikar Z Sutrisno Mardjan Sutrisno, Sutrisno Suyanti Kasimin Syamsul Bahri Syamsul Bahri Tarigan, Asmara Iriani Tengku Mia Rahmiati Teti Arabia Thifany, Ariny Jihan Tri Handayani Tri Joko Santoso Tri Lestari Mardiningsih Tri Lestari Mardiningsih, Tri Lestari Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Trismi Ristyowati Ulfah J. Siregar Ulfah Juniarti Siregar Ummu Kalsum Wahyuning Ardie, dan Sintho Waizul Fahri Purba Waras Nurcholis Warid Warid Wattimena, and Gustaaf Adolf WATTIMENA, G. A. Wening Enggarini Wening Enggarini Widiatmaka Widodo , Widowati, Sartika Widya Hartati, Widya Wijayanti, Mustika Eka Willy Bayuardi Suwarno Yudiansyah Yudiansyah Yulia, Endah Yulia Yundari, Yundari Yunita, Rossa Zuli Nuraeni