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Journal : Frontier Advances in Applied Science and Engineering

Study of Inhibition DNA Polymerase by Phenolic Compounds in Traditional Food Spices on the Polymerase Chain Reaction Process Fadila Abna Swari; Suharti, Suharti; Evi Susanti; Eli Hendrik Sanjaya; Sumari, Sumari
Frontier Advances in Applied Science and Engineering Vol. 1 No. 1 (2023)
Publisher : Tinta Emas Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59535/faase.v1i1.108

Abstract

Polymerase Chain Reaction (PCR) is a repeated cycle process including denaturation, annealing and extension using the DNA polymerase enzyme. This identification method is based on specific DNA which increases the number of DNA strands millions of times. The aim of this study was to identify and classify phenolic group compounds in traditional food spices that have the potential to inhibit DNA polymerase and their mechanism of action in the PCR process. The observation made was the Literature Review study as an analytical test method regarding the study of DNA polymerase inhibition by phenolic group compounds in traditional food spices in the PCR process. The results of this study were analyzed from several appropriate literature studies showing that most of the spices in traditional foods contain secondary metabolites of phenolic group compounds. These compounds have been shown to inhibit DNA polymerase in the PCR process, one of the mechanisms is to bind to the active site of DNA polymerase which causes decreased activity and deactivates DNA polymerase. Moreover, it can degrade and denaturate DNA polymerase. This makes it difficult to identify haram substances in processed food products using PCR.
Co-Authors Abrilia, Putri Riza Ahmad Munjin Nasih Alifah Novitasari Alvionita, Mieke Aman Santoso Amara, Aulia Anggraini, Dyas Anie Yulistyorini Annifah, Riri Uswatun Anugrah Ricky Wijaya Ardyansyah, Ananta Armaini, Armaini Asep Kadarohman Ashfiya, Putri Sayyida Aziz, Hafiz Aji Billah, Egi Nursari Budiman Anwar Burhanuddin Ronggopuro Deni Ainur Rokhim Dermawan Afandy Dewi, Ratna Kumala Edy, Duwi Leksono Evi Susanti Evy Damayanthi Fadhil Md Din, Mohd Fadila Abna Swari Febriana, Silvia Fitri Khoerunnisa Hakiki, A.Riyan Rahman Hayuni Retno Widarti Hendrawan Hendrawan Hengky Pamungkas, Yahya Hidayati, Elvi Hidiyah, Tabita May Hong Chen Iasya, Yurin Karunia Apsha Albaina Ida Hamidah Iklima, Ilma Kanzu Imam Alfianto Isma Yanti Vitarisma Jacky Anggara Nenohai Kerinandhila, Kerinandhila Lakapu, Erlita Laurent Octaviana Li, Yu-You Lilik Eka Radiati Listyo Yudha Irawan Maharani, Brigitta Stacia Muh Ade Artasasta Muhammad Ali Murtadho Muhammad Reza Pahlevi Muhammad Roy Asrori Mujahidah, Amiroh Nabilah Muntholib Munzil Nurhayati, Mita Parlan . Prastiwi, Mellinia Regina Putri, Nanda Regita Qodriyah, Nur Romadhona Lailatul Retno Wulandari Riska N.M., Nofita RR. Ella Evrita Hestiandari Sarif Hidayat Shinta Yuliana Shrestha, Rajendra Prasad Sinta Yuliana Siti Mutmainah Siti Sendari Sitoresmi Prabaningtyas Sofinila, Mida Fitri Agasty Suharti Suharti Sumari Sumari Syamsul Bachri Taupik Hidayat, Taupik Wahab, Roswanira Abdul Wijayanti, Chandra Yahmin Yahmin Yaya Sonjaya Yudhi Utomo YULIANA, SHINTA Zarin Nafasari Zelen S. Minata