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Analysis of Starch, Chlorophyll, and Antioxidant Content of Cardamom (Amomum cardamomum) Leaves from Various Accessions Lelly Aulia Damarhati; Sriwongo, Stephanie; Taureza, Aulia; Mulyati, Teti; Liwanda, Novian; Nurcholis, Waras
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.44781

Abstract

Cardamom (Amomum cardamomum) is a herbal plant with various phytochemical compounds such as phenols, starch, tannins, terpenoids, flavonoids, and antioxidants. Cardamom of the same type but originating from different regions can produce variations in the content of metabolites. This study aimed to select cardamom accession containing high starch, chlorophyll, and antioxidant capacity. The research was conducted at Biopharmaca Experimental Garden, Cikabayan IPB University from March to August 2022 using four cardamom accessions, i.e., Bogor Hijau, Bogor Merah, Ciamis and Sukabumi. Three leaves were taken for each accession, with three replications. The leaves taken were numbers 5, 6, and 7 counted from the tops of the cardamom plant. The methods used in this study were the phenol-sulfuric acid method, the DMSO method, the DPPH method, and the CUPRAC method. The results showed that the highest starch content was produced by the cardamom of Bogor Merah accession, with an average of 3.63 g (100 g)-1 FW. The highest total chlorophyll content was found in the cardamom of Bogor Hijau accession, with an average of 1.9 mg g-1 FW. At the same time, the highest antioxidant capacity using the DPPH method was produced by the cardamom of the Sukabumi accession with an average of 1.28 μmol TE g-1 FW. In comparison, the highest antioxidant capacity of the CUPRAC method was produced by the cardamom of the Bogor Hijau accession with an average of 5.71 µmol TE g-1 FW. Thus, it is recommended to use Bogor Merah dan Bogor Hijau accession for further evaluation. Keywords: accession, antioxidant activity, cardamom leaves, chlorophyll, starch
Improvement of iron content, total phenolic, and antioxidant activity of green spinach (Amaranthus tricolor L.) with maggot fertilizer Andrianto, Dimas; Nurcholis, Waras; Ghifari, Muhammad Nabil
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.50063

Abstract

Green spinach (Amaranthus tricolor L.) is a plant that contains various vitamins, such as vitamins A, B, and C, as well as protein and fiber. Green spinach contains high iron. Besides that, spinach also contains secondary metabolites such as flavonoids, antioxidants, and phenolics, which are beneficial for degradative diseases. This study aimed to evaluate the impact of plant height, iron content, total phenolic content, and antioxidant activity in spinach plants using a 70% ethanol solvent. The samples were extracted through a maceration method in a microwave for 3 minutes, followed by filtration to obtain the test sample filtrate. Iron content was determined using the ICP-OES method, total phenolic content (TPC) was measured with the Folin-Ciocalteu method, and antioxidant activity was assessed using the DPPH method. The findings revealed that treatment 2 (2 parts maggot fertilizer to 8 parts soil) had the highest iron content and antioxidant activity, whereas treatment 1 (1 part maggot fertilizer to 9 parts soil) showed the greatest plant growth and phenolic content. Keywords: allelochemical; bioherbicide; inhibitory effect; weed control
Optimization of Extraction Conditions to Enhance Polyphenol Content and Antioxidant Activity İn Orthosiphon Aristatus Leaves Febriyanto, Dimas; Setyawati, Inda; Ambarsari, Laksmi; Nurcholis, Waras
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.16820

Abstract

ABSTRACT. Orthosiphon aristatus (Java tea) is a medicinal plant known for its high polyphenol content and antioxidant properties. Unlike previous studies that focused solely on solvent type or temperature, this study simultaneously optimizes temperature and pH extraction parameters to achieve maximal bioactive compound recovery. This study aimed to optimize extraction parameters—temperature and pH—to enhance polyphenol yield and antioxidant activity in methanolic extracts of Java tea leaves. Samples were extracted at temperatures ranging from 30 °C to 80 °C (pH 7) and pH levels from 2 to 6 (at room temperature). Total phenolic content (TPC) and total flavonoid content (TFC) were determined to quantify polyphenol levels, and antioxidant activity was evaluated using four complementary in vitro assays: DPPH, ABTS, FRAP, and CUPRAC. The highest TPC (9.68 mg GAE/g DW) and TFC (6.53 mg QE/g DW) were observed at 70 °C and 80 °C, respectively. Maximum antioxidant activities were observed at 70–80 °C and pH 2–3, with peak values of 102.71 µmol TE/g DW (ABTS), 101.9 µmol TE/g DW (FRAP), and 56.12 µmol TE/g DW (CUPRAC). A strong correlation was found between phenolic content and antioxidant capacity. These findings highlight the critical role of extraction conditions in maximizing the biofunctional potential of O. aristatus and support its application in pharmaceutical and nutraceutical formulations. The study provides a scientific basis for developing standardized extraction protocols to improve functional ingredient consistency in herbal product development. Keywords: Antioxidant activity, extraction optimization, Orthosiphon aristatus, pH effect, temperature
Strengthening Community Health Resilience: Hypertension and Diabetes Education, Local Herbal Drink Training, and Natural Handwash Production in Sukabumi Wulan Tri Wahyuni; Ulfiatun Nisa; Irmanida Batubara; Trivadila Wahyuni; Eka Widya Nugraha; Yusuf Ryadi; Waras Nurcholis
Indonesian Journal of Community Engagement Vol. 3 No. 1 (2026): (January) Indonesian Journal of Community Engagement
Publisher : PT. Altaf Publishing Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70895/ijce.v3i1.91

Abstract

This community engagement program aimed to strengthen health resilience among women in Sukabumi by enhancing their understanding of hypertension and diabetes, while also building practical skills in preparing local herbal beverages and natural handwash products. A total of twenty-five PKK members from Karangtengah Village participated in structured educational sessions delivered by medical lecturers, baseline screening of blood pressure and blood glucose levels, and hands-on training utilizing locally available resources. Participants’ knowledge was assessed using matched pre- and post-tests, while behavioural adoption was evaluated through post-intervention self-reports. The screening results revealed that a substantial proportion of participants exhibited elevated blood pressure and/or blood glucose levels, highlighting the relevance and urgency of the program. Significant improvements in knowledge were observed across all assessed domains, including risk factors, early symptoms, preventive lifestyle practices, and recognition of locally available herbal ingredients. All knowledge gains were statistically significant, indicating effective health information transfer. The practical training component further enhanced participants’ confidence and self-efficacy, as reflected by a high intention to adopt the recommended practices in daily life. Overall, the program demonstrated that community-based, participatory health education combined with culturally appropriate practical training can effectively enhance health knowledge, support positive behavioural change, and promote sustainable household health practices in rural communities.
Optimization of Black Orchid (Coelogyne pandurata Lindl.) Extraction Using Simplex-Centroid Design for Total Phenolics, Flavonoids, and Antioxidants Pebra Heriansyah; Novian Liwanda; Waras Nurcholis
Jurnal Jamu Indonesia Vol. 11 No. 1 (2026): January–February 2026
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i1.457

Abstract

Coelogyne pandurata Lindl., a black orchid endemic to Kalimantan, contains valuable antioxidant compounds. This study optimized its extraction using a Simplex-Centroid Mixture Design with ethanol, methanol, and acetic acid as solvents. Responses evaluated were total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity (DPPH). The highest TPC was obtained with 100% ethanol (2.52 mg GAE/g), TFC with equal parts of all solvents (6.41 mg QE/g), and antioxidant activity with 100% methanol (10.33 µmol TE/g). Ethanol yielded the optimal extraction condition, with verification values of 2.27 mg GAE/g (TPC), 3.42 mg QE/g (TFC), and 10.22 µmol TE/g (DPPH), and a desirability of 0.739. Although regression models were not statistically significant (p > 0.05), the results provide preliminary insights into solvent efficiency for extracting bioactive compounds. Further studies with expanded experimental designs and validation are required to enhance model accuracy and extraction reliability.
Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia NURUL KHUMAIDA; MUHAMAD SYUKUR; MARIA BINTANG; WARAS NURCHOLIS
Biodiversitas Journal of Biological Diversity Vol. 20 No. 3 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200306

Abstract

Abstract. Khumaida N, Syukur M, Bintang M, Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20: 656-663. Curcuma aeruginosa is a rhizomatous medicinal plant with beneficial pharmacological activities. The aim of this work was to analyze the agro-morphological, extract yield, and phenolic content of ten C. aeruginosa accessions which were collected from different locations in Indonesia. Cultivation was carried out in the open field in West Java of Indonesia using a completely randomized design. Qualitative and quantitative parameters were used to investigate agro-morphological traits. Total phenolic and total flavonoids contents were determined in ethanol extracts of samples. The plants were phenotypically diverse, in which there were significant variations among the ten C. aeruginosa accessions in number of leaves, plant height, number of shoots, fresh weight of rhizome, and dry weight of rhizome characters. Variability in the total phenolic and total flavonoid contents ranged from 29.08-46.92 mg GAE/g, and 21.31-33.81 mg QE/g, respectively. Six accessions had high phenolic content and extract yield. Therefore, these accessions could be utilized for commercial scale and also showed a high potency for medicinal plant breeding programs.
Volatile compound profiles and cytotoxicity in essential oils from rhizome of Curcuma aeruginosa and Curcuma zanthorrhiza RIZKI FITRIA; DJAROT SASONGKO HAMI SENO; BAMBANG PONTJO PRIOSOERYANTO; HARTANTI; Waras Nurcholis
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201024

Abstract

Abstract. Fitria R, Seno DSH, Priosoeryanto BP, Hartanti, Nurcholis W. 2019. Volatile compound profiles and cytotoxicity in essential oils from rhizome of Curcuma aeruginosa and Curcuma zanthorrhiza. Biodiversitas 20: 2943-2948. Cancer becomes one of the uncontagious diseases that occupy the top position in the cause of human death under 70 years in the world. Breast cancer is the most widely experienced cancer by women. The aim of this study was to obtain cytotoxic activity essential oil of Curcuma aeruginosa and Curcuma zanthorrhiza as antiproliferative substance of cancer cells and to analyze volatile profile of C. aeruginosa and C. zanthorrhiza essential oils. The essential oils were obtained from rhizomes of C. aeruginosa and C. zanthorrhiza by using hydrodistillation method. Volatile compositions of essential oils were analyzed using gas chromatography and mass spectrophotometry (GC/MS). The cytotoxic activities of the essential oil samples were evaluated using brine shrimp lethality test and against MCF-7 (Michigan Cancer Foundation-7) and Vero cells. The essential oils of C. aeruginosa, and C. zanthorrhiza showed cytotoxic activities to brine shrimp and MCF-7. Cytotoxic activity presented in essential oil from C. aeruginosa and C. zanthorrhiza showed lower activity compared with Doxorubicin. The major compounds of C. aeruginosa were tropolone (18.1%), eucalyptol (17.9%), and curcumol (5.7%), whereas C. zanthorrhiza were xanthorrhizol (26.8%), b-curcumene (17.0%), ar-curcumene (15.0%), and germacrone (5.4%).
The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds SYARIFAH IIS AISYAH; IZZATUL MUHALLILIN; DEWI SUKMA; Waras Nurcholis
Biodiversitas Journal of Biological Diversity Vol. 20 No. 12 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201240

Abstract

Abstract. Aisyah SI, Muhallilin I, Sukma D, Nurcholis W. 2019. The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds. Biodiversitas 20: 3766-3771. The present work investigated the agro-morphological changes in Celosia cristata L. seeds exposed to gamma irradiations. This study also conducted phytochemical screening to screened secondary metabolite change in C. cristata. C. cristata seeds were irradiated with acute radiation at doses of 470, 480, and 490 Gy and were planted until M1 generation. Recurrent irradiation was done on M1 seeds at a dose of 250 Gy. The agro-morphological and phytochemical diversity was performed in M2 generation. The highest difference characters in acute irradiated plants were found on plant height, number of branches, and flower length, while the plant diameter, flower width, and number of flowers were found the highest in recurrent irradiation. Agro-morphological important changes in recurrent irradiated plants were decreased plant height and increased the number of flowers that compared with acute irradiated and control plants. The qualitative phytochemical showed no difference between acute and recurrent irradiated plants. It can be concluded that the recurrent irradiated changes the morphological valuable of C. cristata, while the phytochemical is needed further research.
Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia JANSON CALVINDI; MUHAMAD SYUKUR; Waras Nurcholis
Biodiversitas Journal of Biological Diversity Vol. 21 No. 6 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210612

Abstract

Abstract. Calvindi J, Syukur M, Nurcholis W. 2020. Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia. Biodiversitas 21: 2420-2424. Winged bean, Psophocarpus tetragonolobus (L) DC, is described as having antioxidant properties. This work evaluated the biochemical and antioxidant characteristics of the P. tetragonolobus genotypes. Twelve-winged bean genotypes were calculated for total phenolic content (TPC) and total flavonoid content (TFC) of biochemical characters and antioxidant properties by using four methods: 2,2-diphenyl picrylhydrazyl (DPPH), cupric reducing antioxidant power (CUPRAC), ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC). The plant material was grown in the same location conditions in Indonesia. The total phenolic content varied from 154.6 to 161.5 mg GAE/ 100 g, and flavonoid ranged from 105.2 to 112.4 mg QE/ 100 g fresh weight. The antioxidant capacities were 30.6 - 47.0, 140.4 - 167.6, 66.9 - 170.8, and 28.0 - 52.4 µmol TE/ 100 g fresh weight as calculated by the DPPH, TEAC, FRAP, and CUPRAC assays, respectively. The antioxidant activities were significantly correlated with the polyphenol content of winged bean genotypes fruits. The genotypes TU, L3, H3U, H1P, and TH were recognized higher based on their TPC, TFC, and antioxidant activities, indicating that these genotypes to be promising for further breeding program and commercial purposes.
GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent Waras Nurcholis; NURUL KHUMAIDA; MARIA BINTANG; MUHAMAD SYUKUR
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220313

Abstract

Abstract. Nurcholis W, Khumaida N, Bintang M, Syukur M. 2021. GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent. Biodiversitas 22: 1179-1186. This work aimed to evaluate the bioactive compounds and anticancer activity in rhizome extract of ten Curcuma aeruginosa accessions to explore their pharmacological values. The GC-MS analysis was used to identify bioactive compounds. The cytotoxic activity was performed against MCF-7 (Human breastadeno carcinoma) and Vero cell lines using MTT assay. The GC-MS analysis revealed 71 of the compounds as sesquiterpenes (36), monoterpenes (20), phenolics (5), diterpenes (4), phenanthrene (1), tetrapeptides (1), oxazole (1), triazine (1), piperidine (1), and oxygenated hydrocarbons (1). The isocurcumenol was the most dominant metabolite in ethanol extract of C. aeruginosa rhizome, with the highest produced by KP accession (22.01%) followed MD accession (21.12%). However, camphor and ?-elemene were the metabolites produced by all accessions studied. In the Vero cell line as a normal cell, the cytotoxic activity varied from 13.28% (MD) to 45.17% (PW). Furthermore, the cytotoxic activity ranged from 1.16% (LC) to 49.70% (MD) against the MCF-7 cell line. The highest anticancer activity was produced in MD accessions; thus, it can be used as a source of quality raw materials for the pharmaceutical and food industry. Besides that, it can also be further developed to obtain superior varieties through plant breeding programs.
Co-Authors . TRIVADILA A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA ALDILA WIDYA PUTRI OKTAVIA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza ANNISA ZAHRA Antonius Padua Ratu Antonius Padua Ratu Anyalirria Anyalirria AN’NISA ZAHRO NAFISAH Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra ASMAUL CHUSNA AL ANSHORY Asy Syifa Tickna Maulana AULIA ARDHIAN AYUNINGTYAS Auliya Ilmiawati Awang Maharijaya Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia DARA JULIANA Dara Juliana DEWA NGURAH SUPRAPTA Dewi Sukma DEWI YANNY LENAWATY Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO DINI DINARTY Dita Audia Putri DJAROT SASONGKO HAMI SENO Djarot Sasongko Hami Seno DJAROT SASONGKO HAMI SENO Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Eka Widya Nugraha Enny Rimita Sembiring Erna Subroto Eva Harlina FACHRUR RIZAL MAHENDRA Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Farras Agung Binukajati Febriyanto, Dimas Fina Febrianti FIRA ANANDA Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy GARDA YODI Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning HARTANTI Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi HEDER DJAMALUDIN HIROSHI TAKEMORI Hyakansa Hyakansa I Made Artika I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara IZZATUL MUHALLILIN JANSON CALVINDI Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI LAKSMI AMBARSARI LANI HARDIANY Latifah K Darusman Latifah K Darusman latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa MARIA BINTANG Maria Bintang MARIA BINTANG MARIA BINTANG Maria Magdalena MARLIN MARLIN Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Milanda Fiorella Gultom MINARNI MINARNI MINARNI MINARNI Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Renza Fajriansyah Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak NELLY MARLIANI Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar NOVIAN LIWANDA NOVIAN LIWANDA Novian Liwanda Novian Liwanda Novita Sari NUR IZZAH MEININGRUM NURLELA NURLELA NURUL KHUMAIDA NURUL KHUMAIDA Nurul Khumaida NURUL KOMALA Nurul Komala NYIMAS SHANIA AZIRA UBAI P.Roswiem, Anna Pebra Heriansyah Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah RAIHAN PERMANA PUTRA RAIHAN PERMANA PUTRA Raihan Permana Putra RAISADIBA PUTRI BOVANI Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna RAYANDRA ASYHAR RAYANDRA ASYHAR REGINA AGRITENIA MAYRISCHA SARASWATI Rerenstradika Tizar Terryana RIFAN NURFALAH Riki Riki Rikno Harmoko RINI ANGGI ARISTA Rini Arianti Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria RIZKI FITRIA RONNY YUNIAR GALINGGING Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti SELNIS PRASSISKA Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael SILVIA SANTIKA SITI JAMILAH Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani SURYANI SURYANI Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama TAOPIK RIDWAN Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Ulfiatun Nisa Utami Dyah Safitri Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi YOSHUA SHANDY YUDHA YOSHUA SHANDY YUDHA YOSHUA SHANDY YUDHA Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Yusuf Ryadi Zamany, Nevianti Zulfikar D Sahid ZULFIKAR DAMARALAM SAHID