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All Journal International Journal of Advances in Applied Sciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Hemera Zoa Vegetalika VALENSI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Chemistry Agrin : Jurnal Penelitian Pertanian Justek : Jurnal Sains Dan Teknologi Biosaintifika: Journal of Biology & Biology Education Media Farmasi Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia YARSI Medical Journal Poltekita : Jurnal Ilmu Kesehatan Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Jamu
Waras Nurcholis
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (IPB University), Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Other

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Optimization of Extraction Solvent for Analysis of Total Phenolic and Antioxidant Activity of Guava Leaves (Psidium guajava) using Simplex Centroid Design Lelly Aulia Damarhati; Muhamad Renza Fajriansyah; Anyalirria; Raihan Permana Putra; Novian Liwanda; Waras Nurcholis
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.344

Abstract

Guava (Psidium guajava) is a popular tropical plant widely used as processed food and medicine for various diseases. This plant contains highly bioactive compounds like quercetin, flavonoids, and ferulic acid. The bioactive content can be influenced by the extraction method. However, the solvent used for extraction can also affect the bioactive content. Therefore, this study aims to determine the best solvent or combination of extraction solvents to extract guava leaves based on total phenolic content (TPC) and antioxidant activity using simplex centroid design (SCD). This research used guava leaf samples with various solvent combinations of water, ethanol, and acetone. The determination of phenolic content was determined using the TPC method, while the determination of antioxidants was conducted using the FRAP and ABTS methods. The research results showed that the highest TPC levels were produced by the solvent water : ethanol : acetone. Then the highest levels of FRAP and ABTS antioxidants were produced by the water: ethanol solvent. The solvent optimization solution obtained was using water: ethanol: acetone with a ratio of 0.462:0.436:0.102, respectively.
Editorialboards Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 4 No. 2 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i2.133

Abstract

Puji dan syukur kami panjatkan kepada Tuhan Yang Maha Esa atas rahmat dan hidayahNya, Jurnal Jamu Indonesia Volume 4 No 2 tahun 2019 dapat diterbitkan. Jurnal Jamu Indonesia merupakan media untuk diseminasi hasil penelitian yang berkaitan dengan jamu di Indonesia. Jamu telah dikenal lama penduduk Indonesia sebagai salah satu pengobatan. Berbagai tanaman di Indonesia sangat berpotensi sebagai jamu. Penelitian tanaman obat dari bagian daun, batang, dan rimpang tanaman telah banyak dilakukan dan terus berkembang. Naskah dalam edisi ini membahas analisis ramuan jamu antihipertensi, analisis performa pertumbuhan dan produktivitas daun duduk (Desmodium triquetrum (L.) D.C.) terhadap ketinggian tempat, khasiat antihiperglikemia daun kelor, khasiat antimikroba tanaman paku, dan khasiat jamu antidiabetes secara in vivo pada tikus. Redaksi mengucapkan terima kasih kepada seluruh penulis atas partisipasinya dalam berbagi hasil penelitiannya melalui publikasi di Jurnal Jamu Indonesia. Redaksi juga mengucapkan terima kasih kepada seluruh reviewer atas partisipasinya dalam meningkatkan kualitas tulisan naskah jurnal di edisi ini. Semua hasil karya tersebut akan diarsipkan sebagai dokumentasi berharga demi terjaganya informasi yang dapat menjadi sumber pengembangan ilmu pengetahuan di masa depan. Kontribusi publikasi peneliti-peneliti dan pejuang Jamu Indonesia terus kami harapkan untuk dapat disampaikan di dalam Jurnal Jamu Indonesia ini. Peningkatan kontribusi jumlah dan kualitas tulisan yang telah dan akan dimasukkan akan dapat membantu pengembangan jurnal ini, dan pada saatnya diharapkan juga dapat menjadikan Jurnal Jamu Indonesia menjadi referensi baik secara nasional, maupun internasional. Juni 2019Waras NurcholisWakil Dewan Redaksi
Dynamics of Nutrient Concentrations, Endogenous Hormones, Photosynthetic Capacity, and Phenological Changes in Black Orchid (Coelogyne pandurata Lindl.) from the Vegetative to Generative Phase Heriansyah, Pebra; Aziz, Sandra Arifin; Sukma, Dewi; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.215-234

Abstract

The black orchid (Coelogyne pandurata Lindl.) is one of the native Indonesian orchids from Borneo island. This study analyzed the dynamics of pigments, photosynthetic capacity, endogenous hormones, nutrient concentrations, and phenology across the vegetative to generative stages of the black orchid. The plant materials are one-year-old plants after splitting from the mother plants. Endogenous hormones, nutrient and pigment concentrations, and leaf photosynthetic capacity were measured during vegetative and generative phases. Chlorophyll, anthocyanins, and carotenoids were analyzed using UV-VIS spectrophotometry, Nitrogen (N) was analyzed by the Kjeldahl method, phosphorus (P) and potassium (K) by the Bray 1 method and Flame Photometry, and organic carbon by the Walkley- Black method with titration. The hormone levels were analyzed using HPLC, and photosynthetic capacity was determined using a Li-Cor 6800 system. Phenological changes in leaves, bulbs, and flowers were recorded. Results showed total chlorophyll increased from 1.96 to 2.36 mg/g from the vegetative to the generative stage while carotene slightly declined. Leaf nitrogen rose from 1.54% to 1.70%, bulb nitrogen decreased from 0.67% to 0.45%, whereas the C/N ratio increased from 65.24 to 85.36 from the vegetative to the generative phase. Flower nitrogen was 1.50%, and phosphorus was 0.17%. IAA in leaves decreased from 25.12 to 21.37 ng.g⁻¹ dry weight post-flowering, while gibberellin in bulbs increased from 12.28 to 12.96 ng.g⁻¹ dry weight. Zeatin in bulbs declined from 10.52 to 8.81 ng g⁻¹ dry weight. Photosynthesis peaked at 2.73 μmol. m⁻².s⁻¹ in early generative stages and then declined. Photosynthetic photon flux density and stomatal conductance decreased, reducing water and CO₂ exchange efficiency, while net assimilation and transpiration rates showed no significant changes. These physiological adjustments, including increased chlorophyll levels, changes in nitrogen allocation, and fluctuations in hormone concentrations, reflect the plant’s adaptive strategies to meet higher energy demands during reproductive growth, ensuring efficient resource distribution for flowering.
The Effect of Shade and Harvest Age on Biomass and Patchouli Oil Production in the Lowlands Riti, Dominggus Ngongo; Kurniawati, Ani; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.426-436

Abstract

This study investigated the effects of different shade levels and harvest ages on the growth, biomass yield, and essential oil production of the patchouli (Pogostemon cablin Benth.) cultivar Sidikalang, cultivated in a lowland environment. The experiment was conducted in Bogor from August 2023 to February 2024, using a nested design with three shade treatments (no shade, 55%, and 75%) and three harvest ages (4, 5, and 6 months). The results indicated that 75% shade significantly enhanced vegetative growth, including plant height, number of secondary branches, and leaf production, suggesting that this level of shading is beneficial for patchouli cultivation in lowland areas. However, these effects were not significantly different from those observed under 55% shade. The highest essential oil yields were obtained from patchouli plants grown under 75% shade and harvested at 4 months, as well as from those grown under full sunlight and harvested at 6 months.
TLC Fingerprint Analysis and Evaluation of α-Glucosidase Inhibitory and Free Radical Scavenging Activity of Justicia gendarussa from Different Growth Locations Wulansari, Laela; Wahyuni, Wulan Tri; Nurcholis, Waras; Rafi, Mohamad
Jurnal Kimia Valensi Vol. 11 No. 1 (2025): Jurnal Kimia VALENSI
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.43662

Abstract

Justicia gendarussa is known to have antioxidant and antidiabetic properties, which are related to the composition and concentration of its metabolites and influenced by differences in growth location. This study aims to evaluate changes in metabolite profiles and biological activities, such as the inhibition of α-glucosidase and free radical scavenging in J. gendarussa genotypes from Bogor, Cianjur, and Sukabumi. Changes in metabolite profiles were assessed using thin-layer chromatography (TLC) fingerprint analysis combined with principal component analysis (PCA). Bioautography TLC using DPPH was performed to confirm the presence of antioxidant compounds. In the TLC fingerprint analysis of J. gendarussa, we obtained 14 bands with good resolution. PCA successfully grouped J. gendarussa extracts based on growth location. The percentage of α-glucosidase inhibitory and free radical scavenging activity was significantly different, with the highest percentage of inhibition of α-glucosidase shown by the IIIS genotype (99.23%) and the highest free radical scavenging (91.35%) demonstrated by the IIB genotype. The results of TLC bioautography confirmed the presence of antioxidant compounds in J. gendarussa represented by bands with Rf 0.04, 0.32, and 0.80. This study concludes that growth location differences influence the variations in metabolite profiles, α-glucosidase inhibitory activity, and free radical scavenging activity of J. gendarussa genotypes.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Anyalirria Apipah Aprianti Arianti, Rini Arya Arendra Asy Syifa Tickna Maulana Aulia Taureza Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dara Juliana Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Dita Audia Putri Djarot Sasongko Hami Seno Dyah Sulistyani Rahayu Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Farras Agung Binukajati Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hanifah Aryani Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I MADE ARTIKA I Made Artika Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI Latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa Maria Bintang MARIA BINTANG Maria Magdalena Maulana, Asy Syifa Tickna Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Renza Fajriansyah Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novian Liwanda Novian Liwanda Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raihan Permana Putra Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Arianti Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Stephanie Sriwongo Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Teti Mulyati Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti