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All Journal International Journal of Advances in Applied Sciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Hemera Zoa Vegetalika VALENSI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Chemistry Agrin : Jurnal Penelitian Pertanian Justek : Jurnal Sains Dan Teknologi Biosaintifika: Journal of Biology & Biology Education Media Farmasi Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia YARSI Medical Journal Poltekita : Jurnal Ilmu Kesehatan Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Contemporary Multidisciplinary Research Indonesian Journal of Jamu

Waras Nurcholis

Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (IPB University), Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 334416

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Optimization of Extraction Solvent for Analysis of Total Phenolic and Antioxidant Activity of Guava Leaves (Psidium guajava) using Simplex Centroid Design Damarhati, Lelly Aulia; Fajriansyah, Muhamad Renza; Anyalirria; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.344

Guava (Psidium guajava) is a popular tropical plant widely used as processed food and medicine for various diseases. This plant contains highly bioactive compounds like quercetin, flavonoids, and ferulic acid. The bioactive content can be influenced by the extraction method. However, the solvent used for extraction can also affect the bioactive content. Therefore, this study aims to determine the best solvent or combination of extraction solvents to extract guava leaves based on total phenolic content (TPC) and antioxidant activity using simplex centroid design (SCD). This research used guava leaf samples with various solvent combinations of water, ethanol, and acetone. The determination of phenolic content was determined using the TPC method, while the determination of antioxidants was conducted using the FRAP and ABTS methods. The research results showed that the highest TPC levels were produced by the solvent water : ethanol : acetone. Then the highest levels of FRAP and ABTS antioxidants were produced by the water: ethanol solvent. The solvent optimization solution obtained was using water: ethanol: acetone with a ratio of 0.462:0.436:0.102, respectively.

Vegetation Association of Bird of Paradise Community in Berber Village Forest, West Bonggo District, Sarmi Regency, Papua Province Indonesia Raunsay, Edoward Krisson; Koirewoa, Dolfina Costansah; Jesajas, David Reinhard; Nurcholis, Waras
Indonesian Journal of Contemporary Multidisciplinary Research Vol. 3 No. 6 (2024): November 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/modern.v3i6.12272

This research aims to determine the richness, diversity and dominance of tree and bird species, as well as analyzing the relationship between trees and bird communities in the Berber Village forest. Data collection methods include observation, exploration and Concentration Counts. Species richness analysis uses the Margalef Index. Diversity index, with the Shannon-Wiener equation, Dominance index using the dominance index. Assessment of the ecological function of trees using four assessment categories (very good, good, moderate and poor). A total of 36 individuals were observed, consisting of 23 males and 13 females. The richness of birds of paradise in Berber village forests is in the low category (0.4343) and is dominated by P. minor. Activity P. minor observed at various heights ranging from 21 to 64 meters above sea level (masl). Distribution of Bird of Cenderawasih activity (P. minor And C. king) shows a predilection for certain tree types and variations in height.

Analisis Kemiripan 20 Aksesi Temu Ireng (Curcuma aeruginosa Roxb.) Berdasarkan Warna Rimpang, Hasil Ekstrak, dan Kandungan Fitokimia Nurcholis, Waras; Khumaida, Nurul; Syukur, Muhamad; Bintang, dan Maria
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTCurcuma aeruginosa Roxb., popularly known as “temu ireng”, is considered as a potential source of medicinal plant for pharmacological activities. However, varieties of C. aeruginosa are still limited in Indonesia so it needs more accessions for improvement and development of new varieties. Rhizome colors, phytochemical contents and extract yield from 20 promising lines of C. aeruginosa were investigated by qualitative method for rhizome colors and phytochemical contents, and maceration method using 70% ethanol for yield extract. Similarity analysis was used for cluster analysis based on rhizome colors, phytochemical contents and yield extract. Blue was the color characterization of rhizome C. aeruginosa. The extract yield for 20 promising lines of C. aeruginosa varied from 7.92 to 19.71%, with KN and BH promising lines having the lowest and highest value, respectively. All promising lines of C. aeruginosa contain saponin and triterpenoid. Based on similarity analysis, all promising lines could be divided into 3 clusters. Cluster I consisted of 14 promising lines i.e. WG, SH, KA, GD, BH, KP, NW, PW, MB, PR, PT, KN, MD, and PK. Cluster II consisted of 4 promising lines i.e. LC, CB, KL, and GK. Cluster III consisted of 2 promising lines i.e. KD and SG. Keywords: promising lines, saponin, triterpenoid

Evaluasi Karakter Agro-morfologi Jengger Ayam (Celosia cristata L.) pada Genotipe Mutan M3 Nella Angelina Simanjuntak; Syarifah Iis Aisyah; Nurcholis, Waras
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Celosia cristata L. atau jengger ayam merupakan tanaman hias dengan bentuk yang unik. Peningkatan keragaman C. cristata melalui teknik hibridisasi terkendala karena bunganya berbentuk bulir dan berukuran kecil sehingga menyulitkan proses kastrasi dan isolasi organ reproduksi. Induksi mutasi merupakan salah satu cara yang dapat digunakan untuk meningkatkan keragaman. Tujuan penelitian ini adalah mendapatkan informasi keragaman karakter agro-morfologi pada 17 genotipe mutan M3 jengger ayam hasil induksi mutasi sinar gamma di kebun percobaan Sabisa Farm. Karakter kualitatif dan kuantitatif yang terkait dengan agro-morfologi diamati berdasarkan deskriptor Union for The Protection of New Varieties of Plants (UPOV) yang dimodifikasi. Analisis ragam dilakukan menggunakan SPSS 20.0, sedangkan analisis kemiripan dengan menggunakan PBSTAT-CL 2.1. Keragaman signifikan antar genotipe C. cristata (p < 0.05) terdapat pada karakter tinggi tanaman dan panjang bunga. Tiga kelompok berdasarkan karakter agro-morfologi terbentuk dari analisis kemiripan pada koefisien ketidakmiripan 32%. Hasil mengindikasikan bahwa karakter karakter agro-morfologi dapat digunakan untuk membedakan 17 genotipe mutan M3 jengger ayam yang diteliti, sehingga dapat digunakan untuk pengembangan penelitian selanjutnya dalam program pemuliaan tanaman. Kata kunci: analisis kemiripan, agro-morfologi, keragaman, pemuliaan mutasi

Induced Mutation by Colchicine in Java Cardamom (Amomum compactum Soland. ex Maton) Generation MV1 Nurul Komala; Syarifah Iis Aisyah; Nurcholis, Waras
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Kapulaga jawa merupakan komoditas penting tanaman aromatik yang menghasilkan produk metabolit sekunder. Produk metabolit sekunder kapulaga diyakini memiliki aktivitas antiinflamasi, antijamur, antibakteri dan antikanker. Usaha meningkatkan kandungan metabolit sekunder kapulaga melalui mutasi induksi dengan kolkisin perlu dilakukan untuk mendapatkan kapulaga poliploid. Tanaman poliploid dilaporkan memproduksi metabolit sekunder yang lebih tinggi. Penelitian ini bertujuan mendeskripsikan fenotipe tanaman kapulaga hasil perlakuan kolkisin dan mengidentifikasi tingkat ploidi melalui flow cytometry. Penelitian dilakukan dari bulan Juli 2021 sampai Januari 2022 di Kebun Percobaan Leuwikopo, Fakultas Pertanian, IPB menggunakan rancangan kelompok lengkap teracak (RKLT) faktor tunggal konsentrasi kolkisin dengan tiga ulangan. Tunas kapulaga direndam dalam larutan kolkisin pada konsentrasi yang berbeda (0, 0.05, 0.10, dan 0.15%) dan dishaker pada kecepatan 100 rpm selama 6 jam. Pengamatan meliputi tinggi tanaman, jumlah daun, jumlah anakan, panjang dan lebar daun, diameter batang, jumlah stomata, kehijauan daun, dan tingkat ploidi. Hasil penelitian menunjukkan kolkisin 0.1% menyebabkan malformasi daun dan kimera berupa garis memanjang pada daun. Perlakuan kolkisin dapat menghambat pertumbuhan tanaman. Kapulaga perlakuan kolkisin memiliki tinggi tanaman lebih pendek selama 16 minggu pengamatan, jumlah daun lebih sedikit saat 10-18 MST, jumlah anakan lebih sedikit saat 14-18 MST. Perlakuan kolkisin tidak menghasilkan poliploid berdasarkan identifikasi flow cytometry. Kata kunci:fenotipe, flow cytometri, tingkat ploidi

Analysis of Starch, Chlorophyll, and Antioxidant Content of Cardamom (Amomum cardamomum) Leaves from Various Accessions Lelly Aulia Damarhati; Sriwongo, Stephanie; Taureza, Aulia; Mulyati, Teti; Liwanda, Novian; Nurcholis, Waras
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.44781

Cardamom (Amomum cardamomum) is a herbal plant with various phytochemical compounds such as phenols, starch, tannins, terpenoids, flavonoids, and antioxidants. Cardamom of the same type but originating from different regions can produce variations in the content of metabolites. This study aimed to select cardamom accession containing high starch, chlorophyll, and antioxidant capacity. The research was conducted at Biopharmaca Experimental Garden, Cikabayan IPB University from March to August 2022 using four cardamom accessions, i.e., Bogor Hijau, Bogor Merah, Ciamis and Sukabumi. Three leaves were taken for each accession, with three replications. The leaves taken were numbers 5, 6, and 7 counted from the tops of the cardamom plant. The methods used in this study were the phenol-sulfuric acid method, the DMSO method, the DPPH method, and the CUPRAC method. The results showed that the highest starch content was produced by the cardamom of Bogor Merah accession, with an average of 3.63 g (100 g)-1 FW. The highest total chlorophyll content was found in the cardamom of Bogor Hijau accession, with an average of 1.9 mg g-1 FW. At the same time, the highest antioxidant capacity using the DPPH method was produced by the cardamom of the Sukabumi accession with an average of 1.28 μmol TE g-1 FW. In comparison, the highest antioxidant capacity of the CUPRAC method was produced by the cardamom of the Bogor Hijau accession with an average of 5.71 µmol TE g-1 FW. Thus, it is recommended to use Bogor Merah dan Bogor Hijau accession for further evaluation. Keywords: accession, antioxidant activity, cardamom leaves, chlorophyll, starch

Improvement of iron content, total phenolic, and antioxidant activity of green spinach (Amaranthus tricolor L.) with maggot fertilizer Andrianto, Dimas; Nurcholis, Waras; Ghifari, Muhammad Nabil
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.50063

Green spinach (Amaranthus tricolor L.) is a plant that contains various vitamins, such as vitamins A, B, and C, as well as protein and fiber. Green spinach contains high iron. Besides that, spinach also contains secondary metabolites such as flavonoids, antioxidants, and phenolics, which are beneficial for degradative diseases. This study aimed to evaluate the impact of plant height, iron content, total phenolic content, and antioxidant activity in spinach plants using a 70% ethanol solvent. The samples were extracted through a maceration method in a microwave for 3 minutes, followed by filtration to obtain the test sample filtrate. Iron content was determined using the ICP-OES method, total phenolic content (TPC) was measured with the Folin-Ciocalteu method, and antioxidant activity was assessed using the DPPH method. The findings revealed that treatment 2 (2 parts maggot fertilizer to 8 parts soil) had the highest iron content and antioxidant activity, whereas treatment 1 (1 part maggot fertilizer to 9 parts soil) showed the greatest plant growth and phenolic content. Keywords: allelochemical; bioherbicide; inhibitory effect; weed control

Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I Made Artika I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI latifah K Darusman Latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa MARIA BINTANG Maria Bintang Maria Magdalena Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti

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