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All Journal International Journal of Advances in Applied Sciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Hemera Zoa Vegetalika VALENSI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Chemistry Agrin : Jurnal Penelitian Pertanian Justek : Jurnal Sains Dan Teknologi Biosaintifika: Journal of Biology & Biology Education Media Farmasi Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia YARSI Medical Journal Poltekita : Jurnal Ilmu Kesehatan Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Contemporary Multidisciplinary Research Indonesian Journal of Jamu
Waras Nurcholis
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (IPB University), Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : VALENSI

 1 
Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants Waras Nurcholis; Bambang Pontjo Priosoeryanto; Edy Djauhari Purwakusumah; Takeshi Katayama; Toshisada Suzuki
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.4, Mei 2012
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.104 KB) | DOI: 10.15408/jkv.v2i4.267

Abstract

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.
Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610 Popi Asri Kurniatin; Laksmi Ambarsari; Annisa Dhiya Athiyyah Khanza; Inda Setyawati; Djarot Sasongko Hami Seno; Waras Nurcholis
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.755 KB) | DOI: 10.15408/jkv.v6i1.9440

Abstract

Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not been conducted. The purpose of this research, therefore, is to identify and characterized the gene responsible for encoding glucose oxidase, in the aspect of sequence, length, and restriction patterns. This experiment involved the amplification of genomic DNA using specific primers for gene recognition, which was followed by the restriction technique with EcoRI and PstI endonucleases. Furthermore, the gene is inserted into vector pGEM®T-Easy and transformed into competent E. coli DH5α cells, in an attempt to perform sequencing. The glucose oxidase gene from A. niger IPBCC 08.610 was confirmed to possess a size of 1848 bp, and a GC content of 57.8%, with a possibility of restriction into two fragments of size 908 bp and 980 bp, using the EcoRI restriction.
TLC Fingerprint Analysis and Evaluation of α-Glucosidase Inhibitory and Free Radical Scavenging Activity of Justicia gendarussa from Different Growth Locations Wulansari, Laela; Wahyuni, Wulan Tri; Nurcholis, Waras; Rafi, Mohamad
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.43662

Abstract

Justicia gendarussa is known to have antioxidant and antidiabetic properties, which are related to the composition and concentration of its metabolites and influenced by differences in growth location. This study aims to evaluate changes in metabolite profiles and biological activities, such as the inhibition of α-glucosidase and free radical scavenging in J. gendarussa genotypes from Bogor, Cianjur, and Sukabumi. Changes in metabolite profiles were assessed using thin-layer chromatography (TLC) fingerprint analysis combined with principal component analysis (PCA). Bioautography TLC using DPPH was performed to confirm the presence of antioxidant compounds. In the TLC fingerprint analysis of J. gendarussa, we obtained 14 bands with good resolution. PCA successfully grouped J. gendarussa extracts based on growth location. The percentage of α-glucosidase inhibitory and free radical scavenging activity was significantly different, with the highest percentage of inhibition of α-glucosidase shown by the IIIS genotype (99.23%) and the highest free radical scavenging (91.35%) demonstrated by the IIB genotype. The results of TLC bioautography confirmed the presence of antioxidant compounds in J. gendarussa represented by bands with Rf 0.04, 0.32, and 0.80. This study concludes that growth location differences influence the variations in metabolite profiles, α-glucosidase inhibitory activity, and free radical scavenging activity of J. gendarussa genotypes.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I MADE ARTIKA I Made Artika Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI Latifah K Darusman latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa Maria Bintang MARIA BINTANG Maria Magdalena Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti