Claim Missing Document
Check
Articles

Jamu as Indonesian Cultural Heritage and Modern Health Innovation Nurcholis, Waras; Arianti, Rini
Jurnal Jamu Indonesia Vol. 9 No. 1 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i1.317

Abstract

Optimization of Cardamom Fruit (Amomum compactum) Extraction Focused on Total Terpenoid Extraction and Cytotoxic Activity Using Response Surface Methodology Juliana, Dara; Priosoeryanto, Bambang Pontjo; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.328

Abstract

Cardamom is one of the medicinal plants called perennial herbs from the Zingiberaceae family. Cardamom, with the scientific name Amomum compactum, contains various phytochemical compounds such as terpenoids, flavonoids, phenolics, tannins, sterols, and proteins that are pharmacologically useful as an anticancer, antioxidant, and have cytotoxic activity. Testing the total content of terpenoid compounds that affect the cytotoxic activity of A. compactum fruit using Box-Behnken Design with three independent variables (solvent ratio, ethanol concentration, and extraction time) has never been studied before. Thus, this study aimed to obtain the extraction optimization conditions on the total terpenoid content and cytotoxic activity using Artemia salina Leach larvae with the response surface method, namely Box-Behnken Design with Design Expert 13.0 application. Determination of the total terpenoid content of the A. compactum fruit extract was carried out based on the standard ursalic acid, and the cytotoxic activity was carried out using the Brine Shrimp Lethality Test (BSLT) method. The maximum total terpenoid obtained was 4.8970 mg UAE/g DW, and the minimum IC50 value was 38.8813 ppm. Optimal conditions for the extraction of A. compactum fruit obtained a combination of 1:8 mL/g solvent ratio, 50% ethanol concentration, and extraction time of 1 day with a desirability value of 0.772. Furthermore, the optimal extraction solution results from the Box-Behnken Design were verified and analyzed using a One-Sample T-Test. Using the Box-Behnken Design extraction optimization method, the content of terpenoid bioactive compounds with cytotoxic activity from A. compactum fruit can be optimally obtained.
Potential of Sunda Porcupine (Hystrix javanica) Quills Extract as a Wound Healing Medicine: A Review of the Bioactive Components and Mechanisms Yulianto; Nurcholis, Waras; Artika, I Made
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.329

Abstract

Sunda porcupine (Hystrix javanica), a native species of Indonesia, is renowned for its distinctive sharp quills enveloping its body. Despite its reputation as a spiky creature, the use of porcupine quills in traditional medicine in Indonesian society, especially for toothache and stomach ulcers, shows that there are benefits as an alternative to herbal medicine that has yet to be fully explored. Even outside Indonesia, North American porcupine quills are reported to have antibiotic properties related to the free fatty acids that coat the spines. Extracts of Sunda porcupine quills have also shown interesting antibacterial activity. Several bioactive compounds have been found in Sunda porcupine quills extract, including alkaloids, flavonoids, saponins, steroids, triterpenoids, and peptides. These compounds have various health benefits, such as analgesic, anti-inflammatory, and antimicrobial. In addition, the antibacterial activity test on the extract showed strong potential against the growth of gram-positive bacteria. The activities of secondary metabolites that play a role in helping wound healing are anti-inflammatory, antioxidant, and antibacterial/antimicrobial activities. Wound healing mechanisms involve several complex interactions of various cellular and molecular processes. Recent research has revealed the great potential of extracts from Sunda porcupine quills as a wound healing medicine. Although the potential of Sunda porcupine quills extract as a wound healing medicine has attracted attention, this research still has limitations. Critical measures, such as in vivo and in vitro testing, still need to be improved, leaving gaps in information that require further research. The challenge of an in-depth understanding of the activity and mechanisms of bioactive compounds is the focus on optimizing the utilization of Indonesia's natural potential. This article reviews the bioactive components of Sunda porcupine quills extract and their mechanism as a wound healing agent.
Effect of Starfruit Extract (Averrhoa bilimbi Linn) Using a Combination of Water, Acetone, and Methanol Solvents on Phenolic Content and Antioxidant Capacity Putri, Dita Audia; Gultom, Milanda Fiorella; Binukajati, Farras Agung; Maulana, Asy Syifa Tickna; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.343

Abstract

Starfruit leaves, which belong to the Averrhoa family and contain tannin, saponin, flavonoid, and terpenoid compounds, have been widely used in traditional medicine due to their pharmacological benefits. This study aims to investigate the effect of solvent maceration on the extraction of total phenolics from starfruit leaves and to evaluate their antioxidant activity capacity. The study utilized Wuluh starf leaves obtained from Biofarmaka IPB University, Bogor, Indonesia, in February 023. The total phenolic content (TPC) and antioxidant tests, such as ABTS and FRAP, were influenced by the maceration stage using solvents, such as a combination of water, ethanol, and methanol. The Folin-Ciocalteu reagent and sodium carbonate were used to determine the total phenolic content, and antioxidant tests, such as the reaction with radical cations (ABTS) and the antioxidant reaction with reduction of ferri-trpyridytriazine (FRAP), were used to evaluate the antioxidant activity capacity. The results indicate that extraction with 100% acetone solvent has the FRAP method's highest phenolic content and antioxidant capacity, with 10.6325 mg GAE/g DW and 34.26641 ± 1.82 µmol TE/g DW, respectively. On the other hand, the 50:50 ethanol:acetone solvent had the highest antioxidant activity using the ABTS method, with 100.31 ± 4.80 µmol TE/g DW. The TPC-FRAP standard curve shows a positive correlation with R2 = 0.9388, while the TPC-ABTS and FRAP-ABTS standard curves do not significant correlation with the R2 values of -0.09045 and 0.08038, respectively.
Optimization of Extraction Solvent for Analysis of Total Phenolic and Antioxidant Activity of Guava Leaves (Psidium guajava) using Simplex Centroid Design Damarhati, Lelly Aulia; Fajriansyah, Muhamad Renza; Anyalirria; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.344

Abstract

Guava (Psidium guajava) is a popular tropical plant widely used as processed food and medicine for various diseases. This plant contains highly bioactive compounds like quercetin, flavonoids, and ferulic acid. The bioactive content can be influenced by the extraction method. However, the solvent used for extraction can also affect the bioactive content. Therefore, this study aims to determine the best solvent or combination of extraction solvents to extract guava leaves based on total phenolic content (TPC) and antioxidant activity using simplex centroid design (SCD). This research used guava leaf samples with various solvent combinations of water, ethanol, and acetone. The determination of phenolic content was determined using the TPC method, while the determination of antioxidants was conducted using the FRAP and ABTS methods. The research results showed that the highest TPC levels were produced by the solvent water : ethanol : acetone. Then the highest levels of FRAP and ABTS antioxidants were produced by the water: ethanol solvent. The solvent optimization solution obtained was using water: ethanol: acetone with a ratio of 0.462:0.436:0.102, respectively.
Editorialboards Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 4 No. 2 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i2.133

Abstract

Puji dan syukur kami panjatkan kepada Tuhan Yang Maha Esa atas rahmat dan hidayahNya, Jurnal Jamu Indonesia Volume 4 No 2 tahun 2019 dapat diterbitkan. Jurnal Jamu Indonesia merupakan media untuk diseminasi hasil penelitian yang berkaitan dengan jamu di Indonesia. Jamu telah dikenal lama penduduk Indonesia sebagai salah satu pengobatan. Berbagai tanaman di Indonesia sangat berpotensi sebagai jamu. Penelitian tanaman obat dari bagian daun, batang, dan rimpang tanaman telah banyak dilakukan dan terus berkembang. Naskah dalam edisi ini membahas analisis ramuan jamu antihipertensi, analisis performa pertumbuhan dan produktivitas daun duduk (Desmodium triquetrum (L.) D.C.) terhadap ketinggian tempat, khasiat antihiperglikemia daun kelor, khasiat antimikroba tanaman paku, dan khasiat jamu antidiabetes secara in vivo pada tikus. Redaksi mengucapkan terima kasih kepada seluruh penulis atas partisipasinya dalam berbagi hasil penelitiannya melalui publikasi di Jurnal Jamu Indonesia. Redaksi juga mengucapkan terima kasih kepada seluruh reviewer atas partisipasinya dalam meningkatkan kualitas tulisan naskah jurnal di edisi ini. Semua hasil karya tersebut akan diarsipkan sebagai dokumentasi berharga demi terjaganya informasi yang dapat menjadi sumber pengembangan ilmu pengetahuan di masa depan. Kontribusi publikasi peneliti-peneliti dan pejuang Jamu Indonesia terus kami harapkan untuk dapat disampaikan di dalam Jurnal Jamu Indonesia ini. Peningkatan kontribusi jumlah dan kualitas tulisan yang telah dan akan dimasukkan akan dapat membantu pengembangan jurnal ini, dan pada saatnya diharapkan juga dapat menjadikan Jurnal Jamu Indonesia menjadi referensi baik secara nasional, maupun internasional. Juni 2019Waras NurcholisWakil Dewan Redaksi
Dynamics of Nutrient Concentrations, Endogenous Hormones, Photosynthetic Capacity, and Phenological Changes in Black Orchid (Coelogyne pandurata Lindl.) from the Vegetative to Generative Phase Heriansyah, Pebra; Aziz, Sandra Arifin; Sukma, Dewi; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.215-234

Abstract

The black orchid (Coelogyne pandurata Lindl.) is one of the native Indonesian orchids from Borneo island. This study analyzed the dynamics of pigments, photosynthetic capacity, endogenous hormones, nutrient concentrations, and phenology across the vegetative to generative stages of the black orchid. The plant materials are one-year-old plants after splitting from the mother plants. Endogenous hormones, nutrient and pigment concentrations, and leaf photosynthetic capacity were measured during vegetative and generative phases. Chlorophyll, anthocyanins, and carotenoids were analyzed using UV-VIS spectrophotometry, Nitrogen (N) was analyzed by the Kjeldahl method, phosphorus (P) and potassium (K) by the Bray 1 method and Flame Photometry, and organic carbon by the Walkley- Black method with titration. The hormone levels were analyzed using HPLC, and photosynthetic capacity was determined using a Li-Cor 6800 system. Phenological changes in leaves, bulbs, and flowers were recorded. Results showed total chlorophyll increased from 1.96 to 2.36 mg/g from the vegetative to the generative stage while carotene slightly declined. Leaf nitrogen rose from 1.54% to 1.70%, bulb nitrogen decreased from 0.67% to 0.45%, whereas the C/N ratio increased from 65.24 to 85.36 from the vegetative to the generative phase. Flower nitrogen was 1.50%, and phosphorus was 0.17%. IAA in leaves decreased from 25.12 to 21.37 ng.g⁻¹ dry weight post-flowering, while gibberellin in bulbs increased from 12.28 to 12.96 ng.g⁻¹ dry weight. Zeatin in bulbs declined from 10.52 to 8.81 ng g⁻¹ dry weight. Photosynthesis peaked at 2.73 μmol. m⁻².s⁻¹ in early generative stages and then declined. Photosynthetic photon flux density and stomatal conductance decreased, reducing water and CO₂ exchange efficiency, while net assimilation and transpiration rates showed no significant changes. These physiological adjustments, including increased chlorophyll levels, changes in nitrogen allocation, and fluctuations in hormone concentrations, reflect the plant’s adaptive strategies to meet higher energy demands during reproductive growth, ensuring efficient resource distribution for flowering.
The Effect of Shade and Harvest Age on Biomass and Patchouli Oil Production in the Lowlands Riti, Dominggus Ngongo; Kurniawati, Ani; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.426-436

Abstract

This study investigated the effects of different shade levels and harvest ages on the growth, biomass yield, and essential oil production of the patchouli (Pogostemon cablin Benth.) cultivar Sidikalang, cultivated in a lowland environment. The experiment was conducted in Bogor from August 2023 to February 2024, using a nested design with three shade treatments (no shade, 55%, and 75%) and three harvest ages (4, 5, and 6 months). The results indicated that 75% shade significantly enhanced vegetative growth, including plant height, number of secondary branches, and leaf production, suggesting that this level of shading is beneficial for patchouli cultivation in lowland areas. However, these effects were not significantly different from those observed under 55% shade. The highest essential oil yields were obtained from patchouli plants grown under 75% shade and harvested at 4 months, as well as from those grown under full sunlight and harvested at 6 months.
TLC Fingerprint Analysis and Evaluation of α-Glucosidase Inhibitory and Free Radical Scavenging Activity of Justicia gendarussa from Different Growth Locations Wulansari, Laela; Wahyuni, Wulan Tri; Nurcholis, Waras; Rafi, Mohamad
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.43662

Abstract

Justicia gendarussa is known to have antioxidant and antidiabetic properties, which are related to the composition and concentration of its metabolites and influenced by differences in growth location. This study aims to evaluate changes in metabolite profiles and biological activities, such as the inhibition of α-glucosidase and free radical scavenging in J. gendarussa genotypes from Bogor, Cianjur, and Sukabumi. Changes in metabolite profiles were assessed using thin-layer chromatography (TLC) fingerprint analysis combined with principal component analysis (PCA). Bioautography TLC using DPPH was performed to confirm the presence of antioxidant compounds. In the TLC fingerprint analysis of J. gendarussa, we obtained 14 bands with good resolution. PCA successfully grouped J. gendarussa extracts based on growth location. The percentage of α-glucosidase inhibitory and free radical scavenging activity was significantly different, with the highest percentage of inhibition of α-glucosidase shown by the IIIS genotype (99.23%) and the highest free radical scavenging (91.35%) demonstrated by the IIB genotype. The results of TLC bioautography confirmed the presence of antioxidant compounds in J. gendarussa represented by bands with Rf 0.04, 0.32, and 0.80. This study concludes that growth location differences influence the variations in metabolite profiles, α-glucosidase inhibitory activity, and free radical scavenging activity of J. gendarussa genotypes.
Perbandingan Aktivitas Antioksidan dan Kandungan Fenolik Temulawak dan Temu Ireng Nurcholis, Waras; Bintang, Maria
Jurnal Jamu Indonesia Vol. 2 No. 1 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i1.27

Abstract

Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan dan kandungan fenolik dari rimpang temulawak dan temu ireng. Aktivitas antioksidan ditentukan dengan menggunakan metode DPPH (1, 1-diphenyl-2-picrylhydrazyl). Kandungan fenolik total ditentukan dengan menggunakan metode Folin-Ciocalteu. Ekstrak etanol 70% dari rimpang temulawak memiliki aktivitas antiosidan (IC50, 167.03 µg/ ml) yang lebih baik dibandingkan dengan ekstrak etanol 70% rimpang temu ireng aeruginosa (IC50, 406.52 µg/ ml). Kandungan fenolik total dari ekstrak etanol 70% rimpang temulawak (139.16 mg TAE/ g) lebih tinggi dibandingkan dengan ekstrak etanol 70% rimpang temu ireng (51.49 mg TAE/ g). Terdapat korelasi yang kuat antara aktivitas antioksidan dan kandungan fenolik total dari ekstrak temulawak dan temu ireng.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I MADE ARTIKA I Made Artika Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI Latifah K Darusman latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa Maria Bintang MARIA BINTANG Maria Magdalena Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti