Claim Missing Document
Check
Articles

POTENSI SENYAWA ANTIOKSIDAN DARI TANAMAN KROKOT (PORTULACA GRANDIFLORA): NARRATIVE REVIEW Waras Nurcholis; Riyan Alifbi Putera Irsal; Rara Annisaur Rosyidah; Muhammad Riyo Agung Kurnia; Syarifah Iis Aisyah
Jurnal Farmamedika (Pharmamedika Journal) Vol 8 No 1 (2023): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v8i1.192

Abstract

Krokot (Portulaca grandiflora) merupakan tanaman hias yang belum banyak diketahui dan dimanfaatkan secara optimal oleh masyarakat. Tanaman ini juga bermanfaat sebagai obat dan kosmetik (bahan baku). Krokot (P. grandiflora) mengandung beragam metabolit sekunder, antara lain alkaloid, flavonoid, tanin, dan lainnya. P. grandiflora diketahui memiliki potensi sebagai antioksidan. Aktivitas antioksidan dari salah satu bagian untuk satu jenis tanaman dapat berbeda karena kandungan fitokimia dari setiap bagian tanaman juga berbeda. Review ini menjelaskan kandungan fitokimia dan kadar antioksidan dari tanaman P. grandiflora dari artikel ilmiah. Artikel yang dituju bersumber dari jurnal internasional, jurnal nasional terakreditasi sinta. Terdapat banyak kandungan fitokimia dari P. grandiflora dan berpotensi bekerja sebagai antioksidan. Berdasarkan artikel dan senyawa fitokimia yang didapat tanaman P. grandiflora berpotensi sebagai antioksidan.
Uji Toksisitas Tiga Varietas Temulawak (Curcuma xanthorrhiza Roxb) sebagai Kandidat Antikanker dengan Metode BSLT (Brine Shrimp Lethality Test) Waras Nurcholis
Agrin Vol 27, No 1 (2023): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2023.27.1.728

Abstract

Temulawak merupakan tumbuhan dengan nilai ekspor tertinggi. Temulawak juga dapat tumbuh serta beradaptasi di berbagai habitat. Temulawak bermanfaat sebagai antiinflamasi dan ekstrak kurkuminoid temulawak berpotensi sebagai antikanker. Penelitian ini bertujuan untuk mengetahui efek toksisitas dan kandungan fitokimia dari tiga (3) varietas temulawak, yaitu Cursina 1, Cursina 2, dan Cursina 3. Metode yang digunakan untuk uji toksisitas secara kuantitatif yaitu dengan brine shrimp lethality test (BSLT). Senyawa yang toksik berdasarkan metode BSLT seringkali berkorelasi positif sebagai antikanker. Uji toksisitas dalam penelitian ini ditentukan oleh nilai LC50 atau mortalitas larva udang yang telah terpapar sampel selama 24 jam. Selain itu, dilakukan uji skrining fitokimia secara kualitatif. Skrining Fitokimia adalah cara mengidentifikasi bioaktif yang belum tampak dalam suatu uji yang dapat memisahkan bahan alam yang mengandung fitokimia. Skrining ini terdiri dari uji alkaloid, fenol, flavonoid, saponin, tannin, triterpenoid dan steroid. Ketiga Ekstrak (Cursina 1, Cursina 2, dan Cursina 3) menunjukkan aktivitas ketoksikan dalam uji toksisitas jika ekstrak dapat menyebabkan kematian 50% hewan uji pada konsentrasi <1000 ppm. Hasil analisis fitokimia temulawak positif mengandung metabolit sekunder seperti alkaloid, fenol, flavonoid, saponin, tanin, dan triterpenoid.  Hal ini ditunjukkan dengan perubahan warna pada larutan sampel temulawak setelah perlakuan. Berdasarkan uji ANOVA, hasil yang signifikan memiliki nilai p<0,05. Namun data yang diperoleh tidak signifikan karena menghasilkan nilai p>0,05. Suatu ekstrak dikatakan sangat toksik jika  LC50 ≤ 30 mg/L, dikatakan toksik jika nilai LC50 ≤ 1.000 mg/L, dan dikatakan tidak toksik jika nilai LC50 >1.000 mg/L. Dapat disimpulkan bahwa sampel Cursina 3 memiliki tingkat toksisitas yang lebih besar dibandingkan Cursina 1 dan Cursina 3.
Antioxidant activity and inhibition of α-glucosidase from extract and fraction of leaves and stems of Vernonia amygdalina Siahaan Rafael; I Made Artika; Waras Nurcholis
International Journal of Advances in Applied Sciences Vol 12, No 3: September 2023
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v12.i3.pp274-284

Abstract

Previous studies have shown that bitter leaf (Vernonia amygdalina Del.) has the ability as an antioxidant and an α-glucosidase inhibitor. Still, the difference in antioxidant activity and α-glucosidase inhibition based on the leaves and stems parts have not yet been determined. The effect of flavonoid-enriched extract on antioxidant activity and inhibition of α-glucosidase has not yet been determined. This research aimed to assess the impact of flavonoid-enriched extract from the leaves and stems part of the bitter leaf. The leaves and stems part of the bitter leaf were extracted using Soxhlet apparatus with 80% methanol and then underwent successive fractionation with petroleum ether, chloroform, and ethyl acetate. The crude extract and the fraction were concentrated and followed by the determination of total flavonoid, total phenolic, antioxidant activity, α-glucosidase inhibition activity, and calculated the IC50 of α-glucosidase inhibition. This research showed that chloroform-ethyl acetate leaf fraction was the best fraction with the higher total flavonoid (24.091±0.972 mg QE/g DW), total phenolic (84.299±4.589 mg GAE/g DW), diphenylpicrylhydrazyl (DPPH) antioxidant activity (33.881 μM TE/g DW), ferric reducing antioxidant power (FRAP) antioxidant activity (312.022±1.745 μM TE/g DW) and α-glucosidase inhibition activity with an IC50 value 1.23 mg/mL.
Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine Kunto Wibisono; Syarifah Iis Aisyah; Waras Nurcholis; Sri Suhesti
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.3058

Abstract

Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology
Meta-analysis on extraction methods, pharmacological activities, and cultivation techniques of Curcuma xanthorrhiza Roxb. Rahmadansah Rahmadansah; Dyah Sulistyani Rahayu; Fadila Raisyadikara; Bambang Pontjo Priosoeryanto; Waras Nurcholis
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.44657

Abstract

Javanese turmeric (Curcuma xanthorrhiza Roxb.) is important for study due to its increasing economic value in many aspects including marker-associated compounds for curcuminoids and xanthorrhizol. This systematic review aimed to summarize and find information about C. xanthorrhiza starting from its pharmacological activity and mechanism as an anticancer, anti-inflammatory, and antioxidant extraction process, and cultivation techniques. The data were obtained from relevant journals in national and international scientific databases PubMed, Scopus, Google Scholar, Web of Science, SINTA, ScienceDirect, and Wiley Online from 2000 to 2023. Results showed that from the perspective of anti-inflammatory pharmacological activities, xanthorrhizol inhibits pain response and inflammatory response. Its antioxidant activity showed a contribution to inhibiting oxidation which also successfully inhibits cancer cell proliferation. The extraction method of xanthorrhizol and curcuminoid compounds with Ultrasonic-Assisted Extraction (UAE) 20-2000 kHz performs better than other methods. C. xanthorrhiza cultivation techniques under shading produce better rhizomes. Based on research on cultivation techniques, extraction methods, and pharmacological activities, C. xanthorrhiza has an important role in the future.
Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum Irmanida Batubara; Maily Mustofa; Wulan Tri Wahyuni; Kilala Tilaar; Waras Nurcholis; Fransiska Devy Junardy; Yogo Suro Priyadi; Erna Subroto; Saat Egra; Nevianti Zamany
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22676

Abstract

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.  The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.
Efektivitas Metode Ekstraksi DNA pada Daun Segar dan Kering dari Tanaman Obat Enny Rimita Sembiring; Rerenstradika Tizar Terryana; Yuliana Galih Dyan Anggraheni; Amalia Prihaningsih; Irmanida Batubara; Waras Nurcholis; Taopik Ridwan; Rikno Harmoko
Vegetalika Vol 12, No 3 (2023): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.78957

Abstract

Tanaman obat merupakan salah satu tanaman yang sering menjadi target studi molekuler. Pada studi molekuler, ekstraksi dan purifikasi DNA genom merupakan tahap awal yang akan menentukan analisis selanjutnya. Oleh sebab itu, dibutuhkan metode ekstraksi DNA yang mampu menghasilkan DNA dengan kuantitas dan kualitas yang baik. Tujuan penelitian ini adalah untuk mengetahui efektivitas metode ekstraksi DNA dari daun segar maupun daun yang dikeringkan dari tanaman obat. Sebanyak sembilan genotip tanaman obat yang digunakan pada penelitian ini yaitu tiga genotip Justicia gendarussa, dua genotip Orthosiphon aristatus, dan masing-masing satu genotip Adenostemma lavenia, Adenostemma platyphyllum, Adenostemma madurense, dan Curcuma xanthorrhiza. Metode ekstraksi DNA genom yang digunakan adalah metode CTAB dengan beberapa modifikasi. Hasil penelitian DNA genom dari daun segar dan kering menunjukkan kuantitas, kualitas, dan dapat teramplifikasi dengan baik menggunakan primer RAPD dan penanda DNA.
Implementation of Aseptic Dispensing for Non-Cytostatic Injectable Drugs in the Internal Medicine Inpatient Ward of 'X' Hospital, West Java Novita Sari; Ros Sumarny; Dian R. Laksmitawati; Waras Nurcholis
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 1 (2023): May
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i1.1855

Abstract

Aseptic Dispensing activity is a procedure to minimize pharmaceutical preparations from the threats of pyrogens and contaminants. This study aims to determine the implementation of aseptic dispensing for non-cytostatic injectable drugs and the sterility of intravenous mixed preparations in the Internal Medicine Inpatient Ward of 'X' hospital, West Java for the period January - February 2022. This was a descriptive observational prospective study with a cross sectional approach. Researchers made direct observations of dispensing personnel, room and equipment as well as the process of aseptic dispensing activities including the stages of preparation, mixing, storage and disposal as well as sterility test of aseptic dispensing products. Based on the study results, it was obtained that of the 150 intravenous mixed preparations collected, there were 40 activities of diluting intravenous preparations, 100 activities of packaging into ready-to-use preparations and 10 activities of mixing intravenous preparations into infusion fluids, room suitability by 74%, preparations arrangement procedure by 68%, mixing procedure by 44%, storage procedure by 100% and disposal procedure by 63%. It can be concluded that the aseptic dispensing of non-cytostatic injection drugs in the internal medicine inpatient ward of 'X' hospital, West Java was not in accordance with the guidelines, especially at the mixing procedure stage (≤50). Compliance of dispensing personnel and room cleanliness during the process of mixing intravenous preparations need to be considered. The result of the identification of contamination during  the aseptic dispensing activity of non-cytostatic injectable drugs showed that 1 in 150 samples (0.66%) was contaminated.
Evaluasi Faktor Yang Mempengaruhi Ekstraksi Rimpang Temu Ireng Berdasarkan Aktivitas Penghambatan α-Glukosidase I Made Artika; Laksmi Ambarsari; Waras Nurcholis
Jurnal Jamu Indonesia Vol. 3 No. 2 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1331.754 KB) | DOI: 10.29244/jji.v3i2.57

Abstract

Terdapat beberapa faktor yang dapat digunakan dalam ekstraksi rimpang temu ireng yang memiliki aktivitas farmakologi. Penelitian ini bertujuan untuk mengidentifikasi faktor pH, konsentrasi etanol, suhu, waktu, dan rasio cairan-padatan dalam proses ekstraksi rimpang yang memberikan kontribusi signifikan sebagai inhibitor enzim a-glukosidase. Rancangan optimasi dilakukan dengan menggunakan faktoral fraksional 2(5-1). Urutan hasil dari ekstrak yang paling berkontribusi sebagai inhibitor enzim a-glukosidase: konsentrasi etanol > rasio cairan terhadap padatan > suhu > waktu > pH. Diantara faktor yang dianalisis menunjukkan bahwa konsentrasi etanol dan interaksi antara pH dan konsentrasi etanol merupakan faktor yang signifikan sebagai inhibitor enzim a-glukosidase. Dengan demikian, rimpang temu ireng memiliki potensi sebagai anti-hiperglikemia alami.
KANDUNGAN FITOKIMIA DAN METODE UJI EKSTRAK GENDARUSSA (Justicia gendarussa Burm. F) SEBAGAI ANTI-HIV : ARTIKEL REVIEW Waras Nurcholis; Hanifah Aryani; Firmanul Hasan
Jurnal Farmamedika (Pharmamedika Journal) Vol 8 No 2 (2023): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v8i2.222

Abstract

Pencarian pengobatan alternatif terbarukan untuk HIV/AIDS terus berkembang. Salah satu pendekatan untuk pengembangan obat anti-HIV dilakukan melalui penelitian menggunakan obat herbal tradisional. Justicia gendarussa Burm. F terkenal memiliki banyak manfaat di bidang medis, seperti antibakteri, antiinflamasi, antioksidan, analgesik, antikanker, antidiabetes, dan antiviral. Sebagai antiviral, ekstrak J. gendarussa teruji aktivitasnya secara in silico dan in vitro sebagai anti-HIV. Review ini bertujuan untuk memberikan informasi terkait potensi ekstrak J. gendarussa sebagai pengobatan alternatif HIV/AIDS terbarukan serta metode yang dapat digunakan untuk mengevaluasi aktivitasnya sebagai anti-HIV. Senyawa-senyawa pada J. gendarussa yang teruji aktivitasnya sebagai anti-HIV adalah justiprocumin A dan B, patentiflorin A, dan gendarusin A. Senyawa-senyawa tersebut memiliki aktivitas anti-HIV lebih baik dibandingkan obat HIV zidovudine (AZT) dan nevirapine serta dapat menghambat isolat HIV yang resisten terhadap obat NRTI (nucleoside reverse transcriptase inhibitor) dan NNRTI (non-nucleoside reverse transcriptase inhibitor).
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I MADE ARTIKA I Made Artika Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI Latifah K Darusman latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa Maria Bintang MARIA BINTANG Maria Magdalena Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur&#039; Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti